BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4542.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 887 0.0 gi|300310266|ref|YP_003774358.1| amidase family protein [Herbasp... 175 8e-45 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 167 2e-42 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 140 3e-34 gi|300309358|ref|YP_003773450.1| amidase family protein [Herbasp... 134 2e-32 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 887 bits (2292), Expect = 0.0, Method: Compositional matrix adjust. Identities = 454/488 (93%), Positives = 468/488 (95%) Query: 1 MHKLTLKELSAQLQARKISATELATLFLDRIEASDLNAFLHVDRALTLAQAQAADVRLAG 60 MHKLTLKELSAQLQARKISATELATLFLDRI+ASDLNAFLHVDR LTLAQA+ AD RL+ Sbjct: 1 MHKLTLKELSAQLQARKISATELATLFLDRIDASDLNAFLHVDRELTLAQAKVADSRLSD 60 Query: 61 NDAGPLTGVPIAHKDIFVTRNWRTTAGSHMLQDYVSPFDATVVEQFNAAGTVTLGKLNCD 120 N+AG LTGVPIAHKDIFVTR WRTTAGS ML +YVSPFDATVVEQFNAAG VTLGKLNCD Sbjct: 61 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCD 120 Query: 121 EFAMGSSNENSFYGPVKNPWDKTAIPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASL 180 EFAMGS NENS++GP NPWDK A+PGGSSGGSAAAVAARLAPAATATDTGGSIRQPASL Sbjct: 121 EFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASL 180 Query: 181 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPK 240 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPK Sbjct: 181 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPK 240 Query: 241 EDFTRSLNQSLQGLRIGVPREFFGAGLAADVEAAVRGALAEFEKLGATLVDISLPKTELS 300 EDFTRSLN SLQGLRIGVP+EFFGAGLAADVEAAVR AL+EFEKLGA LV+ISLPKTELS Sbjct: 241 EDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLPKTELS 300 Query: 301 IPVYYVIAPAEASSNLSRFDGVRYGHRAATYGNLDDMYKKSRAEGFGEEVKRRILVGSYV 360 IPVYYVIAPAEASSNLSRFDGVRYGHRAA YGNLDDMY+KSRAEGFGEEVKRRILVGSYV Sbjct: 301 IPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRILVGSYV 360 Query: 361 LSHGYYDAYYLQAQKIRRLIAEDFQRALAGENRQCDVIMGPVSPTVAWDLGDKTDDPVAN 420 LSHGYYDAYYLQAQKIRRLIA+DFQ ALAG NRQCDVIMGPVSPTVAWDLGDKT+DPVAN Sbjct: 361 LSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDKTNDPVAN 420 Query: 421 YLADIFTLSTSLAGLPGMSIPCGFGQGEKNAKRPVGLQIIGNYFDEARLLNVAHQFQQAT 480 YLADIFTLSTSLAGLPGMSIPCGFGQGEKN KRPVGLQIIGNYFDEARLLNVAHQFQQAT Sbjct: 421 YLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVAHQFQQAT 480 Query: 481 DWHLREPA 488 DWHLREPA Sbjct: 481 DWHLREPA 488 >gi|300310266|ref|YP_003774358.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 175 bits (443), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 151/506 (29%), Positives = 233/506 (46%), Gaps = 42/506 (8%) Query: 6 LKELSAQLQARKISATELATLFLDRIEASDLNAFLHVD------RALTLA---------- 49 LKEL++ L + TE A+ +LD ++AS +A+ +D A+ Sbjct: 11 LKELASSLHIQL--TTEQASQYLDLMQAS-FDAYDVIDGLPDEIPAVKYPRSAAARPAAK 67 Query: 50 ----QAQAADVRLAGNDAGPLTGVPIAHKDIFVTRNWRTTAGSHMLQDYVSPFDATVVEQ 105 A A + G G L G + KD GS L+ +V +DAT+V + Sbjct: 68 DNPLNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTR 127 Query: 106 FNAAGTVTLGKLNCDEFAMGSSNENSFYGPVKNPWDKTAIPGGSSGGSAAAVAARLAPAA 165 AG LGK C+ F + + S PV NP GGSS GSAA VAA A A Sbjct: 128 LLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADMA 187 Query: 166 TATDTGGSIRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAM 225 D GGSIR P++ CG G+K T+G V G++ +++D GPI D L+L + Sbjct: 188 IGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVI 247 Query: 226 TGFDERDSTSLQRPKEDFTRSLNQSLQGLRIGVPREFFGAG-LAADVEAAVRGALAEFEK 284 G D D + +T +L++ ++GL+IG+ E F G + V V+ A+A+ EK Sbjct: 248 AGADGLDPRQYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEK 307 Query: 285 LGATLVDISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAATYGNL--DDMYKKSR 342 LGA++ +S+ + L+ ++ I + + +G+ + + L + + Sbjct: 308 LGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWREH 367 Query: 343 AEGFGEEVKRRILVGSYVLSHGYYDAYYLQAQKIRRLIAEDFQRALAGENRQCDVIMGPV 402 A+ +K + VG Y L+ Y +Y +AQ I R + +ALA D+++ P Sbjct: 368 ADDLSATLKLCMFVGQYGLTR-YQGRFYAKAQNIARRARAGYDKALA----DFDLLVMPT 422 Query: 403 SPTVAWDLGDKTDDPVANYLADIF-----TLSTSLAGLPGMSIPCGFGQGEKNAKRPVGL 457 P +A L P+A Y+A F T + G P MSIPCG G PVGL Sbjct: 423 VPIIAQPL-PAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDG-----LPVGL 476 Query: 458 QIIGNYFDEARLLNVAHQFQQATDWH 483 ++G ++ EA L A F+ + DW Sbjct: 477 MLVGKHYAEATLYQAAAAFEASMDWQ 502 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 167 bits (422), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 165/486 (33%), Positives = 230/486 (47%), Gaps = 49/486 (10%) Query: 4 LTLKELSAQLQARKISATELATLFLDRIEAS-DLNAFLHVDRALTLAQAQAADVRLAGND 62 +TL E+ L R+ S E + EA DLN +L D +QA+ D + Sbjct: 6 MTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQ---GG 62 Query: 63 AGPLTGVPIAHKDIFVTRNWRTTAGSHMLQDYVSPFDATVVEQFNAAGTVTLGKLNCDEF 122 GPL+G+PIA KD +TAG+ L++ V ATVVE AG + GK N E Sbjct: 63 HGPLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHAGKANMHEL 122 Query: 123 AMGSSNENSFYGPVKNPWDKTAIPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASLCG 182 A G +N N +GP +NP+D I GGSSGG A +AARLAPAA TDTGGS+R PA+LCG Sbjct: 123 AFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCG 182 Query: 183 VTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPKED 242 V G++PT S++G++ + + D GP+A++ D LL M G RDS L Sbjct: 183 VVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAG--SRDSAPL------ 234 Query: 243 FTRSLNQSLQGLRIGVPREFFGAGLAADVEAAVRGALAEFEKLGATLVDISLPK-TELSI 301 L LR+GV R GL +VEA AL + + G LVD+ LP LS Sbjct: 235 ----LALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELVDVDLPTLAPLSH 290 Query: 302 PVYYVIAPAEASSNLSRFDGVRYGHRAATYGNLDDMYKKSRAEGFGEEVKRRI--LVGSY 359 + I E +L + + D+ K+ AE +V R I + Sbjct: 291 AAGFAIVLHEFLIDLPAY---------LQASDARDVIKRVLAEVGSPDVARIIQATLDQP 341 Query: 360 VLSHGYYDAYYLQAQKIRRLIAEDFQRALAGENRQCDVIMGPVSPTVAWDLGDKTDDPVA 419 V H Y A L+ ++ + + D R ++ D ++ P + A LG+ DD VA Sbjct: 342 VPEHAYRLA--LEQRRTMQALYRDCFR-----SQGVDALIFPTTALTARPLGE--DDTVA 392 Query: 420 NYLADIFTLS--------TSLAGLPGMSIPCGFGQGEKNAKRPVGLQIIGNYFDEARLLN 471 + T S+AGLP +SIP G + PVGL++ G + RLL Sbjct: 393 LRGERVPTFPAFARNADGASIAGLPSISIPVGLSE----YGLPVGLELDGPEGSDRRLLQ 448 Query: 472 VAHQFQ 477 VA + Sbjct: 449 VAASLE 454 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 140 bits (352), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 146/486 (30%), Positives = 236/486 (48%), Gaps = 33/486 (6%) Query: 5 TLKELSAQLQARKISATELATLFLDRIEASD-----LNAFLHVD-RALTLAQAQAADVRL 58 ++ L + A + ++ L +L RI A D LNA + ++ +AL A + R Sbjct: 53 SITHLQSAQDAGRATSRSLVLAYLARIRAYDQQGPSLNAIVTLNPKALEEADQLDRERRQ 112 Query: 59 AGNDAGPLTGVPIAHKDIFVTRNWRTTAGSHMLQDYVSPFDATVVEQFNAAGTVTLGKLN 118 +G GPL G+PI KD + T + TT G+ L + DA V++ AG V LGK Sbjct: 113 SG-PRGPLHGIPILVKDNYDTVDMPTTGGTLALATLQAQADAFQVKRLREAGAVILGKTT 171 Query: 119 CDEFAMGSSNENSFYGPVKNPWDKTAIPGGSSGGSAAAVAARLAPAATATDTGGSIRQPA 178 E A G + +S G +NP+D PGGSSGG+ AAVAA A A +DT GSIR PA Sbjct: 172 MHELAAGVTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPA 231 Query: 179 SLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSL-- 236 + + G++ T G SR G++ +S+ D P+A++ ED ++L+A G D +DS+++ Sbjct: 232 AHQNLFGLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQDSSTVDA 291 Query: 237 --QRPKEDFTRSLNQSLQGLRIGVPREFFGAGLA-ADVEAAVRGALAEFEKLGATLVDIS 293 PK SLQG RIGV R FGA A+V AA+ AL + + GA + D++ Sbjct: 292 NGHIPKSYRDGLRADSLQGARIGVLRALFGAAPEDAEVSAAINKALQQLKDQGAIVTDVT 351 Query: 294 LPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAATYGNLDDMYKKSRAEGFGEE-VKR 352 +P+ + + +I P E +L + H A G+L ++ A G E ++ Sbjct: 352 IPELDGLLSGSSII-PYEFKYDLGAY---LQSHPGAPVGSLGEIL----ARGMEHELLEA 403 Query: 353 RILVGSYVLSHGYYDAYYLQAQKIRRLIAEDFQRALAGENRQCDVIMGPVSPTVAWDLGD 412 + + + V D L+ ++R + + +N D ++ P A +G+ Sbjct: 404 GLRLRNSVDLQNPKDKEELEKVMLKRSALKSLMTDVMQKN-HLDTLVYPTIQRKAALIGE 462 Query: 413 KTDDPVANYLADIFTLSTSLAGLPGMSIPCGFGQGEKNAKRPVGLQIIGNYFDEARLLNV 472 + L ++ GLP +++P GF + PV L+++ F E LL + Sbjct: 463 PQGGAMNCQL-------SATTGLPALALPVGFTED----GLPVSLELLAPEFAEPALLGL 511 Query: 473 AHQFQQ 478 A+ ++Q Sbjct: 512 AYGWEQ 517 >gi|300309358|ref|YP_003773450.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 134 bits (337), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 142/506 (28%), Positives = 224/506 (44%), Gaps = 52/506 (10%) Query: 1 MHKLTLKE---LSAQLQARKISATELATLFLDRIEASD--LNAFLHVDRALTLA-QAQAA 54 MH+L + LSA ++++++S E+ +L+ IE + +NA + + L +A+ A Sbjct: 1 MHQLVTLDALALSAAIRSKEVSCREVMQAYLEHIEKMNPHVNAIVSLRPPEELLDEAKRA 60 Query: 55 DVRLA-GNDAGPLTGVPIAHKDIFVTRNWRTTAGSHMLQDYVSPFDATVVEQFNAAGTVT 113 D LA G G + G+P A KD+ + T+ GS + DAT V + AG + Sbjct: 61 DQLLAQGRYLGWMHGMPQAPKDLASCKGLPTSKGSPLSSMAPDQSDATCVARVRDAGAIF 120 Query: 114 LGKLNCDEFAMGSSNENSFYGPVKNPWDKTAIPGGSSGGSAAAVAARLAPAATATDTGGS 173 +GK N EF +GS++ N +G +N +D + I GGSSGG+ AA+A R+ P A +D GS Sbjct: 121 IGKTNVSEFGLGSNSYNPLFGVTRNAYDFSRIAGGSSGGAGAALAMRMLPVADGSDMMGS 180 Query: 174 IRQPASLCGVTGIKPTYGSVSR-YGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERD 232 +R PA+ V G +PT G V G F L GP+ +T D L G+D + Sbjct: 181 LRNPAAFNNVYGFRPTQGRVPYGSGGELFLQQLSTEGPMGRTVADVARLFATQAGYDPQC 240 Query: 233 STSLQRPKEDFTRSLNQSLQGLRIGVPREFFG-AGLAADVEAAVRGALAEFEKLGATLVD 291 S L + +G RIG + G + +V AL++F +G T+ Sbjct: 241 PLSSDTDVTGDAAQLKRDFKGARIGWLGNYDGYLPIEEEVMVLCEAALSDFADIGCTV-- 298 Query: 292 ISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAATYGNLDDMYKKSRAEGFGEEVK 351 E P Y + + HR G +Y F + + Sbjct: 299 ------EQCRPDY----------PMEKLWETWLVHRQWLVGGNLSVY-------FADPAR 335 Query: 352 RRILV--GSYVLSHGYYDAYYLQAQKIRR--LIAEDFQRALAGENRQCDVIMGPVSPTVA 407 R +L + + G YL A + R + D+ RA+ Q D ++ P + Sbjct: 336 RGLLKPEAQWEVESG----QYLTAADVFRASMARSDWYRAIHKLFEQFDYLLLPSAQMFP 391 Query: 408 WDLGDKTDDPVANYLADIF------TLSTSLAGLPGMSIPCGFGQGEKNAKRPVGLQIIG 461 +D+ K VA D + + +LAGLP MS+P GFG P+G+QIIG Sbjct: 392 FDVATKWPRNVAGREMDTYHRWMEVVIGPTLAGLPAMSVPVGFGAN----GLPMGMQIIG 447 Query: 462 NYFDEARLLNVAHQFQQATDWHLREP 487 D+ +L +A+ + W + P Sbjct: 448 PSRDDRGVLQLAYALEGLRPWSAQAP 473 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,465,528 Number of extensions: 61565 Number of successful extensions: 202 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 11 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)