BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4434.p (506 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 884 0.0 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 195 7e-51 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 185 8e-48 gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 123 4e-29 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 100 3e-22 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 884 bits (2283), Expect = 0.0, Method: Compositional matrix adjust. Identities = 455/478 (95%), Positives = 466/478 (97%) Query: 29 LPDSLLGRLSVVMIFGVLVTQVAGGMIWAAQLRSKSEAETRIAAQHLAHSAASAIRYFVS 88 +PDSLLGRLSVVMIFGVLVTQVAGG+IWA QLRSKSEAETR AAQHLAHSAASAIRYF++ Sbjct: 1 MPDSLLGRLSVVMIFGVLVTQVAGGLIWAVQLRSKSEAETRTAAQHLAHSAASAIRYFMT 60 Query: 89 LPPNYRPLMIQQLREMGGTRFFINANSSAVPVQSIGPRHLADLALEVTAQTLREDLPFLP 148 LPPNYRPLMIQQLREMGGTRFFINANSSAV V+SIG LA LA++V AQTLREDLPFLP Sbjct: 61 LPPNYRPLMIQQLREMGGTRFFINANSSAVAVKSIGDSRLAKLAIDVAAQTLREDLPFLP 120 Query: 149 QARLAFAWPDGLPVASDGLTVNDLPDNWVQHILLTKPNSAPVLVIQMQVESGRWIYLAAL 208 +ARLAFAWPDGLPVASDGLTVNDLPDNWVQHIL+TKPNSAPVLVIQMQVESGRWIYLAAL Sbjct: 121 EARLAFAWPDGLPVASDGLTVNDLPDNWVQHILMTKPNSAPVLVIQMQVESGRWIYLAAL 180 Query: 209 MPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVP 268 MPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVP Sbjct: 181 MPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVP 240 Query: 269 RLPESGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDA 328 LPE+GSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDA Sbjct: 241 SLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDA 300 Query: 329 MRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLGGHEIAFTPS 388 MRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARL GHEIAFTPS Sbjct: 301 MRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLAGHEIAFTPS 360 Query: 389 GINVMAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAM 448 GINV+AKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAM Sbjct: 361 GINVIAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAM 420 Query: 449 RTLFQPYVRLEHGRASNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR 506 TLFQPYVRLEHGRA NSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR Sbjct: 421 GTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR 478 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 195 bits (496), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 141/488 (28%), Positives = 234/488 (47%), Gaps = 37/488 (7%) Query: 30 PDSLLGRLSVVMIFGVLVTQVAGGMIWA-AQLRSKSEAETRIAAQHLAHSAASAIRYFVS 88 P +L GRL+++++ G+L Q+ G IW + + E TR+AA HLA A+ Sbjct: 15 PQTLSGRLALILVAGMLAAQMLTGTIWYDMRYGTAMEMPTRLAAAHLA----DAVLILDD 70 Query: 89 LPPNYRPLMIQQLREMGGTRFFIN----ANSSAVPVQSIGP-----RHLADLALEVTAQT 139 P R + L +F ++ + + + V + P H +++ Sbjct: 71 APATQRQALAHSLTR---AQFVLSLPDVVDRADLAVPGLAPASSPTTHADQGVVDLFGTV 127 Query: 140 LREDLPFLPQARLAFAWPDGLPVASDG---LTVNDLPDNWVQHILLTKPNSAPVLVIQMQ 196 L++ P L D A L ++ P + ++ K S +Q Sbjct: 128 LQQHAGPRPWKMLQLGLDDQHANAESSWWTLLRDEFPHGRFRVLVTLKDGS----WLQAD 183 Query: 197 VESGRWIYLAALMPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALAD 256 +E G+ A + P+ D + + L + A++L+ + VR +PL +A+ Sbjct: 184 IEEGQ----AGMSTAPHSAAFDY-----FMRIYALRIFAVVLIAWMAVRLAMKPLQVMAE 234 Query: 257 AAEAFGKGETVPRLPESGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITR 316 AAE G L G E A+AF+ M+ RI + +R R ++SHDLR+PITR Sbjct: 235 AAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQRRIIDNIAERTRFLAAVSHDLRSPITR 294 Query: 317 LKLRTELMDDDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDA 376 +KLRTE++ D+ + F DLDE+D ++ L V+D D+ E + LD+L+ + D Sbjct: 295 MKLRTEMLPDERGKEKFRKDLDEMDALISATLNVVQDIDVQERPQLIDLDSLLESLRTDQ 354 Query: 377 RLGGHEIAFTPSGINVMAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEI 436 G E+ + + P +L+R + NL +NA+ YGQ+ + ++ + D V I + Sbjct: 355 AEVGGEVTLSGHAAPLQGYPRSLRRCLQNLVENAVRYGQRARVQLQD----DADQVCIRV 410 Query: 437 RDHGPGVPEDAMRTLFQPYVRLEHGRASNSGGMGLGLGISRNIVQAHGGELLLRNHPEGG 496 D GPG+PE + + +P+ RLE R + +GG GLGL I+ +VQAH G + L N P GG Sbjct: 411 DDDGPGIPEALLERVQEPFYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGG 470 Query: 497 LMATIILP 504 L I++P Sbjct: 471 LRVEILIP 478 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 185 bits (469), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 107/275 (38%), Positives = 154/275 (56%), Gaps = 5/275 (1%) Query: 231 LSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVPRLPESGSREYVKTARAFSGMRE 290 L L I +L LV R TRP+ LA AA G L E+G E + A AF+ M+ Sbjct: 187 LFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAHAFNAMQA 246 Query: 291 RIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDAMRTDFHDDLDELDMMVKGALQS 350 RI+R ++ R + +I+HDL+TP+TR++LR E + D ++ DDL + MV+ L Sbjct: 247 RIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVSDPGLQQKLIDDLGVMHGMVREGLDL 306 Query: 351 VKDSDIHENRTEVRLDALVLRMIRDARLGGHEIAFTPSG-INVMAKPLALKRAIGNLFDN 409 + D E + +D+L+ + DA G ++A S VMA+P AL+R + NL DN Sbjct: 307 ARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVMAQPGALRRCLTNLIDN 366 Query: 410 ALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAMRTLFQPYVRLEHGRASNSGGM 469 A YG+ +SI E + I IRD GPG+PE + +F+P+ RLE R+ ++GG Sbjct: 367 ACKYGKSARVSI----ALEQQKIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTGGT 422 Query: 470 GLGLGISRNIVQAHGGELLLRNHPEGGLMATIILP 504 GLGL I+ NI + H G+L LRN EGGL + LP Sbjct: 423 GLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELP 457 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 123 bits (308), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 101/284 (35%), Positives = 134/284 (47%), Gaps = 18/284 (6%) Query: 231 LSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVPRLPESGSREYVKTARAFSGMRE 290 LSL + ++ L+ +PLA L AA A KG LPESG E + R+F+ M Sbjct: 172 LSLIGAVFISTLI----NQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVA 227 Query: 291 RIKRYLEDRERLFVSISHDLRTPITRLKLRTELMD-DDAMRTDFHDDLDELDMMVKGALQ 349 + R DR + ISHDLRTP+ R++L E+ + D R H DL ++D ++ L Sbjct: 228 DLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLD 287 Query: 350 SVKDSDIHENRTEVRLDALVLRMIRDARLGGHEI--AFTPSGINVMAKPLALKRAIGNLF 407 K D T D L +RL + TP V L R NL Sbjct: 288 YAKPFDTSSLETIDLADVLQDVAGNASRLSDIRVHTQITPD-TQVAGNGTELARVFSNLV 346 Query: 408 DNALYYGQK-----VEISIRPSPGAEGDLVEIEIRDHGPGVPEDAMRTLFQPYVRLEHGR 462 +NA YG+ EI IR EG V +E+ DHGPGVP + L +P+ RL+ R Sbjct: 347 ENARRYGKTEGTDCAEIDIRSR--IEGHQVVVEVADHGPGVPVNECERLLRPFTRLDTAR 404 Query: 463 ASNSGGMGLGLGISRNIVQAHGGELLLRNHPE--GGLMATIILP 504 + G GLGL I IV H G+LLL+ H E GL+ I LP Sbjct: 405 G-QANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLP 447 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 100 bits (249), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 83/296 (28%), Positives = 139/296 (46%), Gaps = 25/296 (8%) Query: 231 LSLAAILLLTILVVRWT---TRPLAALADAAEAFGKGETVPRLPESGSREYVKTARAFSG 287 +++ A++L LVVR + TRPL +A A G+ R+ E A+ F+ Sbjct: 177 IAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDEVGMLAQDFNQ 236 Query: 288 MRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDAMRTD------FHDDLDELD 341 + ++R + R +SH+LRTP+ L + E ++D ++ D +++ L+ Sbjct: 237 LAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSEVEHLN 296 Query: 342 MMVKGALQSVKDSD---IHENRTEVRL-----DALVLRMIRDARLGGHEIAFTPSGINVM 393 +V G L + +D + R ++ L D+ L R ++ G P+ + V Sbjct: 297 KLV-GDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMPVH 355 Query: 394 AKPLALKRAIGNLFDNALYY---GQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAMRT 450 L++ + NLF+N+L Y G ++++ + + D PGVP + M Sbjct: 356 GDAARLQQLLSNLFENSLRYTDAGGRLQVHAH----LQQQHWHLVFDDSEPGVPPEVMAR 411 Query: 451 LFQPYVRLEHGRASNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR 506 LF+ + R+E R SGG GLGL I R IV AH G + P GGL ++LPAR Sbjct: 412 LFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPAR 467 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,463,489 Number of extensions: 59425 Number of successful extensions: 319 Number of sequences better than 1.0e-08: 36 Number of HSP's gapped: 289 Number of HSP's successfully gapped: 36 Length of query: 506 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 417 Effective length of database: 1,198,455 Effective search space: 499755735 Effective search space used: 499755735 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)