BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4418.p (356 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 595 e-171 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 180 1e-46 gi|300313291|ref|YP_003777383.1| porin transmembrane protein [He... 151 1e-37 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 143 3e-35 gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 141 7e-35 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 595 bits (1534), Expect = e-171, Method: Compositional matrix adjust. Identities = 289/336 (86%), Positives = 312/336 (92%), Gaps = 3/336 (0%) Query: 23 QAQTSVTVYGLIDTGVEYLNNA--NAAGNSVVRLSSGNMNTSRIGFRGSEDLGGGLKAIF 80 QAQ+SVT+YGL+DTGVEYLNNA N AG SV R++SG NTSRIGFRG+EDLGGGLKA+F Sbjct: 20 QAQSSVTIYGLVDTGVEYLNNAGPNGAG-SVTRMTSGATNTSRIGFRGTEDLGGGLKALF 78 Query: 81 QLENGFKLDTGGFDGDANQIFNRQSNVGLEGGFGRVVAGRSYSTTYDFIGPFDPMGYSAN 140 QLENGFKLD G FDGDANQ+FNRQS VGLEGGFGRVVAGRSYSTTYDF+GPFDPMGYSAN Sbjct: 79 QLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGYSAN 138 Query: 141 YSWVTSAGATGGRKDGMPTGVSNLVKYQGEFSGVKIGAMYGFGEVAGQTSNGAKYGVGLG 200 YSW T+AGATG RKDGMPTGVSNLVKYQGEFSGVKIGAMYGFGEVAG+T NGAKYGVGLG Sbjct: 139 YSWATTAGATGNRKDGMPTGVSNLVKYQGEFSGVKIGAMYGFGEVAGETRNGAKYGVGLG 198 Query: 201 WGSGPFSLAATYDRNNSTTTVNTAYDRATSAHLAAGYQLADAWKFNLGYRYYKKTLASGA 260 WG+GPFS+ ATYDRNNS T NT YDRATSAHLAAGYQ ++AWKFNLGYRYYKK+LA+GA Sbjct: 199 WGTGPFSVVATYDRNNSATATNTPYDRATSAHLAAGYQPSEAWKFNLGYRYYKKSLATGA 258 Query: 261 TDLRSDFYWGGVSYKFTPAMTVIGAIYYQNIKNVAKDADPIMYSLRLKYAMSKRTDLYLS 320 DLRSDF+WGG +YKFTPAMT+IGAIYYQNIKNVA DADPIMYSLRLKYAMSKRTDLYLS Sbjct: 259 ADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDADPIMYSLRLKYAMSKRTDLYLS 318 Query: 321 TAYAKAKNNQLVGLSRDDVGFGNSQFGMTMGMQHRF 356 TAYAKAKN QLVGLSRDD G+G+SQFGMT+GMQHRF Sbjct: 319 TAYAKAKNGQLVGLSRDDAGYGSSQFGMTLGMQHRF 354 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 180 bits (457), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 135/370 (36%), Positives = 196/370 (52%), Gaps = 49/370 (13%) Query: 22 AQAQTSVTVYGLIDTGVEYLNNANAAGNSVVRLSSGNMNTSRIGFRGSEDLGGGLKAIFQ 81 A AQ++VT+YG++DT + YL+NANA GNS VR+ +G + SRIGF+G+EDLGGGLKA+FQ Sbjct: 19 AFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSRIGFKGTEDLGGGLKAMFQ 78 Query: 82 LENGFKLDTGGFDGDANQIFNRQSNVGLEGGFGRVVAGRSYSTTYDFIGP-FDPM--GYS 138 LENGF DTG +F RQS VGL+GGFG+V GR + +D + FDP+ G Sbjct: 79 LENGFSSDTGAMQ-QGGSLFGRQSWVGLQGGFGKVRVGRQNTPLFDLLADHFDPLTVGNY 137 Query: 139 ANYSWVTSAGATGGRKDGMPTGVSNLVKYQGEFSGVKIGAMYGFGEVAGQTSNGAKYGVG 198 A+ SW+ +A + N+++Y G+F V A + FGE AG G+++ Sbjct: 138 ASNSWLPAAASL--------VRSPNMLRYDGDFGPVAASASWAFGEKAGSVRTGSQFSSA 189 Query: 199 LGWGSGPFSLAATYDRNNSTTTVNTAYDRATSAHLAAGYQLADAWKFNLGYRYYKK---- 254 L + +GP +L Y + S+T + + T+ +L+A Y A F G+ K Sbjct: 190 LRYTAGPLALGGGYQQTVSSTNSSLKH---TTWNLSASYDFGVAKVFG-GWLNIKDDSGL 245 Query: 255 ---------------TLASGATD------LRSDFYWGGVSYKFTPAMTVIGAIYYQNIKN 293 T A A + + + ++ G SYK T A+T+ GA YY KN Sbjct: 246 TGSVMAADGDVSNGWTTAGAANNGSTGVKRKDNGFFLGSSYKATNALTLTGAFYYDRSKN 305 Query: 294 VA-----KDADPIMYSLR--LKYAMSKRTDLYLSTAYAKAKNNQLVGLSRDDVGFGNSQF 346 V+ D Y+L YA+SKRT LY + Y KAK+ + L+ G NS Sbjct: 306 VSYVSTGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNKAKDAAVYELTNSANG-KNSLT 364 Query: 347 GMTMGMQHRF 356 + +G++H F Sbjct: 365 TVGVGIRHIF 374 >gi|300313291|ref|YP_003777383.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 365 Score = 151 bits (381), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 123/353 (34%), Positives = 173/353 (49%), Gaps = 32/353 (9%) Query: 25 QTSVTVYGLIDTGVEYLNNANAAGNSVVRLSSGNMN-TSRIGFRGSEDLGGGLKAIFQLE 83 Q+SVT+YG+IDTGV Y NANA GNSV ++ S + SRIGF+G+EDLGGGL+A+F LE Sbjct: 24 QSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFPSRIGFKGTEDLGGGLQAMFVLE 83 Query: 84 NGFKLDTGGFDGDANQIFNRQSNVGLEGGFGRVVAGRSYSTTYDFIGPFDPMGYSANYSW 143 +GF DTG G ++F RQS VGL+ +G+++ GR + TY D MG + Sbjct: 84 SGFAPDTGAM-GQGGRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQLKTDVMGPNLFAIG 142 Query: 144 VTSAGATGGRKDGMPTGVSNLVKYQGEFSGVKIGAMYGF----------------GEVAG 187 A R D N + Y G FSG +GA Y F GEVAG Sbjct: 143 SIDAYLPNARSD-------NAIGYLGNFSGFTVGATYSFGRDAAGPAGPAATNCPGEVAG 195 Query: 188 QTSNGAKYGVGLGWGSGPFSLAATYDR---NNSTTTVNTAYDRATSAHLAAGYQLADAWK 244 + + LG+ + + ++YD N + T+ D GY + K Sbjct: 196 NSKACRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTDQRVTLNGYFMLGDVK 255 Query: 245 FNLGYRYYKKTLASGATDLRSDFYWGGVSYKFTPAMTVIGAIYYQNIKNVAKDADPIMYS 304 G ++T A+ T+ SD Y+ GVSY + A+ + + ++KN D + Sbjct: 256 IGGGV-VDRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLDVKNSPNDV--TLSV 312 Query: 305 LRLKYAMSKRTDLYLSTAYAKAKNNQLVGLSR-DDVGFGNSQFGMTMGMQHRF 356 RL Y +SKRT +Y S Y K V L VG G +Q G+ G++H F Sbjct: 313 ARLTYNLSKRTAVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLGVMTGIRHTF 365 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 143 bits (360), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 128/385 (33%), Positives = 181/385 (47%), Gaps = 70/385 (18%) Query: 26 TSVTVYGLIDTGVEYLNN-ANAAGN--SVVRLSSGNMNTSRIGFRGSEDLGGGLKAIFQL 82 +SVT+YG++DTGV Y + A G S L+SG + SRIGF+G+EDLGGGL A+F L Sbjct: 22 SSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQGSRIGFKGTEDLGGGLSAVFNL 81 Query: 83 ENGFKLDTGGFDGD--ANQIFNRQSNVGLEGGFGRVVAGR---------SYSTTYDFIGP 131 E GF DTG D +N +F R+S VGL G FG V+ GR SY++ DF G Sbjct: 82 EAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLLGRQTDFADTISSYTSVQDFGG- 140 Query: 132 FDPMGYSANYSWVTSAGATGGRKDGMPTGVSNLVKYQ-GEFSGVKIGAMYGFGEVAGQTS 190 W +AG R +G T +N + Y +G MYGFGE AGQTS Sbjct: 141 -----------WTGAAGHNLDRLEGTRT--NNSISYTTANLNGFTGNLMYGFGETAGQTS 187 Query: 191 NGAKYGVGLGWGSGPF------------------SLAATYDRNNSTTTVNTAYDRATSAH 232 G +GVG + +GP SL A N T N + Sbjct: 188 AGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQFTNGTNNGSTAQKVLN 247 Query: 233 LAAGYQLADAWKFNLGYRYYKKTL-------ASGATDL------------RSDFYWGGVS 273 +AA YQ A + + K+ L A+G L ++D Y G + Sbjct: 248 VAASYQFGPARVYG-NWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKADIYEIGTA 306 Query: 274 YKFTPAMTVIGAIYYQNIK--NVAKDADPIMYSLRLKYAMSKRTDLYLSTAYAKAKNNQL 331 Y +P++ ++ A+ + + V+ A SL Y +SKRTDLY AY+KA + Sbjct: 307 YSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDLYAFLAYSKATDLNN 366 Query: 332 VGLSRDDVGFGNSQFGMTMGMQHRF 356 G++ D G +Q + +G++H+F Sbjct: 367 PGVA-DSSGTDGNQGAVAVGIRHKF 390 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 141 bits (356), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 122/376 (32%), Positives = 179/376 (47%), Gaps = 55/376 (14%) Query: 26 TSVTVYGLIDTGVEYLN-------NANAAGNSVVRLSSGNMNTSRIGFRGSEDLGGGLKA 78 +SVT+YG++DTG+ Y + N NAA S ++SG + SRIGF+G EDLGGGL A Sbjct: 22 SSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSGVIQGSRIGFKGVEDLGGGLSA 81 Query: 79 IFQLENGFKLDTGGFDGD----ANQIFNRQSNVGLEGGFGRVVAGRSYSTTYDFIGPFDP 134 +F LE GF D GG G ++ +F R+S VGL GGFG V+ GR DF Sbjct: 82 VFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGFGTVLLGRQ----TDFADTISA 137 Query: 135 MGYSANYSWVTS-AGATGGRKDGMPTGVSNLVKY-QGEFSGVKIGAMYGFGEVAGQTSNG 192 A++ V + +G++ R G T +N V Y SG +YGFGE AG+TS G Sbjct: 138 YTAVADFGGVVANSGSSLNRLQGTRT--NNSVSYTTANLSGFTGNLIYGFGEQAGKTSAG 195 Query: 193 AKYGVGLGWGSGPFSLAATYDRNNSTTTV----------------------NTAYDRATS 230 YG+G + +GP L Y ++ + +T NTA S Sbjct: 196 QAYGIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANTALGVAGNTALK---S 252 Query: 231 AHLAAGYQLADAWKFNLGYRYYKKTL--------ASGATDLRSDFYWGGVSYKFTPAMTV 282 ++ A YQ A + Y K+ L A+ A ++D Y G +Y +P++ + Sbjct: 253 LNVVASYQFGPARVY-ANYSRVKQDLKGTPTVATATLANAQKADTYEIGTAYSLSPSLKL 311 Query: 283 IGAIYYQ--NIKNVAKDADPIMYSLRLKYAMSKRTDLYLSTAYAKAKNNQLVGLSRDDVG 340 + A+ + V SL Y +SKRTDLY + +A + Q G++ G Sbjct: 312 LAAVDHSRATFNGVNTKGKLTQISLGADYFLSKRTDLYAFVSNMRASDIQNPGVTGGATG 371 Query: 341 FGNSQFGMTMGMQHRF 356 SQ + G++H+F Sbjct: 372 SDASQTAIITGIRHKF 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,120,864 Number of extensions: 51393 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 14 Length of query: 356 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 270 Effective length of database: 1,212,660 Effective search space: 327418200 Effective search space used: 327418200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)