BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4567.p (138 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313971|ref|YP_003778063.1| hypothetical protein Hsero_4689... 255 8e-70 >gi|300313971|ref|YP_003778063.1| hypothetical protein Hsero_4689 [Herbaspirillum seropedicae SmR1] Length = 136 Score = 255 bits (652), Expect = 8e-70, Method: Compositional matrix adjust. Identities = 121/138 (87%), Positives = 129/138 (93%), Gaps = 2/138 (1%) Query: 1 MSHKKDKSGPCPCGQPSFDQCCGRFIGGGQIPETAELLMRSRYSAFALRDEAYLRATWFP 60 MS+KKDK+ CPCGQPSFD+CCGRFI GGQIP TA+LLMRSRYSAFALRDEAYLRATWFP Sbjct: 1 MSNKKDKA--CPCGQPSFDKCCGRFITGGQIPATADLLMRSRYSAFALRDEAYLRATWFP 58 Query: 61 DTLPEEELTSETDVKWIGLDIKNHQHASDSDEATVEFVARFKVAGRAHRLHEISHFVRQP 120 DTLPEEELTSETDVKWIGLDIK HQH + SDEATVEFVARFKVAGRAHRLHEIS+FVRQP Sbjct: 59 DTLPEEELTSETDVKWIGLDIKKHQHVAGSDEATVEFVARFKVAGRAHRLHEISNFVRQP 118 Query: 121 DATGQLRWYYVDGRFPDD 138 DA GQ+RWYYVDG FP+D Sbjct: 119 DAAGQMRWYYVDGSFPED 136 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 481,162 Number of extensions: 18366 Number of successful extensions: 33 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 32 Number of HSP's successfully gapped: 1 Length of query: 138 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 62 Effective length of database: 1,260,010 Effective search space: 78120620 Effective search space used: 78120620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)