BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4483.p (501 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 767 0.0 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 284 1e-77 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 280 2e-76 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 269 5e-73 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 222 5e-59 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 767 bits (1980), Expect = 0.0, Method: Compositional matrix adjust. Identities = 392/455 (86%), Positives = 429/455 (94%) Query: 20 GCASGPDYVKPAVELPVAWTPEAPWRVMQPQDASPRGPWWERFHDARLNALQQQALAGNQ 79 GCASGPDY +P V++P AWT EAPWR MQP DA+PRGPWWERF DA+LNALQQQALAGNQ Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQ 77 Query: 80 TLAIASARLAQARAQLEVVGAAQSPQVGINTRAARARISANRPLTNYNSPNFSTVQNDFA 139 TLAIA+ARLAQARAQL V GAAQ+PQV +N RAAR+RISANRPLTNYNSPNFSTVQNDFA Sbjct: 78 TLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNYNSPNFSTVQNDFA 137 Query: 140 LGLNVSYELDFFGRVRRSVEAASASAQQSAADLENTRLLLTAELASNYFNLRELDIELDV 199 LG +VSYE+DFFGRV+RSVEAA A+A+QSAADLENTRLLLTAELA+NYFNLRELD+ELDV Sbjct: 138 LGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDV 197 Query: 200 VQRAIALQRKALELATSRHDLGATSGLDVAQQQALLDNTLTQVDILGRQRAQYEHAIATL 259 V+RAIALQR+ALELAT+RHDLGATSGLD+AQQQALLD+TLTQ+D+LGRQRAQYEHAIATL Sbjct: 198 VRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATL 257 Query: 260 TGTPAPGFTIAPSLAPIALPAIPLGVPSDVLERRPDIASAERAMAAANAQIGVASAAFYP 319 TGTPAP F+I PSL PI PAIP+GVPSDVLERRPDIA+AERAMAAANAQIGVASAA+YP Sbjct: 258 TGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYP 317 Query: 320 SFMLQPGYGVDSRNWGALFNAPSVLWSLGVSASQSLFDAGRLRAGVDFSKAGYDATVATY 379 SFMLQP YGVDSRNW LFNAPS+LWSLGVSASQSLFD GRLRAGVDFS+AGY+ATVA+Y Sbjct: 318 SFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASY 377 Query: 380 RRTVLTAMQEVEDGITGIAALDRAYGQSQSAIASARRVLDIANSRYEGGVTPYLDVITAQ 439 RRTVLTAMQEVEDGITG+AAL+RAY QSQ AIASARRVL+IA+SRYEGG TPYLDVITAQ Sbjct: 378 RRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQ 437 Query: 440 QALLNSERQSAQLMGQRLLVSVFLIKALGGDWQER 474 Q+LLNSERQ+AQLMGQRLLVSVFLIKALGGDWQ R Sbjct: 438 QSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSR 472 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 284 bits (726), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 198/489 (40%), Positives = 282/489 (57%), Gaps = 26/489 (5%) Query: 3 LRSLLAASTVLTALMLAGCASGPDYVKPAVELPVAWTPEAPWRVMQPQDASPRGPWWERF 62 R+ + ++ A +L GCA GPDY +PA++LP A+ PW++ P+ PWWE + Sbjct: 29 FRATKGMTALVLAALLGGCAVGPDYARPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAY 88 Query: 63 HDARLNALQQQALAGNQTLAIASARLAQARAQLEVVGAAQSPQVGINTRAARARISANRP 122 D LNAL QA A NQT+ A+A+ QA+A EV A+ P +G A+RA+ + N Sbjct: 89 GDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTN-- 146 Query: 123 LTNYNSPNFSTVQNDFALGLNVSYELDFFGRVRRSVEAASASAQQSAADLENTRLLLTAE 182 + N +++GLN S+E D +GR+RR EA A+AQ SAA L RL + A Sbjct: 147 -------GVQRLGNSYSVGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQAT 199 Query: 183 LASNYFNLRELDIELDVVQRAIALQRKALELATSRHDLGATSGLDVAQQQALLDNTLTQV 242 LA +Y LR D++ D+ +R +A ++L+L+T +++ G DVAQ + L Q+ Sbjct: 200 LAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQL 259 Query: 243 DILGRQRAQYEHAIATLTGTPAPGFTIAPSLAPI----------------ALPAIPLGVP 286 L R Q EHAIA L G F++ P+L P +LP IP+G+P Sbjct: 260 IDLDDTRNQLEHAIAMLIGKAPAQFSL-PALPPAAASDSAIDANAARLQGSLPQIPVGLP 318 Query: 287 SDVLERRPDIASAERAMAAANAQIGVASAAFYPSFMLQPGYGVDSRNWGALFNAPSVLWS 346 S++LERRPDIA+AER AAANA IGVA AA++P+ L G +S + LFN PS +WS Sbjct: 319 SELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWS 378 Query: 347 LGVSASQSLFDAGRLRAGVDFSKAGYDATVATYRRTVLTAMQEVEDGITGIAALDRAYGQ 406 LG + ++SLFD G A D + A YDA VA Y++TVL +QEVED ++ + LD+ Sbjct: 379 LGSALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTV 438 Query: 407 SQSAIASARRVLDIANSRYEGGVTPYLDVITAQQALLNSERQSAQLMGQRLLVSVFLIKA 466 A+ SA+ +A +YE G YL V+T Q A L ++R + L+G++L+ SV LIKA Sbjct: 439 QAQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKA 498 Query: 467 LGGDWQERR 475 GG WQ R Sbjct: 499 TGGGWQAGR 507 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 280 bits (716), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 188/478 (39%), Positives = 275/478 (57%), Gaps = 9/478 (1%) Query: 2 RLRSLLAASTVLTALMLAGCASGPDYVKPAVEL-PVAWTPEAPWRVMQPQDASPRGPWWE 60 +L+ LLA ++ +L LA C+ GPDYV+P ++ P + W+ QP+D G WWE Sbjct: 8 KLKPLLA--MLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVADGKWWE 65 Query: 61 RFHDARLNALQQQALAGNQTLAIASARLAQARAQLEVVGAAQSPQVGINTRAARARISAN 120 F D +LNAL Q NQ LA+A A A A ++ +A SP + + A+R + Sbjct: 66 MFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSGSA 125 Query: 121 RPLTNYNSPNFST--VQNDFALGLNVSYELDFFGRVRRSVEAASASAQQSAADLENTRLL 178 ++ + S V+N + L ++E D +G+VRR VE++ ASAQ SAADL RL Sbjct: 126 GASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAARLS 185 Query: 179 LTAELASNYFNLRELDIELDVVQRAIALQRKALELATSRHDLGATSGLDVAQQQALLDNT 238 A++A NY LR LD + +++ + ++L++A +++ G + DV Q Q L + Sbjct: 186 AQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSA 245 Query: 239 LTQVDILGRQRAQYEHAIATLTGTPAPGFTI--APSLAPIALPAIPLGVPSDVLERRPDI 296 G RAQ EHAIA L G F + AP LA +A P +P GVPS +LERRPD+ Sbjct: 246 QASALDNGVTRAQVEHAIALLVGKTPAQFALPRAP-LAAVA-PPVPQGVPSMLLERRPDV 303 Query: 297 ASAERAMAAANAQIGVASAAFYPSFMLQPGYGVDSRNWGALFNAPSVLWSLGVSASQSLF 356 A+AER +A+ANA+IGVA AA++PS L G S + F P +WSLG +Q++F Sbjct: 304 AAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQTIF 363 Query: 357 DAGRLRAGVDFSKAGYDATVATYRRTVLTAMQEVEDGITGIAALDRAYGQSQSAIASARR 416 D G A D + A YD TVATY++TVLTA QEVED + + L++ A+ +AR+ Sbjct: 364 DGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAARQ 423 Query: 417 VLDIANSRYEGGVTPYLDVITAQQALLNSERQSAQLMGQRLLVSVFLIKALGGDWQER 474 + + N++Y+ G Y++VITAQ L++E S ++ +R+ SV L KALGG W ER Sbjct: 424 AVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDER 481 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 269 bits (687), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 187/467 (40%), Positives = 262/467 (56%), Gaps = 18/467 (3%) Query: 20 GCASGPDYVKPAVELPVAWTPEAP----------WRVMQPQDASPRGPWWERFHDARLNA 69 GC+ P Y +P+V+ P A+ AP W+ QP + RG WW+ F D +LNA Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 70 LQQQALAGNQTLAIASARLAQARAQLEVVGAAQSPQVGINTRAARARISANRPLTNYNSP 129 L+ A NQ L A+ARL QARA A PQV A R R S P + Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPS---PASQGLPA 146 Query: 130 NFSTVQND-FALGLNVSYELDFFGRVRRSVEAASASAQQSAADLENTRLLLTAELASNYF 188 + T N + +NVSYE+D FGRV +V+AA+A AQ+S A + L L A++A YF Sbjct: 147 DADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYF 206 Query: 189 NLRELDIELDVVQRAIALQRKALELATSRHDLGATSGLDVAQQQALLDNTLTQVDILGRQ 248 +RELD + + + L++++L L R D G S +DVA+ +A L + ++ + RQ Sbjct: 207 LVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIARQ 266 Query: 249 RAQYEHAIATLTGTPAPGFTIAPSLAPIALPAIPLGVPSDVLERRPDIASAERAMAAANA 308 RA EHA+A L G F++ P +P G+PS +LERRPDIA+AERAMAAANA Sbjct: 267 RATAEHALAILLGRTPAEFSLPPQPLTRLAVTVPPGLPSALLERRPDIAAAERAMAAANA 326 Query: 309 QIGVASAAFYPSFMLQPGYGVDSRNWGALFNAPSVLWSL----GVSASQSLFDAGRLRAG 364 ++GVA +AF+PS L G G +S G LFN S + L G + S LFD GR +AG Sbjct: 327 RVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAG 386 Query: 365 VDFSKAGYDATVATYRRTVLTAMQEVEDGITGIAALDRAYGQSQSAIASARRVLDIANSR 424 VD ++A Y+ VA+YR TVL A +EVED + + LD+ A+A++ R +++++ Sbjct: 387 VDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQ 446 Query: 425 YEGGVTPYLDVITAQQALLNSERQSAQLMGQRLLVSVFLIKALGGDW 471 Y G Y VI A + +L +R S L G+R +V LI+ALGG W Sbjct: 447 YREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 222 bits (566), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 159/479 (33%), Positives = 243/479 (50%), Gaps = 24/479 (5%) Query: 4 RSLLAASTVLTALMLAGCASGPDYVKPAVELPVAWTPEAPWRVMQPQDASPRGP------ 57 R L + ++ A +L GC+ P Y +P +AP P DA+ R Sbjct: 11 RLLHMCAALVMAGVLGGCSLAPTYERP----------QAPVESSYPADAAGRAELQAVNL 60 Query: 58 -WWERFHDARLNALQQQALAGNQTLAIASARLAQARAQLEVVGAAQSPQVGINTRAARAR 116 W + F D RL L AL N+ L A + +ARAQ ++ A P + ++ AAR R Sbjct: 61 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTR 120 Query: 117 ISANRPLTNYNSPNFSTVQNDFALGLNV-SYELDFFGRVRRSVEAASASAQQSAADLENT 175 +A++ LT T+ N + +GL V +YELDFFGRVR +AA AS + ++ Sbjct: 121 TAASQSLTGQ-----PTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSA 175 Query: 176 RLLLTAELASNYFNLRELDIELDVVQRAIALQRKALELATSRHDLGATSGLDVAQQQALL 235 ++ L A++A Y R +L + + ++ L+LA R D+GA++ LD Q L+ Sbjct: 176 QISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLV 235 Query: 236 DNTLTQVDILGRQRAQYEHAIATLTGTPAPGFTIAPSLAPIALPA-IPLGVPSDVLERRP 294 ++ L RQRAQ E+A+ L G A L+ + IP G+PSD+L RP Sbjct: 236 ESARVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRP 295 Query: 295 DIASAERAMAAANAQIGVASAAFYPSFMLQPGYGVDSRNWGALFNAPSVLWSLGVSASQS 354 DI SAE+++ AANA IG A AAF+P L +G S G LF + S WS G S S Sbjct: 296 DIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLP 355 Query: 355 LFDAGRLRAGVDFSKAGYDATVATYRRTVLTAMQEVEDGITGIAALDRAYGQSQSAIASA 414 +FD GR A +D ++ + V TY + + TA +EV D + +LD ++ +A+ Sbjct: 356 IFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQ 415 Query: 415 RRVLDIANSRYEGGVTPYLDVITAQQALLNSERQSAQLMGQRLLVSVFLIKALGGDWQE 473 + L + + R++ G+ LD + AQ+ L ++++ Q RL ++ L KALGG E Sbjct: 416 QERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNE 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.129 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,298,958 Number of extensions: 47576 Number of successful extensions: 285 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 226 Number of HSP's successfully gapped: 17 Length of query: 501 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 412 Effective length of database: 1,198,455 Effective search space: 493763460 Effective search space used: 493763460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)