BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4479.p (430 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 745 0.0 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 378 e-106 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 347 1e-96 gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 136 3e-33 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 128 1e-30 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 745 bits (1924), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/430 (87%), Positives = 403/430 (93%), Gaps = 2/430 (0%) Query: 1 MPHIASTPRHAGRRSSTAVRLALSLTMVAVLGACSRSGDQPKSAAQSEVGYVVVKAQRQA 60 M ASTPR GR SS RLALSL +VAVL ACSRSGDQPK AQ+EVGYVVVKAQRQA Sbjct: 1 MHPTASTPRRQGRTSSATARLALSLILVAVLAACSRSGDQPKPTAQAEVGYVVVKAQRQA 60 Query: 61 LTTELPGRTTAYLAADIRPQVGGIIQQRLFKEGSLVKAGQPLYQIDPASYQAAYDSAKAS 120 LTTELPGRTTAYLAADIRPQVGGIIQ+RLFKEGS VKAGQPLYQIDPASYQAAYDSA+AS Sbjct: 61 LTTELPGRTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARAS 120 Query: 121 LLKSEATLASSKLKAERYAELNKIDAVAKQDNDDTQSTMRQNQADVEVQKAAVQTAAINL 180 LLK+EATLASSKLKAERYAELNKIDAV+KQDNDD QST+RQNQADVEVQKAAVQTAAINL Sbjct: 121 LLKAEATLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINL 180 Query: 181 QYTRIVSPISGRIEQSTVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA 240 QYT+I+SPISGRIE+S VTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA Sbjct: 181 QYTKILSPISGRIEKSAVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA 240 Query: 241 SGQIRKSGANAAQVKILLEDGSTYDHPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPG 300 SGQIRKSG NAAQVKI+LEDGSTY+ PGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPG Sbjct: 241 SGQIRKSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPG 300 Query: 301 AYVRAVLEEGVDEHALLVPQKAISRDQTGAATALVVGTDGKVQQRVVTVARAVGNNWWIS 360 AYVRAVLEEGVD+HALL PQKA+SRDQTGAATALV+G DGKVQQR+V+VARAVGNNWW+S Sbjct: 301 AYVRAVLEEGVDDHALLAPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVS 360 Query: 361 KGLQEGDKLIVDGLQKVRVGDSPRAIDVTDSLQKDLAREAAAAAADPSLGGDAGKAAAAG 420 +GL+EGDKLIVDGLQKVR GD+PRAID+TDSLQKDLAREAAAAAADPSLGGDAGK +A Sbjct: 361 RGLKEGDKLIVDGLQKVRTGDAPRAIDITDSLQKDLAREAAAAAADPSLGGDAGKDSAPA 420 Query: 421 TAATSGAQSK 430 +A + AQSK Sbjct: 421 SA--TAAQSK 428 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 378 bits (971), Expect = e-106, Method: Compositional matrix adjust. Identities = 198/358 (55%), Positives = 261/358 (72%), Gaps = 2/358 (0%) Query: 24 SLTMVAVLGACSRSGDQPKSAAQSEV-GYVVVKAQRQALTTELPGRTTAYLAADIRPQVG 82 +L +++VL AC + AA + V G V V AQ L TELPGRT+ Y AD+RPQV Sbjct: 15 ALAILSVLSACGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGRTSPYQIADVRPQVN 74 Query: 83 GIIQQRLFKEGSLVKAGQPLYQIDPASYQAAYDSAKASLLKSEATLASSKLKAERYAELN 142 GII+ R F+EGS VKAGQ LYQI+PASYQAAYDS+ A+L K+EA++ +++LKA RY EL Sbjct: 75 GIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELV 134 Query: 143 KIDAVAKQDNDDTQSTMRQNQADVEVQKAAVQTAAINLQYTRIVSPISGRIEQSTVTPGA 202 I AV++QD DD +++ + +ADV +A VQT+ INL Y R+ +PISGRI +S+VTPGA Sbjct: 135 AIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGA 194 Query: 203 LVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGANAAQVKILLEDGS 262 LVTA+QTTALTT+QQ+DPIYVDVTQS+ L L+ LA+GQ+ KSG N A+V+++LE+G Sbjct: 195 LVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG-NGAKVRLVLENGR 253 Query: 263 TYDHPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDEHALLVPQKA 322 TY G L+F+ ++V+ TG ITLRA+ PNP LLPG YVRAVL+EGV + LLVPQ+A Sbjct: 254 TYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQA 313 Query: 323 ISRDQTGAATALVVGTDGKVQQRVVTVARAVGNNWWISKGLQEGDKLIVDGLQKVRVG 380 +SRD G A VV G V+QR + RA+G+ W +S GL+EGD+L+VDG Q+ G Sbjct: 314 VSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAG 371 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 347 bits (890), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 183/371 (49%), Positives = 253/371 (68%), Gaps = 12/371 (3%) Query: 16 STAVRLALSLTMVAVLGACSRSGDQPKSAAQ------SEVGYVVVKAQRQALTTELPGRT 69 S RL +++ + A L AC + K AAQ SEV V + QR A+T ELPGR Sbjct: 5 SRFTRLTMTVAIAAALVACGQ-----KPAAQGGPMPPSEVSVVTIAPQRVAITNELPGRV 59 Query: 70 TAYLAADIRPQVGGIIQQRLFKEGSLVKAGQPLYQIDPASYQAAYDSAKASLLKSEATLA 129 A+ A +R +V GI+Q+R+FKEGS VK GQ L++IDPA Y+A+ +SA+A+L +++A L Sbjct: 60 EAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLT 119 Query: 130 SSKLKAERYAELNKIDAVAKQDNDDTQSTMRQNQADVEVQKAAVQTAAINLQYTRIVSPI 189 + L A+RY L +++AV+KQ+ D+ + +Q +ADV KAAVQTA++NL Y + SPI Sbjct: 120 QTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPI 179 Query: 190 SGRIEQSTVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGA 249 SGRI ++ VT GALV T L VQQ+DP+YV+VTQS+ DLL+L++ +A+GQ++ G Sbjct: 180 SGRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGD 239 Query: 250 NAAQVKILLEDGSTYDHPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEE 309 N A V + LE+G Y PGKL F+ +SVD TG ++LRA PNP +LLPG YVRA +E+ Sbjct: 240 NKASVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQ 299 Query: 310 GVDEHALLVPQKAISRDQTGAATALVVGTDGKVQQRVVTVARAVGNNWWISKGLQEGDKL 369 VDE ALLVPQ+A+ RD G +T +VV + KV+ R V A GNNW + GL+EGD++ Sbjct: 300 AVDEQALLVPQQAVVRD-AGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRV 358 Query: 370 IVDGLQKVRVG 380 IV+GLQKVR G Sbjct: 359 IVEGLQKVRPG 369 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 136 bits (342), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 107/318 (33%), Positives = 163/318 (51%), Gaps = 10/318 (3%) Query: 67 GRTTAYLAADIRPQVGGIIQQRLFKEGSLVKAGQPLYQIDPASYQAAYDSAKASLLKSEA 126 GR A ++RP VGG + FK+G+LVK G L+ IDP Y+A A+A L +EA Sbjct: 69 GRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEA 128 Query: 127 TLASSKLKAERYAELNKIDAVAKQDNDDTQSTMRQNQADVEVQKAAVQTAAINLQYTRIV 186 + R L +A+AK+D ++ Q+ R+ A+V +AA++ A +NL+YTRI Sbjct: 129 RAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTRIT 188 Query: 187 SPISGRIEQSTVTPGALVTASQTTA-LTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIR 245 +P+SGRI ++ VT G +V A LTT+ + +Y L+ R Sbjct: 189 APVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKY-----VNPAR 243 Query: 246 KSGANA-AQVKILLEDGSTYDHPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVR 304 +GA ++ + EDG Y G++ +DT +G I +RA+ N + L+PG Y R Sbjct: 244 AAGATVPVELGLANEDG--YSRKGRVGSVDNRLDTASGTIRVRAIFDNANSQLVPGLYAR 301 Query: 305 AVLEEGVDEHALLVPQKAISRDQTGAATALVVGTDGKVQQRVVTVARAVGNNWWISKGLQ 364 L G A+L+ +KAI DQ +V D R V V I KGLQ Sbjct: 302 VRLGGGEPHQAVLIDEKAIGTDQD-KRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQ 360 Query: 365 EGDKLIVDGLQKVRVGDS 382 G++++V+GLQ++R GD+ Sbjct: 361 PGERIVVNGLQRIRPGDA 378 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 98/316 (31%), Positives = 155/316 (49%), Gaps = 12/316 (3%) Query: 67 GRTTAYLAADIRPQVGGIIQQRLFKEGSLVKAGQPLYQIDPASYQAAYDSAKASLLKSEA 126 G T A +R +V G + + +KEG +VKAG L +IDP S QAA A+ L + A Sbjct: 52 GAVTPTATATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRA 111 Query: 127 TLASSKLKAERYAELNKIDAVAKQDNDDTQSTMRQNQADVEVQKAAVQTAAINLQYTRIV 186 LAS++L +RY L D++A Q D + ++Q + V+ + V +A + L +TR+ Sbjct: 112 LLASARLDLKRYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVT 171 Query: 187 SPISGRIEQSTVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRK 246 +PISGR+ G VT S T L + Q+ PI + ++ ++ ++L SG+ Sbjct: 172 APISGRLGLRQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLP 231 Query: 247 SGANAAQVKILLEDGS--TYDHPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVR 304 + A + K L DG T D+ VD TG + L+A PN D L P +V Sbjct: 232 AQAWDREQKNKLADGVLLTIDN---------VVDATTGTVKLKAQFPNTDYALFPSQFVN 282 Query: 305 AVLEEGVDEHALLVPQKAISRDQTGAATALVVGTDGKVQQRVVTVARAVGNNWWISKGLQ 364 L+ ++ A ++P AI R G VV D V R V + I+ G+ Sbjct: 283 IRLQLNTEQGATVIPTAAIQRGSKGLFV-YVVKDDSSVTVRPVKTGPVQDDLTVITDGVS 341 Query: 365 EGDKLIVDGLQKVRVG 380 G+ +++DG+ ++R G Sbjct: 342 AGETVVIDGIDRLREG 357 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.341 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,141,090 Number of extensions: 45380 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 12 Length of query: 430 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 342 Effective length of database: 1,203,190 Effective search space: 411490980 Effective search space used: 411490980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)