BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4456.p (487 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 816 0.0 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 263 3e-71 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 258 6e-70 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 253 3e-68 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 248 6e-67 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 816 bits (2108), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/487 (84%), Positives = 449/487 (92%) Query: 1 MPSTPFLDSACWQRHLFRGRWEPAAQGATVIEPATGQPLTEVGLATPQDVDISVQAAVAA 60 M ++PFLDSA WQ+HLFRG+W AAQGATV+EPATG+PLT+VGLA+P DV SV+ A+AA Sbjct: 1 MQASPFLDSAQWQQHLFRGQWVAAAQGATVLEPATGRPLTQVGLASPDDVSASVELAIAA 60 Query: 61 QAEWQAVPPRERADLFRRAGALFQQHFDELARMVARETGGVLFKGEHEVREAIILCHLAA 120 QA W A+PPRERAD+FRRA LF QHFDELARMVARETGGVLFKGEHEVREAI LCHLAA Sbjct: 61 QAAWVAMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAA 120 Query: 121 AMPLQAQGQVLPSVPGRLSLARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKP 180 MPLQAQGQ+LPS PGRLS+ARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKP Sbjct: 121 GMPLQAQGQLLPSTPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKP 180 Query: 181 DARTPVSGGFMLARVFEEAGLPAGLLHVLPGGAEAGEALVSAPQVPMIAFTGSPAVGRRI 240 D RTPVSGGFMLAR+FE+AGLPAGLLHVLPGGAE GEALV+AP VPMIAFTGSPAVGRRI Sbjct: 181 DLRTPVSGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRRI 240 Query: 241 GELAGRHLKKVSLELGGANALVILEDADLDVAASNAAWGAWLHQGQICMAANRILVHRSQ 300 GELAGRHLKK+SLELGGANALVILEDADLDVAAS+AAWGAWLHQGQICMAANRILVH S Sbjct: 241 GELAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASL 300 Query: 301 APGLLERLVEKARHLPVGDGASGQVALGPLIDQRQLQRVHGLVQDSVAAGARLLAGGTYE 360 GLL+RL +KARHLPVGDGASGQVALGP+ID+RQLQRVH LVQ+SVAAGA+L+AGG YE Sbjct: 301 EEGLLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYE 360 Query: 361 GLFYQPTVLADVKPGMRVFDEEIFGPVASVISYDSQEQAIALANQNQGGLAAGVISRSVG 420 LFYQPTVL V+PGMRVF+EEIFGPVASVI +D+ E AIALAN++ GGLAAGV+S SVG Sbjct: 361 QLFYQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVG 420 Query: 421 RAMAVAAQLRQGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWKWITVKQ 480 RAMAVAA+LRQGMVHVNDQTVNDECVNPFGGPGIAGNG SVGGPADWEEYTQW+W T++Q Sbjct: 421 RAMAVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQ 480 Query: 481 EASAYPF 487 +A YPF Sbjct: 481 QAMPYPF 487 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 263 bits (671), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 168/424 (39%), Positives = 236/424 (55%), Gaps = 7/424 (1%) Query: 69 PRERADLFRRAGALFQQHFDELARMVARETGGVLFKGEHEVREAIILCHLAAAMPLQAQG 128 P +R L A Q D+ A+ +A ETG V+ A AAAM Q G Sbjct: 62 PGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAG 121 Query: 129 QVLPS-VPGRLSLARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDARTPVS 187 +V+PS VPG L++ R P GV+ I+P+N P+IL +R +A LA GN VV K P + Sbjct: 122 EVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPAT 181 Query: 188 GGFMLARVFEEAGLPAGLLHVLPGGAE----AGEALVSAPQVPMIAFTGSPAVGRRIGEL 243 G ++A +EAGLP G+++ + E EA+++ P V + FTGS VGR I Sbjct: 182 HGLIIA-ALQEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQ 240 Query: 244 AGRHLKKVSLELGGANALVILEDADLDVAASNAAWGAWLHQGQICMAANRILVHRSQAPG 303 RHLK LELGG ++L DADLD A AA+GA+ + GQICM+ RI+V S A Sbjct: 241 CARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADE 300 Query: 304 LLERLVEKARHLPVGDGASGQVALGPLIDQRQLQRVHGLVQDSVAAGARLLAGGTYEGLF 363 + RL KA LP+GD G V LG ++D ++R + L+ D++A GA L+ GG Sbjct: 301 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTL 360 Query: 364 YQPTVLADVKPGMRVFDEEIFGPVASVISYDSQEQAIALANQNQGGLAAGVISRSVGRAM 423 T+L V M ++ EE FGPV ++ E AIA AN N+ GL++ + SR V RA+ Sbjct: 361 MPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARAL 420 Query: 424 AVAAQLRQGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWKWITVKQEAS 483 A++ ++ G+ H+N TV+DE PFGG +G G GG A E+T+ +W+TV+ Sbjct: 421 ALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFG-HFGGKAGIAEFTELRWVTVQTTPR 479 Query: 484 AYPF 487 YPF Sbjct: 480 HYPF 483 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 258 bits (660), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 168/453 (37%), Positives = 240/453 (52%), Gaps = 9/453 (1%) Query: 13 QRHLFRGRWEPAAQGA--TVIEPATGQPLTEVGLATPQDVDISVQAAVAAQAEWQAVPPR 70 Q+ G+W A QG V PA G+ L V L + +++AA AA W+ + Sbjct: 12 QQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAK 71 Query: 71 ERADLFRRAGALFQQHFDELARMVARETGGVLFKGEHEVREAIILCHLAAAMPLQAQGQV 130 ER+ + RR L + D+LA ++ E G L + E+ A A + G Sbjct: 72 ERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDT 131 Query: 131 LPS-VPGRLSLARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDARTPVSGG 189 +PS P + + +GV I+P+NFP + R PALAAG +V+KP TP S Sbjct: 132 IPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSA- 190 Query: 190 FMLARVFEEAGLPAGLLHVLPGGAEA-GEALVSAPQVPMIAFTGSPAVGRRIGELAGRHL 248 LA + E AG+PAG+ V+ G + G + S P V I+FTGS VG+ + + + + Sbjct: 191 LALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSI 250 Query: 249 KKVSLELGGANALVILEDADLDVAASNAAWGAWLHQGQICMAANRILVHRSQAPGLLERL 308 KK+SLELGG ++ +DADLD A A + + GQ C+ ANRI V +L Sbjct: 251 KKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKL 310 Query: 309 VEKARHLPVGDGASGQVALGPLIDQRQLQRVHGLVQDSVAAGARLLAGGTYEGL---FYQ 365 VE + VG G V GPLI+++ +Q+V V D+VA GAR+L GG L F++ Sbjct: 311 VEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFE 370 Query: 366 PTVLADVKPGMRVFDEEIFGPVASVISYDSQEQAIALANQNQGGLAAGVISRSVGRAMAV 425 PTVLADV P M+V EE FGP+A + + + E+ +ALAN + GLA+ SR +GR V Sbjct: 371 PTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRV 430 Query: 426 AAQLRQGMVHVNDQTVNDECVNPFGGPGIAGNG 458 A L GMV +N +++E V PFGG +G G Sbjct: 431 AEGLESGMVGINTGLISNE-VAPFGGVKQSGLG 462 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 253 bits (645), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 155/469 (33%), Positives = 264/469 (56%), Gaps = 10/469 (2%) Query: 16 LFRGRWEPAAQGAT--VIEPATGQPLTEVGLATPQDVDISVQAAVAAQAEWQAVPPRERA 73 G W +A G T VI PAT + + ++ A D+D +++AA W+ + ER+ Sbjct: 8 FINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERS 67 Query: 74 DLFRRAGALFQQHFDELARMVARETGGVLFKGEHEVREAIILCHLAAAMPLQAQGQVLPS 133 + RRA L ++ +E+AR++ E G L + + E A A + G+++P+ Sbjct: 68 KIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPA 127 Query: 134 -VPGRLSLARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDARTPVSGGFML 192 VPG + + P+G + +P+NFPL +R ++ ALAAG +++VK TP S ++ Sbjct: 128 RVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELI 187 Query: 193 ARVFEEAGLPAGLLHVLPG-GAEAGEALVSAPQVPMIAFTGSPAVGRRIGELAGRHLKKV 251 R + +AG+PAG+++++ G AE E L+ P + I+FTGS VG+++ LAG+H+K+ Sbjct: 188 -RAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRA 246 Query: 252 SLELGGANALVILEDADLDVAASNAAWGAWLHQGQICMAANRILVHRSQAPGLLERLVEK 311 ++ELGG ++ +DAD+DVAA A + + GQ+C++ R LV + +++ V Sbjct: 247 TMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTY 306 Query: 312 ARHLPVGDGASGQVALGPLIDQRQLQRVHGLVQDSVAAGARLLAGGT---YEGLFYQPTV 368 A L VG+G + +GP+ ++R++ + L+ D+V+ GA+L GG +G F+QPTV Sbjct: 307 AEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTV 366 Query: 369 LADVKPGMRVFDEEIFGPVASVISYDSQEQAIALANQNQGGLAAGVISRSVGRAMAVAAQ 428 L ++ R +EE FGP+A + S+++ ++ I AN+ GLAA ++S A+AA+ Sbjct: 367 LTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAAR 426 Query: 429 LRQGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWKWIT 477 + GM+ +N + + PFGG +G G S GG E Y K +T Sbjct: 427 VESGMLTINHLGLALPEL-PFGGIKDSGYG-SEGGTEAIEAYINTKLVT 473 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 248 bits (634), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 170/424 (40%), Positives = 239/424 (56%), Gaps = 12/424 (2%) Query: 64 WQAVPPRERADLFRRAGALFQQHFDELARMVARETGGVLFKGEH-EVREAIILCHLAAAM 122 W + +R L + AL ++ D LA M AR G + +V AI AA Sbjct: 60 WSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGW 119 Query: 123 PLQAQGQVLPSV----PGRLSLARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVV 178 Q +G+ +P+ LS R P+GV+G I P+N PL++T VA LAAG VV+ Sbjct: 120 ANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVI 179 Query: 179 KPDARTPVSGGFMLARVFEEAGLPAGLLHVLPG-GAEAGEALVSAPQVPMIAFTGSPAVG 237 KP TP S LAR+ +EAG P G+++V+ G G G AL P V I+FTGSP G Sbjct: 180 KPAEETPQSA-LHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAG 238 Query: 238 RRIGELAGRHLKKVSLELGGANALVILEDADLDVAASNAAWGAWLHQGQICMAANRILVH 297 R I +AG K+V+LELGG + ++ +DA + A G +++QGQ+C A +RILV Sbjct: 239 RAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQ 298 Query: 298 RSQAPGLLERLVEKARHLPVGDGASGQVALGPLIDQRQLQRVHGLVQDSVAAGARLLAGG 357 RS A + L E A + VGD + V +GP+ + Q +RV+ +Q + GA LLAGG Sbjct: 299 RSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG 358 Query: 358 TY---EGLFYQPTVLADVKPGMRVFDEEIFGPVASVISYDSQEQAIALANQNQGGLAAGV 414 +G F QPT+ AD GM + EEIFGPV ++IS+D++E+AIALAN ++ GLAA V Sbjct: 359 VSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATV 418 Query: 415 ISRSVGRAMAVAAQLRQGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWK 474 + ++ RA VAA ++ G V VN + D + P+GG +G G GG A YT+ K Sbjct: 419 WTTNLARAHRVAAGVKVGAVGVNCWSPLDANL-PWGGIKDSGMGRE-GGLAGALAYTEEK 476 Query: 475 WITV 478 +TV Sbjct: 477 TVTV 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,761,038 Number of extensions: 84039 Number of successful extensions: 390 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 319 Number of HSP's successfully gapped: 21 Length of query: 487 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 398 Effective length of database: 1,198,455 Effective search space: 476985090 Effective search space used: 476985090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)