BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4469.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313879|ref|YP_003777971.1| major facilitator superfamily p... 756 0.0 gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 426 e-120 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 91 2e-19 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 89 5e-19 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 86 5e-18 >gi|300313879|ref|YP_003777971.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 413 Score = 756 bits (1951), Expect = 0.0, Method: Compositional matrix adjust. Identities = 395/413 (95%), Positives = 406/413 (98%) Query: 1 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGMISGATLV 60 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAG+ISGATLV Sbjct: 1 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLV 60 Query: 61 ASAIGGWFAGALSDRYGRVKTLQITIIWFSLFTFLSAFAQSYPQLLILKALQGFGFGGEW 120 ASAIGGWFAGALSDRYGRVKTLQITIIWFS FTFLSAFAQS+PQLL+LKALQGFGFGGEW Sbjct: 61 ASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEW 120 Query: 121 AAGAVLMAETIRAEHRGKAMGSVQSAWAVGWGGAVLLFSVLFTMLPQEMAWRVMFVIGLL 180 AAGAVLMAETIRAEHRGKAMG+VQSAWAVGWGGAVLLFSVLF++LPQEMAWRVMFVIGLL Sbjct: 121 AAGAVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLL 180 Query: 181 PALLVVYVRRSVPETEAFLAGQKAAAGKPATSTLLGIFRPDVLRLTIIGSLLGVGAHGGY 240 PALLVVYVRRSV ET+AFLAGQKAAA KPA STLLGIFRPDVLRLTIIGSLLGVGAHGGY Sbjct: 181 PALLVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGY 240 Query: 241 YALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIVSAQLLDRIGRRRTVAFFALCC 300 YALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCI S+QLLDRIGRRRTVAFFAL C Sbjct: 241 YALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALAC 300 Query: 301 VATVLCYIFLPLSDTQMLVCGFPLGFFAAGIPASLGALFNELYPSGIRGTGVGFCYNFGR 360 VATVLCYIFLPLSDTQMLVCGFPLGFFAAGIPAS+GALFNELYPSGIRGTGVGFCYNFGR Sbjct: 301 VATVLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGR 360 Query: 361 VASAGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVIAVLMLPETRGRKMETGA 413 VASAGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVV+AVLMLPET+GRKMET A Sbjct: 361 VASAGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGRKMETSA 413 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 426 bits (1094), Expect = e-120, Method: Compositional matrix adjust. Identities = 213/407 (52%), Positives = 282/407 (69%) Query: 4 WYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGMISGATLVASA 63 W ++ T ER+T A F+G+A+D D +++ IP L+AA+S+SKA+AG I+ L+ SA Sbjct: 2 WLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSA 61 Query: 64 IGGWFAGALSDRYGRVKTLQITIIWFSLFTFLSAFAQSYPQLLILKALQGFGFGGEWAAG 123 IGGW AG L+DRYGRV+ LQ T++WF+ FTFLS F S+ QL +A+QGFG GGEWA G Sbjct: 62 IGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAVG 121 Query: 124 AVLMAETIRAEHRGKAMGSVQSAWAVGWGGAVLLFSVLFTMLPQEMAWRVMFVIGLLPAL 183 +VL+AETI A HRGKA G VQS+WAVGW G+ L F ++ +LP E AW+V+F IG+LPAL Sbjct: 122 SVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPAL 181 Query: 184 LVVYVRRSVPETEAFLAGQKAAAGKPATSTLLGIFRPDVLRLTIIGSLLGVGAHGGYYAL 243 L +Y+RR++ E +LA Q L IF D+LR T++ SLL G G YY++ Sbjct: 182 LTLYIRRAISEPAVYLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYSV 241 Query: 244 MTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIVSAQLLDRIGRRRTVAFFALCCVAT 303 TWLPT+LKTE+HLSVL T YL V+I +CG + SA L D IGRRR FA + Sbjct: 242 TTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIVL 301 Query: 304 VLCYIFLPLSDTQMLVCGFPLGFFAAGIPASLGALFNELYPSGIRGTGVGFCYNFGRVAS 363 VL Y + ++D ML GF LGFF +GI + +GA +EL+PS +RG+G GFCYNFGR Sbjct: 302 VLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAVG 361 Query: 364 AGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVIAVLMLPETRGRKME 410 A P LVG++S+S+PLGQ IG AA Y LV++A L+LPETRG+++E Sbjct: 362 AICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELE 408 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 95/335 (28%), Positives = 149/335 (44%), Gaps = 33/335 (9%) Query: 56 GATLVASAIGGWFAGALSDRYGRVKTLQITIIWFSLFTFLSAFAQSYPQLLIL------- 108 G + +G GA SDR GR L ++I+ ++ T + AF SY + +L Sbjct: 67 GVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIAL 126 Query: 109 -KALQGFGFGGEWAAGAVLMAETIRAEHRGKAMGSVQSAWAVGWGGAVLLFSVLF----- 162 K +QGF GGE+ + + E A HR S+W V G LL + LF Sbjct: 127 GKMIQGFSAGGEFGSSTAFLVE--HAPHRRGFF----SSWQVASQGISLLLAALFGAVLN 180 Query: 163 -TMLPQEMA---WRVMFVIGLLPALLVVYVRRSVPETEAFLAGQKAAAGKPATSTLLGIF 218 + P+++A WRV F+ GLL A +Y+RR + E F K ++ K + L F Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF----KESSEK-TNAPLRDTF 235 Query: 219 RPDVLRLTIIGSLLGVGAHGGYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCI 278 +RL I + + Y +L ++PTY + L S+ ++V + + G Sbjct: 236 AHQKMRLLIGAGSVIMATVSVYLSL--YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSP 293 Query: 279 VSAQLLDRIGRRR-TVAFFALCCVATVLCYIFLPLSD--TQMLVCGFPLGFFAAGIPASL 335 + L D+IGR + AL V T ++FL S Q+L+ +G A++ Sbjct: 294 LVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAM 353 Query: 336 GALFNELYPSGIRGTGVGFCYNFGRVASAGFPVLV 370 AL +++P RGTG+ YN GF L+ Sbjct: 354 PALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLI 388 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 89.4 bits (220), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 111/422 (26%), Positives = 180/422 (42%), Gaps = 49/422 (11%) Query: 23 WALDALDVQMFSLAIPALIAAFSLSKADAGMISGATLVASAIGGWFAGALSDRYGRVKTL 82 W DA+ V + P + F L+ A V +G + G L+DR GR L Sbjct: 32 WLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFGRLADRIGRRPVL 91 Query: 83 QITIIWFSLFTFLSAFAQSYPQLLILKALQGFGFGGEWAAGAVLMAETIRAEHRGKAMGS 142 ++ + F SAFA + LL L+ L G G GG +MAE + + RG+ + Sbjct: 92 MGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVM 151 Query: 143 VQSAWAVGWGGAVLLFSVLFTMLPQEMAWRVMFVIGLLPALLVVYVRRSVPETEAFL--A 200 ++S WA+ G L L + AWRV+F + LPAL+ V +R VPE+ +L + Sbjct: 152 LESFWAI--GTICLAVLALVAVSWGNDAWRVIFFVTGLPALVGVVLRFYVPESPMYLNRS 209 Query: 201 GQKAAAGK--------PATSTLLGIFRPDVLRLTIIGSLLGVGAHG-------------- 238 G+ AA + +ST + +P+V+ + +L Sbjct: 210 GKSDAARQVLERVAKVNGSSTPIPPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISI 269 Query: 239 GYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIVSAQLLDRIGRRRT-VAFFA 297 YY + +LP L +E + +L + + G +SA ++R GR+ T V F Sbjct: 270 AYYGVFVYLPVKLGSE-GFGFMRGQIFLIFLALVQLPGYALSAYGVERWGRKPTLVGFLV 328 Query: 298 LCCVATVLCYIFLPLSDTQMLVCGFPLGFFAAGIP-ASLGALFNELYPSGIRGTGVGFCY 356 L V C ++ D ++V L F+ A+L A E+YP+ +R +G+G Sbjct: 329 LSAVG---CMLYSLGKDPMLVVGSTLLLSFSLLGTWAALYAFTPEVYPTDLRASGMG--- 382 Query: 357 NFGRVASAG--------FPVLVGHMSDSMPLGQAIGIDAAIAYSLVVIAVLMLPETRGRK 408 G VA G PV+ H + ++ + A + A+A V + E+R R Sbjct: 383 TAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAFLVAGAVAILCVDV------ESRNRA 436 Query: 409 ME 410 +E Sbjct: 437 LE 438 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 85.9 bits (211), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 77/304 (25%), Positives = 126/304 (41%), Gaps = 42/304 (13%) Query: 26 DALDVQMFSLAIPALIAAFSLSKADAGMISGATLVASAIGGWFAGALSDRYGRVKTLQIT 85 DA + +P L+ + LS A G++ + + IG F G +++RYGRV + +T Sbjct: 56 DAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVT 115 Query: 86 IIWFSLFTFLSAFAQSYPQLLILKALQGFGFGGEWAAGAVLMAETIRAEHRGKAMGSVQS 145 + S+ + + A S+ L + + LQG G GGE A + E +A RGK + Sbjct: 116 VGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYEL 175 Query: 146 AWAVGWGGAVLLFSVLFTMLPQEMAWRVMFVIGLLPALLVVYVRRSVPETEAFLAGQ--- 202 + +G +L + + L W MF++G +P +LV + +PE+ +L + Sbjct: 176 IFPLG----LLAAAQVGAFLVPRFGWEYMFLVGGIPGILVALLITRLPESPRWLIARGRL 231 Query: 203 -----------------------------------KAAAGKPATSTLLGIFRPDVLRLTI 227 A AGK + +F P TI Sbjct: 232 DDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWKELFSPVYRGRTI 291 Query: 228 IGSLLGVGAHGGYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIVSAQLLDRI 287 I LL ++ + WLP+ KT HL + + +V V C + A L+DR+ Sbjct: 292 IVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCALLIDRV 351 Query: 288 GRRR 291 GRRR Sbjct: 352 GRRR 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,287,286 Number of extensions: 56943 Number of successful extensions: 397 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 352 Number of HSP's successfully gapped: 31 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)