BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4393.p (315 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillu... 508 e-145 gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herb... 239 2e-64 gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane h... 96 3e-21 >gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillum seropedicae SmR1] Length = 315 Score = 508 bits (1307), Expect = e-145, Method: Compositional matrix adjust. Identities = 249/315 (79%), Positives = 275/315 (87%) Query: 1 MPDQPSRRHFLRRSIRFAGALGAATYMPIALAQNVSYPHSAGDALPVARLPANACDCHMH 60 M D PSRR FLRRS + A +LGAA ++P ALAQ +YPHS+G +RLP +ACDCHMH Sbjct: 1 MQDLPSRRDFLRRSGQLALSLGAAMHLPHALAQADAYPHSSGSQRAASRLPDDACDCHMH 60 Query: 61 IYDARFPWAPGAKLLHPPATVAMYRQIQQRLGSTRNVVVTPSAYGVDNRCTLDALAQFGA 120 IYD RF WAPGAKL+H PATVAMYRQ+QQRLG+TRNVVVTPSAYGVDN CTLDALAQ G Sbjct: 61 IYDPRFAWAPGAKLVHAPATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGR 120 Query: 121 TARGVAVVNDQVSDEELQRLHQAGVRGLRFNLALGSVTTVAMMEPLARRVAALGWHLQVN 180 +ARGVAVVNDQVSD ELQRLHQAGVRGLRFNLA+GSVTTV MMEPLARR+A LGWHLQ N Sbjct: 121 SARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVTTVEMMEPLARRMAPLGWHLQAN 180 Query: 181 MSNEVLLANGALLARLAVPVVFDHFARIPLQAGAGDPVFDLVTSLMREQRASVKLSGAYL 240 M NEVLLA+ A+LARL VP+VFDHFARIPLQA A PVFD VT LMRE+RASVKLSGAYL Sbjct: 181 MPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRERRASVKLSGAYL 240 Query: 241 FSKRGAPAYEDVAPLARALVEVAPTQVVWGSDWPHPTEQHKPDDARLLDALIGWLGSEQM 300 +SKRGAP Y+DVAPLARALV++APTQ+VWGSDWPHPTEQHKPDDARLLD +I WLGSE+M Sbjct: 241 YSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARLLDVMISWLGSEEM 300 Query: 301 IHTVLVDNPARLYHF 315 + TVLV NPARLY F Sbjct: 301 VRTVLVANPARLYQF 315 >gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 239 bits (610), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 141/308 (45%), Positives = 180/308 (58%), Gaps = 7/308 (2%) Query: 12 RRSIRFAGALGAATYMPIALAQNVSYPHSAGDALPVARLPANACDCHMHIYDARFPWAPG 71 RRS AGA+ A + A AQ+V P+SAG P + P ACD H HIYDARFP +P Sbjct: 4 RRSF-IAGAMLACAGVRSATAQSV--PNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPH 60 Query: 72 AKLLHPP-ATVAMYRQIQQRLGSTRNVVVTPSAYGVDNRCTLDALAQFGATARGVAVVND 130 + P ATVA YR + +LG R+VVV PS YG DNRC LDAL QFGA ARG+ V++D Sbjct: 61 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 120 Query: 131 QVSDEELQRLHQAGVRGLRFNLALG---SVTTVAMMEPLARRVAALGWHLQVNMSNEVLL 187 V +L+ + + GVRG+R N VTTV + A R+A LGWH+QV MS + Sbjct: 121 DVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIA 180 Query: 188 ANGALLARLAVPVVFDHFARIPLQAGAGDPVFDLVTSLMREQRASVKLSGAYLFSKRGAP 247 + A+LA L VV DH RIP G P V L+ + +KLS Y + +G P Sbjct: 181 QHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPP 240 Query: 248 AYEDVAPLARALVEVAPTQVVWGSDWPHPTEQHKPDDARLLDALIGWLGSEQMIHTVLVD 307 Y D + LA+A ++ AP + +WGSDWPHPTE+ KPDDA L D L W + +LV Sbjct: 241 DYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVS 300 Query: 308 NPARLYHF 315 NPA L+ F Sbjct: 301 NPAALFGF 308 >gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 96.3 bits (238), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 79/267 (29%), Positives = 118/267 (44%), Gaps = 13/267 (4%) Query: 55 CDCHMHIYDARFPWAPGAKLLHPPATVAMYRQIQQRL---GSTRNVVVTPSAYGVDNRCT 111 D H H++ P A + + P Q RL G + V+V PS G DNR Sbjct: 14 IDTHAHVFTRDLPLADVRR--YAPTYDVTVEQYIARLDTHGMSHGVLVQPSFLGSDNRYL 71 Query: 112 LDALAQFGATARGVAVVNDQVSDEELQRLHQAGVRGLRFNLALGSVT---TVAMMEPLAR 168 L ALA++ RG+A+V+ VS+++L L GV G+RFNL G+ T LA Sbjct: 72 LAALARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFTGVWRSTLA- 130 Query: 169 RVAALGWHLQVNMSNEVLLANGALLARLAVPVVFDHFARIPLQAGAGDPVFDLVTSLMRE 228 R+A LGW ++++ L L + + VV DHF R+ G DP F + S Sbjct: 131 RIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPGFRYLLSSAAG 190 Query: 229 QRASVKLSGAYLF--SKRGAPAYEDVAPLARALVEVAPTQVVWGSDWPHPTEQHKPDDAR 286 + VKLS +Y +++G PL R +++WGSDWPH + D Sbjct: 191 GQVWVKLSASYRNGGAEQGQRFARQAWPLLRE--HFGAQRLLWGSDWPHTQHESLTDYGH 248 Query: 287 LLDALIGWLGSEQMIHTVLVDNPARLY 313 + E+ + A+L+ Sbjct: 249 SWQQFTQLVSDEEQRRIITGQTAAQLF 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,028,283 Number of extensions: 43808 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 148 Number of HSP's successfully gapped: 3 Length of query: 315 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 230 Effective length of database: 1,217,395 Effective search space: 280000850 Effective search space used: 280000850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)