BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4454.p (322 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313864|ref|YP_003777956.1| enzyme involved in meta-pathway... 565 e-162 gi|300312311|ref|YP_003776403.1| enzyme involved in meta-pathway... 69 6e-13 gi|300310336|ref|YP_003774428.1| hypothetical protein Hsero_1003... 59 4e-10 >gi|300313864|ref|YP_003777956.1| enzyme involved in meta-pathway of phenol degradation protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 565 bits (1456), Expect = e-162, Method: Compositional matrix adjust. Identities = 266/294 (90%), Positives = 285/294 (96%) Query: 29 FDQPSVNMGGTSFFDGAPLPGGPGFYFIEYLSYTKATKLVDNNGNRMGFPSTQDISVFVP 88 +DQP+VNMGGTSFFDGAPLPGGPGFYF+EYL+Y+KATKLVDNNGNR+GFP+TQDISVF P Sbjct: 23 YDQPTVNMGGTSFFDGAPLPGGPGFYFVEYLTYSKATKLVDNNGNRLGFPATQDISVFAP 82 Query: 89 TSQFIFVPQGAKFGNKQLGFQVLVPWVARAKVDDGANNAILRGNTGIGDISAGVSVQYDP 148 +QFI+VP GAKFGNKQLGFQVLVPWVA AKVDDGANNA+LRGNTGIGDISAGVSVQYDP Sbjct: 83 VTQFIYVPPGAKFGNKQLGFQVLVPWVASAKVDDGANNAVLRGNTGIGDISAGVSVQYDP 142 Query: 149 IMGKQGPLYAQRFELQFILPTGAYDRNATVNPGSNFWSFDPYWAGTLWASENLSFSWRLH 208 IMG QGPLYAQRFELQFILPTGAYDRNATVNPGSN W+FDPYWAGTLWAS+NLSFSWRLH Sbjct: 143 IMGAQGPLYAQRFELQFILPTGAYDRNATVNPGSNTWAFDPYWAGTLWASQNLSFSWRLH 202 Query: 209 YLWNARNNSPSAAFGNNVTSTQAGQAVHANFAAEYVLNPTWTVGASGYWLQQISDTQVNG 268 YLWNARNN+PSAAFGNNV+STQAGQA HANFAAEYVLNPTWTVGA+GYWLQQ++DT+VNG Sbjct: 203 YLWNARNNAPSAAFGNNVSSTQAGQAAHANFAAEYVLNPTWTVGAAGYWLQQVTDTKVNG 262 Query: 269 ADVSGRRERVVGLGPAAMMRLSDRDSLFFNVYQEFAVRNRAENSKIQIRYDHHF 322 ADVSGRRERVVGLGPAAMMRLS+RD LFFNVYQEFAVRNRAENSK QIRYDHHF Sbjct: 263 ADVSGRRERVVGLGPAAMMRLSERDLLFFNVYQEFAVRNRAENSKFQIRYDHHF 316 >gi|300312311|ref|YP_003776403.1| enzyme involved in meta-pathway of phenol degradation protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 68.6 bits (166), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 49/160 (30%), Positives = 68/160 (42%), Gaps = 11/160 (6%) Query: 163 LQFILPTGAYDRNATVNPGSNFWSFDPYWAGTLWASENLSFSWRLHYLWNARNNSPSAAF 222 L PTGAY + N G N W++ P+ T S L S ++ N N + Sbjct: 168 LMIQAPTGAYSTSKAFNAGVNHWTYSPFVGATYITSSGLELSTQVSLNVNTVNPA----- 222 Query: 223 GNNVTSTQAGQAVHANFAAEYVLNPTWTVGASGYWLQQISDTQVNGADVSGRRERVVGLG 282 T ++G FA + +WT G +GY +Q+SD G+ G R RV LG Sbjct: 223 ----TRYRSGVEYRQEFAIGQHIQ-SWTAGLAGYVYRQLSDDSGPGSG-DGNRGRVYALG 276 Query: 283 PAAMMRLSDRDSLFFNVYQEFAVRNRAENSKIQIRYDHHF 322 PA + + YQEF RNRAE + +R F Sbjct: 277 PAVSFAQPGLPLVTLHGYQEFGARNRAEGYNLALRLAMTF 316 >gi|300310336|ref|YP_003774428.1| hypothetical protein Hsero_1003 [Herbaspirillum seropedicae SmR1] Length = 305 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 86/331 (25%), Positives = 139/331 (41%), Gaps = 49/331 (14%) Query: 1 MRKIKRHARRIGATALLAATLCPGLASAFDQPSVNMGGTS-FFDG------APLPGGPGF 53 M++ A R+ ALL P A AF+ GG S F G A LP PG Sbjct: 1 MKRGFVPACRLATCALLTMAAGPA-AYAFE------GGVSPFLTGSTSEYIAALPPIPGL 53 Query: 54 YFIEYLSYTKATKLVDNNGNRMGFPSTQDISVFVPTSQFIFVPQGAKFGNKQLGF----Q 109 + +E +Y+ + L DNNGN++ P S + T++ + A +G + LG Q Sbjct: 54 FAVEQFNYSSSNGLYDNNGNKLPIPFKS--SAYSATTRLL-----ASYGVQWLGAKVYSQ 106 Query: 110 VLVPWVARAKVDDGANNAILRGNTGIGDISAG-VSVQYDPIMGKQGPLYAQRFELQFILP 168 +++P V+ G + + G+ +++A V +++D F L Sbjct: 107 LVLPVVSLHTEVAGHSET----HNGLSNVTATPVLLRWDVAAHTN-----VTFGFDISLE 157 Query: 169 TGAYD-RNATVNPGSNFWSFDPYWAGTLWASENLSFSWRLHYLWNARNNSPSAAFGNNVT 227 TG+Y +V G W P A + L L N RN+S T Sbjct: 158 TGSYSPTRPSVAVGYTSW--QPVLAVRHNNPDGLDLGISNRLLINDRNSS---------T 206 Query: 228 STQAGQAVHANFAAEYVLNPTWTVGASGYWLQQISDTQVNGADVSGRRERVVGLGPAAMM 287 + ++G A A+F + + W G +G +L Q +D + NGAD++G R R + LGP Sbjct: 207 NYRSGTAYVADFIGGWNVG-KWKFGVTGSYLNQFTDDRQNGADITGNRARSLSLGPTVAY 265 Query: 288 RLSDRDSLFFNVYQEFAVRNRAENSKIQIRY 318 + S+ N + N A++ I I + Sbjct: 266 N-AGPFSINMNYQRGLYAANTAKSDAIWINF 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,119,354 Number of extensions: 51298 Number of successful extensions: 120 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 3 Length of query: 322 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 237 Effective length of database: 1,217,395 Effective search space: 288522615 Effective search space used: 288522615 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)