BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.4580.p
         (324 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase [Her...   613   e-177
gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase [Her...    69   5e-13

>gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase
           [Herbaspirillum seropedicae SmR1]
          Length = 324

 Score =  613 bits (1582), Expect = e-177,   Method: Compositional matrix adjust.
 Identities = 309/324 (95%), Positives = 319/324 (98%)

Query: 1   MPSAPAYPQALQDLRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLS 60
           M SAPAYP+ALQDLRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWL+
Sbjct: 1   MTSAPAYPKALQDLRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLT 60

Query: 61  QLQEELAEWQQAHPQRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPKEM 120
           QLQEELA+WQ  H QRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPP+EM
Sbjct: 61  QLQEELAQWQAEHAQRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPQEM 120

Query: 121 LDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFK 180
           LDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFK
Sbjct: 121 LDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFK 180

Query: 181 GPLVLSGAIATGDAVLAAQAMGADFAYMGSRWLATRESNVDEAYRQAIVESTAADVVYTN 240
           GPLVLSGAIATGDAVLAAQAMGADFAYMGSRWLAT+ESNVD+AYRQAIV+STAADVVYTN
Sbjct: 181 GPLVLSGAIATGDAVLAAQAMGADFAYMGSRWLATQESNVDQAYRQAIVDSTAADVVYTN 240

Query: 241 LFTGVHGNYLKKSIVNAGLDPDNLPEADKSKMNFGSGGGSKAKAWRDIWGAGQGVGLMQD 300
           LFTGVHGNYLKKSIVNAGLDPDNLPEADK+KMNFGSGGGSKAKAWRDIWGAGQGVGLM D
Sbjct: 241 LFTGVHGNYLKKSIVNAGLDPDNLPEADKTKMNFGSGGGSKAKAWRDIWGAGQGVGLMDD 300

Query: 301 VPSVAEMVDRLEAEYRAARARLAL 324
           VP+VAEMVDRLEAEYRAAR+RLAL
Sbjct: 301 VPTVAEMVDRLEAEYRAARSRLAL 324


>gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase
           [Herbaspirillum seropedicae SmR1]
          Length = 356

 Score = 68.9 bits (167), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 76/281 (27%), Positives = 130/281 (46%), Gaps = 30/281 (10%)

Query: 58  WLSQLQEELAEWQQAHPQRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPP 117
           WL+ LQ  L E+      R   P+   + ++QS +   Q     ++H   ++      PP
Sbjct: 88  WLAHLQPLLDEF----GGRIAAPL---KEIYQSFNADTQMQQVVLEHAPAVVSFHFGLPP 140

Query: 118 KEMLDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSP--------F 169
            +++ A+   G +++    +L  A +   AG+D ++     AGGH G   P        F
Sbjct: 141 ADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHRGIFDPTKDEQIGSF 200

Query: 170 ALVGEVRKMFKGPLVLSGAIATGDAVLAAQAMGADFAYMGSRWLATRESNVDEAYRQAIV 229
           AL+  + +  + P++ +G I  G  + AA  +GA    MG+ +L TRESN + +YR A++
Sbjct: 201 ALLRLLVQHTRLPVIAAGGIMDGHGIAAALQLGAQAVQMGTAFLLTRESNANASYR-ALL 259

Query: 230 ESTAADVVYTNLFTGVHGNYLKKSIVNAGLDP-DNLPEADKSKMNFGSGGGSKAKAW--- 285
           +S  A   +T +   + G    + IVN  ++  D                G +  A    
Sbjct: 260 KSPRAQ--HTRVTAAISGRP-ARGIVNRFMEEIDTPAAPPIPAYPAAYDAGKQLHALAAA 316

Query: 286 RDI------WGAGQGVGLMQDVPSVAEMVDRLEAEYRAARA 320
           RD+      W AGQG  L++D  +VAE+V  L  E++A +A
Sbjct: 317 RDVHEFGAHW-AGQGAALIRDGYTVAELVHLLVQEWQAQQA 356


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.133    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,019,340
Number of extensions: 43749
Number of successful extensions: 199
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 196
Number of HSP's successfully gapped: 3
Length of query: 324
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 239
Effective length of database: 1,217,395
Effective search space: 290957405
Effective search space used: 290957405
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)