BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4431.p (196 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313826|ref|YP_003777918.1| heme oxygenase [Herbaspirillum ... 278 2e-76 >gi|300313826|ref|YP_003777918.1| heme oxygenase [Herbaspirillum seropedicae SmR1] Length = 196 Score = 278 bits (711), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 154/196 (78%), Positives = 168/196 (85%) Query: 1 MNIVSPQAAPLSARLKTETAALHEQMHALMESAQPFSSRAHYARFVAAQYHFQRDVQHLF 60 M V+ A LSARLK +TAALHEQMHALME AQPF+ RA YA+FVAAQYHFQRDV+HLF Sbjct: 1 MKPVATPAPSLSARLKADTAALHEQMHALMEQAQPFADRARYAQFVAAQYHFQRDVEHLF 60 Query: 61 DDPALQAAVPDLQVRGREAAALADLADLDAEPGALAIATATVQLPEALGWLYVSEGSTLG 120 DDPALQAAVPDLQVRGREA ALADLADL A PG + IATATV++PEALGWLYVSEGSTLG Sbjct: 61 DDPALQAAVPDLQVRGREADALADLADLGAVPGQIDIATATVRMPEALGWLYVSEGSTLG 120 Query: 121 AAFLLKEVKAKLGLREDFGARNLAAYPEGRALVWRRFVGFLDAPGLSAQQQSAVIAGAAA 180 AAFLLK+V+ +LGL E FGARNLAAY EGRA+VWRRFVGFLD G S Q Q AV+AGA A Sbjct: 121 AAFLLKQVQQRLGLDEQFGARNLAAYAEGRAVVWRRFVGFLDQAGDSPQVQEAVLAGARA 180 Query: 181 AFARFGWLLRQHFALA 196 AF RFG LLR+HFALA Sbjct: 181 AFERFGALLRRHFALA 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.132 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 554,088 Number of extensions: 19189 Number of successful extensions: 108 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 1 Length of query: 196 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 116 Effective length of database: 1,241,070 Effective search space: 143964120 Effective search space used: 143964120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)