BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4426.p (550 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313821|ref|YP_003777913.1| histidine ammonia-lyase [Herbas... 1041 0.0 gi|300313003|ref|YP_003777095.1| histidine ammonia-lyase [Herbas... 196 4e-51 >gi|300313821|ref|YP_003777913.1| histidine ammonia-lyase [Herbaspirillum seropedicae SmR1] Length = 542 Score = 1041 bits (2692), Expect = 0.0, Method: Compositional matrix adjust. Identities = 516/540 (95%), Positives = 528/540 (97%) Query: 11 TITIDGFTLTAAQVLSVARAPHLKVALAESSRAALKQSRDYIESTWMHDEAPMMYSFNTG 70 TITIDGF LTAAQV+SVARAPHLKVALA+SSRAALKQSRDYIESTWMHDEAPMMYSFNTG Sbjct: 3 TITIDGFNLTAAQVVSVARAPHLKVALADSSRAALKQSRDYIESTWMHDEAPMMYSFNTG 62 Query: 71 VGLLKDTRIKVEHIELFQTQMIRAHSAGIGEPFSEEVSRATMLLRANAFASNYSAPRVEV 130 VGLLKDTRIKVEHIELFQTQ+IRAHSAGIGE FSEEVSRATMLLRANAFASNYSAPRVEV Sbjct: 63 VGLLKDTRIKVEHIELFQTQLIRAHSAGIGEAFSEEVSRATMLLRANAFASNYSAPRVEV 122 Query: 131 VDRLLAFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIAGFDEAEVMYQGQRMRATQAIE 190 VDRLLAFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIAGFDEAEVMYQGQRMRAT+AIE Sbjct: 123 VDRLLAFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIAGFDEAEVMYQGQRMRATEAIE 182 Query: 191 QAGIGPVKFDLKAKDASALINGCTASLAVAILAAHDARNLLTDACLSLGLTLEAMRAEMS 250 QAGIGPVKFDLKAKDASALINGCTASLAVAILAAHDAR+LLTDACLSLGLTLEAMRAEMS Sbjct: 183 QAGIGPVKFDLKAKDASALINGCTASLAVAILAAHDARHLLTDACLSLGLTLEAMRAEMS 242 Query: 251 AFDARIQQARPHAGQIKTAAVIRTLLNGSTRTTHDARAVQLPDELRRTDIPYTARIQDVY 310 AFD RIQQARPHAGQIKTA VIRTLL GSTRTTH ARAVQLPDELRRTDI YTARIQDVY Sbjct: 243 AFDPRIQQARPHAGQIKTAEVIRTLLQGSTRTTHAARAVQLPDELRRTDIAYTARIQDVY 302 Query: 311 SLRCAPQVYGPVFDALDYIDTIIEKETNSATDNPLIFNKEDGEGFEIISGGNFHGQYLAQ 370 SLRCAPQVYGPVFDALDYIDTIIEKETNSATDNPLIF+KE+GEGFEIISGGNFHGQYLAQ Sbjct: 303 SLRCAPQVYGPVFDALDYIDTIIEKETNSATDNPLIFHKEEGEGFEIISGGNFHGQYLAQ 362 Query: 371 AMDLLAMAITDLGSICERRIARLIDPTLSWGLPRNLMSGIRGVNTGYPVVQCSMSSLVME 430 AMDLLAMAITDLGSICERRIARLIDPTLSWGLPRNLMSG+RGVNTGYPVVQCSMSSLVME Sbjct: 363 AMDLLAMAITDLGSICERRIARLIDPTLSWGLPRNLMSGVRGVNTGYPVVQCSMSSLVME 422 Query: 431 NRTLCMPGSVDSIPSKGNSEDHISNSTWCARKAMTVVQNTQYIVGVEMLLAAQALTMTDD 490 NRTLCMPGSVDSIPSKGNSEDHISNSTWCARKAMTVVQNTQYIVGVEMLLAAQALTMT+ Sbjct: 423 NRTLCMPGSVDSIPSKGNSEDHISNSTWCARKAMTVVQNTQYIVGVEMLLAAQALTMTEA 482 Query: 491 LLKDFTLGQGTQAAYAEIRCQIPACLDGDRWFHDDVQLAHDFVVSGSVRNAVIAAIGDFL 550 LLKDF LGQGTQAAYAEIR QIPACLDGDRWFHDDVQ AH FVVSGSVRNAV+AAIG+F+ Sbjct: 483 LLKDFVLGQGTQAAYAEIRRQIPACLDGDRWFHDDVQAAHGFVVSGSVRNAVLAAIGEFV 542 >gi|300313003|ref|YP_003777095.1| histidine ammonia-lyase [Herbaspirillum seropedicae SmR1] Length = 526 Score = 196 bits (499), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 149/480 (31%), Positives = 238/480 (49%), Gaps = 44/480 (9%) Query: 42 RAALKQSRDYIESTWMHDEAPMMYSFNTGVGLLKDTRIKVEHIELFQTQMIRAHSAGIGE 101 RA + + D+++ D +Y TG G + E + + H G+G Sbjct: 46 RARIARGADFLDRLLREDGT--IYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGA 103 Query: 102 PFSEEVSRATMLLRANAFASNYSAPRVEVVDRLLAFINAGIHPIMPQKGSVGASGDLAPL 161 F+ E +RA + R N+ +S V +++++ + + P +P +GSVGASGDL PL Sbjct: 104 LFTPEQARAILAARLNSLCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPL 163 Query: 162 AYLAAAIAGFDEAEVMYQGQRMRATQAIEQAGIGPVKFDLKAKDASALINGCTASLAVAI 221 +YLAA + G E +V +G + A QA+ AG+ P++ L+ K+ A++NG A+A Sbjct: 164 SYLAAVLCG--ERDVWREGATVPAAQALAAAGMTPLR--LRPKEGLAIMNGTAVMTALAC 219 Query: 222 LAAHDARNLLTDACLSLGLTLEAMRAEMSAFDARIQQARPHAGQIKTAAVI-RTLLNGST 280 LA AR L A ++ + FDA + A+PH GQ + AA + R L G Sbjct: 220 LAFDRARYLCQLATRITAMSSFTLDGNAHHFDATLFSAKPHPGQQQVAAWLQRDLPCGQA 279 Query: 281 RTTHDARAVQLPDELRRTDIPYTARIQDVYSLRCAPQVYGPVFDALDYIDTIIEKETNSA 340 R+QD YS+RCAP V G + DAL + IE E NSA Sbjct: 280 HRNEK-------------------RLQDRYSVRCAPHVIGVLNDALPSLRQFIENELNSA 320 Query: 341 TDNPLIFNKEDGEGFEIISGGNFHGQYLAQAMDLLAMAITDLGSICERRIARLIDPTLSW 400 DNPLI D +G ++ GG+F+G ++A AMD + A+ ++ + +R++A ++D + Sbjct: 321 NDNPLI----DPDGERVLHGGHFYGGHIAFAMDSMKTAVANVADLLDRQMALMVDQRYNN 376 Query: 401 GLPRNLMSGIRG----VNTGYPVVQCSMSSLVMENRTLCMPGSVDSIPSKGNSEDHISNS 456 GLP NL SG +G +N G +Q S S+ E L MP SV S ++ +++D +S Sbjct: 377 GLPANL-SGAQGARAPINHGLKALQISASAWTAEALKLTMPASVFSRSTECHNQDKVSMG 435 Query: 457 TWCARKAMTVVQNTQYIVGVEML-------LAAQALTMTDDLLKDFTLGQ--GTQAAYAE 507 T AR + V++ T+ + ++ L +Q + D L+D G+ QAA +E Sbjct: 436 TIAARDCLRVLELTEQVAAALLITVRQGVWLRSQRQQVGDTPLQDLPAGRLAAMQAALSE 495 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,542,469 Number of extensions: 60011 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 2 Length of query: 550 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 460 Effective length of database: 1,193,720 Effective search space: 549111200 Effective search space used: 549111200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)