BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4463.p (176 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoa... 240 5e-65 gi|300310060|ref|YP_003774152.1| nicotinamidase-like protein [He... 64 9e-12 gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily prot... 58 3e-10 >gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 176 Score = 240 bits (613), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 117/176 (66%), Positives = 135/176 (76%) Query: 1 MTSALLIIDVQQALCSGADAVANAEQTIASINLLSQQARTAGVPVIFVQHADHGALTHDS 60 M +ALLIID+Q ALC G DAV N+ I +N L+ QAR AG PVIFVQH D AL S Sbjct: 1 MPTALLIIDIQHALCHGPDAVENSAAVIDRVNQLADQARAAGAPVIFVQHEDEDALQRGS 60 Query: 61 PGWQLAAGLHHQADDLYLGKRGSDAFHQTQLEALLQSRGVRQLVICGMQTEFCVESTVRR 120 GWQL GLH Q +DL+L KRGSDAFH T+L+A+L+ R + LVICGMQTE+CVESTVRR Sbjct: 61 EGWQLMPGLHQQPEDLHLPKRGSDAFHHTELQAMLKMRHISALVICGMQTEYCVESTVRR 120 Query: 121 ALALGFPVTLAAGAHTTAPNGVLSVADVIAHHQTTLANLGAYGVRTTITAASDIRF 176 ALALG+PV LAA AHTTAPNGVL+VA VIAHH TLA LG+YGVR +I +DIRF Sbjct: 121 ALALGYPVMLAADAHTTAPNGVLTVAQVIAHHHATLAGLGSYGVRASIRPVADIRF 176 >gi|300310060|ref|YP_003774152.1| nicotinamidase-like protein [Herbaspirillum seropedicae SmR1] Length = 202 Score = 63.5 bits (153), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 43/127 (33%), Positives = 62/127 (48%), Gaps = 3/127 (2%) Query: 3 SALLIIDVQQALCSGADAVANAEQTIASINLLSQQARTAGVPVI-FVQHADHGALTHDSP 61 +AL+IID Q +G +A +A + LL + AR A PVI V H G D Sbjct: 23 AALVIIDHQTEYTTGRAPLAGIGAAVAEVALLLEHARAAAAPVIHVVHHGKPGGAMFDPQ 82 Query: 62 GWQLAA--GLHHQADDLYLGKRGSDAFHQTQLEALLQSRGVRQLVICGMQTEFCVESTVR 119 G +A G+ Q + + K ++F T L+ L+ G + L+I G T CV +TVR Sbjct: 83 GPHVAIIDGVQPQPGEAVITKGLPNSFAGTTLDETLKKTGRKSLIIAGFATHMCVSATVR 142 Query: 120 RALALGF 126 AL G+ Sbjct: 143 SALDHGY 149 >gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 58.2 bits (139), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 26/71 (36%), Positives = 41/71 (57%) Query: 56 LTHDSPGWQLAAGLHHQADDLYLGKRGSDAFHQTQLEALLQSRGVRQLVICGMQTEFCVE 115 L PGW++ L A ++ + K G +F T LE +L +RG+R +V+ G+ T+ CV Sbjct: 115 LVRGEPGWEIIPELAPIAGEIIIDKPGKGSFCATDLEMILHTRGIRNIVLTGITTDVCVH 174 Query: 116 STVRRALALGF 126 +T+R A GF Sbjct: 175 TTMREANDRGF 185 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.131 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 519,542 Number of extensions: 20815 Number of successful extensions: 115 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 4 Length of query: 176 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 97 Effective length of database: 1,245,805 Effective search space: 120843085 Effective search space used: 120843085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)