BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4450.p (565 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) syntheta... 1007 0.0 gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthet... 135 1e-32 >gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) synthetase [Herbaspirillum seropedicae SmR1] Length = 565 Score = 1007 bits (2603), Expect = 0.0, Method: Compositional matrix adjust. Identities = 496/565 (87%), Positives = 524/565 (92%) Query: 1 MLRIAIAQCNPTIGDFDGNLALHLAALQRAARAGADLLVFPELSICGYYPGDLLEEPRFL 60 MLRIAIAQCNPTIGDF+GNLALHL LQRAA+AGADL+VFPELSICGYYPGDLLEE RFL Sbjct: 1 MLRIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFL 60 Query: 61 GQAEQALQRLITATRDYPGLTAVIGAPRRNAGPGKPLFNALLALREGQIVAEYHKQLLPV 120 +AE LQ +I ATR PGLTAVIGAPRRN GPGKPLFNALLA+REG+IVAEYHKQLLPV Sbjct: 61 DRAEATLQDVIDATRRLPGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPV 120 Query: 121 YGVFDDGRHFEAGPEVACVLPLKGQRVGFLICEDGWNDELRSYRINPFQRLAEADAQLVV 180 YGVFDDGRHFEAGPE ACVLPLKG RVGFLICEDGWND+LRSYR+NPF++LA+ADAQLVV Sbjct: 121 YGVFDDGRHFEAGPETACVLPLKGMRVGFLICEDGWNDDLRSYRVNPFEKLAQADAQLVV 180 Query: 181 SINASPSDIGKRSQRHALFQAACLRRQMALLYVNQVGGQDQLVYDGGSFAMSPTQGLAFE 240 SINASPSD+GKRSQRHALFQAACLRRQMALLYVNQVGGQDQLVYDGGSFAMSP QGLAFE Sbjct: 181 SINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQDQLVYDGGSFAMSPLQGLAFE 240 Query: 241 AARLAEDFQVLAFEAGRFLRSDGSSDALPLQQALATPEFQRRQIILGLRDYARRCGFGKV 300 AARLAEDFQVLAFE GRFLRSDG + Q +ATPEF RRQI+LGLRDYARRCGFGKV Sbjct: 241 AARLAEDFQVLAFEGGRFLRSDGVGAPQCIDQTMATPEFHRRQILLGLRDYARRCGFGKV 300 Query: 301 VVGSSGGIDSALTIALAAEALGARNVVAVTMPSIFSSTGSVDDSLELCRALGVTLFRHPI 360 VVGSSGGIDSA+TIALAAEALGA+NVVAVTMPS+FSS GSV DS LC+ALGVTLFRHPI Sbjct: 301 VVGSSGGIDSAVTIALAAEALGAQNVVAVTMPSVFSSAGSVSDSETLCQALGVTLFRHPI 360 Query: 361 AELVREFEEGFARAFGAPLRGLPLENLQARVRGAILMEYSNGYGALLLTTGNKSEISVGY 420 A+LVREFE+GFA AFGAPLRGLPLENLQARVRGAILMEYSNGY ALLLTTGNKSEISVGY Sbjct: 361 ADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYDALLLTTGNKSEISVGY 420 Query: 421 CTLYGDTNGGLGLIGDLYKTEVYALARHLNASADREIIPRAIIDKPPSAELAPDQRDTDS 480 CTLYGDTNGGLGLIGDLYKTEVYALARHLNA A REIIP AIIDK PSAELAPDQRDTDS Sbjct: 421 CTLYGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAIIDKAPSAELAPDQRDTDS 480 Query: 481 LPPYEVLDDILKWHIEGTRLPADEAQAAARAVEQLQASQQGAALVERICRMVALNEYKRR 540 LPPYEVLD+ILKWHIEG+RLPADEAQAAAR VEQL + GAALVERICRMVA NEYKRR Sbjct: 481 LPPYEVLDEILKWHIEGSRLPADEAQAAARTVEQLSGTDTGAALVERICRMVARNEYKRR 540 Query: 541 QSPPVIRVRARAFGSGRQIPIAVAF 565 QSPPVIR+R+RAFGSGRQIPIAV + Sbjct: 541 QSPPVIRLRSRAFGSGRQIPIAVRY 565 >gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthetase [Herbaspirillum seropedicae SmR1] Length = 685 Score = 135 bits (339), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 156/596 (26%), Positives = 250/596 (41%), Gaps = 100/596 (16%) Query: 3 RIAIAQCNPTIGDFDGNLALHLAALQRAARAGADLLVFPELSICGYYPGDLLEEPRFLGQ 62 R+A+A + + D N +A ++AA GA L+ FPEL + Y DL ++ L Sbjct: 15 RVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAYSCEDLFQQRALLDA 74 Query: 63 AEQALQRLITATRDYPGLTAVIGAPRRNAGPGKPLFNALLALREGQIVAEYHKQLLPVYG 122 + QALQ ++ A+ P ++G P + L+N + L G+I+ K LP Y Sbjct: 75 SLQALQSVLEASMRIPA-ALIVGLPLK---VDHQLYNCAVVLHHGRILGVVPKSYLPNYS 130 Query: 123 VFDDGRHF-EAGPEVACVLPLKGQRVGF------------------LICEDGWNDELRSY 163 F + R F A V + L GQ VGF ICED W Sbjct: 131 EFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICEDVW------V 184 Query: 164 RINPFQRLAEADAQLVVSINASPSDIGKRSQRHALFQAACLRRQMALLYVNQVGGQD--Q 221 I P A A A ++V+++AS +GK RH L R A LY + G+ Sbjct: 185 PIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGESTTD 244 Query: 222 LVYDGGSFAMSPTQGLAFEAARLAEDFQVLA----------------------------- 252 L +DG + + LA E+ R A+ V+ Sbjct: 245 LAWDGQALICENGELLA-ESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHADEV 303 Query: 253 --FEAGRFLRSDGSSDALPLQQALA----TP------EFQRRQI----ILGLRDYARRCG 296 FE F ALPLQ+ +A P E + R++ + L G Sbjct: 304 RRFEVVGFEVQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLSSAG 363 Query: 297 FGKVVVGSSGGIDSALTIALAAEALGA-----RNVVAVTMPSIFSSTGSVDDSLELCRAL 351 KVV+G SGG+DS + + A+A+ N++A TMP +S+ ++ + +L + Sbjct: 364 LKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLMAQV 423 Query: 352 GVTL----FRHPIAELVREFEEGFARAFGAPLRGLPLENLQARVRGAILMEYSNGYGALL 407 G + R +++++ ++R G P+ + EN+QA R L +N +GA++ Sbjct: 424 GCSAQEIDIRPSCEQMLKDLGHPYSR--GEPVYDITFENVQAGERTNHLFRLANHHGAIV 481 Query: 408 LTTGNKSEISVGYCTL-YGDTNGGLGLIGDLYKTEVYALARHLNASADREII-PRAIID- 464 + TG+ SE+++G+CT GD + + KT + + R + S E+ P +I Sbjct: 482 IGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHMVRWVAESGSLELKQPEVLIHV 541 Query: 465 ---------KPPSAELAPDQRDTDSLPPYEVLDDILKWHIEGTRLPADEAQAAARA 511 P +++ P QR D + PYE+ D L + + PA A A A Sbjct: 542 LETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSA 597 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,724,859 Number of extensions: 73798 Number of successful extensions: 237 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 3 Length of query: 565 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 475 Effective length of database: 1,193,720 Effective search space: 567017000 Effective search space used: 567017000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)