BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4458.p (480 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313868|ref|YP_003777960.1| major facilitator superfamily p... 699 0.0 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 133 3e-32 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 108 1e-24 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 101 1e-22 gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 75 9e-15 >gi|300313868|ref|YP_003777960.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 699 bits (1805), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/476 (83%), Positives = 431/476 (90%), Gaps = 6/476 (1%) Query: 11 VTSSANIPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSLGSVLERSDTLALDAAQ 70 ++++A++PARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGS+GSVLER DTL LDAAQ Sbjct: 3 LSAAASLPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGLDAAQ 62 Query: 71 VGWSGSLYIAGTVLGALLFGRLTDRLGRKRLFMLTLALYMAATLATAFAQDFLWLAVCRL 130 VGW+GSLYIAG V+GAL+FGRLTDRLGRK+LFM+TLALYMAATLA+AFA DFLW A+CRL Sbjct: 63 VGWTGSLYIAGAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRL 122 Query: 131 VTGLGIGGEYAAINSAIDELVPARVRGRVNLIINASFWIGAALGAGLSLLLLDPQVLGPA 190 TGLGIGGEYAAINSAIDELVPARVRGRVNL+INASFWIGAALGAGLSL++LD +VLGP Sbjct: 123 ATGLGIGGEYAAINSAIDELVPARVRGRVNLVINASFWIGAALGAGLSLVILDERVLGPV 182 Query: 191 LGWRACFLLGAVLAVAILLVRRHVPESPRWLLMHGRHAEAEAVVRHIEAEVAAQHGALPA 250 +GWR CFLLGAVLAVAI+LVRRHVPESPRWL MHG HA AEAVVR IEA+V A+HG LPA Sbjct: 183 MGWRVCFLLGAVLAVAIVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQVMAEHGTLPA 242 Query: 251 TP------DSGHPQPGGGMMAWREIAQVLLHRYRQRSLMVALMMVAQAFVYNAIFFTYSL 304 P S P G+++WREIAQVLLH YR RSLMVALMMVAQAFVYNAIFFTYSL Sbjct: 243 LPTAAPGESSRSPGQAVGLLSWREIAQVLLHHYRARSLMVALMMVAQAFVYNAIFFTYSL 302 Query: 305 VLTRFFNVPDHRVALYIFPFALGNVLGPLLLGQLFDTIGRRRMIAATYLLAGAGLAATAW 364 VLTRFF VPD RVALY+FPFALGNVLGPLLLG LFD+IGRRRMIAATYLLAG GLAATA Sbjct: 303 VLTRFFQVPDTRVALYLFPFALGNVLGPLLLGHLFDSIGRRRMIAATYLLAGVGLAATAA 362 Query: 365 AFNAGWLDARTLALCWSAVFFVASAAASSAYLTASEVFPLRMRAMAISIFYAIGTGVGGF 424 AFNAGWLDA LALCWSAVFFVASAAASSAYLTASEVFPL+MRAMAI+IFYAIGTGVGGF Sbjct: 363 AFNAGWLDALGLALCWSAVFFVASAAASSAYLTASEVFPLQMRAMAIAIFYAIGTGVGGF 422 Query: 425 VAPVLLGWLIASGDRHAVAAGYALGAGLALLAGGCAWRWAVDAERRTLEDVAEWKN 480 +AP LLGWLIASGDR AVAAGYA+GA LALLAG CA RWAVDAERRTLE+VAE K Sbjct: 423 IAPALLGWLIASGDRQAVAAGYAVGAVLALLAGLCALRWAVDAERRTLEEVAELKR 478 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 133 bits (335), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 144/500 (28%), Positives = 228/500 (45%), Gaps = 73/500 (14%) Query: 8 SSSVTSSANIPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSLGSVLERSDTL-AL 66 S +A + R++ +P+SRWH + I +G A + D SL VL L L Sbjct: 20 SDDAHRAAGLLFRIENVPFSRWHTKARIIMGSATLFDAFHAL---SLAFVLPVLVGLWQL 76 Query: 67 DAAQVGWSGSLYIAGTVLGALLFGRLTDRLGRKRLFMLTLALYMAATLATAFAQDFLWLA 126 AAQ+G + G V+GAL FG + +R GR +T+ L ++ A F L Sbjct: 77 SAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLF 136 Query: 127 VCRLVTGLGIGGEYAAINSAIDELVPARVRGRVNLIINASFWIGAALGAGLSLLLLDPQV 186 +CR + G+G+GGE + I+EL A RG+ ++ F +G A + L+ Sbjct: 137 LCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLV---- 192 Query: 187 LGPALGWRACFLLGAVLAVAILLVRRHVPESPRWLLMHGRHAEAEAVVRHIEAEVAAQHG 246 P GW FL+G + + + L+ +PESPRWL+ GR +AE ++R +EA ++ Sbjct: 193 --PRFGWEYMFLVGGIPGILVALLITRLPESPRWLIARGRLDDAEKLIRTLEASTPKRN- 249 Query: 247 ALPATPD--------------SGHPQPGGGMMAWREIAQVLLHRYRQRSLMVALMMVAQA 292 L A D +G Q G +W+E+ + YR R+++V L+ + Sbjct: 250 -LDAQTDKQAIEQRINALAAGAGKRQRG----SWKELFSPV---YRGRTIIVWLLWASSY 301 Query: 293 FVYNAIFFTYSLVLTRFFNVPDH---RVALYIFPFALGNVLGPLLLGQLFDTIGRRRMIA 349 FV N I + +++P R+A + VLG L L D +GRRR Sbjct: 302 FVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCAL---LIDRVGRRRWAI 358 Query: 350 ATYLLAGAGLAATAWAFNAGWLDARTLALCWSAVFFVASAAASSA------YLTASEVFP 403 A+++L+GA LA WS +F +SA A YL E++P Sbjct: 359 ASFILSGALLALLG---------VLKAESAWSVMFLASSAYAVMGTTTVLLYLYTPEIYP 409 Query: 404 LRMRAMAISIFYAIGTGVGG-------FVAPVLLGWLIASGDRHAVAAGYALGAGLALLA 456 RMR AIGTG+ AP ++G ++A H +A + + AG+ +L Sbjct: 410 TRMR--------AIGTGLATSWLRAASAAAPAMVGVVLAG---HGIATVFLMFAGVTVLG 458 Query: 457 GGCAWRWAVDAERRTLEDVA 476 AWR ++ R+LE+++ Sbjct: 459 LLAAWR-MIETTNRSLEEIS 477 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 70/233 (30%), Positives = 120/233 (51%), Gaps = 8/233 (3%) Query: 28 RWHWRVVIALGVAWVLDGLEVTLVGSLGSVLERSDTLALDAAQVGWSGSLYIAGTVLGAL 87 ++ +R+ + G+ W+ D ++V +G + + T L Q +G+++ G +LGA Sbjct: 19 KFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAK--TFGLTVPQALQTGTMFFVGMLLGAF 76 Query: 88 LFGRLTDRLGRKRLFMLTLALYMAATLATAFAQDFLWLAVCRLVTGLGIGGEYAAINSAI 147 +FGRL DR+GR+ + M + + +A+AFA DF WL RL+TG+G+GG + + Sbjct: 77 VFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMM 136 Query: 148 DELVPARVRGRVNLIINASFW-IGAALGAGLSLLLLDPQVLGPALGWRACFLLGAVLAVA 206 E +P+ RGR L++ SFW IG A L+L+ V WR F + + A+ Sbjct: 137 AEFLPSARRGR-WLVMLESFWAIGTICLAVLALV----AVSWGNDAWRVIFFVTGLPALV 191 Query: 207 ILLVRRHVPESPRWLLMHGRHAEAEAVVRHIEAEVAAQHGALPATPDSGHPQP 259 +++R +VPESP +L G+ A V+ + + P P+ +P Sbjct: 192 GVVLRFYVPESPMYLNRSGKSDAARQVLERVAKVNGSSTPIPPLQPEVVERKP 244 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 64/228 (28%), Positives = 116/228 (50%), Gaps = 8/228 (3%) Query: 13 SSANIPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSLGSVLERSDTLALDAAQVG 72 + ++ +D ++++HW V+ + V DG ++ + G++ VL + +L Q G Sbjct: 2 KTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKE--WSLSPVQAG 59 Query: 73 WSGSLYIAGTVLGALLFGRLTDRLGRKRLFMLTLALYMAATLATAFAQDFLWLAVCRLVT 132 GS + G + GA +FG L DR+GRK+ ++ L+ +T + FA V R + Sbjct: 60 ALGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLA 119 Query: 133 GLGIGGEYAAINSAIDELVPARVRGRVNLIINASFWIGAALGAGLSLLLLDPQVLGPALG 192 GLG GG + ++E P R+R + I+ + + +G L AGL + +L P G Sbjct: 120 GLGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWML------PNFG 173 Query: 193 WRACFLLGAVLAVAILLVRRHVPESPRWLLMHGRHAEAEAVVRHIEAE 240 W++ F AV V + ++ +PES +LL GR +A A++ ++ + Sbjct: 174 WQSMFFAAAVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQ 221 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 75.5 bits (184), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 101/400 (25%), Positives = 160/400 (40%), Gaps = 59/400 (14%) Query: 65 ALDAAQVGWSGSLYIAGTVLGALLFGRLTDRLGRKRLFMLTLALYMAATLATAFAQDFLW 124 ++ A+ G+ + + + +G G L DR GR R+ T+ + T + F F Sbjct: 43 SMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQ 102 Query: 125 LAVCRLVTGLGIGGEYAAINSAIDELVPARVRGRVNLIINASFWIGAALGAGLSLLLLDP 184 L R + G G+GGE+A + I E + AR RG+ + ++ +S+ +G A G+ L+ + Sbjct: 103 LFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQSSWAVGWA-GSALAFWGVYA 161 Query: 185 QVLGPALGWRACFLLGAVLAVAILLVRRHVPESPRWLLMHGRHAEAEAVVRHIEAEVAAQ 244 VL P W+ F +G + A+ L +RR + E +L R E + Q Sbjct: 162 -VLPPETAWKVLFWIGVLPALLTLYIRRAISEPAVYLATQARLRE------------SGQ 208 Query: 245 HGALPATPDSGHPQPGGGMMAWREIAQVLLHRYRQRSLMVALMMVAQAFVYNAIFFTYSL 304 G + HR R+ ++A ++ +++ + Sbjct: 209 RGNFLR----------------------IFHRDLLRTTLLASLLATGM---QGAYYSVTT 243 Query: 305 VLTRFFNVPDHRVAL----YIFPFALGNVLGPLLLGQLFDTIGRRRMIAATYLLAGAGLA 360 L F H L Y+ G+ G L L DTIGRRR L A G+ Sbjct: 244 WLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFM---LFAAMGIV 300 Query: 361 ATAWAFNAGWLDARTLALCWSAVFFVASA-AASSAYLTASEVFPLRMRAMAISIFYAIGT 419 DA LAL + FF++ + AYL SE+FP +R Y G Sbjct: 301 LVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYL--SELFPSDVRGSGQGFCYNFGR 358 Query: 420 GVGGFVAPVLLGWLIASGDRHAVAAGYALGAGLALLAGGC 459 VG + P L+G+L S LG + +A GC Sbjct: 359 AVGA-ICPALVGYLSNS---------LPLGQTIGWIAAGC 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,245,771 Number of extensions: 45167 Number of successful extensions: 416 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 401 Number of HSP's successfully gapped: 23 Length of query: 480 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 391 Effective length of database: 1,198,455 Effective search space: 468595905 Effective search space used: 468595905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)