BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4427.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidoh... 686 0.0 gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase... 410 e-116 gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum ... 168 7e-43 >gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 686 bits (1769), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/417 (83%), Positives = 381/417 (91%) Query: 4 IHAMAQPFRQAGQQAMQWAEQLARHTETPGMLTRTYLTPMHQAAASELAHWMEQAGMSVR 63 + A+ +PF+QAG+QAMQWA+QLA+HTE+PGMLTRTYLTP HQAAA+ LA WME+AGM VR Sbjct: 1 MKAVPEPFQQAGRQAMQWAQQLAQHTESPGMLTRTYLTPQHQAAAAALARWMEEAGMRVR 60 Query: 64 RDNAGNVIGRYEAAAGHEQAPALVTGSHFDTVRNAGKYDGNLGIVLPLACIAQWHRAGRR 123 RDNAGNVIGRYEAA GHE AP+ VTGSHFDTVRN G YDGNLGIVLPLACIA+WHR G+R Sbjct: 61 RDNAGNVIGRYEAAPGHESAPSFVTGSHFDTVRNGGWYDGNLGIVLPLACIARWHRQGQR 120 Query: 124 FAFPIEVIGFAEEEGVRFKATLLGSRTVAGSFDQAALDHLDSAGISMREAMQAVGFDPAR 183 FAFPIEVIGFAEEEGVRFKATLLGSRTVAG+FDQA L++ DS G++MR A++A G DP Sbjct: 121 FAFPIEVIGFAEEEGVRFKATLLGSRTVAGTFDQAVLENRDSDGVTMRAAIRAAGLDPDG 180 Query: 184 IAADAWPRGSVAAFVEVHIEQGPTLLNENLPLGVVTAISGATRFMGEVRGLAGHAGTVPM 243 IAADAW GS+AAFVEVHIEQGP LLNE+LP+GVVTAISGA+RFM EV GLAGHAGTVPM Sbjct: 181 IAADAWQPGSMAAFVEVHIEQGPLLLNEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPM 240 Query: 244 HLRRDAAMAAAEIGLYIERRCGIKPGLVGTMGILEVVQGAANVVPGLASFSIDIRAERDE 303 HLRRDAAMAAAEIGLYIERRC IKP LVGTMG+LEVVQGAANVVPGLASFSIDIRAE D Sbjct: 241 HLRRDAAMAAAEIGLYIERRCSIKPELVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDS 300 Query: 304 DRLAAVADVTAEIERIAARRQVEVALRRTHEAASVPCAPRLQAALAQAIEAAGWPVRHLP 363 DRLAAVADV AEI+RIAARRQV++ALR+THEAASVPCAPRLQAALAQ+I AAGWPVRHLP Sbjct: 301 DRLAAVADVKAEIDRIAARRQVQLALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLP 360 Query: 364 SGAGHDAMALAPLCEVAMLFVRCGNGGISHHPDETMTTDDACIAAQVFSHLVEQFQP 420 SGAGHDAMALA L +VAMLFVRCGNGGISHHPDETMT DA IAAQVFSHLVEQFQP Sbjct: 361 SGAGHDAMALAGLADVAMLFVRCGNGGISHHPDETMTEADAAIAAQVFSHLVEQFQP 417 >gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase/N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 410 bits (1055), Expect = e-116, Method: Compositional matrix adjust. Identities = 210/407 (51%), Positives = 273/407 (67%), Gaps = 4/407 (0%) Query: 15 GQQAMQWAEQLARHTETPGMLTRTYLTPMHQAAASELAHWMEQAGMSVRRDNAGNVIGRY 74 G MQWAE++ +E G LT TYLT HQ A+++A WM +AGM V D GNV+GRY Sbjct: 180 GATVMQWAEEIGALSEEEGALTCTYLTETHQRTAAQIAQWMREAGMQVHIDAVGNVVGRY 239 Query: 75 EAAAGHEQAPALVTGSHFDTVRNAGKYDGNLGIVLPLACIAQWHRAGRRFAFPIEVIGFA 134 ++ H QA L+TGSH+DTVRN GKYDG GI+LP+A + H G F +EVIGF+ Sbjct: 240 QSP--HPQAKTLLTGSHYDTVRNGGKYDGREGILLPIAVVKYLHERGETLPFHLEVIGFS 297 Query: 135 EEEGVRFKATLLGSRTVAGSFDQAALDHLDSAGISMREAMQAVGFDPARIAADAWPRGSV 194 EEEGVRFK+T LGS + G FD A LD D ++MRE + G D A I A + Sbjct: 298 EEEGVRFKSTFLGSNAITGHFDMALLDLRDKEDVTMRELIIQAGHDVAAIPQIARDPAEI 357 Query: 195 AAFVEVHIEQGPTLLNENLPLGVVTAISGATRFMGEVRGLAGHAGTVPMHLRRDAAMAAA 254 A+VEVHIEQGP LL +LP+G+VT+I+G++R++ +++G+A HAGT PM +R+DAA AAA Sbjct: 358 LAYVEVHIEQGPVLLKRDLPVGIVTSIAGSSRYLVDLKGVASHAGTTPMDMRKDAAAAAA 417 Query: 255 EIGLYIERRCGIKP--GLVGTMGILEVVQGAANVVPGLASFSIDIRAERDEDRLAAVADV 312 EI LY+E+RC LVGT+G L+V +G+ NV+PG S+DIRA DE RLAAV D+ Sbjct: 418 EIILYVEQRCSQDQHAALVGTVGQLQVPRGSTNVIPGACQLSLDIRAATDEVRLAAVKDI 477 Query: 313 TAEIERIAARRQVEVALRRTHEAASVPCAPRLQAALAQAIEAAGWPVRHLPSGAGHDAMA 372 A+IE I RR +++ L T A + PCAP L A L+ A + AG P L SGAGHDAMA Sbjct: 478 LAKIEEICTRRSIDLKLEETLSAPAAPCAPWLMAQLSAATQRAGVPPFELASGAGHDAMA 537 Query: 373 LAPLCEVAMLFVRCGNGGISHHPDETMTTDDACIAAQVFSHLVEQFQ 419 + L +V MLF RCGNGGISH+P ETMT DDA ++ Q+ + F+ Sbjct: 538 MVRLTDVCMLFTRCGNGGISHNPLETMTADDAEVSGQILLDFLRNFK 584 >gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 168 bits (426), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 122/383 (31%), Positives = 184/383 (48%), Gaps = 22/383 (5%) Query: 24 QLARHTET-PGMLTRTYLTPMHQAAASELAHWMEQAGMSVRRDNAGNVIGRYEAAAGHEQ 82 +LAR T G + R LT + + ++ W GMSV D GN+ R G Sbjct: 23 ELARIGATAKGGVCRLALTDLDKQGRDLVSSWARAEGMSVVVDQIGNIFMR---RPGRNN 79 Query: 83 A-PALVTGSHFDTVRNAGKYDGNLGIVLPLACIAQWHRAGRRFAFPIEVIGFAEEEGVRF 141 A P ++TGSH DT GK+DGN G++ + + ++ + PIEV + EEG RF Sbjct: 80 ALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVVRTLNQHNIQTEAPIEVAVWTNEEGSRF 139 Query: 142 KATLLGSRTVAGSFDQAALDH----LDSAGISMREAMQAVGFDPARIAADAWPRGSVAAF 197 ++GS G+F LDH D G +R+ ++ +G+ ++ D + A+ Sbjct: 140 VPVMMGSGVFCGAF---TLDHAYNATDVEGKKVRDELERIGYIGEQVCGDH----PIGAY 192 Query: 198 VEVHIEQGPTLLNENLPLGVVTAISGATRFMGEVRGLAGHAGTVPMHLRRDAAMAAAEIG 257 E HIEQGP L N + +GVV A+ G + + V G HAG PM +R+DA + I Sbjct: 193 YEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEIRKDALQVSTYIM 252 Query: 258 ---LYIERRCGIKPGLVGTMGILEVVQGAANVVPGLASFSIDIRAERDEDRLAAVADVTA 314 + I R P GT+G ++V+ + NV+PG FSID+R + D+ A Sbjct: 253 QEVVAIAHR--YAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEASLTRMHEDLLA 310 Query: 315 EIERIAARRQVEVALRRTHEAASVPCAPRLQAALAQAIEAAGWPVRHLPSGAGHDAMALA 374 +E + + + + L R P P + A A G+ + SGAGHDA+ A Sbjct: 311 YLEAMRKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVSGAGHDAIYAA 370 Query: 375 PLCEVAMLFVRCGNGGISHHPDE 397 L M+FV C + G+SH+ E Sbjct: 371 RLAPAGMIFVPCKD-GVSHNEIE 392 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,380,493 Number of extensions: 61455 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 3 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)