BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4455.p (255 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313865|ref|YP_003777957.1| 3-alpha-hydroxysteroid dehydrog... 444 e-126 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 78 7e-16 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 66 3e-12 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 63 2e-11 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 58 6e-10 >gi|300313865|ref|YP_003777957.1| 3-alpha-hydroxysteroid dehydrogenase/carbonyl reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 444 bits (1142), Expect = e-126, Method: Compositional matrix adjust. Identities = 212/255 (83%), Positives = 228/255 (89%) Query: 1 MKLQDKTIVVTGVSSGIGAETARLLRVQGARVIGVDRNAPMISMEGFVQADLSTVEAIDA 60 M LQDKT+VVTGVSSGIGAETAR LR QGARVIGVDRN P IS+E FVQADLS+ EAIDA Sbjct: 1 MNLQDKTVVVTGVSSGIGAETARQLRAQGARVIGVDRNEPAISVETFVQADLSSAEAIDA 60 Query: 61 AVAQLPAQVDGLCNVAGVPGTAPVDLVGRVNYLGLRHLTERLIARMAPGSAIVNVASILG 120 VAQLP ++D LCN+AGVPGTA LV RVNYLGLRHL +RL AR++PG AIVNVASILG Sbjct: 61 MVAQLPERIDALCNIAGVPGTAEAQLVARVNYLGLRHLVQRLTARLSPGGAIVNVASILG 120 Query: 121 AEWPKRLEPHKALAQTASFAAGADWLAQHPVAQESCYQYFKEALIVWTLTRAQPLFLRQS 180 AEWP+RLE HKAL+Q ASFA GA WLAQHPVAQE CYQYFKEALIVW++T+AQP+FL+ S Sbjct: 121 AEWPQRLEQHKALSQAASFAEGAAWLAQHPVAQEHCYQYFKEALIVWSMTQAQPMFLQHS 180 Query: 181 VRMNSVAPGPVLTPILGDFVTMLGQERVQKDAHRMKRPAYPDEIAAAIVWLCGDDSRWIN 240 VRMN VAPGPV TPILGDFVTMLGQERVQKDAHRMKRPAY DEIAAAIVWLC DDSRWIN Sbjct: 181 VRMNCVAPGPVFTPILGDFVTMLGQERVQKDAHRMKRPAYADEIAAAIVWLCSDDSRWIN 240 Query: 241 GVNLPVDGGLASTYL 255 GVNLPVDGGLASTYL Sbjct: 241 GVNLPVDGGLASTYL 255 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 77.8 bits (190), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 88/280 (31%), Positives = 118/280 (42%), Gaps = 63/280 (22%) Query: 2 KLQDKTIVVTGVSSGIGAETARLLRVQGARVIGVDRNAPMI-------------SMEGFV 48 + + K ++VTG +SGIG TAR +GARV+ DR+A + + E V Sbjct: 11 RFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETDV 70 Query: 49 QADLSTVEAIDAAVAQLPAQVDGLCNVAGVPGTAPVDLVG----------RVN--YLGLR 96 + +D A+ + Q+D L + AGV V V VN + G R Sbjct: 71 SHHEQVRQLVDFAIERF-GQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAR 129 Query: 97 HLTERLIARMAPGSAIVNVASILGAEWPKRLEPHKALAQTASFAAGADWLAQHPVAQESC 156 L IVNVAS+ G ADW S Sbjct: 130 EALPHLEKTRG---CIVNVASVSGL--------------------AADW-------NLSA 159 Query: 157 YQYFKEALIVWTLTRAQPL-FLRQSVRMNSVAPGPVLTPILGDFVTMLGQERVQKDAHR- 214 Y K A V LTRA L F R+ VR+N+V P T + D + K A R Sbjct: 160 YNASKGA--VCNLTRAMALDFGRKGVRINAVCPSLTHTAMTADMAD--DPPLLDKFAERI 215 Query: 215 -MKRPAYPDEIAAAIVWLCGDDSRWINGVNLPVDGGLAST 253 + R A P EIAA I +L D+ ++NGVNLPVDGGL ++ Sbjct: 216 ALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGGLMAS 255 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 78/264 (29%), Positives = 120/264 (45%), Gaps = 43/264 (16%) Query: 6 KTIVVTGVSSGIGAETARLLRVQGARVIGVDRN--APMISMEGFV---------QADLST 54 + +VTG + GIGA ++ L +G V+ D N A + + V Q DL + Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 55 VEAIDAAVAQLP---AQVDGLCNVAGVPGTAPVDLVGRVNYLGLRHLTERLIARMAPGSA 111 E+I AA A + + D L N AGV T Y L + E + M Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVART----------YSFLDYPLENWLQTMN---- 114 Query: 112 IVNVASIL-GAEWPKRLEPHKALAQTASFAAGADWLAQHPVAQESCYQYFKEALIVWTLT 170 VNV +L + RL + + + ++ + A A + Y K A+I LT Sbjct: 115 -VNVTGVLLAGQHAARLMVKQQWGRIVNISSISGIRAG---AGRTAYGTSKAAVI--GLT 168 Query: 171 RAQPLFLRQ-SVRMNSVAPGPVLTPILGDFVTMLGQERVQKDAHR---MKRPAYPDEIAA 226 R + L Q + +NSVAPGPV TP+ + E+ ++ +R M+R DEIA+ Sbjct: 169 RQMAIELAQYGITVNSVAPGPVDTPM----TQAMHSEQTRESYYRLVPMRRYGTTDEIAS 224 Query: 227 AIVWLCGDDSRWINGVNLPVDGGL 250 A+ +L ++S +I G +PVDGG Sbjct: 225 AVCYLAAEESAYITGHVIPVDGGF 248 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 63.2 bits (152), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 75/284 (26%), Positives = 119/284 (41%), Gaps = 74/284 (26%) Query: 2 KLQDKTIVVTGVSSGIGAETARLLRVQGARVIGVDRNAPMISMEGF--------VQADLS 53 +L K +VTG +SGIG TA+ L QGA+V R + ++AD S Sbjct: 4 QLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRADAS 63 Query: 54 TVEAIDAAVAQLP---AQVDGLCNVAGVPGTAPV--------DLVGRVNYLGLRHLTERL 102 + +D AQ+ ++D L AG P+ D + N G+ ++ Sbjct: 64 VLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTVQKA 123 Query: 103 IARMAPGSAIV---NVASILGAEWPKRLEPHKALAQTASFAAGADWLAQHPVAQESCYQY 159 + + G++++ + S+LG TA+F S Y Sbjct: 124 LPLLVDGASVILTSSTTSVLG---------------TANF---------------SVYSA 153 Query: 160 FKEALIVWTLTRAQPLFLR-QSVRMNSVAPGPVLTPILGDFVTMLGQERVQKDAHR---- 214 K A V R+ L L+ +++R+N V+PGP+ TP LG E V DA + Sbjct: 154 SKAA--VRNFARSWALDLKDRAIRVNVVSPGPIRTPGLG--------ELVPDDARQGLFD 203 Query: 215 -------MKRPAYPDEIAAAIVWLCGDDSRWINGVNLPVDGGLA 251 + R P EI A+ +L D + ++NG+ L VDGG+A Sbjct: 204 YLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGIELFVDGGMA 247 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 58.2 bits (139), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 77/271 (28%), Positives = 116/271 (42%), Gaps = 49/271 (18%) Query: 3 LQDKTIVVTGVSSGIGAETARLLRVQGARVIGVDRNAP--------MISMEG----FVQA 50 L K V G S GIGA + L +GA V ++P + +G +QA Sbjct: 15 LVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQA 74 Query: 51 DLSTVEAIDAAV---AQLPAQVDGLCNVAGVPGTAPVDLVGRVNYLGLRHLTERLIARMA 107 D + A+ A+ A+ ++D L N AGV P+D L L +R +A Sbjct: 75 DSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLD------QFKLEDL-DRTLA--- 124 Query: 108 PGSAIVNVASILGAEWPKRLEPHKALAQTAS-FAAGADWLAQHPVAQESCYQYFKEALIV 166 VNV S+ A E + + + + G+ + P A + Y K AL+ Sbjct: 125 -----VNVRSVFVATQ----EAARYMGEGGRVISIGSTNADRIPFAGGAVYAMSKSALVG 175 Query: 167 WTLTRAQPLFLRQSVRMNSVAPGPV---LTPILGDFVTMLGQERVQKDAHRMKRPAY--P 221 T A+ L R + +N+V PGPV + P GDF L M P Y Sbjct: 176 LTKGLARDLGPR-GITVNNVQPGPVDTDMNPASGDFAASL--------TGLMALPRYGKA 226 Query: 222 DEIAAAIVWLCGDDSRWINGVNLPVDGGLAS 252 +EIA+ + +L G +S +I G NL +DGG ++ Sbjct: 227 EEIASFVAYLAGPESGYITGANLMIDGGFSA 257 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 871,852 Number of extensions: 37874 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 252 Number of HSP's successfully gapped: 9 Length of query: 255 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 172 Effective length of database: 1,226,865 Effective search space: 211020780 Effective search space used: 211020780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)