BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.4566.p
         (200 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate...   374   e-105
gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate...   100   7e-23
gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate...    67   8e-13

>gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 200

 Score =  374 bits (960), Expect = e-105,   Method: Compositional matrix adjust.
 Identities = 178/200 (89%), Positives = 191/200 (95%)

Query: 1   MSNPIDFYFDFSSPYGYFAAVEIDKLAARHGRSVNWHPILLGPIFKALGTNSLVNIPLKG 60
           MSNPIDFYFDFSSPYGYFAAVEIDKLAA+HGR VNWHPILLGPIFKALGTNSLVNIP+KG
Sbjct: 1   MSNPIDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALGTNSLVNIPVKG 60

Query: 61  EYSKHDMERTARFHNIFYQAPSNFPIGSQVAARATLWVQQTQPAKAVDLIKTLYSAYFTE 120
           EYS+HDMERTARFHNIFY+APS+FPIG+QVAARATLWVQQTQPAKAV+LIKTLYSAYFTE
Sbjct: 61  EYSRHDMERTARFHNIFYKAPSHFPIGTQVAARATLWVQQTQPAKAVELIKTLYSAYFTE 120

Query: 121 DIDISVTDHVLRIAADAGIDRHALEAALASPELKEQLRQRTDAAAKAGVFGSPFMIVDGE 180
           DIDISV D+VLRIAAD GIDR+AL+AAL SPELK+QLRQ T+AA +AGVFGSPFMIVDGE
Sbjct: 121 DIDISVVDNVLRIAADLGIDRNALQAALESPELKDQLRQSTEAAGQAGVFGSPFMIVDGE 180

Query: 181 AFWGFDRFTQLEAFLKNGKI 200
            FWGFDRF QLEA LKNGKI
Sbjct: 181 QFWGFDRFPQLEALLKNGKI 200


>gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 206

 Score =  100 bits (249), Expect = 7e-23,   Method: Compositional matrix adjust.
 Identities = 61/193 (31%), Positives = 94/193 (48%), Gaps = 7/193 (3%)

Query: 1   MSNP---IDFYFDFSSPYGYFAAVEIDKLAARHGRSVNWHPILLGPIFKALG--TNSLVN 55
           M+ P   I+++FDF S Y Y + + I++ A + G  V W P LLGP+F+ LG  T+  V 
Sbjct: 1   MNGPAKLIEYWFDFGSNYSYLSTMRIEQAAQQAGVRVVWRPFLLGPVFQELGWSTSPFVL 60

Query: 56  IPLKGEYSKHDMERTARFHNIFYQAPSNFPIGSQVAARATLWVQQTQPAKAVDLIKTLYS 115
              KG Y+  DM R  R + + +  PS FP  + +A R  L  +      +    + +  
Sbjct: 61  QKEKGAYTWKDMTRQCRKYGLPWTMPSRFPRAAVLATRVALSFEDALWMSS--FCRLVMH 118

Query: 116 AYFTEDIDISVTDHVLRIAADAGIDRHALEAALASPELKEQLRQRTDAAAKAGVFGSPFM 175
             F ED DI   + +  +    G+    + A   S   + +LR++T  A   G+FG+P  
Sbjct: 119 LNFAEDRDIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQARTLGIFGAPTF 178

Query: 176 IVDGEAFWGFDRF 188
            V  E FWG DR 
Sbjct: 179 FVGDEMFWGNDRL 191


>gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 201

 Score = 67.4 bits (163), Expect = 8e-13,   Method: Compositional matrix adjust.
 Identities = 58/200 (29%), Positives = 90/200 (45%), Gaps = 4/200 (2%)

Query: 1   MSNPIDFYFDFSSPYGYFAAVEIDKLAARHGRSVNWHPILLGPIFKALGTNSLVN-IPLK 59
           MS   +++F   SP+ Y        LA ++   +   P  L  IF   G   L    P +
Sbjct: 1   MSKVCEYFFAPHSPFAYLGHDRFVALADKYQVQILPKPFDLSKIFGVSGGLPLAKRAPQR 60

Query: 60  GEYSKHDMERTARF-HNIFYQAPSNFPIGSQVAARATLWVQQTQ-PAKAVDLIKTLYSAY 117
             Y   ++ R + F        P++FP+ S  AAR  +  Q     A A+ L   +  A 
Sbjct: 61  QAYRLKELARWSEFLGKPMILQPAHFPVNSDNAARLIIATQLAHGTAAALRLAGAVMRAV 120

Query: 118 FTEDIDISVTDHVLRIAADAGIDRHALEAALASPELKEQLRQRTDAAAKAGVFGSPFMIV 177
           + E  +I+  D ++ IA     D  AL  +  +  ++ +  + TD A  A VFG+P+ I 
Sbjct: 121 WEEQKNIADPDTLVAIANVQEHDGAALLKSAETASVQAEFDRFTDEANAASVFGAPWFIY 180

Query: 178 DGEAFWGFDRFTQLE-AFLK 196
           +GE +WG DR   LE AF K
Sbjct: 181 EGEGYWGQDRLDFLERAFAK 200


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.136    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 636,266
Number of extensions: 25408
Number of successful extensions: 79
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 3
Length of query: 200
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 119
Effective length of database: 1,236,335
Effective search space: 147123865
Effective search space used: 147123865
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 128 (53.9 bits)