BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4498.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum ... 442 e-125 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 84 2e-17 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 82 4e-17 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 76 3e-15 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 75 4e-15 >gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 442 bits (1137), Expect = e-125, Method: Compositional matrix adjust. Identities = 216/252 (85%), Positives = 233/252 (92%) Query: 1 MKLTDNTLFITGGTSGIGRALAEAFHKLGNQVIIGGRRKALLDEVTAANPGMDAIEFDVS 60 M+LTDNT+FITGG SGIGR LAEAFHKLGN VIIGGRR+ALLDEV AANPGM AI FDV+ Sbjct: 1 MQLTDNTIFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPGMQAIAFDVA 60 Query: 61 SLASIDAAAATLKARYPKLNVLINNAGIMPFDDAGSTIDDQVSRNILDTNLLGPIRLTSA 120 ASIDAAAATLKARYP LNVL+NNAGIMPFDDA IDD+V+R ILDTNLLGPIRLTSA Sbjct: 61 DPASIDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSA 120 Query: 121 LIELLKAQPSATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTHVRVQEIA 180 LI+LLK QP ATI+HNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLR T VRVQEIA Sbjct: 121 LIDLLKQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEIA 180 Query: 181 PPWVDTDLVKKSGDPRAMPLEAFIAETMSKLASDDEEIIVEAIKPLRDNPGAKEHALVNG 240 PPWVDTDLVKKSGDPRAMPL+AFI ETM++LAS+DEEIIV+A+KPLRDNPGAKEHALVNG Sbjct: 181 PPWVDTDLVKKSGDPRAMPLDAFITETMARLASEDEEIIVDAVKPLRDNPGAKEHALVNG 240 Query: 241 FNEALVANPIPV 252 FN AL+A+PIPV Sbjct: 241 FNAALMAHPIPV 252 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 69/214 (32%), Positives = 107/214 (50%), Gaps = 10/214 (4%) Query: 8 LFITGGTSGIGRALAEAFHKLGNQVIIGGRRKALLDEVTAANPGMDAI---EFDVSSLAS 64 + ITG T+G G A+A F G++VI GRRK LD++ G D + DV+S +S Sbjct: 3 VLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVK-ELGADKVLPLVLDVTSKSS 61 Query: 65 IDAAAATLKARYPKLNVLINNAGIMPFDDAGSTIDDQVSRNILDTNLLGPIRLTSALIEL 124 I+ A+L + +++VLINNAG+ + ++DTN+ G + +T AL+ Sbjct: 62 IETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLPD 121 Query: 125 LKAQPSATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTHVRVQEIAPPWV 184 + + TII+ SV P VY A+KA + + ++ R L T VR +AP Sbjct: 122 MVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGLC 181 Query: 185 -DTDL--VKKSGDPRAMPLEAFIAETMSKLASDD 215 T+ V+ GD A A + E + L +DD Sbjct: 182 GGTEFSNVRFKGDDGA---AAKVYEGTTPLTADD 212 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 82.0 bits (201), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 63/202 (31%), Positives = 101/202 (50%), Gaps = 12/202 (5%) Query: 3 LTDNTLFITGGTSGIGRALAEAFHKLGNQVIIGGRRKALLDEVTAANPGMDAIEFDVSSL 62 L T +TGGT GIG A+A LG +VI G +E + + +E +V+S Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEEFAGSE--VTLVEMNVASE 65 Query: 63 ASIDAAAATLKARYPKLNVLINNAGIMPFDDAGSTIDDQVSRNILDTNLLGPIRLTSALI 122 S+ AA A L +L++++N AGI+ G+ ++ +V ++ NL G +R+ +A Sbjct: 66 ESVRAALAGLT----QLDIVVNCAGII---KRGAELETEVFEQVIAVNLTGTMRVCAAAR 118 Query: 123 ELLKAQPSATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTHVRVQEIAPP 182 ELL+ Q I++ S+L++ + YSASK + S +RV +AP Sbjct: 119 ELLR-QSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 Query: 183 WVDTDLVKK-SGDP-RAMPLEA 202 W+ T L + DP RA P+ A Sbjct: 178 WIATPLTQALQDDPVRAGPILA 199 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 75.9 bits (185), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 64/193 (33%), Positives = 95/193 (49%), Gaps = 18/193 (9%) Query: 2 KLTDNTLFITGGTSGIGRALAEAFHKLGNQVIIGGRRKALLD-EVTAANPGMDAIEFDVS 60 +L +TGGTSGIG A A+ G QV I GRR+A LD V + I D S Sbjct: 4 QLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRADAS 63 Query: 61 SLASIDAAAATLKARYPKLNVLINNAG---IMPFDDAGSTIDDQVSRNILDTNLLGPIRL 117 L+ +D A + +L++L NAG +MP G+ ++ R I TN+ G Sbjct: 64 VLSELDTVYAQIAKSAGRLDILFANAGGGDMMPL---GAITEEHFDR-IFGTNVRG---- 115 Query: 118 TSALIELLKAQP----SATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTH 173 L + KA P A++I +S + + A +VYSASKAA+ ++A S L+ Sbjct: 116 --VLFTVQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRA 173 Query: 174 VRVQEIAPPWVDT 186 +RV ++P + T Sbjct: 174 IRVNVVSPGPIRT 186 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 75.5 bits (184), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 60/197 (30%), Positives = 99/197 (50%), Gaps = 7/197 (3%) Query: 3 LTDNTLFITGGTSGIGRALAEAFHKLGNQVIIGGRR----KALLDEVTAANPGMDAIEFD 58 + D + ITG +SG+G A LG ++++G RR + L+ ++TAA A+ D Sbjct: 5 IKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVTTD 64 Query: 59 VSSLASIDAAAATLKARYPKLNVLINNAGIMPFDDAGSTIDDQVSRNILDTNLLGPIRLT 118 V+ ++A A + + +++VL+NNAGIMP ++ R ++D N+ G + Sbjct: 65 VARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDR-MIDVNVKGVLYGV 123 Query: 119 SALIELLKAQPSATIIHNTSVLAYVPLA-VTAVYSASKAALHSYAMSQRFMLRHTHVRVQ 177 +A + AQ S II+ +SV A + VYSA+K A+ + R VR Sbjct: 124 AAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEA-PDGVRTT 182 Query: 178 EIAPPWVDTDLVKKSGD 194 I+P VD++L S D Sbjct: 183 IISPGAVDSELKTHSSD 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 761,449 Number of extensions: 29680 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 29 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)