BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4446.p (362 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313850|ref|YP_003777942.1| xenobiotic reductase A [Herbasp... 687 0.0 gi|300312734|ref|YP_003776826.1| NADH:flavin oxidoreductase [Her... 272 3e-74 gi|300312411|ref|YP_003776503.1| NADH:flavin oxidoreductase/NADH... 113 2e-26 >gi|300313850|ref|YP_003777942.1| xenobiotic reductase A [Herbaspirillum seropedicae SmR1] Length = 362 Score = 687 bits (1772), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/362 (90%), Positives = 350/362 (96%) Query: 1 MSALFTPFKLNELNLRNRIAVPPMCQYMAVDGKVNAWHLSHYASLARGGAGLVIVEATAV 60 MSALF PFKL +++LRNRIAVPPMCQYMAV+GKVN WHLSHYAS+ARGGAGLVIVEATAV Sbjct: 1 MSALFQPFKLKDVSLRNRIAVPPMCQYMAVEGKVNDWHLSHYASIARGGAGLVIVEATAV 60 Query: 61 APEGRITPACTGIWSNELAQAFVPVVQAIKAAGAVPGIQIGHAGRKASANRPWEGDDHIA 120 +PEGRITP CTGIWS+ L QAFVPVVQAIKAAGAVPGIQIGHAGRKASANRPWEGDDHIA Sbjct: 61 SPEGRITPGCTGIWSDALGQAFVPVVQAIKAAGAVPGIQIGHAGRKASANRPWEGDDHIA 120 Query: 121 ADDPRGWDTLSPSAIAYGRGLPKVPKAMSLEDIARVRQNFVDAAIRARDAGFEWLELHFA 180 ADD RGWDTLSPSAIAYGRGLPKVPKAMSL+DIARVRQ+FV+AA+RAR+AGFEWLELHFA Sbjct: 121 ADDARGWDTLSPSAIAYGRGLPKVPKAMSLDDIARVRQDFVNAAVRAREAGFEWLELHFA 180 Query: 181 HGYLGQSFFSAHTNQRDDLYGGSVENRARFLLETLRAVRQVWPQHLALTVRFGVLEYDGR 240 HGYLGQSFFSAH NQRDDLYGGSVENRARFLLETLRAVR+VWP+HLALTVRFGVLEYDGR Sbjct: 181 HGYLGQSFFSAHANQRDDLYGGSVENRARFLLETLRAVREVWPEHLALTVRFGVLEYDGR 240 Query: 241 DEQTLLESIGLVRQFKDSGMDMISVTVGFTIAEVQIPWGPAFLGPIAKRVRDEAGVPVSS 300 DE+TL+ESIG+VRQFKDSGMDMISVT+GFTI EVQIPWGPAFLGPIAKRVR+EA VPVSS Sbjct: 241 DEETLMESIGVVRQFKDSGMDMISVTIGFTIPEVQIPWGPAFLGPIAKRVREEAAVPVSS 300 Query: 301 AWGFGTPELAERAVKEGQLDLVMVGRSHLVNPHWPYQAAKELGVERPSWTLPAPYAHWLE 360 AWGFGTP LAE+AVK+GQLDLVMVGRSHLVNPHW YQAAKELGVERPSWTLPAPYAHWLE Sbjct: 301 AWGFGTPALAEQAVKDGQLDLVMVGRSHLVNPHWTYQAAKELGVERPSWTLPAPYAHWLE 360 Query: 361 RY 362 RY Sbjct: 361 RY 362 >gi|300312734|ref|YP_003776826.1| NADH:flavin oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 372 Score = 272 bits (695), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 162/343 (47%), Positives = 209/343 (60%), Gaps = 6/343 (1%) Query: 2 SALFTPFKLNELNLRNRIAVPPMCQYMAVDGKVNAWHLSHYASLARGGAGLVIVEATAVA 61 S LF+P L + L NRIAV PMCQY A DG VN WHL H S A GGAGLV++EATAV Sbjct: 3 SHLFSPLPLRGVTLPNRIAVSPMCQYSAEDGFVNDWHLVHLGSRAVGGAGLVLMEATAVV 62 Query: 62 PEGRITPACTGIWSNELAQAFVPVVQAIKAAGAVPGIQIGHAGRKASANRPWEGDDHIAA 121 EGRI+ A GIW +E + + I+ GAVPGIQ+ HAGRK+S RPW+G Sbjct: 63 AEGRISSADLGIWKDEHIAPLRRITRFIEQQGAVPGIQLAHAGRKSSILRPWDGPAGSVP 122 Query: 122 DDPRGWDTLSPSAIAYGRGLPKVPKAMSLEDIARVRQNFVDAAIRARDAGFEWLELHFAH 181 GW +PSA+ + G P+AMS+E I + Q F DAA RA AGF+ +ELH AH Sbjct: 123 PSAGGWTPQAPSALPFDDGY-STPQAMSVEQIGALVQAFADAAQRALAAGFKVIELHGAH 181 Query: 182 GYLGQSFFSAHTNQRDDLYGGSVENRARFLLETLRAVRQVWPQHLALTVRFGVLEY-DGR 240 GYLG F S +NQR D YGGS ENR RFLLE RA R VWP+ L L VR ++ DG Sbjct: 182 GYLGHQFLSPLSNQRSDEYGGSFENRIRFLLEITRATRAVWPEELPLLVRLSATDWVDG- 240 Query: 241 DEQTLLESIGLVRQFKDSGMDMISVTVGFTIAEVQIPWGPAFLGPIAKRVRDEAGVPVSS 300 T E++ L ++ ++ G+D++ V+ G + IP GP + A RV+ EAG+ S Sbjct: 241 -GWTPEETVTLSQRLRELGVDLVDVSSGGNAPKAVIPVGPGYQTSFAARVKAEAGI-ASG 298 Query: 301 AWGFGTPEL-AERAVKEGQLDLVMVGRSHLVNPHWPYQAAKEL 342 A G T + AE ++ GQ DLV++ R L +P+WP AA++L Sbjct: 299 AVGMITDAIQAEHILRTGQADLVLLARELLRDPYWPLHAAQQL 341 >gi|300312411|ref|YP_003776503.1| NADH:flavin oxidoreductase/NADH oxidase family protein [Herbaspirillum seropedicae SmR1] Length = 366 Score = 113 bits (283), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 77/225 (34%), Positives = 116/225 (51%), Gaps = 10/225 (4%) Query: 2 SALFTPFKLNELNLRNRIAVPPMCQYMAVDGKVNAWHLSHYASLARGGAGLVIVEATAVA 61 S LF P +L + L+NRI + P+ + A G V + + Y + R GAGL+I EAT ++ Sbjct: 8 SDLFKPVQLGAVQLQNRIVMAPLTRSRAQAGDVPSDLAAEYYA-QRAGAGLIIAEATQIS 66 Query: 62 PEGRITPACTGIWSNELAQAFVPVVQAIKAAGAVPGIQIGHAGRKASANRPWEGDDHIAA 121 PEG+ GI+++ A+ + A+ A G +Q+ H GR + P H Sbjct: 67 PEGKGYAWTPGIYNDAHVAAWKKITDAVHAKGGRIFLQLWHVGR---ISHPDLQPGHAL- 122 Query: 122 DDPRGWDTLSPSAIAYGRGLPK---VPKAMSLEDIARVRQNFVDAAIRARDAGFEWLELH 178 P + P A+ K P+A+ ++ + + + AA + AGF+ +E+H Sbjct: 123 --PVAPSAVQPEGQAFTESGFKPFVTPRALEAAELPAIVEQYKKAAQLSMQAGFDGVEIH 180 Query: 179 FAHGYLGQSFFSAHTNQRDDLYGGSVENRARFLLETLRAVRQVWP 223 A+GYL F TNQR D YGGS+ENRAR LLE ++AV V P Sbjct: 181 AANGYLLDQFLRDKTNQRTDGYGGSIENRARLLLEVVQAVTSVVP 225 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,254,356 Number of extensions: 56023 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 3 Length of query: 362 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 276 Effective length of database: 1,212,660 Effective search space: 334694160 Effective search space used: 334694160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)