BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4579.p (570 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ... 496 e-141 gi|300310625|ref|YP_003774717.1| hemolysin activation translocat... 328 9e-91 gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ... 73 7e-14 >gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 584 Score = 496 bits (1278), Expect = e-141, Method: Compositional matrix adjust. Identities = 259/526 (49%), Positives = 345/526 (65%), Gaps = 13/526 (2%) Query: 51 PYVVNPVFPSTQATPLSFPEEEQPCSRIDALVLQGKAAQDFQFALSEVRQPANEGGALGR 110 P V P P A + P +E PC + +VL G + F + L AN GG R Sbjct: 66 PDVRLPAQPGAAAE--AVPAQESPCFAVHRVVLDGPGSARFDWLLDLPE--ANPGG---R 118 Query: 111 CLGGRGIQMVIARVQSAIIERGFTTTRVLVQPQDLSSGTLVLTLAPGRVRKLRMAEDADP 170 CLG +GI V++ +Q+AI+ RG+ TTR+L QPQD++SG L LT+ PG++R +R A++ADP Sbjct: 119 CLGVQGINAVMSALQNAIVARGYVTTRILAQPQDIASGELRLTIVPGKIRAIRFADNADP 178 Query: 171 RGTLLNALPLSEGDILNLRAIEQGLENLKRLPTVQADIQITPSQEADAQDGDSDLVVQYQ 230 R T NALP S GD+LNLR IEQGLENLKR+PT +AD+QI PS+ DA GDSDLV+ YQ Sbjct: 179 RATQWNALPASPGDLLNLRDIEQGLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVISYQ 238 Query: 231 QALPFRFSSSLDDGGTRASGRYQAGVTLTYDNWWTLNDLFYVSLNRSL--GRYGDRGNRS 288 Q +P R + S+DD GT+A+G+YQ T++ DNW TLNDLFY+S + L G G RG++S Sbjct: 239 QGIPIRLTLSVDDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHDLGGGDPGARGSQS 298 Query: 289 HTVHYSLPFGDWLLSATSSASRYHQTVAGAFEPVRYSGEGESGELKLSRLVQRDGSSKTS 348 VHYSLP G W L+ T+S++RYHQ VAG + YSG+ E+ ELKLSRLV RD KT+ Sbjct: 299 QVVHYSLPLGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLSRLVYRDAVRKTT 358 Query: 349 LAGKLLLRRSLNFIDGIVLGPQQRRMSAWELSVHHRHFIGSAVLEGNLGYRRALDRQGQE 408 L+ K +R S N++D + Q+RR++ WE+ + HR FIG L+ L Y+R Sbjct: 359 LSFKGFVRSSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGEGTLDLALAYKRGTGAFDAM 418 Query: 409 AQDELDLPQIVSRYALWLADAAWNVPWQ----WGSARLRYSGAARAQWNRGALPTQDQFA 464 A E + SR+ L AD N P+ WG +RY+ ARAQWNR L QD+F+ Sbjct: 419 AAPEEAFGEGTSRFRLMTADLNLNAPFALPMPWGKQAMRYTLGARAQWNRTPLTPQDKFS 478 Query: 465 IGGRYTVRGFDGELTLQAERGWLLRNEVALALGASGQEIYAGFDSGAVAGALTQNLTGRR 524 +GGRY+VRGFDGE L AERGW +RNE+A ALGAS QEIYAG D G V G T+ L GRR Sbjct: 479 LGGRYSVRGFDGESNLLAERGWYVRNELAAALGASKQEIYAGLDYGEVGGPSTELLVGRR 538 Query: 525 LSGAVLGWRGSLDKLGYELFVATPLARPAGWASASVTGGFNLQWTY 570 L+GAVLG RG+L + YE+F P+++P + ++ +T G +L W++ Sbjct: 539 LAGAVLGLRGALGRTNYEVFAGVPISQPDRFKTSPLTAGMSLMWSF 584 >gi|300310625|ref|YP_003774717.1| hemolysin activation translocator protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 328 bits (840), Expect = 9e-91, Method: Compositional matrix adjust. Identities = 202/535 (37%), Positives = 288/535 (53%), Gaps = 25/535 (4%) Query: 44 RQQLELTPYVVNP---VFPSTQATPLSFPEE-EQPCSRIDALVLQGKAAQDFQFALSEVR 99 R +L Y + P FP Q L+FPE+ + R LQ Q L+ Sbjct: 64 RSDEDLQSYTLAPESLCFP-VQRVTLAFPEQVSEAVRRTGGFALQMDPFHFIQTYLA--- 119 Query: 100 QPANEGGALGRCLGGRGIQMVIARVQSAIIERGFTTTRVLVQPQDLSSGTLVLTLAPGRV 159 G G CLG +GI+ ++ RV I+ERG++TTRV + QDLS+G L +TL PG + Sbjct: 120 ------GYAGSCLGQQGIETILRRVTGQILERGYSTTRVGIGQQDLSTGQLSITLVPGII 173 Query: 160 RKLRMAEDADPRGTLLNALPLSEGDILNLRAIEQGLENLKRLPTVQADIQITPSQEADAQ 219 +R +D + +A P G +LNLR +EQGLE +KR+ + D+QI P+ Sbjct: 174 HAIRF-KDPAITASYRSAFPTQAGALLNLRDLEQGLEQMKRVASQDVDMQILPTDVP--- 229 Query: 220 DGDSDLVVQYQQALPFRFSSSLDDGGTRASGRYQAGVTLTYDNWWTLNDLFYVSLNRSLG 279 G SD+V++ ++ P++ + SLDD G +++G+YQAG+ L D+ +NDLF + Sbjct: 230 -GQSDVVLEVKRIKPWKLTLSLDDSGAKSTGKYQAGINLAIDDLLGINDLFNAGFSSDAD 288 Query: 280 RYGD-RGNRSHTVHYSLPFGDWLLSATSSASRYHQTVAGAFEPVRYSGEGESGELKLSRL 338 R G RG + YS P G W + S SRYHQ +AG F+ SG E+ E+++ +L Sbjct: 289 RMGQARGTTGNNFSYSAPAGYWTFGVSGSTSRYHQRIAGLFQTFVSSGHSENLEVRVQKL 348 Query: 339 VQRDGSSKTSLAGKLLLRRSLNFIDGIVLGPQQRRMSAWELSVHHRHFIGSAVLEGNLGY 398 QRD K SL ++ R S ++ID + Q+R + EL HRH+ G L+ L Sbjct: 349 FQRDAQQKNSLQLRIGKRWSRSYIDDTEIEVQRRNTTFAELGWMHRHYFGDVQLDATLAN 408 Query: 399 RRALD--RQGQEAQDEL-DLPQIVSRYALWLADAAWNVPWQWGSARLRYSGAARAQWNRG 455 R + +A D L D P RY++ DA P++ G L Y G R Q +R Sbjct: 409 RWGVSWFNGAADAPDRLSDGP--TYRYSMQTIDATVVAPFKLGGQSLTYVGTLRGQHSRS 466 Query: 456 ALPTQDQFAIGGRYTVRGFDGELTLQAERGWLLRNEVALALGASGQEIYAGFDSGAVAGA 515 L DQFAIG RYTVRGFDGELTL +E G LRN++ +ALG++G +YAG D+G + G Sbjct: 467 RLYLTDQFAIGSRYTVRGFDGELTLASESGVFLRNDLEIALGSTGAALYAGLDAGRLYGP 526 Query: 516 LTQNLTGRRLSGAVLGWRGSLDKLGYELFVATPLARPAGWASASVTGGFNLQWTY 570 NL G L+GA +G RG++ L YELF L RP + +A GFNL + + Sbjct: 527 SVANLQGDTLAGAAIGLRGAVSGLHYELFAGWALVRPEQFQTAMPAAGFNLIYQF 581 >gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 569 Score = 72.8 bits (177), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 106/415 (25%), Positives = 185/415 (44%), Gaps = 49/415 (11%) Query: 109 GRCLGGRGIQMVIARVQSAIIERGFTTTRVLVQPQDLSSGTLVLTLAPGRVRKLRMAEDA 168 GR G IQ + +++ G+T +V V Q+LSSG + + + V K++++E+ Sbjct: 82 GRVYGD--IQQALEALENLYRSAGYTAVQVHVPEQELSSGEVRIDVVETVVGKVQVSENR 139 Query: 169 D-PRGTLLNALP-LSEGDILNLRAIEQGLENLKRLPTVQADIQITPSQEADAQDGDSDLV 226 + + ++P L EG NLR I + ++ P Q ++ ++ +Q D D Sbjct: 140 HFSQANIRASIPALKEGVPPNLRRISESVQLANDNPAKQVNVTLSAAQSGDQIDAK---- 195 Query: 227 VQYQQALPFRFSSSLDDGGTRASGRYQAGVTLTYDNWWTLNDLFYVSLNRSLGRYGDRGN 286 ++ + P R +LD+ G+ +GR++ GV + + N + + + V+ S Sbjct: 196 IEVKDDDPLRVMLNLDNTGSADTGRWRTGVAIQHSNLFNRDHVATVAYTTSPDSPSGSKV 255 Query: 287 RSHTVHYSLP---FGDWL--LSATSSASRYHQTVAGAFEPVRYSGEGESGELKLSRLVQR 341 +++ Y LP GD L + SS S QT+A + +G GE L+ + R Sbjct: 256 DLYSLGYRLPLYELGDSLDFIYGKSSVSS-GQTLA-LNSTLNITGRGEVYALRWNHFFAR 313 Query: 342 DG--SSKTSLAGKLLLRRSLNFIDGIVLGPQQRRM----SAWELSVHH---RHFIGSAVL 392 +G SSK L S I+G +L S LS+ + R +G V+ Sbjct: 314 NGEWSSKLVLGADYKKVDSSCAINGGLLNGATLNTCLPYSTLPLSLTYSAQRQGVGE-VI 372 Query: 393 EGNLGYRRALDRQGQEAQDELDLPQIVSRYALWL-------------ADAAWNVPWQ--W 437 + N+G R + GQ ++ D Q+ RY+L+ A+W +Q W Sbjct: 373 DANIGVARNM-ATGQARRN--DALQVTDRYSLFTNGRQSMDNFIAVRGGASWLKAFQNDW 429 Query: 438 GSARLRYSGAARAQWNRGALPTQDQFAIGGRYTVRGFDGELTLQAERGWLLRNEV 492 ++R +G Q + ALP +QF + G VRGF E + A+ G +L +E+ Sbjct: 430 ---QMRLAG--NLQLSPHALPAVEQFGLAGATAVRGFT-ERAVSADSGVVLNSEL 478 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,765,645 Number of extensions: 77866 Number of successful extensions: 243 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 236 Number of HSP's successfully gapped: 3 Length of query: 570 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 480 Effective length of database: 1,193,720 Effective search space: 572985600 Effective search space used: 572985600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)