BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4474.p (418 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 803 0.0 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 454 e-129 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 161 8e-41 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 128 9e-31 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 93 4e-20 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 803 bits (2073), Expect = 0.0, Method: Compositional matrix adjust. Identities = 403/418 (96%), Positives = 413/418 (98%) Query: 1 MKYVRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMNTQQYSYIVVAWQICYAV 60 MKYVRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDM+TQQYSYIVVAWQICYAV Sbjct: 1 MKYVRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAV 60 Query: 61 MQPVAGYVLDAVGTKIGFGIFALAWSLVCAAAAMATGWQSLAFFRALLGMTEAAGIPGGV 120 MQPVAGY+LDAVGTKIGFGIFALAWSLVCAAAA ATGWQSLAFFRALLG+TEAAGIPGGV Sbjct: 61 MQPVAGYILDAVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGV 120 Query: 121 KASTEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFL 180 KASTEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVG LGV+WF L Sbjct: 121 KASTEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVL 180 Query: 181 WMLFYKSPRDQKLLSPEERAYILDGQEKAPEKVQRESWTKIVRSQNFWSIAIPRFLSEPA 240 WMLFYKSPRDQKLLSPEERAYIL+GQEK+PEKVQRESWTKIVRS+NFWSIAIPRFLSEPA Sbjct: 181 WMLFYKSPRDQKLLSPEERAYILEGQEKSPEKVQRESWTKIVRSRNFWSIAIPRFLSEPA 240 Query: 241 WQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSR 300 WQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSR Sbjct: 241 WQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSR 300 Query: 301 KLVMLVGSLAMIGPACVGFVDSPYVAIGLLSIGGFAHQTLSGALYSITSDVFTRNQVATA 360 KLVMLVGSL+MIGPACVGFVDSPYVAI LLSIGGFAHQTLSGALYSITSDVFT+NQVATA Sbjct: 301 KLVMLVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATA 360 Query: 361 TGLTGMSGYLGATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPEVLA 418 TGLTGMSGYLGATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPE +A Sbjct: 361 TGLTGMSGYLGATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPEAIA 418 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 454 bits (1169), Expect = e-129, Method: Compositional matrix adjust. Identities = 217/412 (52%), Positives = 294/412 (71%), Gaps = 1/412 (0%) Query: 1 MKYVRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMNTQQYSYIVVAWQICYAV 60 MK ++G+RW+++ LV L I NYLARN+L V APT+ EL M+TQQYSY+V A+Q+ Y + Sbjct: 1 MKTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI 60 Query: 61 MQPVAGYVLDAVGTKIGFGIFALAWSLVCAAAAMATGWQSLAFFRALLGMTEAAGIPGGV 120 MQPV G+++D +G +IGF +F + WS+ A ATGW SLA FRAL+G+TEAA IP G+ Sbjct: 61 MQPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM 120 Query: 121 KASTEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFL 180 KA EWFP KE+SVA+GWFN G+S+GA+ APPLV++ + GW+ +FVV G+LG +W L Sbjct: 121 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAAL 180 Query: 181 WMLFYKSPRDQKLLSPEERAYILDGQEKAPEKVQRESWTK-IVRSQNFWSIAIPRFLSEP 239 W FY+SPRD LS ER I+ GQ + V+ + K +VRS+ FW+IA+ RF +EP Sbjct: 181 WFAFYRSPRDHAALSDSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEP 240 Query: 240 AWQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTS 299 AWQTF+ WIPLY ERH+++K IAMFAWLPFLAAD+G + GGYLSP ++ L S Sbjct: 241 AWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWS 300 Query: 300 RKLVMLVGSLAMIGPACVGFVDSPYVAIGLLSIGGFAHQTLSGALYSITSDVFTRNQVAT 359 R +++G++ MIGPACVG V SPYVAI L +GGFAHQ +SG + ++++DVF +V T Sbjct: 301 RVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGT 360 Query: 360 ATGLTGMSGYLGATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIAR 411 A+G GMS ++G F+LL G L +GYGPLF +L AFDL+ ++ + R Sbjct: 361 ASGFAGMSAWIGGLGFSLLVGALADTVGYGPLFGMLGAFDLIGAVLLIILIR 412 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 161 bits (408), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 111/380 (29%), Positives = 183/380 (48%), Gaps = 10/380 (2%) Query: 12 VTLVTLALIVNYLARNTLSVAAPTMMKELDMNTQQYSYIVVAWQICYAVMQPVAGYVLDA 71 ++ + LA IVNYL R+TLS+A ++ +EL+++ Q ++ A+ + YA Q G +LD Sbjct: 19 LSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIGAMLDK 78 Query: 72 VGTKIGFGIFALAWSLVCAAAAMATGWQSLAFFRALLGMTEAAGIPGGVKASTEWFPAKE 131 G ++ G WS+ A Q R LG+ EA P G K +EWF +E Sbjct: 79 FGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVVSEWFAMRE 138 Query: 132 RSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFLWMLFYKSPRDQ 191 R G F S+IG APP++ +L GW+M FV++GVLG+ W LFY++ R++ Sbjct: 139 RGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRN-RNE 197 Query: 192 KLLSPEERAYILDGQEKAPEKVQRESWTK---IVRSQNFWSIAIPRFLSEPAWQTFNAWI 248 L+P E Y+ D + P + SW++ + + W + + W+ Sbjct: 198 VQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWL 257 Query: 249 PLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRK---LVML 305 P Y+ ERH++I +P++ +G + GY++ K + SRK L Sbjct: 258 PAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSRKWPICAGL 316 Query: 306 VGSLAMIGPACVGFVDSPYVAIGLLSIGGFAHQTLSGALYSITSDVFTRNQVATATGLTG 365 +G+ A PA + S +AI +S+ SG +++ S R VA+ + Sbjct: 317 IGAGAFTIPAA--YTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLVASLGSMQN 374 Query: 366 MSGYLGATLFTLLFGILVTQ 385 GYLG +L ++ G++V Q Sbjct: 375 FGGYLGGSLAPVITGVVVDQ 394 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 128 bits (321), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 104/401 (25%), Positives = 182/401 (45%), Gaps = 27/401 (6%) Query: 7 IRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMNTQQYSYIVVAWQICYAVMQPVAG 66 +R+ ++ L+ + VNY R TLS+A M EL ++ YI A+ Y + Q G Sbjct: 11 VRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGG 70 Query: 67 YVLDAVGTKIGFGIFALAWSL----------VCAAAAMATGWQSLAFFRALLGMTEAAGI 116 ++LD G+K + WS+ + AAA+A SL R ++G+ EA Sbjct: 71 WLLDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVA----SLFLLRFMVGLAEAPSF 126 Query: 117 PGGVKASTEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVL 176 P + WFP KER A FN A+ PL+ W + W F V+GV+G+ Sbjct: 127 PANGRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIA 186 Query: 177 WFFLWMLFYKSPRDQKLLSPEERAYILDG---------QEKAPEKVQRESWTKIVRSQNF 227 +W SP+D ++ E A+I G + AP R +++ ++ Sbjct: 187 LALIWGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRML 246 Query: 228 WSIAIPRFLSEPAWQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPL 287 + I ++ F W P+Y+ ER M+I + A +P + IG V+GG +S Sbjct: 247 LGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDR 306 Query: 288 FHKHLKVSLFTSRKLVMLVGSLAMIGPACVGFVDSPYVAIGLLSIGGFAHQTLSGAL-YS 346 K +S+ +RK+ ++ G L + +V++ ++ +G++++ F GAL ++ Sbjct: 307 LIKR-GLSVTWARKIPIVGGMLLSMTMILCNYVEAQWMVVGIMALAFFGKGV--GALGWA 363 Query: 347 ITSDVFTRNQVATATGLTGMSGYLGATLFTLLFGILVTQIG 387 + +D + V + GL M G + ++ G +V + G Sbjct: 364 VVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETG 404 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 93.2 bits (230), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 84/333 (25%), Positives = 143/333 (42%), Gaps = 31/333 (9%) Query: 8 RWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMNTQQYSYIVVAWQICYAVMQPVAGY 67 RW + L+ + V R L VA P + KE M+ + + + Y++ Q +G Sbjct: 16 RWVVGALIFIVYTVAAADRANLGVALPFLRKEFVMSNTEAGALASIFLFAYSLAQLPSGL 75 Query: 68 VLDAVGTKIGFGIFALAWSLVCAAAAMATGWQSLAFFRALLGMTEAAGIPGGVKAS-TEW 126 G F + LA S+ A+ +L FR LG+ E +P G+ ++ W Sbjct: 76 AYTRFGIPRVFSLAMLATSVFTGLIGTASSLLALKIFRFGLGLAEGP-LPIGITSTINNW 134 Query: 127 FPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFLWMLFYK 186 FPA+E+ G F + G + PP+ + + GW+ F V GV+ +W++ K Sbjct: 135 FPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIFYFFAVPGVILSVVWLMVVK 194 Query: 187 S-PRDQKLLSPEERAYILD------GQEKAP---------EKVQRESWTKIVRSQ----- 225 + P + +S EE +I D GQ+ P +++ R S + + Sbjct: 195 NRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLDRIIRRSKVATIETTKGVFC 254 Query: 226 --NFWSIAIPRFLSEPAWQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGY 283 N A+ F AWIP Y+ + + +I + M A P++ A IG +LGG Sbjct: 255 SWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANMGMVAAAPWVGAVIGNLLGGA 314 Query: 284 LSPL-FHKHLKVSL-----FTSRKLVMLVGSLA 310 ++ K K ++ FT+ +V+L+ S A Sbjct: 315 MTDRWLEKRRKPAMMMSAFFTTIMMVILINSPA 347 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,186,340 Number of extensions: 44253 Number of successful extensions: 267 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 21 Length of query: 418 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 330 Effective length of database: 1,203,190 Effective search space: 397052700 Effective search space used: 397052700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)