BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4428.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport tran... 786 0.0 gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport tran... 393 e-110 gi|300313118|ref|YP_003777210.1| proton glutamate symport protei... 221 9e-59 >gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 786 bits (2030), Expect = 0.0, Method: Compositional matrix adjust. Identities = 422/442 (95%), Positives = 434/442 (98%) Query: 1 VSKVFKSLFGQVVLALMAGIVIGLFWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGI 60 VSK+FKSLFGQVVLAL+ GI+IGLFWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGI Sbjct: 2 VSKLFKSLFGQVVLALIGGIIIGLFWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGI 61 Query: 61 CGASDLKKVGSVGVKAIIYFEVVTTVALMLGLVLALVVQPGAGMNIDPSNLDASSLSSYM 120 CGASDLKKVGSVGVKAIIYFEVVTT+AL+LGLVLALVVQPGAGMNIDPSNLDASSLS YM Sbjct: 62 CGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYM 121 Query: 121 ANAGKVKEAGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALV 180 ANAGKVKE GFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALV Sbjct: 122 ANAGKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALV 181 Query: 181 SSLSDTFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVL 240 +SLSD FFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVL Sbjct: 182 ASLSDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVL 241 Query: 241 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMRKLEHMGIKKSTVGLVIPTGY 300 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIM+KLEHMGIKKSTVGLVIPTGY Sbjct: 242 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPTGY 301 Query: 301 SFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATL 360 SFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATL Sbjct: 302 SFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATL 361 Query: 361 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWEKDIDKVRARAVLNGEN 420 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWE+DIDKVRARAVLNGE+ Sbjct: 362 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWERDIDKVRARAVLNGES 421 Query: 421 VAPLVDETPAIDLLGVTGKAAQ 442 VAPLV+E P I+L+GVT K AQ Sbjct: 422 VAPLVEENPVIELMGVTVKPAQ 443 >gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 393 bits (1009), Expect = e-110, Method: Compositional matrix adjust. Identities = 216/433 (49%), Positives = 304/433 (70%), Gaps = 9/433 (2%) Query: 4 VFKSLFGQVVLALMAGIVIGLFWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGICGA 63 ++KSL+ QV++A++ GI++G F+P G+ +KPLGDGF+KLIKMII ++FC VV GI G Sbjct: 8 LYKSLYFQVLVAIVIGILLGHFYPSTGEAMKPLGDGFVKLIKMIIAPVIFCTVVIGIAGM 67 Query: 64 SDLKKVGSVGVKAIIYFEVVTTVALMLGLVLALVVQPGAGMNIDPSNLDASSLSSYMANA 123 D+KKVG G A++YFEVV+TVAL++GL+L +QPG GMN+DP++LD S+++Y A Sbjct: 68 EDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDPASLDTKSIAAYTAPG 127 Query: 124 GKVKEAGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALVSSL 183 K +F++ +IP + V AF GDVLQVLL++V FG AL G +G V + + Sbjct: 128 ---KMGSVTDFVLGIIPTSMVDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDFIEKI 184 Query: 184 SDTFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVLGGI 243 S F + +++AP+G GA++FT+GKYG+GSL LA L+ FY + F+ VVLG I Sbjct: 185 SHVLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVVLGII 244 Query: 244 LRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMRKLEHMGIKKSTVGLVIPTGYSFN 303 R G SI+K ++Y++EEL++VL T+SS+SVLP+++ K+E+ G KK+ VGLVIPTGYSFN Sbjct: 245 TRLHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTGYSFN 304 Query: 304 LDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATLTTI 363 LD +IYLTMAA+FIAQATNT ++ +L + L+TSKGA G+ GS ++LAATL+ + Sbjct: 305 LDGTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAATLSAV 364 Query: 364 PAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWEKDIDKVRARAVLNGENV-- 421 +PV GL L+L ID F+ ARAL N +GN VAT+V+A W ++D R VLN E V Sbjct: 365 GHVPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNETVED 424 Query: 422 --AP--LVDETPA 430 AP ++D T A Sbjct: 425 AQAPESILDRTDA 437 >gi|300313118|ref|YP_003777210.1| proton glutamate symport protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 221 bits (563), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 135/372 (36%), Positives = 216/372 (58%), Gaps = 19/372 (5%) Query: 38 DGFIKLIKMIIPVIVFCVVVQGICGASDLKKVGSVGVKAIIYFEVVTTVALMLGLVLALV 97 D F++LIKM+I +VF +V GI D K VG + K++ +F + + V+L LG+++A + Sbjct: 46 DLFLRLIKMVIAPLVFSTLVVGIAHMGDAKSVGRIFGKSLAWFFIASLVSLALGMIMANL 105 Query: 98 VQPGAGMNIDPSNLDASSLSSYMANAGKVKEAGFAEFIMKLIPATAVSAFTSGDVLQVLL 157 +QPGAG+ + + + L++ + VKE F L+P + V A +VLQV++ Sbjct: 106 LQPGAGVALPSPDAAGAGLAT---SKFTVKE-----FFNHLVPKSIVEAMAQNEVLQVVV 157 Query: 158 ISVTFGCALLLIGEKGAPVVALVSSLSDTFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGS 217 S+ FG AL +GE+G ++A++ LS T K + ++ AP+ V A+A TV G+ Sbjct: 158 FSMFFGIALASLGERGKHLLAVIDDLSHTMLKITVYVMKFAPVAVFAAMAATVAVNGLEI 217 Query: 218 LKQLALLVLLFYGAVIFFVLVVLGGILRASGL-----SIFKLIRYLREELVVVLATTSSD 272 L A+ + FY F LV+L IL A G IF L+ ++E ++ AT SS+ Sbjct: 218 LVSFAVFMRDFY-----FSLVLLWVILIAVGFIFLKKRIFHLLALIKEAFLLAFATASSE 272 Query: 273 SVLPQIMRKLEHMGIKKSTVGLVIPTGYSFNLDAFSIYLTMAALFIAQATNTHLSMGDLA 332 + P+++ L+ G+K+ V+P GYSFNLD IY T A LFIAQA H+ + Sbjct: 273 AAYPKLLDALDRFGVKRKISSFVMPMGYSFNLDGSMIYCTFATLFIAQAYGIHMPISTQI 332 Query: 333 AILAIALVTSKGAHGVPGSAIVILAATLTTIPAIPVVGLVLVLSIDWFIGIARALGNLLG 392 ++ + ++TSKG GVP +++V++AATL IP GL+++L ID F+ + R+ N +G Sbjct: 333 TMMLVLMLTSKGIAGVPRASLVVIAATLHQFN-IPEAGLLVILGIDTFLDMGRSATNAVG 391 Query: 393 NCVATVVIASWE 404 N +A+ V+A WE Sbjct: 392 NSIASAVVAKWE 403 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,283,012 Number of extensions: 52512 Number of successful extensions: 270 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 265 Number of HSP's successfully gapped: 3 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)