BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4432.p (154 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313827|ref|YP_003777919.1| hypothetical protein Hsero_4545... 238 2e-64 >gi|300313827|ref|YP_003777919.1| hypothetical protein Hsero_4545 [Herbaspirillum seropedicae SmR1] Length = 146 Score = 238 bits (606), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 120/144 (83%), Positives = 130/144 (90%), Gaps = 2/144 (1%) Query: 5 SPDPHSPLPGSRLAPLRTRWLWLLLAYVSLGIGIVALFIPGIPTTEFVLLSAWAASRGSP 64 +P+ + S LAP RTRWLWLLLAY SLG+GIVA+FIPGIPTTEFVLLSAWAA +GSP Sbjct: 3 APEQRASSAPSTLAPARTRWLWLLLAYASLGVGIVAIFIPGIPTTEFVLLSAWAAGKGSP 62 Query: 65 RLQRWLEQHRLFGPMIHNWRHGQVVARRAKISASLMMSLCLVIMLFTVKRHWVIVLATLG 124 RLQRWLEQHRLFGPMIHNWRHG+VVARRAKISASLMMSLCLVIMLFTVKRHWVIVLATLG Sbjct: 63 RLQRWLEQHRLFGPMIHNWRHGRVVARRAKISASLMMSLCLVIMLFTVKRHWVIVLATLG 122 Query: 125 MAVGAAWMWSRPERIEADNALPGS 148 MA+GAAWMWSRPER A++A PG Sbjct: 123 MALGAAWMWSRPER--AEDAEPGE 144 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 490,132 Number of extensions: 18085 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 1 Length of query: 154 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 77 Effective length of database: 1,255,275 Effective search space: 96656175 Effective search space used: 96656175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)