BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4529.p (442 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 862 0.0 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 216 3e-57 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 212 4e-56 gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 196 2e-51 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 171 1e-43 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 862 bits (2226), Expect = 0.0, Method: Compositional matrix adjust. Identities = 433/442 (97%), Positives = 438/442 (99%) Query: 1 MSSQDSSHPSSLSAYWMPFTANRNFKAAPRMLVSAEGMHYRDDAGNAILDGTAGLWCVPL 60 MSSQD +HPSSLSAYWMPFTANRNFKAAPRMLVSAEGMHYRDDAGN ILDGTAGLWCVPL Sbjct: 1 MSSQDPTHPSSLSAYWMPFTANRNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPL 60 Query: 61 GHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFAHVFYTGSGSESVD 120 GHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRF +VFYTGSGSE+VD Sbjct: 61 GHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVD 120 Query: 121 TALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLP 180 TALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLP Sbjct: 121 TALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLP 180 Query: 181 HTHNLEKNAFTKGEPEYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLK 240 HTHNLEKNAFTKGEPEYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLK Sbjct: 181 HTHNLEKNAFTKGEPEYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLK 240 Query: 241 RLREICTKHGILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVK 300 RLREICTKHGILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVK Sbjct: 241 RLREICTKHGILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVK 300 Query: 301 KEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLDNAQAVADYFQEAAH 360 EIHDAFMNGPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVL+NAQAVADYFQEAAH Sbjct: 301 PEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLENAQAVADYFQEAAH 360 Query: 361 ALRGLPHVIDVRTIGLIAGIELASLPGRVGARAYDAFVRAFNDGILIRVTGDIIALSPPL 420 ALRGLPHVIDVRTIGLIAGIELAS+PGRVGARAYDAFVRAFNDGILIRVTGDIIALSPPL Sbjct: 361 ALRGLPHVIDVRTIGLIAGIELASIPGRVGARAYDAFVRAFNDGILIRVTGDIIALSPPL 420 Query: 421 IINKQQIDELFGKLAAILKTLA 442 IINKQQIDELFGKLAAILKTLA Sbjct: 421 IINKQQIDELFGKLAAILKTLA 442 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 216 bits (549), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 143/432 (33%), Positives = 214/432 (49%), Gaps = 15/432 (3%) Query: 18 PFTANRNFKA-APRMLVSAEGMHYRDDAGNAILDGTAGLWCVPLGHAHPKIVSAVQKSVA 76 P T+ R + P +L S +G + D +LD +GLWCV G+ ++ A + + Sbjct: 17 PVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQ 76 Query: 77 TLDYAPS-FQLGHPSAFELAERLKAYTGNRFAHVFYTGSGSESVDTALKIALAYHRAKGN 135 L YA F A LA++L + HV++T GS++VD A++ YH + G Sbjct: 77 RLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAVDAAIRYITHYHHSIGK 136 Query: 136 ATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNAFTKGEP 195 + I +RGYHG G + + F+ L P +LP + +G+ Sbjct: 137 PGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPL-PTQHYLPSPYPYRSE--LQGDE 193 Query: 196 EYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHGILLIF 255 A L V A N+AA EP+ GS GV++PPKG+LK ++ C + IL + Sbjct: 194 AIIAASVQALRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNELDILFVV 253 Query: 256 DEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKKEIHDAFMNG-PDGI 314 DEVITGFGR G FA DV+PD+ T AKGLT G PMGAV + +++ +G P Sbjct: 254 DEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMST 313 Query: 315 ELFHGYTYSGHPLACAAGLASLEVFQTEGVLDNAQAVADYFQEAAHALRGLPHVIDVRTI 374 + HG TYS HP++ A L L +++ G+LDN + + F AL P V D R+ Sbjct: 314 VVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRALLAHPLVGDARSR 373 Query: 375 GLIAGIELASLPGRVGAR------AYD-AFVRAFNDGILIRVTGD-IIALSPPLIINKQQ 426 GL+ +EL + + AR +D F A+ G++ R GD I+ +P L ++ Sbjct: 374 GLLGALELVA-DKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNILGFAPALCYTQED 432 Query: 427 IDELFGKLAAIL 438 +F +L AIL Sbjct: 433 FALMFARLKAIL 444 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 212 bits (540), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 147/435 (33%), Positives = 215/435 (49%), Gaps = 19/435 (4%) Query: 17 MPFTANRNFKAAPRMLVSAEGMHYRDDAGNAILDGTAGLWCVPLGHAHPKIVSAVQKSVA 76 M +RN ++ M V+ EGM D G +D + G LGH HP+++ A++K V Sbjct: 1 MTHVFHRNPRSKLPMAVAGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVG 60 Query: 77 TLDYAPSFQLGHPSAFELAERLKAYTGNRFAHVFYTGSGSESVDTALKIALAYHRAKGNA 136 L YA + A ELA L HV++ GSE+V+ ALK+A Y+ G Sbjct: 61 ELAYAHTSFFTTAPAEELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQP 120 Query: 137 TRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNAFTKGEPE 196 R +I R + YHG G +++GG R+ F L+ H+ + A + + + Sbjct: 121 QRRHIIARRQSYHGNTLGALAIGGNAWRREMFMPMLIEA-HHVSPCYAYRNRADGESDVQ 179 Query: 197 YGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKG-YLKRLREICTKHGILLIF 255 Y LADELE+ + A + A + E V G+T +PP G Y +++R +C K+G+LLI Sbjct: 180 YVQRLADELEQKILSLGADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLIL 239 Query: 256 DEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKKEIHDAFMNGPDGIE 315 DEV++G GR G FA + V PDI AKGL G P+GA+ I+DA + G + Sbjct: 240 DEVMSGMGRTGYLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQ 299 Query: 316 LFHGYTYSGHPLACAAGLASLEVFQTEGVLDNAQAVADYFQ-EAAHALRGLPHVIDVRTI 374 HG+TY GH ACAA +A + Q E +L+N + + + E A HV D+R Sbjct: 300 --HGHTYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGR 357 Query: 375 GLIAGIEL-----ASLP--------GRVGARAYDAFVRAFNDGILIR-VTGDIIALSPPL 420 GL G+EL + LP RV A A + + G I GD I L+PP Sbjct: 358 GLFVGVELVAERSSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPF 417 Query: 421 IINKQQIDELFGKLA 435 I + I E+ +L+ Sbjct: 418 IASSNDISEIVQRLS 432 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 196 bits (499), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 135/442 (30%), Positives = 215/442 (48%), Gaps = 17/442 (3%) Query: 11 SLSAYWMPFTANRNFKAAPRMLVS-AEGMHYRDDAGNAILDGTAGLWCVPLGHAHPKIVS 69 SL + W P T R ++AP + ++ A G+ D G LD + W GH + +I + Sbjct: 13 SLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGHTNTRINA 72 Query: 70 AVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFAHVFYTGSGSESVDTALKIALAY 129 A+ + + L++A H L+E+L A TG H FY G+ +V+ ALK+++ Sbjct: 73 ALVEQLGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGASAVEIALKMSVHA 132 Query: 130 HRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNA 189 R G + I GYHG G + V R + + L P H+ + + + A Sbjct: 133 WRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPA--HVAASPDARQAA 190 Query: 190 FTKGEPEYGAHLADELERLVTLHDASNIAAVIVEP-VAGSTGVLIPPKGYLKRLREICTK 248 + E A D++ER++ IAA+IVEP V + G+ + GY++ LR +C + Sbjct: 191 PGESAEEVAARALDDVERILQ-QRQGRIAALIVEPLVQCAAGMAMHDPGYVRGLRSLCDR 249 Query: 249 HGILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKKEIHDAFM 308 +G LI DE+ G GR G+ FA + V PD+ +KG++ G +P+ V + I+ AF Sbjct: 250 YGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMSSEAIYQAFY 309 Query: 309 NGPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLDNAQAVADYFQEAAHALRGLPHV 368 + H ++Y+G+PLAC A LA+L +F + VL+ +A A A L P V Sbjct: 310 DDDVRRGFLHSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAALQPLAQDPRV 369 Query: 369 IDVRTIGLIAGIELASLPGRVGARAYDAFVR-----AFNDGILIRVTGDIIALSPPLIIN 423 VR G+I + A L G A A AF R A +L+R G + L PP +++ Sbjct: 370 RHVRQRGMIWAYD-AVLEG--SAAATTAFSRRMAAEAAQREVLVRPIGASLYLMPPYLLD 426 Query: 424 KQQI----DELFGKLAAILKTL 441 ++Q +L AA ++ L Sbjct: 427 EEQAHWLGRQLHAAFAATMEAL 448 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 171 bits (432), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 135/410 (32%), Positives = 203/410 (49%), Gaps = 37/410 (9%) Query: 42 DDAGNAILDGTAGLWCVPLGHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAY 101 D G +D AG+ + GH HPK++ A++ + + + + S ELAER+ Sbjct: 40 DVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVELAERINRL 99 Query: 102 T-GNRFAHVFYTGSGSESVDTALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGG 160 T GN + +G+E+V+ A+KIA A+ R +I G+HG GM++ G Sbjct: 100 TPGNYPKKTAFFSTGAEAVENAIKIARAH------TGRPGVIAFAGGFHGRTMMGMALTG 153 Query: 161 IGGNRKTFSTALLPG-VDHLPHTHNLEKNAFTKGEPEYGAHLADELERLVTLH----DAS 215 K PG V H P+ L +G D LE + L +A Sbjct: 154 KVAPYK-LGFGPFPGDVFHAPYPSAL-----------HGITSEDALEAVKGLFKSDIEAK 201 Query: 216 NIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHGILLIFDEVITGFGRLGTPFASDYFD 275 +AA+I+EPV G G P +++ LR +C +HGILLI DEV +G+GR G FA +++D Sbjct: 202 RVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYGRTGKLFAMEHYD 261 Query: 276 VEPDIFTTAKGLTNGVVPMGAVFVKKEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLAS 335 V PD+ T AK L G +P+ AV + EI DA P G+ G TY+G+PLA A+ LA Sbjct: 262 VLPDLMTMAKSLAGG-MPLSAVNGRAEIMDA--PAPGGL----GGTYAGNPLAIASALAV 314 Query: 336 LEVFQTEGVLDNAQAVADYFQEAAHALR-GLPHVIDVRTIGLIAGIELASLPGRVGARAY 394 L+V + E ++ Q + D QE LR +P + +VR +G + +E A Y Sbjct: 315 LDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEFADPATGKPDAEY 374 Query: 395 DAFVR--AFNDGILIRVT---GDIIALSPPLIINKQQIDELFGKLAAILK 439 V+ A N+G+L+ G++I PL I +DE G LA ++ Sbjct: 375 TKKVQQHALNNGLLLLTCGSYGNVIRFLFPLTIPDTVMDEALGILAKAIR 424 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,387,856 Number of extensions: 60639 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 10 Length of query: 442 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 354 Effective length of database: 1,203,190 Effective search space: 425929260 Effective search space used: 425929260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)