BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4477.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 841 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 517 e-148 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 415 e-117 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 336 3e-93 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 199 4e-52 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 841 bits (2173), Expect = 0.0, Method: Compositional matrix adjust. Identities = 430/493 (87%), Positives = 459/493 (93%), Gaps = 1/493 (0%) Query: 1 MNRPVQQKPTLWPRTAMILALTAALAGCANLEPDYARPVAPVPASWTQQIDGKTVVSATG 60 M +PVQ+K TLWPRTAM+LALTAALAGCANL PDY +P APVPASWT Q + V + G Sbjct: 1 MMQPVQRKSTLWPRTAMLLALTAALAGCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAG 60 Query: 61 KV-GQSDADQLGWRQFFLDPRLQQVIAAALENNRDLRVAVLNIEKARAQYRITDADRYPS 119 G+ DAD LGWR+FFLDPRLQQVIAAAL+NNRDLRVA LNIEKARAQYRITDA+RYPS Sbjct: 61 PAAGEIDADHLGWREFFLDPRLQQVIAAALDNNRDLRVAALNIEKARAQYRITDANRYPS 120 Query: 120 ISATGSSSASRTPAELSSTGLATVSHQQSLTVGMSAYELDFFGRLKNLSDAALETYQYQV 179 ISATG+SSASRTPAELS+TG A VSHQQS+TVG+SAYELDFFGRLKNLSDAALE+YQYQV Sbjct: 121 ISATGTSSASRTPAELSTTGSAAVSHQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQV 180 Query: 180 ETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYTSQSRTYELTQRSHDIGTVSGVDLA 239 ETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTY SQSRT+ L+QRSHDIGTVSGVDLA Sbjct: 181 ETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLA 240 Query: 240 SLEATVEAARADVASYTSQVAQDINALRLLVGADVDAALLPTGLPDTASVLPATPAGLPS 299 S EATVE+ARADVA Y SQV QD+NALRLLVGAD+DAALLP GLPDTASVLPATPAGLPS Sbjct: 241 SQEATVESARADVARYASQVEQDLNALRLLVGADIDAALLPAGLPDTASVLPATPAGLPS 300 Query: 300 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSITLTADGGTASRTLGGLFKPGSGSWSF 359 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSI+LTA+GGTASRTLGGLFK GSGSW+F Sbjct: 301 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTF 360 Query: 360 APSINLPIFNSGSLRASLDSAKITRDIDVANYEKAIQTAFSEVADALAVRATLDQRLDAQ 419 +PSINLPIFN+GSLRASLDSAKITRDIDVANYEKAIQTAF EVADALAVRATLDQRLDAQ Sbjct: 361 SPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQ 420 Query: 420 GKATAASEKAFRLSEARYKNGIDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL 479 GKATAASEKAFRLS+ARY+NG+DSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL Sbjct: 421 GKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL 480 Query: 480 GGGWNEQTVQKAP 492 GGGW+EQT P Sbjct: 481 GGGWSEQTQPMQP 493 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 517 bits (1332), Expect = e-148, Method: Compositional matrix adjust. Identities = 282/488 (57%), Positives = 346/488 (70%), Gaps = 12/488 (2%) Query: 8 KPTLWPRT----AMILALTAALAGCANLEPDYARPVAPVPASWTQQIDGKTVVSATGKVG 63 KP+L R+ M+ + A L G L PDY RP APV A + D K A+ Sbjct: 2 KPSLSTRSPVLPMMLPVMLAMLLGGCTLMPDYHRPDAPVAAHFAG-ADSKNAAGASSAPV 60 Query: 64 QSDADQLGWRQFFLDPRLQQVIAAALENNRDLRVAVLNIEKARAQYRITDADRYPSISAT 123 +D +GWR F DP LQQVIA ALENNRDLRVAVLNIEKARAQY + A +PSI A Sbjct: 61 VAD---IGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAA 117 Query: 124 GSSSASRTPAELSSTGLATVSHQQSLTVGMSAYELDFFGRLKNLSDAALETYQYQVETRR 183 S +ASRTP +LSSTG A S + T+G SAYELD FGR+++L + AL+++ E RR Sbjct: 118 TSETASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARR 177 Query: 184 STHISLVAEVATAWLTLASDRDLLKLAQDTYTSQSRTYELTQRSHDIGTVSGVDLASLEA 243 ST ISL+AEVATAWLTLASD+D L+LAQDT SQS +Y LTQRS ++G+ S + L + Sbjct: 178 STQISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQT 237 Query: 244 TVEAARADVASYTSQVAQDINALRLLVGADVDAALLPTGLPDT----ASVLPATPAGLPS 299 +VE+AR DV SYT+QV QD NAL LLVG D+ ALLP GLPDT AS L P GLPS Sbjct: 238 SVESARVDVESYTAQVDQDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPS 297 Query: 300 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSITLTADGGTASRTLGGLFKPGSGSWSF 359 +L RR D++ AE L++ANA+IGAARAAF+PSITLT + GTAS L GLFK GSGSWSF Sbjct: 298 DLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSF 357 Query: 360 APSINLPIFNSGSLRASLDSAKITRDIDVANYEKAIQTAFSEVADALAVRATLDQRLDAQ 419 P I+LPIF+ G+ +A+LD A ++RDI VA YEKAIQTAF EV+DALA R TL ++L AQ Sbjct: 358 VPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQ 417 Query: 420 GKATAASEKAFRLSEARYKNGIDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL 479 AS++AFRLS+ARY +G+DSYLT L +QRTLYSAQ+ LI +L+ +N +T YK L Sbjct: 418 EALVQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTL 477 Query: 480 GGGWNEQT 487 GGGW E+T Sbjct: 478 GGGWVERT 485 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 415 bits (1066), Expect = e-117, Method: Compositional matrix adjust. Identities = 234/483 (48%), Positives = 309/483 (63%), Gaps = 17/483 (3%) Query: 12 WPRTAMILALTAAL------AGCANLEPDYARPVAPVPASWTQQIDGKTVVSATGKVGQS 65 PR+ +L + AAL GC+ L P Y RP APV +S+ G+ + A Sbjct: 6 MPRSPRLLHMCAALVMAGVLGGCS-LAPTYERPQAPVESSYPADAAGRAELQAVN----- 59 Query: 66 DADQLGWRQFFLDPRLQQVIAAALENNRDLRVAVLNIEKARAQYRITDADRYPSISATGS 125 LGWRQFF D RLQQ+IAAALENNRDLR AVLNIE+ARAQY+I AD P+++ +G+ Sbjct: 60 ----LGWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGN 115 Query: 126 SSASRTPAELSSTGLATVSHQQSLTVGMSAYELDFFGRLKNLSDAALETYQYQVETRRST 185 ++ +RT A S TG T+S+ + + + AYELDFFGR++NL DAAL +Y E ++S Sbjct: 116 AARTRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSA 175 Query: 186 HISLVAEVATAWLTLASDRDLLKLAQDTYTSQSRTYELTQRSHDIGTVSGVDLASLEATV 245 ISLVA+VA A+L S + LKLA +T S+ +L Q+ D+G + +D + V Sbjct: 176 QISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLV 235 Query: 246 EAARADVASYTSQVAQDINALRLLVGADVDAALLPTGLPDTASVLPATPAGLPSQVLLRR 305 E+AR Q AQ NAL LLVG + L P L ++ PAGLPS +L R Sbjct: 236 ESARVAKTQLERQRAQAENALTLLVGKKI-GELPPAQLLSDEKIVTDIPAGLPSDLLTNR 294 Query: 306 PDVLAAEHTLKSANADIGAARAAFFPSITLTADGGTASRTLGGLFKPGSGSWSFAPSINL 365 PD+ +AE +L +ANA+IGAARAAFFP I+LT + G+AS TLGGLF+ GSG+WSF PS++L Sbjct: 295 PDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSL 354 Query: 366 PIFNSGSLRASLDSAKITRDIDVANYEKAIQTAFSEVADALAVRATLDQRLDAQGKATAA 425 PIF+ G A+LD A++ ++ V YEKAIQTAF EVADAL R +LD+++ AQ AA Sbjct: 355 PIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAA 414 Query: 426 SEKAFRLSEARYKNGIDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWNE 485 ++ +L++ R+KNGI S L L AQR L+SAQQ LI R +N I LYK LGGG NE Sbjct: 415 QQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNE 474 Query: 486 QTV 488 TV Sbjct: 475 NTV 477 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 336 bits (861), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 205/457 (44%), Positives = 278/457 (60%), Gaps = 10/457 (2%) Query: 28 CANLEPDYARPVAPVPASWTQQIDGKTVVSATGKVGQSDADQLGWRQFFLDPRLQQVIAA 87 C +L P Y RP APV A + DG +A ++ + L WR +F DP LQ +I Sbjct: 25 CQSLAPHYQRPAAPVAAVYP---DGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQ 81 Query: 88 ALENNRDLRVAVLNIEKARAQYRITDADRYPSISATGSSSASRTPAELSSTGLATVSHQQ 147 ALEN+RDLR A L +++A+A Y I AD +P+I A SRTPA+L+ T V Sbjct: 82 ALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAY 141 Query: 148 SLTVGMSAYELDFFGRLKNLSDAALETYQYQVETRRSTHISLVAEVATAWLTLASDRDLL 207 +G+S++E+D +GRL++L DAALE+ + TR + +SL+A+VA AWL LA + L Sbjct: 142 QAGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERL 201 Query: 208 KLAQDTYTSQSRTYELTQRSHDIGTVSGVDLASLEATVEAARADVASYTSQVAQDINALR 267 ++A T S+ ++ + R ++G S ++L +E + A+A A Q AQ +NAL Sbjct: 202 EIANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALT 261 Query: 268 LLVGADVDAALLPTGLPDT---ASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGA 324 LLVG+ V +LP G A P P GLP+ +L RRPDV AAEH L +ANA+IGA Sbjct: 262 LLVGSPV---VLPVGQARAGLDAVFAPLQP-GLPALLLTRRPDVAAAEHRLMAANANIGA 317 Query: 325 ARAAFFPSITLTADGGTASRTLGGLFKPGSGSWSFAPSINLPIFNSGSLRASLDSAKITR 384 ARAAFFPSI+LT + GTAS L GLF+ GS +W+F+PSI LPIF +G LR +L +++ R Sbjct: 318 ARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRR 377 Query: 385 DIDVANYEKAIQTAFSEVADALAVRATLDQRLDAQGKATAASEKAFRLSEARYKNGIDSY 444 D+ VA YEK +QTAF EVADALA R L Q+L+ A + RLS RY NG + Sbjct: 378 DLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPF 437 Query: 445 LTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGG 481 L L AQR L SAQQ L+ +R S+ + LY LGG Sbjct: 438 LDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 199 bits (506), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 155/468 (33%), Positives = 235/468 (50%), Gaps = 20/468 (4%) Query: 22 TAALAGCANLEPDYARPVAPVPASWTQQID-----GKTVVSATGKVGQSDADQLGWRQFF 76 T AL CA L AP+P S +D +T+ A +GW+ +F Sbjct: 14 TLALCACAPL-------TAPLPPS---ALDLPTHYPRTLAEDAPANAAPTASAIGWQDYF 63 Query: 77 LDPRLQQVIAAALENNRDLRVAVLNIEKARAQYRITDADRYPSISATGSSSASRTPAELS 136 LQ +IA AL +N DLR L +++ +AQ+RI + P+++ + SR P E Sbjct: 64 PQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPP 123 Query: 137 STGLATVSHQQSLTVGMSAYELDFFGRLKNLSDAALETYQYQVETRRSTHISLVAEVATA 196 +G A + + + +G S++ELDF+GR+ +L++AAL+ + ++ ISL+ +VA A Sbjct: 124 ESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAA 183 Query: 197 WLTLASDRDLLKLAQDTYTSQSRTYELTQRSHDIGTVSGVDLASLEATVEAARADVASYT 256 W +L + L L + + + + L Q HD G LA + + RA+ A Sbjct: 184 WTSLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQ 243 Query: 257 SQVAQDINALRLLVG-ADVDAALLPTGLPDTASVLP-ATPAGLPSQVLLRRPDVLAAEHT 314 A + L +L G + AAL P T +LP A GLPS +L RPD++AAEH Sbjct: 244 QMRASQRSTLEMLAGLTQLPAALEPA---PTTILLPFALAPGLPSTLLAARPDIIAAEHR 300 Query: 315 LKSANADIGAARAAFFPSITLTADGGTASRTLGGLFKPGSGSWSFAPSINLPIFNSGSLR 374 L+++ A + AARAAFFP I+LTA GG AS L LFK S +W+F P I+LP+ + G + Sbjct: 301 LQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQ 360 Query: 375 ASLDSAKITRDIDVANYEKAIQTAFSEVADALAVRATLDQRLDAQGKATAASEKAFRLSE 434 + D A + + + +YE+ +Q AF EVA L L ++L + + + Sbjct: 361 SRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMH 420 Query: 435 ARYKNGIDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGG 482 R + G S L L ++R AQQ L N+I LY LGGG Sbjct: 421 MRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGG 468 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,341,895 Number of extensions: 50840 Number of successful extensions: 299 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 256 Number of HSP's successfully gapped: 17 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)