BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4499.p (406 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 563 e-162 gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 129 3e-31 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 125 8e-30 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 84 3e-17 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 61 1e-10 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 563 bits (1452), Expect = e-162, Method: Compositional matrix adjust. Identities = 305/403 (75%), Positives = 341/403 (84%), Gaps = 1/403 (0%) Query: 1 MNTSPSTPTSSTPQAQAHQ-GSLLTAGFLALGSFAIGTEGFMIAPLLPRMAADFGMSVSA 59 MN P P++ + A Q GSLLTAGFLALG+FAIGTEGFMIAPLLPRMAADFGMSV Sbjct: 1 MNPPPLGPSAPSACASPTQAGSLLTAGFLALGTFAIGTEGFMIAPLLPRMAADFGMSVPM 60 Query: 60 VASLVIVFTLVLALSSPITTVLSARINRRNLLIGAMGIFTLANLLAAHSTGFASLLSSRL 119 VASLV++FTLVLALSSPI+TVLSA +NRR +LI AM FT ANLLAA S+ F LL +RL Sbjct: 61 VASLVVIFTLVLALSSPISTVLSAAMNRRRVLIAAMTAFTAANLLAACSSSFTGLLVARL 120 Query: 120 LMAVAAGLYTPNANALAGAIVKPEQRGRALAIVSGGMTIAIALGLPLGSVVGHAFGWRAT 179 LMAVAAGLYTPNA+ALAGA+V P QRGRALAIVSGGMT ALGLPLG+VVGHAFGWRAT Sbjct: 121 LMAVAAGLYTPNASALAGAMVAPGQRGRALAIVSGGMTSPSALGLPLGAVVGHAFGWRAT 180 Query: 180 FLAVAVMGAVAVAGIWLGVDRRAGGQLHVAGLRERLGVIGQSQIRRLLAVTLFWSVGAYA 239 FLAV+ MGAVA+AGI LGV R+AG LHVAGLRER+ VIGQ Q++RLLAVTL WSVGAYA Sbjct: 181 FLAVSAMGAVALAGILLGVPRQAGAHLHVAGLRERMAVIGQPQVQRLLAVTLLWSVGAYA 240 Query: 240 AYPYIAPYLSRTLAFGEAGIGMTVSLWGAAAAVGVTLGGTLNDRFGSRRVVRAALLLLML 299 AYPYIAPYLS L+FGE+ I +TVSLWGAAAAVGV++GGTLNDR+GS RVVR +LLLLML Sbjct: 241 AYPYIAPYLSHVLSFGESRISLTVSLWGAAAAVGVSVGGTLNDRYGSARVVRVSLLLLML 300 Query: 300 AFWALAAATLLGPAQALVPVLAAVAVWGFAVWAFFPAQMARLIAAGTPAQASVALSLNTS 359 +FW LAAAT L P AL PVL AV VWGF+VWAFFPAQMARLI AG PAQA VALSLNTS Sbjct: 301 SFWTLAAATALPPPLALGPVLLAVVVWGFSVWAFFPAQMARLIGAGAPAQAPVALSLNTS 360 Query: 360 TMYLGFSLGSAVGAGILGLGVVGGIGLFAGCCAMLGLVLDRSI 402 T+YLGFSLGSA GAGILG G + GIGLFAG CA++ +L+R + Sbjct: 361 TLYLGFSLGSAAGAGILGSGAIWGIGLFAGACALVAWLLERRL 403 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 114/367 (31%), Positives = 193/367 (52%), Gaps = 25/367 (6%) Query: 28 LALGSFAIGTEGFMIAPLLPRMAADFGMSVSAVASLVIVFTLVLALSSPITTVLSARINR 87 L + +FAIGT F+I LLP +AA+ G+S+S+ LV + L + + +P+ T + R+ R Sbjct: 8 LTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGRLPR 67 Query: 88 RNLLIGAMGIFTLANLLAAHSTGFASLLSSRLLMAVAAGLYTPNANALAGAIVKPEQRGR 147 + LL+ M +F + NL A + + +L+ R++ + A G + + +A ++V E++ Sbjct: 68 KTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQAS 127 Query: 148 ALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFLAVAVMGAVAVAGIWLGVDRRAGGQLH 207 A+A++ G+T+A LG+P G+ +G ++GWRATF AV V+G +A+A + V R GG+ Sbjct: 128 AIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGGEET 187 Query: 208 VAGLRERLGVIGQSQIRRLLAVTLFWSVGAYAAYPYIAPYLSRTLAFGEAGIGMTVSLWG 267 G R V+ + Q+ L +T+ G +AA+ YIAP L+ F + + + L+G Sbjct: 188 DFGKEVR--VLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLFG 245 Query: 268 AAAAVGVTLGGTLNDR------FGSRRVVRAALLLLMLAFWALAAATLLGPAQALVPVLA 321 +G GG L D+ GS ++ A L+L L AA + Sbjct: 246 LGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAII------------ 293 Query: 322 AVAVWGFAVWAFFPAQMARLIAAGTPAQASVALSLNTSTMYLGFSLGSAVGAGILGLGVV 381 V + G A +A P R++ +A+ A +L +S F+LG+A GA + G+ + Sbjct: 294 GVGLMGMAAFATVPPLQMRVL-----EKATGAPNLASSANIAAFNLGNAAGAWLGGVTID 348 Query: 382 GGIGLFA 388 G GL A Sbjct: 349 HGPGLHA 355 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 125 bits (313), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 110/365 (30%), Positives = 182/365 (49%), Gaps = 25/365 (6%) Query: 28 LALGSFAIGTEGFMIAPLLPRMAADFGMSVSAVASLVIVFTLVLALSSPITTVLSARINR 87 LA+ +F IGT F+I LLP +A D G++ A LV + L +A +PI +L+A+ R Sbjct: 8 LAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAILTAKWPR 67 Query: 88 RNLLIGAMGIFTLANLLAAHSTGFASLLSSRLLMAVAAGLYTPNANALAGAIVKPEQRGR 147 + L+G M +F L N+L A + + L+ +R++ A + + +A +V P++R + Sbjct: 68 KQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVAPQKRAQ 127 Query: 148 ALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFLAVAVMGAVAVAGIWLGVDRRAGGQLH 207 A+A++ G+T+A LG+PLG+ GHA GWR+TF AV +G +A +W + ++ + Sbjct: 128 AVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKKI--PMQ 185 Query: 208 VAGLRERLGVIGQSQIRRLLAVTLFWSVGAYAAYPYIAPYLSRTLAFGEAGIGMTVSLWG 267 + + I ++++ L + S+ ++ YIA L F + L+G Sbjct: 186 AGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWVLLLFG 245 Query: 268 AAAAVGVTLGGTLNDRFGSRRVVRAALLLLMLAFWALAAATLLG----PAQALVPVLAAV 323 A VG +GG L D L+ +M + L A +LG + P LA + Sbjct: 246 AGITVGGLVGGKLADW---------KLMPVMCGVF-LCIAVVLGLFSITSHYKWPTLATM 295 Query: 324 AVWGFAVWAFFPAQMARLI--AAGTPAQASVALSLNTSTMYLGFSLGSAVGAGILGLGVV 381 WG +A P R++ A G P AS +LN F+LG+A GA + GL + Sbjct: 296 FAWGAFAFAVVPGAQIRVLDTARGAPNLAS---TLNQG----AFNLGNATGAWVGGLAIS 348 Query: 382 GGIGL 386 G+ L Sbjct: 349 AGLPL 353 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 83.6 bits (205), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 65/223 (29%), Positives = 119/223 (53%), Gaps = 2/223 (0%) Query: 28 LALGSFAIGTEGFMIAPLLPRMAADFGMSVSAVASLVIVFTLVLALSSPITTVLSARINR 87 LALG F++ T F+ A LL +A+D +S A V +V A+++P +L+ I+R Sbjct: 42 LALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNIDR 101 Query: 88 RNLLIGAMGIFTLANLLAAHSTGFASLLSSRLLMAVAAGLYTPNANALAGAIVKPEQRGR 147 + +++ + ++N+LA + LL +R+L+ VA G + A ALA +V R Sbjct: 102 KRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFAR 161 Query: 148 ALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFLAVAVMGAVAVAGIWLGVDRRAGGQLH 207 A++++ G+++A P+G+ +G+ +GWR+ F+A V+ VA+A W+ + R H Sbjct: 162 AMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKD-H 220 Query: 208 VAGLRERLGVIGQSQIRRLLAVTLFWSVGAYAAYPYIAPYLSR 250 L+ ++G+ +R L L G +A + YI P + + Sbjct: 221 -PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQ 262 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 53/194 (27%), Positives = 88/194 (45%), Gaps = 15/194 (7%) Query: 1 MNTSPSTPTSSTPQAQAHQGSLLTAGFLALGSFAIGTEGFMIAPL----LPRMA---ADF 53 MN P+TP+ S P + G LA FA+ + +++PL +P M F Sbjct: 1 MN-HPATPSLSPPVQGFTPYQKIVTGLLAFLQFAVILDFMLMSPLGALIMPSMQITPQQF 59 Query: 54 GMSVSAVASLVIVFTLVLALSSPITTVLSARINRRNLLIGAMGIFTLANLLAAHSTGFAS 113 G+ VSA A S +T + R +R+ LL+ G F L + + F S Sbjct: 60 GLVVSAYA-------FSAGASGLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSFES 112 Query: 114 LLSSRLLMAVAAGLYTPNANALAGAIVKPEQRGRALAIVSGGMTIAIALGLPLGSVVGHA 173 LL +R++ + G+ A+A + P RGR + ++ + LG+P+G + + Sbjct: 113 LLLARIVTGLFGGVIGSITLAIATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLSNR 172 Query: 174 FGWRATFLAVAVMG 187 + W FLA+A +G Sbjct: 173 WNWHVPFLAMAALG 186 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 894,936 Number of extensions: 31355 Number of successful extensions: 368 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 365 Number of HSP's successfully gapped: 18 Length of query: 406 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 319 Effective length of database: 1,207,925 Effective search space: 385328075 Effective search space used: 385328075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)