BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4565.p (394 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 785 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 251 6e-68 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 204 1e-53 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 152 3e-38 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 105 5e-24 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 785 bits (2026), Expect = 0.0, Method: Compositional matrix adjust. Identities = 380/394 (96%), Positives = 390/394 (98%) Query: 1 MIHLPGLSFDHGDDIAALREAVAAFAQSEIAPRAAEIDRSDQFPMDLWKKLGELGVLGMT 60 MIHLPGLSFDHG+DIAALREAVAAFA SEIAPRAAEIDRSDQFPMDLWKKLG+LGVLG+T Sbjct: 1 MIHLPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT 60 Query: 61 VGEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPKL 120 V EEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLP+L Sbjct: 61 VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL 120 Query: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKQGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 ISGDFIGALAMSEPNAGSDVVSMKLRADK+GDRYVLNGSKMWITNGPDADVLVVYAKTDL Sbjct: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 Query: 181 QAGARGMTAFLVEKGFKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV 240 +AGARGMTAFLVEKG+KGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV Sbjct: 181 EAGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV 240 Query: 241 LMSGLDFERSVLSGGPLGIMQACMDVVVPYVHQRQQFGQAIGEFQLMQGKLADMYSTMMA 300 LMSGLDFERSVLSGGPLGIMQACMDVVVPYVH R+QFGQAIGEFQLMQGKLADMYSTMMA Sbjct: 241 LMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMA 300 Query: 301 CKAYVYAVGQACDRADNTDKVRALRKDAAGAILYSAEKATWMAGEAIQALGGNGYINEYP 360 CKAYVYAVGQACDRAD+ DKVRALRKDAAGAILYSAEKATWMAGEAIQ+LGGNGYINEYP Sbjct: 301 CKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYP 360 Query: 361 VGRLWRDAKLYEIGAGTSEIRRMLIGRELFADTL 394 VGRLWRDAKLYEIGAGTSEIRRMLIGRELFADTL Sbjct: 361 VGRLWRDAKLYEIGAGTSEIRRMLIGRELFADTL 394 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 251 bits (642), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 129/375 (34%), Positives = 223/375 (59%), Gaps = 6/375 (1%) Query: 18 LREAVAAFAQSEIAPRAAEIDRSDQFPMDLWKKLGELGVLGMTVGEEYGGAGLGYLAHII 77 +R+A+ FAQ + P AA+ DR+ FP + K+L ELG +GM V E++GGAG+ Y++ ++ Sbjct: 10 IRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVL 69 Query: 78 AMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPKLISGDFIGALAMSEPNAG 137 A+EEI+ + ++L ++ G+ QK ++L L G+ +G ++EP+ G Sbjct: 70 ALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTG 129 Query: 138 SDVVSMKLRADKQGDRYVLNGSKMWITNGPDADVLVVYAKTDLQAGARGMTAFLVEKGFK 197 SD +++ RA++ GD ++LNG+K +IT+G A V +V+A TD AG +G++ FLV Sbjct: 130 SDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPCDTP 189 Query: 198 GFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPL 257 GF V + +K+G S T +++ +C VP +LG G G + +S L+ R ++ + Sbjct: 190 GFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSV 249 Query: 258 GIMQACMDVVVPYVHQRQQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADN 317 G+ +A + V Y QR+ FG I E Q + +LADM + + A + V+ Q Sbjct: 250 GMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQL------ 303 Query: 318 TDKVRALRKDAAGAILYSAEKATWMAGEAIQALGGNGYINEYPVGRLWRDAKLYEIGAGT 377 D+ R K+A+ A ++++E A +A +AIQ GG GY +++PV R++RD ++ +I G Sbjct: 304 KDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGA 363 Query: 378 SEIRRMLIGRELFAD 392 ++I+R++IGR + +D Sbjct: 364 NDIQRLVIGRAIASD 378 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 204 bits (518), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 134/385 (34%), Positives = 204/385 (52%), Gaps = 9/385 (2%) Query: 7 LSFDHGDDIAALREAVAAFAQSEIAPRAAEIDRSDQFPMDLWKKLGELGVLGMTVGEEYG 66 + F+ D A +++ FA E+AP AA+ D FP+D+ K GELG G+ E G Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 67 GAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPKLISGDFI 126 G GL L + EE++RA S H N+ I G K ++ +L +G I Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIH-NMVSWMIASWGQPSLKAQWCEQLAAGRKI 119 Query: 127 GALAMSEPNAGSDVVSMKLRADKQGDRYVLNGSKMWITNGPDADVLVVYAKTDLQAGARG 186 G+ ++EP +GSD S+K A + YVLNGSK +I+ DVLVV A+T + GARG Sbjct: 120 GSYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGE-GARG 178 Query: 187 MTAFLVEKGFKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLD 246 ++A +V G S +K K+G T + F + +VP +++LG G+G M GLD Sbjct: 179 VSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLD 238 Query: 247 FERSVLSGGPLGIMQACMDVVVPYVHQRQQFGQAIGEFQLMQGKLADMYSTMMACKAYVY 306 R ++ +G QA +D Y+ +R+QFG+ I +FQ +Q KLADM + ++A + V Sbjct: 239 GGRINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVR 298 Query: 307 AVGQACDR-ADNTDKVRALRKDAAGAILYSAEKATWMAGEAIQALGGNGYINEYPVGRLW 365 D + A+ K A + + + EA+Q GG GYI EYP+ R + Sbjct: 299 LAATKLDAGSPEATTYCAMAKRLATDLGFR------VCNEALQLHGGYGYIREYPLERYF 352 Query: 366 RDAKLYEIGAGTSEIRRMLIGRELF 390 RD ++++I GT+EI R++I R+L Sbjct: 353 RDVRVHQILEGTNEIMRVIISRKLL 377 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 152 bits (385), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 120/382 (31%), Positives = 195/382 (51%), Gaps = 19/382 (4%) Query: 5 PGLSFDHGDDIAALREAVAAFAQSEIAPRAAEIDRSDQFPMDLWKKLGELGVLGMTVGEE 64 P S H D LR+AV ++ +ID + +P L + G L + +E Sbjct: 4 PTASDSHAD----LRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQE 59 Query: 65 YGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPKLISGD 124 YGG+GLG + MEEI+R + G +G N+ + R+G+ EQK +YLP++ SG+ Sbjct: 60 YGGSGLGLTEASVIMEEINRTGGNSGACHGQMYNM--GTLLRHGSPEQKERYLPRIASGE 117 Query: 125 F-IGALAMSEPNAGSDVVSMKLRADKQGDRYVLNGSKMWITNGPDADVLVVYAKT----D 179 + ++A++EP G+D +K A K+GDRYV+NG K+WI+ +D++++ A+T Sbjct: 118 LRLQSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQ 177 Query: 180 LQAGARGMTAFLVE-KGFKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGV 238 + + GM+ FLV+ K G + + L M T EL F + E+P EN++G GRG Sbjct: 178 VSRKSEGMSIFLVDLKEAIGHGMEVR-PILNMVNHETNELFFDNLEIPAENLIGEEGRGF 236 Query: 239 NVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHQRQQFGQAIGEFQLMQGKLADMYSTM 298 ++ GL+ ER++++ +G +D Y +R F + IG Q +Q LA+ Y + Sbjct: 237 RYILDGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEV 296 Query: 299 MACKAYVYAVGQACDRADNTDKVRALRKDAAGAILYSAEKATWMAGEAIQALGGNGYINE 358 A Y QAC D A A A +A+ A A +Q GG G+ E Sbjct: 297 EAANLMRY---QACALFDAQQSCGA---QANMAKYLAAKAAWEAANVCLQTHGGFGFACE 350 Query: 359 YPVGRLWRDAKLYEIGAGTSEI 380 Y V R +R+ +LY++ ++ + Sbjct: 351 YDVERKFRETRLYQVAPISTNL 372 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 105 bits (263), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 94/402 (23%), Positives = 181/402 (45%), Gaps = 37/402 (9%) Query: 7 LSFDHGDDIAALREAVAAFAQS---EIAPRAAEIDRSDQ---FPMDLWKKLGELGVLGMT 60 + F + AL+ AFA ++ P + + D+ ++ + + G + Sbjct: 3 IDFQLSESQKALQAGARAFAADVLRKVGPTITPLVKPDERFYATRPFYQAMADAGFIKAL 62 Query: 61 VGEEYGGAGLGYLAHIIAMEEISRA-----SASVGLSYGAHSNLCVNQIKRNGNEEQKRK 115 + E +GG L L +A EE++ SA +G G H+ + +G EQK++ Sbjct: 63 LPESFGGTALSTLDFALAAEELTAVDVNVPSALLGTGLGLHA------VVHHGTPEQKQR 116 Query: 116 YLPKLIS-GDFIGALAMSEPNAGSDV------VSMKLRADKQGDRYVLNGSKMWITNGPD 168 +LP I G + ALA +E G++ V ++ A +GD +V+NG+K + TNG Sbjct: 117 FLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTG 176 Query: 169 AD-----VLVVYAKTDLQ-AGARGMTAFLVEKGFKGFSVAQKLDKLGMRGSHTGELVFQD 222 D + V +TD + + +V G + LD +G R + + + F + Sbjct: 177 WDGKGAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRATISPRVHFNN 236 Query: 223 CEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHQRQQFGQ-AI 281 VP N++G G G+++L + + +++ +G+M+ D+ + + ++ G + Sbjct: 237 VRVPASNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAFDIALRFAQSDRRSGTIPV 296 Query: 282 GEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADNTDKVRALRKDAAGAILYSAEKATW 341 E Q + LAD+ M +A Y ++C + D T+ R+ A Y +E Sbjct: 297 IEHQNVGFMLADI---KMRIEAARYLTWKSCHQLDLTEGRS--RELAIITKTYCSELCVQ 351 Query: 342 MAGEAIQALGGNGYINEYPVGRLWRDAKLYEI-GAGTSEIRR 382 + +A++ +G + Y + YP+ L +DA + + G +RR Sbjct: 352 VVYDAMRVVGVDAYTDLYPLAGLMQDALCFPLYDGGNMGVRR 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,182,543 Number of extensions: 47899 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 12 Length of query: 394 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 307 Effective length of database: 1,207,925 Effective search space: 370832975 Effective search space used: 370832975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)