BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4484.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 676 0.0 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 109 3e-25 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 97 3e-21 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 96 4e-21 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 92 6e-20 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 676 bits (1743), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/406 (90%), Positives = 392/406 (96%), Gaps = 2/406 (0%) Query: 1 MSHDRHSELGIHPLAEGEPQQRQQMMRRARLVLVVVLVLLAAGAARTVINRVANARALEA 60 MSHDRHSELGIHPL+EGEPQQRQQMMRRAR+VLVVVL+LLAAGAARTVI+RVANAR L+A Sbjct: 1 MSHDRHSELGIHPLSEGEPQQRQQMMRRARVVLVVVLLLLAAGAARTVISRVANARELQA 60 Query: 61 GTAERAKVFVRVAPARSSADGQTLALPGTLQGFVQSPISARSGGYLKRWYKDIGAEVKKG 120 GTAERAK+FVRVAP RSSA+GQTLALPGTLQGFVQSPISARSGGYLKRW KDIGAEVKKG Sbjct: 61 GTAERAKMFVRVAPVRSSAEGQTLALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKG 120 Query: 121 DLLAELDTPEIDQQLSQALATRQQAASSMELARSSMARWEALRKKDAVSQQELDERRSAF 180 +LLAELDTPEIDQQL+QALA R+QAASSMELARSSMARWEALRKKDAVSQQELDERRSAF Sbjct: 121 ELLAELDTPEIDQQLTQALAAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAF 180 Query: 181 AQASSNLAAADANVERLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRSLFVLSQTD 240 AQAS+NLAAADANV RLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGR+LFVLSQTD Sbjct: 181 AQASANLAAADANVGRLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTD 240 Query: 241 PLRVYVNVPQSYANLVKPGQQVTVTQAELRGRKFEGKVARTAGSIDPVTRSMQVEVTLSN 300 PLRVYVNVPQSYANLVKPGQQVTVTQ+ELRGRKFEGKVARTAG+ID TRSMQVEVTLSN Sbjct: 241 PLRVYVNVPQSYANLVKPGQQVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSN 300 Query: 301 ADNALLPGAFVQVALPLKPSGTLLVAADTLMFRRDGSLVAAVDEHNRVRLKKVRLGRNFG 360 AD+ALLPGAFVQVALP+KPSG LLVAADTLMFRRDG+LVAAVD HN+VRLKK+RLGRNFG Sbjct: 301 ADHALLPGAFVQVALPIKPSGALLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFG 360 Query: 361 LTVEVLEGLNGNEKLILNPSDSLAEGDQVEPTLEQPE--QKQKAAS 404 TVEVL+GL G+E LILNPSD+LAEGDQVEPTLEQP+ +KQKAAS Sbjct: 361 QTVEVLDGLQGDEHLILNPSDALAEGDQVEPTLEQPKEPEKQKAAS 406 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 101/345 (29%), Positives = 164/345 (47%), Gaps = 23/345 (6%) Query: 69 FVRVAPARSSADGQTLALPGTLQGFVQSPISARSGGYL-KRWYKDIGAEVKKGDLLAELD 127 V +AP R + + LPG ++ F + + AR G + KR +K+ G+EVK+G +L ++D Sbjct: 41 VVTIAPQRVAITNE---LPGRVEAFRVAQVRARVAGIVQKRVFKE-GSEVKEGQVLFKID 96 Query: 128 TPEIDQQLSQALATRQQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASSNL 187 + L A AT +A +++ + R++ L + +AVS+QE D +A QA +++ Sbjct: 97 PAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADV 156 Query: 188 AAADANVERLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRSLFVLSQTDPLRVYVN 247 AA A V+ G+ + +P SG I R V G L+ G L ++ Q DP VYVN Sbjct: 157 AAGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDP--VYVN 214 Query: 248 VPQSYANLVKPGQQVTVTQAELRGRK---------------FEGKVARTAGSIDPVTRSM 292 V QS A+L+K Q + Q + G GK+ + S+D T S+ Sbjct: 215 VTQSSADLLKLQQAMAAGQLKSVGDNKASVTLELENGQAYPLPGKLLFSDISVDENTGSV 274 Query: 293 QVEVTLSNADNALLPGAFVQVALPLK-PSGTLLVAADTLMFRRDGSLVAAVDEHNRVRLK 351 + N LLPG +V+ + LLV ++ GS V V+ N+V + Sbjct: 275 SLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPR 334 Query: 352 KVRLGRNFGLTVEVLEGLNGNEKLILNPSDSLAEGDQVEPTLEQP 396 V+ G G +VL+GL +++I+ + G V+P P Sbjct: 335 PVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATVKPVQWNP 379 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 96.7 bits (239), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 78/303 (25%), Positives = 142/303 (46%), Gaps = 13/303 (4%) Query: 98 ISARSGGYLKRWYKDIGAEVKKGDLLAELDTPEIDQQLSQALATRQQAASSMELARSSMA 157 + AR G L + + G VK GDLLAE+D + L+QA + + + AR + Sbjct: 62 VRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDLK 121 Query: 158 RWEALRKKDAVSQQELDERRSAFAQASSNLAAADANVERLRQVEGFKRIVAPFSGIITRR 217 R++ L +D+++ Q++D + + Q + A + NV R F R+ AP SG + R Sbjct: 122 RYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGLR 181 Query: 218 NVDVGDLIDPGAGRSLFVLSQTDPLRVYVNVPQSYANLVKPGQQVTVTQA--------EL 269 DVG+ + L +++Q P+ ++P+ N+ K QQ+ + E Sbjct: 182 QADVGNNVTTSDTNGLVIITQLQPITAIFSIPED--NIPKVLQQLQSGRKLPAQAWDREQ 239 Query: 270 RGRKFEGKVARTAGSIDPVTRSMQVEVTLSNADNALLPGAFVQVALPLKPS-GTLLVAAD 328 + + +G + +D T +++++ N D AL P FV + L L G ++ Sbjct: 240 KNKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTA 299 Query: 329 TLMFRRDGSLVAAVDEHNRVRLKKVRLGR-NFGLTVEVLEGLNGNEKLILNPSDSLAEGD 387 + G V V + + V ++ V+ G LTV + +G++ E ++++ D L EG Sbjct: 300 AIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTV-ITDGVSAGETVVIDGIDRLREGA 358 Query: 388 QVE 390 +VE Sbjct: 359 KVE 361 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 96.3 bits (238), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 78/284 (27%), Positives = 140/284 (49%), Gaps = 8/284 (2%) Query: 82 QTLALPGTLQGFVQSPISARSGGYLKRWYKDIGAEVKKGDLLAELDTPEIDQQLSQALAT 141 Q LA GT+ + R G L++ G +VKKG LLA++D + QL+QA A Sbjct: 63 QYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAEAQ 122 Query: 142 RQQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASSNLAAADANVERLRQVE 201 R + ++++ AR + R+ LR +DA +QQ+LD +++ AQ + + +A V + Sbjct: 123 RAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKVQL 182 Query: 202 GFKRIVAPFSGIITRRNVDVGDLIDPGAGRSLFVLSQTDPLRVYVNVPQSYANLVKPGQ- 260 + I AP SG + R VD G+++ L V++Q DP+ + +P+ + Q Sbjct: 183 SYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRAQR 242 Query: 261 ------QVTVTQAELRGRKFEGKVARTAGSIDPVTRSMQVEVTLSNADNALLPGAFVQVA 314 QV+ + + GK+ I+ ++Q++ N D+ L PG +V Sbjct: 243 AHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNAR 302 Query: 315 LPLKPSGT-LLVAADTLMFRRDGSLVAAVDEHNRVRLKKVRLGR 357 L ++ G L V A + + G+ V A+D+ ++ R++ V++ R Sbjct: 303 LQMRSHGNALTVPAAAIQRNQQGTYVYAIDQEDQARMQPVKVER 346 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 92.4 bits (228), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 107/395 (27%), Positives = 178/395 (45%), Gaps = 32/395 (8%) Query: 11 IHPLAEGEPQQRQQMMRRARLVLVVVLVLLAAGAARTVINRVANARALEAGTAERAKVFV 70 +HP A +Q + ARL L ++LV + A +R+ A+A E G +V Sbjct: 1 MHPTASTPRRQGRTSSATARLALSLILVAVLAACSRSGDQPKPTAQA-EVG-------YV 52 Query: 71 RVAPARSSADGQTLALPGTLQGFVQSPISARSGGYL-KRWYKDIGAEVKKGDLLAELDTP 129 V R + T LPG ++ + I + GG + KR +K+ G+ VK G L ++D Sbjct: 53 VVKAQRQAL---TTELPGRTTAYLAADIRPQVGGIIQKRLFKE-GSTVKAGQPLYQIDPA 108 Query: 130 EIDQQLSQALATRQQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASSNLAA 189 A A+ +A +++ ++ R+ L K DAVS+Q+ D+ +S Q +++ Sbjct: 109 SYQAAYDSARASLLKAEATLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEV 168 Query: 190 ADANVERLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRSLFVLSQTDPLRVYVNVP 249 A V+ + +I++P SG I + V G L+ +L + Q DP +YV+V Sbjct: 169 QKAAVQTAAINLQYTKILSPISGRIEKSAVTPGALVTASQTTALTTVQQIDP--IYVDVT 226 Query: 250 QSYANLV------------KPGQQVTVTQAELR-GRKFE--GKVARTAGSIDPVTRSMQV 294 QS +L+ K G+ + L G +E GK+ T S+D T + + Sbjct: 227 QSTVDLLRLRRELASGQIRKSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITL 286 Query: 295 EVTLSNADNALLPGAFVQVALPLKPSGTLLVAADTLMFR-RDGSLVAAV-DEHNRVRLKK 352 + N D LLPGA+V+ L L+A + R + G+ A V +V+ + Sbjct: 287 RAVVPNPDGVLLPGAYVRAVLEEGVDDHALLAPQKAVSRDQTGAATALVLGADGKVQQRL 346 Query: 353 VRLGRNFGLTVEVLEGLNGNEKLILNPSDSLAEGD 387 V + R G V GL +KLI++ + GD Sbjct: 347 VSVARAVGNNWWVSRGLKEGDKLIVDGLQKVRTGD 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,081,909 Number of extensions: 40900 Number of successful extensions: 213 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 11 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)