BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4460.p (428 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 802 0.0 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 454 e-129 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 148 7e-37 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 125 5e-30 gi|300309866|ref|YP_003773958.1| major facilitator superfamily p... 90 4e-19 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 802 bits (2072), Expect = 0.0, Method: Compositional matrix adjust. Identities = 400/428 (93%), Positives = 413/428 (96%) Query: 1 LKTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI 60 +KTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI Sbjct: 1 MKTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI 60 Query: 61 MQPVCGFVVDLIGLRIGFALFGVLWSLAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM 120 MQPVCGF+VDLIGLRIGFALFGVLWS+AGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM Sbjct: 61 MQPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM 120 Query: 121 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQLRYGWQSAFVVTGVLGFAWAAL 180 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQ+RYGWQSAFVVTG+LGF WAAL Sbjct: 121 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAAL 180 Query: 181 WFAFYRSPHDHAALSEQERTTIVSGQQRPVSAERGKRPVKDVVSSRRFWAIALARFFAEP 240 WFAFYRSP DHAALS+ ER TI+SGQ RPVSA RGKRPVKDVV SRRFWAIALARFFAEP Sbjct: 181 WFAFYRSPRDHAALSDSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEP 240 Query: 241 AWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLVGGYLSPWLINRFRVPLIWS 300 AWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGL GGYLSP+LINRFR+PL+WS Sbjct: 241 AWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWS 300 Query: 301 RVYGVVLGAVMMIGPACVGLVASPYTAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGT 360 RV GVVLGAVMMIGPACVGLVASPY AIALFCVGGFAHQMISGLVNTLSADVFDPEEVGT Sbjct: 301 RVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGT 360 Query: 361 ASGFAGMSAWIGGLGFSLLVGALADKVGYGPLFGMLGAFDLIGAVLLILLIRGQSNEERA 420 ASGFAGMSAWIGGLGFSLLVGALAD VGYGPLFGMLGAFDLIGAVLLI+LIRGQS EERA Sbjct: 361 ASGFAGMSAWIGGLGFSLLVGALADTVGYGPLFGMLGAFDLIGAVLLIILIRGQSTEERA 420 Query: 421 ARTPPRTV 428 AR+ +V Sbjct: 421 ARSLQSSV 428 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 454 bits (1167), Expect = e-129, Method: Compositional matrix adjust. Identities = 219/402 (54%), Positives = 290/402 (72%), Gaps = 3/402 (0%) Query: 1 LKTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI 60 +K ++G+RW+++ LV L I NYLARN+L V APT+ EL MSTQQYSY+V A+Q+ Y + Sbjct: 1 MKYVRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAV 60 Query: 61 MQPVCGFVVDLIGLRIGFALFGVLWSLAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM 120 MQPV G+++D +G +IGF +F + WSL A ATGW SLA FRAL+G+TEAA IP G+ Sbjct: 61 MQPVAGYILDAVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGV 120 Query: 121 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQLRYGWQSAFVVTGVLGFAWAAL 180 KA EWFP KE+SVA+GWFN G+S+GA+ APPLV++ L GW+ +FVV G LG W L Sbjct: 121 KASTEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVL 180 Query: 181 WFAFYRSPHDHAALSEQERTTIVSGQQRPVSAERGKRPV-KDVVSSRRFWAIALARFFAE 239 W FY+SP D LS +ER I+ GQ++ S E+ +R +V SR FW+IA+ RF +E Sbjct: 181 WMLFYKSPRDQKLLSPEERAYILEGQEK--SPEKVQRESWTKIVRSRNFWSIAIPRFLSE 238 Query: 240 PAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLVGGYLSPWLINRFRVPLIW 299 PAWQTF+ WIPLY ERH+++K IAMFAWLPFLAAD+G ++GGYLSP +V L Sbjct: 239 PAWQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFT 298 Query: 300 SRVYGVVLGAVMMIGPACVGLVASPYTAIALFCVGGFAHQMISGLVNTLSADVFDPEEVG 359 SR +++G++ MIGPACVG V SPY AIAL +GGFAHQ +SG + ++++DVF +V Sbjct: 299 SRKLVMLVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVA 358 Query: 360 TASGFAGMSAWIGGLGFSLLVGALADKVGYGPLFGMLGAFDL 401 TA+G GMS ++G F+LL G L ++GYGPLF +L AFDL Sbjct: 359 TATGLTGMSGYLGATLFTLLFGILVTQIGYGPLFVLLAAFDL 400 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 148 bits (374), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 108/397 (27%), Positives = 186/397 (46%), Gaps = 18/397 (4%) Query: 7 LRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCG 66 +R+ I+ L+ + T NY R +L + +K EL + Y+ +AF Y + Q G Sbjct: 11 VRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGG 70 Query: 67 FVVDLIGLRIGFALFGVLWSLAGVLHAGATGWLSLAG-------FRALMGLTEAAAIPSG 119 +++D G + + LWS+ L AG T W+S A R ++GL EA + P+ Sbjct: 71 WLLDRFGSKKIYMWSIALWSVFTFLQAGVT-WMSTAAAVASLFLLRFMVGLAEAPSFPAN 129 Query: 120 MKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQLRYGWQSAFVVTGVLGFAWAA 179 + VA WFP KE+ A FNS A+L PL+ ++ Y W F V GV+G A A Sbjct: 130 GRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALAL 189 Query: 180 LWFAFYRSPHDHAALSEQERTTIVSG------QQRPVSA--ERGKRPVKDVVSSRRFWAI 231 +W SP DH +++ E I SG R V+A +G+ ++ ++S+R + Sbjct: 190 IWGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGV 249 Query: 232 ALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLVGGYLSPWLIN 291 + ++ F W P+Y V ER + + A +P + +GG+VGG +S LI Sbjct: 250 YIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIK 309 Query: 292 RFRVPLIWSRVYGVVLGAVMMIGPACVGLVASPYTAIALFCVGGFAHQMISGLVNTLSAD 351 R + + W+R +V G ++ + V + + + + + F + + L + AD Sbjct: 310 R-GLSVTWARKIPIVGGMLLSMTMILCNYVEAQWMVVGIMALAFFG-KGVGALGWAVVAD 367 Query: 352 VFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADKVG 388 + VG + G M + G+ +++G + + G Sbjct: 368 TAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETG 404 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 125 bits (315), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 86/311 (27%), Positives = 142/311 (45%), Gaps = 15/311 (4%) Query: 15 VCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCGFVVDLIGL 74 + L I NYL R++L + ++ EL +S Q +++AF + Y Q G ++D G Sbjct: 22 LMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIGAMLDKFGA 81 Query: 75 RIGFALFGVLWSLAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVAEWFPDKEKSV 134 R+ +WS+A + R +GL EA P+G K V+EWF +E+ Sbjct: 82 RVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVVSEWFAMRERGG 141 Query: 135 AVGWFNSGTSLGAMLAPPLVIFLQLRYGWQSAFVVTGVLGFAWAALWFAFYRSPHDHAAL 194 G F + +++G LAPP++ L L +GW+ FV+ GVLG A + W+ FYR+ + L Sbjct: 142 PTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRN-RNEVQL 200 Query: 195 SEQERTTIVSGQQRP--VSAERGKRPVKDVVSSRRFWAIALARFFAEPAWQTFSFWIPLY 252 + E + +A+ K + + R W + + W+P Y Sbjct: 201 TPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAY 260 Query: 253 FVNERHLDLKSIAMFAWLPFLAADLGGLVGGYLSPWLINRFRVPLIWSRVYGVVLGAVMM 312 +ERHL + +P++ LG L GY++ L+ R +P I SR + Sbjct: 261 LEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSRKW--------- 310 Query: 313 IGPACVGLVAS 323 P C GL+ + Sbjct: 311 --PICAGLIGA 319 >gi|300309866|ref|YP_003773958.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 484 Score = 89.7 bits (221), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 105/427 (24%), Positives = 167/427 (39%), Gaps = 67/427 (15%) Query: 8 RWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCGF 67 RW ++ L+ L R + G+ P +K E G++ + +V+ F Y ++Q G Sbjct: 43 RWVVMGLIFLIWAIACADRANFGIALPYMKKEYGITNTEAGLIVSLFSFAYGLVQIPVGL 102 Query: 68 VVDLIGLR---IGFALFGVLWSLAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVA 124 + + + I F++F +L SL L + L L +R +GL+E + Sbjct: 103 MYKRLSEKTTGILFSVFMLLTSLFTGLMGTTSSVLLLQLYRVGLGLSEGPLGIGCTNVIN 162 Query: 125 EWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQLRYGWQSAFVVTGVLGFAWAALWFAF 184 WFP +EK A G + + + LG ++ P + I + +GW+ F V V G A LWF Sbjct: 163 RWFPPEEKGTATGLWIAASKLGPLIVPTVCIIVIQLWGWREIFYVFAVPGIFLAILWFFL 222 Query: 185 Y-RSPHDHAALSEQERTTIVSG-QQRPVSAERGKRPVKDVVSSRRFWAIALARFFAEPAW 242 SP ++ +E ER I P +A R + W + R P Sbjct: 223 VTNSPSENRFCNEAERRYITQDVAAGPATATASSRAHVGAQVTPMPWLDGINRTRRVPRL 282 Query: 243 QT--------------------------FSFWIPLYFVNERHLDLKSIAMFAWLPFLAAD 276 +T F WIP Y V + + A PF+ A Sbjct: 283 ETLRQVFTSWNVIGVAIGYGCMIGISNIFMSWIPTYLVTVKGFASVKMGFLASAPFIGAV 342 Query: 277 LGGLVGGYLSPWLINRFRVPLIWSRVYGVVLGAVMMIGPACVGLVASPYTAIALFCVGGF 336 G ++GG +S L+ R P++ G L ++ LV +P + + L Sbjct: 343 AGNMLGGVISDRLLGGRRKPMMLLGALGTALMTLL--------LVEAPDSVLYL-----G 389 Query: 337 AHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADKVGYGPLFGML 396 A M+SGL+ + GFAG SA+ G LA K Y FG++ Sbjct: 390 AMLMLSGLMLGI--------------GFAGYSAYPMG---------LATKATYPAAFGIV 426 Query: 397 GAFDLIG 403 + IG Sbjct: 427 NSLGQIG 433 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,453,122 Number of extensions: 65096 Number of successful extensions: 418 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 387 Number of HSP's successfully gapped: 24 Length of query: 428 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 340 Effective length of database: 1,203,190 Effective search space: 409084600 Effective search space used: 409084600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)