BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4536.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum ... 838 0.0 gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase... 187 2e-48 gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidoh... 162 4e-41 >gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 838 bits (2164), Expect = 0.0, Method: Compositional matrix adjust. Identities = 401/419 (95%), Positives = 412/419 (98%) Query: 1 MDMKTQASTLRIDGARLWNSLMELAQIGGTPKGGVCRLALTDLDKQGRDLVTSWARAEGM 60 MDM TQ STLRIDGARLW+SLMELA+IG T KGGVCRLALTDLDKQGRDLV+SWARAEGM Sbjct: 1 MDMNTQISTLRIDGARLWDSLMELARIGATAKGGVCRLALTDLDKQGRDLVSSWARAEGM 60 Query: 61 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVIRTLNQHNI 120 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEV+RTLNQHNI Sbjct: 61 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVVRTLNQHNI 120 Query: 121 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLEHAYHATDVDGKNVRDELERIGYIG 180 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTL+HAY+ATDV+GK VRDELERIGYIG Sbjct: 121 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLDHAYNATDVEGKKVRDELERIGYIG 180 Query: 181 EQVCGEHPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI 240 EQVCG+HPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI Sbjct: 181 EQVCGDHPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI 240 Query: 241 RKDALQVSTYIMQEVVAIAHRYAPYGRGTVGQVQVLPNSRNVIPGQVKFSIDLRNVSEAS 300 RKDALQVSTYIMQEVVAIAHRYAPYGRGTVGQVQV+PNSRNVIPGQVKFSIDLRNVSEAS Sbjct: 241 RKDALQVSTYIMQEVVAIAHRYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEAS 300 Query: 301 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPHCVDNVRAAAAALGYKTMDVVS 360 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHP CVD VRAA+AALGYKTMDVVS Sbjct: 301 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVS 360 Query: 361 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVVTA 419 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVV+A Sbjct: 361 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVVSA 419 >gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase/N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 124/385 (32%), Positives = 189/385 (49%), Gaps = 13/385 (3%) Query: 32 KGGVCRLALTDLDKQGRDLVTSWARAEGMSVVVDQIGNIFMRRPGRNNALPPVMTGSHID 91 +G + LT+ ++ + W R GM V +D +GN+ R + ++TGSH D Sbjct: 197 EGALTCTYLTETHQRTAAQIAQWMREAGMQVHIDAVGNVVGRYQSPHPQAKTLLTGSHYD 256 Query: 92 TQPTGGKFDGNYGVLAGIEVIRTLNQHNIQTEAPIEVAVWTNEEGSRFVPVMMGSGVFCG 151 T GGK+DG G+L I V++ L++ +EV ++ EEG RF +GS G Sbjct: 257 TVRNGGKYDGREGILLPIAVVKYLHERGETLPFHLEVIGFSEEEGVRFKSTFLGSNAITG 316 Query: 152 AFTLEHAYHATDVDGKNVRDELERIGY----IGEQVCGEHPIGAYYEAHIEQGPVLENAD 207 F + D + +R+ + + G+ I + I AY E HIEQGPVL D Sbjct: 317 HFDMA-LLDLRDKEDVTMRELIIQAGHDVAAIPQIARDPAEILAYVEVHIEQGPVLLKRD 375 Query: 208 TVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEIRKDAL----QVSTYIMQEVVAIAHRYA 263 +G+V ++ G S Y + G SHAG TPM++RKDA ++ Y+ Q H Sbjct: 376 LPVGIVTSIAGSSRYLVDLKGVASHAGTTPMDMRKDAAAAAAEIILYVEQRCSQDQHAAL 435 Query: 264 PYGRGTVGQVQVLPNSRNVIPGQVKFSIDLRNVSEASLTRMHEDLLAYLEAMRKKTGLSI 323 GTVGQ+QV S NVIPG + S+D+R ++ +D+LA +E + + + + Sbjct: 436 V---GTVGQLQVPRGSTNVIPGACQLSLDIRAATDEVRLAAVKDILAKIEEICTRRSIDL 492 Query: 324 ELERVSYFPPCPFHPHCVDNVRAAAAALGYKTMDVVSGAGHDAIYAARLAPAGMIFVPCK 383 +LE P P P + + AA G ++ SGAGHDA+ RL M+F C Sbjct: 493 KLEETLSAPAAPCAPWLMAQLSAATQRAGVPPFELASGAGHDAMAMVRLTDVCMLFTRCG 552 Query: 384 D-GVSHNEIEDARPEHLEAGCNVLL 407 + G+SHN +E + E +LL Sbjct: 553 NGGISHNPLETMTADDAEVSGQILL 577 >gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 162 bits (410), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 123/402 (30%), Positives = 186/402 (46%), Gaps = 20/402 (4%) Query: 3 MKTQASTLRIDGARLWNSLMELAQIGGTPKGGVCRLALTDLDKQGRDLVTSWARAEGMSV 62 MK + G + +LAQ +P G + R LT + + W GM V Sbjct: 1 MKAVPEPFQQAGRQAMQWAQQLAQHTESP-GMLTRTYLTPQHQAAAAALARWMEEAGMRV 59 Query: 63 VVDQIGNIFMR---RPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVIRTLNQHN 119 D GN+ R PG +A P +TGSH DT GG +DGN G++ + I ++ Sbjct: 60 RRDNAGNVIGRYEAAPGHESA-PSFVTGSHFDTVRNGGWYDGNLGIVLPLACIARWHRQG 118 Query: 120 IQTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLEHAYHATDVDGKNVRDELERIGYI 179 + PIEV + EEG RF ++GS G F + D DG +R + G Sbjct: 119 QRFAFPIEVIGFAEEEGVRFKATLLGSRTVAGTFD-QAVLENRDSDGVTMRAAIRAAGLD 177 Query: 180 GEQVCGEH----PIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGP 235 + + + + A+ E HIEQGP+L N D +GVV A+ G S + V G HAG Sbjct: 178 PDGIAADAWQPGSMAAFVEVHIEQGPLLLNEDLPVGVVTAISGASRFMAEVHGLAGHAGT 237 Query: 236 TPMEIRKDAL----QVSTYIMQEVVAIAHRYAPYGRGTVGQVQVLPNSRNVIPGQVKFSI 291 PM +R+DA ++ YI + P GT+G ++V+ + NV+PG FSI Sbjct: 238 VPMHLRRDAAMAAAEIGLYIERRC-----SIKPELVGTMGLLEVVQGAANVVPGLASFSI 292 Query: 292 DLRNVSEASLTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPHCVDNVRAAAAAL 351 D+R ++ D+ A ++ + + + + L + P P + + AA Sbjct: 293 DIRAEDDSDRLAAVADVKAEIDRIAARRQVQLALRQTHEAASVPCAPRLQAALAQSIAAA 352 Query: 352 GYKTMDVVSGAGHDAIYAARLAPAGMIFVPCKD-GVSHNEIE 392 G+ + SGAGHDA+ A LA M+FV C + G+SH+ E Sbjct: 353 GWPVRHLPSGAGHDAMALAGLADVAMLFVRCGNGGISHHPDE 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,419,221 Number of extensions: 63552 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 3 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)