BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4497.p (269 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herb... 455 e-129 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 91 1e-19 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 88 9e-19 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 82 4e-17 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 82 4e-17 >gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 270 Score = 455 bits (1171), Expect = e-129, Method: Compositional matrix adjust. Identities = 227/270 (84%), Positives = 243/270 (90%), Gaps = 1/270 (0%) Query: 1 MNQT-TTTQRGTALITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRR 59 MNQ T RGTAL+TGASSGIGA+YADRLAR GYDLILVARNR RLN LAERI+N T R Sbjct: 1 MNQPHTPAHRGTALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGR 60 Query: 60 TVEVLSADLGNAQSLAQVEDKLRRDASITLLVNNAGIGTHTPLLQSDVEHMTDMIALNVT 119 +VEVL ADLG A SLAQVE+KLR DASITLLVNNAGIGTHTPLLQSDVEHMT MIALNVT Sbjct: 61 SVEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSDVEHMTGMIALNVT 120 Query: 120 APTRLTYAAVPGFVARGRGAIINISSIVGVAPEILNGVYGGSKAFVLAFSQSLHHELADQ 179 APTRLTYAAVPGFVARG+GA+INISSIVGVAPE+LNGVYGGSKAFVLAFSQSLHHELA Q Sbjct: 121 APTRLTYAAVPGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQ 180 Query: 180 GVQVQAVLPGATATDFWAIGGLPLEHLDPAIVMPAEQMVDAALVGFERGELVTIPSLHDE 239 GVQVQAVLPGATATDFWAIGGLP+EHLDPAIVM AEQMVDAALVGF+R ELVTIPSLH+E Sbjct: 181 GVQVQAVLPGATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQRRELVTIPSLHEE 240 Query: 240 DAWIRFDSARQSLSTQLSANVPATRYAVTH 269 DAW +++ARQ+L +LSAN PA RY H Sbjct: 241 DAWTGYEAARQALIARLSANTPAPRYRAAH 270 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 90.5 bits (223), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 70/195 (35%), Positives = 100/195 (51%), Gaps = 24/195 (12%) Query: 13 LITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVLSADLGNAQ 72 LITGA++G GA A + A G+ +I R +ERL+ L + + D +VL L + Sbjct: 4 LITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGAD-----KVLPLVL-DVT 57 Query: 73 SLAQVEDKLRRDASIT-------LLVNNAGI----GTHTPLLQSDVEHMTDMIALNVTAP 121 S + +E L AS+T +L+NNAG+ G + SD E M D NV Sbjct: 58 SKSSIETGL---ASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDT---NVKGL 111 Query: 122 TRLTYAAVPGFVARGRGAIINISSIVGVAPEILNGVYGGSKAFVLAFSQSLHHELADQGV 181 +T+A +P V RGRG IIN+ S+ G P VYG +KAFV F+ +L +L GV Sbjct: 112 VTITHALLPDMVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGV 171 Query: 182 QVQAVLPG-ATATDF 195 + + PG T+F Sbjct: 172 RATNLAPGLCGGTEF 186 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 87.8 bits (216), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 68/200 (34%), Positives = 101/200 (50%), Gaps = 7/200 (3%) Query: 6 TTQRGTALITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVLS 65 T Q ALITGAS+GIG A AR G LIL AR ++ L +A I R V++ Sbjct: 3 TLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRA-SVVA 61 Query: 66 ADLGNAQSLAQ-VEDKLRRDASITLLVNNAG-IGTHTPLLQSDVEHMTDMIALNVTAPTR 123 D+G A + + V L + + +NNAG +G PL + + D + N+T+ Sbjct: 62 GDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFL 121 Query: 124 LTYAAVPGFVARGRGAIINISSIVGVAPEILN-GVYGGSKAFVLAFSQSLHHELADQGVQ 182 +P +ARG GAI+ SS VG + + YG +KA ++ + + + A QG++ Sbjct: 122 GARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIR 181 Query: 183 VQAVLPGATATDFWAIGGLP 202 A+LPG TD +GG P Sbjct: 182 ANALLPGGVDTD---MGGDP 198 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 82.4 bits (202), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 63/187 (33%), Positives = 99/187 (52%), Gaps = 5/187 (2%) Query: 12 ALITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVLSADLGNA 71 AL+TGAS GIG A LARQG +I A + A+ E ++ ++ +A Sbjct: 11 ALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVLNVNDA 70 Query: 72 QSLAQVEDKLRRD-ASITLLVNNAGIGTHTPLLQSDVEHMTDMIALNVTAPTRLTYAAVP 130 ++ + +++++D S+++LVNNAGI ++ E +IA N+TA RL+ A + Sbjct: 71 EASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAVLR 130 Query: 131 GFVARGRGAIINISSIVGVA--PEILNGVYGGSKAFVLAFSQSLHHELADQGVQVQAVLP 188 G + G IINI+S+VG A P +N Y +KA V S++L E+ + + V V P Sbjct: 131 GMMKARTGRIINITSVVGEAGNPGQMN--YAAAKAGVAGMSRALAREIGSRNITVNCVAP 188 Query: 189 GATATDF 195 G TD Sbjct: 189 GFIDTDM 195 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 82.0 bits (201), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 68/199 (34%), Positives = 92/199 (46%), Gaps = 32/199 (16%) Query: 13 LITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVLSADLGNAQ 72 +ITGASSG+G A LA G L+L AR ERL L I+ Q Sbjct: 11 VITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADIT-------------AAGGQ 57 Query: 73 SLAQVEDKLRRD-------------ASITLLVNNAGIGTHTPLLQSDVEHMTDMIALNVT 119 ++A D RRD I +LVNNAGI P+ + VE MI +NV Sbjct: 58 AIAVTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVK 117 Query: 120 APTRLTYAAVPGFVARGRGAIINISSIVGVAPEILNG---VYGGSKAFVLAFSQSLHHEL 176 AA+P F A+ G IIN+SS+ G+ ++ G VY +K V A ++ L E Sbjct: 118 GVLYGVAAALPRFAAQSSGHIINVSSVAGI--KVFAGMGTVYSATKFAVRALTEGLRTEA 175 Query: 177 ADQGVQVQAVLPGATATDF 195 D GV+ + PGA ++ Sbjct: 176 PD-GVRTTIISPGAVDSEL 193 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 836,695 Number of extensions: 36176 Number of successful extensions: 259 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 44 Length of query: 269 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 185 Effective length of database: 1,222,130 Effective search space: 226094050 Effective search space used: 226094050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)