BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4392.p (446 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 804 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 351 7e-98 gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 332 4e-92 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 323 2e-89 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 296 3e-81 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 804 bits (2077), Expect = 0.0, Method: Compositional matrix adjust. Identities = 413/446 (92%), Positives = 432/446 (96%), Gaps = 1/446 (0%) Query: 1 MSQASINFIASAPVSTAAVSEQATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKE 60 MSQASI+ A++ V+TAAVSE ATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNK+ Sbjct: 1 MSQASIS-PAASAVATAAVSEDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKD 59 Query: 61 LGLTPSQFGLGAGLFFIGYFFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPK 120 LGLTPSQFGLGAGLFFIGYFFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPK Sbjct: 60 LGLTPSQFGLGAGLFFIGYFFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPK 119 Query: 121 SFALARFLLGMAEAGFTPGIYLYFTQWFPGPWRAKATAFFLIGIPVANIIGSPLSGALME 180 SFALARFLLGMAEAGFTPGIYLYFTQWFPG WR KATAFFLIGIPVANIIGSPLSGALME Sbjct: 120 SFALARFLLGMAEAGFTPGIYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME 179 Query: 181 LHGMWGFQGWQVLLLLEALPAVLLGVLCLFLLPDRPAKANWLNPAEKQWLENELSTEQNV 240 LHGMWGF+GWQVLLLLEALPAVLLGV+CLFLLPDRPAKA WL+ EKQWLENELSTEQNV Sbjct: 180 LHGMWGFKGWQVLLLLEALPAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNV 239 Query: 241 LAQRHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIKEFGLPAHQVGLVAALP 300 LA RHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQII+EFGLP+HQVGLVAA+P Sbjct: 240 LAARHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIP 299 Query: 301 YVVGAIAMTLWARLANRSQRRLLFVAGAIVLAAVSLAVSAFLHTPLLKMLAITVAVASIL 360 Y+VGA+AMTLWARLANRS+RRL FVAGAIVLAA+SL +SAFLHTPLLKMLAITVAVASIL Sbjct: 300 YLVGAVAMTLWARLANRSERRLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASIL 359 Query: 361 SFQATFWAIPSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLLFVAG 420 SFQATFWAIPSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPL+FVAG Sbjct: 360 SFQATFWAIPSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAG 419 Query: 421 ALLLGAVITLALGDPARVHSTSKETS 446 ALLLGAVITLALGDP+RV TSKET+ Sbjct: 420 ALLLGAVITLALGDPSRVPPTSKETA 445 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 351 bits (900), Expect = 7e-98, Method: Compositional matrix adjust. Identities = 189/421 (44%), Positives = 273/421 (64%), Gaps = 8/421 (1%) Query: 21 EQATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKELGLTPSQFGLGAGLFFIGYF 80 E AT KV RL+P + LCYV SYLDR+NVGFA L M ++L + + +GLGAG+FF+GYF Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 81 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 140 FE+PSN+ LH+ GAR+WI+RIMI+WG+IS A A+V P+ F + RFLLG+AEAGF PG+ Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 141 YLYFTQWFPGPWRAKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFQGWQVLLLLEAL 199 LY T W+P R K TA F+ GI ++ +IG PLSG +M + G+WG GWQ + +LEA+ Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 200 PAVLLGVLCLFLLPDRPAKANWLNPAEKQWLENELSTEQNVLAQRHGNKLKDAFTNWRVF 259 P+++LGV+ + + DR A+WL+ EK+ LE +S E++ Q+ L F N RV+ Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEES---QKEHLSLGAMFANGRVW 253 Query: 260 ALAGANFCGIIGSLSIGLWLPQIIKEFGLPAH-QVGLVAALPYVVGAIAMTLWARLANRS 318 A FC ++G + WLP IIK G+ VGL+ A+PY A AM L R A+ Sbjct: 254 LGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADAR 313 Query: 319 QRRLLFVAGAIVLAAVSLAVSA-FLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGR 377 + R VA + + L +S + H L M ++T+A IL+ FW++P+ FL+G Sbjct: 314 RERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGT 373 Query: 378 AAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLLFVAGALLLGAVITLALGDPAR 437 AAA G+ALI S+GNL GFV P ++G++++ATQ + +A +L++G ++TL L PA+ Sbjct: 374 AAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTL--PAK 431 Query: 438 V 438 + Sbjct: 432 L 432 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 332 bits (851), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 171/418 (40%), Positives = 258/418 (61%), Gaps = 1/418 (0%) Query: 21 EQATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKELGLTPSQFGLGAGLFFIGYF 80 EQ T++KV RL+PF++LCY+ +++DR N+G AAL MN +LGL+P FG + LFFI YF Sbjct: 11 EQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYF 70 Query: 81 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 140 FE+PSNLAL +FGAR W++RIMI+WGLIS TA V GP S RF+LG AEAGF PG+ Sbjct: 71 IFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILGAAEAGFFPGV 130 Query: 141 YLYFTQWFPGPWRAKATAFFLIGIPVANIIGSPLSGALMELHGMWGFQGWQVLLLLEALP 200 LY T W P +RA+ A F++ IP A+ + SP+S L+++ G+ G +GW L +LE LP Sbjct: 131 VLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFMLEGLP 190 Query: 201 AVLLGVLCLFLLPDRPAKANWLNPAEKQWLENELSTEQNVLAQRHGNKLKDAFTNWRVFA 260 V+LG++CL L DRP +A WL+ ++QWL ++ E A + L F+ V+ Sbjct: 191 TVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHVWG 250 Query: 261 LAGANFCGIIGSLSIGLWLPQIIKEFGLPAHQVGLVAALPYVVGAIAMTLWARLANRSQR 320 +A C + +W PQ++K FGL Q GLV ++PY++ ++ M W R ++R+ Sbjct: 251 MALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVWWGRHSDRTGE 310 Query: 321 RLLFVAGAIVLAAVSLAVSAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRAAA 380 R A ++L LA+++ ++ +L +T+ + SF+ FWA+ S +L +AA Sbjct: 311 RRWHTAIPLMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFKGPFWAMASSWLATGSAA 370 Query: 381 GGLALIVSIGNL-GGFVGPSVIGFIREATQGFTYPLLFVAGALLLGAVITLALGDPAR 437 GLA I ++ NL GG + +V G++++AT + LL + G ++ I L L +R Sbjct: 371 AGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGILLFLSRKSR 428 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 323 bits (828), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 179/417 (42%), Positives = 268/417 (64%), Gaps = 9/417 (2%) Query: 20 SEQATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKELGLTPSQFGLGAGLFFIGY 79 S+ KKV R++PF++LCYV++YLDR+NVGFA L M+++LG + + FGLGAGLFFIGY Sbjct: 17 SKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGY 76 Query: 80 FFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPG 139 F FE+PSN+ +H+ GAR+WI+RIMI+WG++S A FV F + RFLLG+AEAGF PG Sbjct: 77 FLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPG 136 Query: 140 IYLYFTQWFPGPWRAKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFQGWQVLLLLEA 198 I LY T WFP RAK A F+ GIPVA I+G+PLSG +M+ H G +GWQ + L+EA Sbjct: 137 IILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEA 196 Query: 199 LPAVLLGVLCLFLLPDRPAKANWLNPAEKQWLENELSTEQNVLAQRH--GNKLKDAFTNW 256 +PAVL+GV + L + A WLN EK L+ ++ + +H G +KDA Sbjct: 197 IPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDA---- 252 Query: 257 RVFALAGANFCGIIGSLSIGLWLPQIIKEFGLPAH-QVGLVAALPYVVGAIAMTLWARLA 315 RV+ + F ++G + LW+P ++K G+ + ++GL++A+P+ IAM L R A Sbjct: 253 RVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSA 312 Query: 316 NR-SQRRLLFVAGAIVLAAVSLAVSAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFL 374 +R +RR V A++ + + F + + ++++A A +L+ FW++P+ FL Sbjct: 313 DRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFL 372 Query: 375 TGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLLFVAGALLLGAVITLA 431 +G AAA G+A I S+GNL GFV P +IG++++ T + +A L++G++ TLA Sbjct: 373 SGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLA 429 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 296 bits (758), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 171/401 (42%), Positives = 249/401 (62%), Gaps = 5/401 (1%) Query: 22 QATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKELGLTPSQFGLGAGLFFIGYFF 81 + T +K+ RLIPF++ +V++++DR+NVGFA L M ++L + + +GLGAG+FFIGYF Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 82 FEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGIY 141 FE+PSNL L + GAR ++RI I WGL SMA A+V P SF + RFLLG+ EAGF PG+ Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 142 LYFTQWFPGPWRAKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFQGWQVLLLLEALP 200 LY T WFP RA+ F+ +A +G P++G +M + G+ WQ L +LE +P Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 201 AVLLGVLCLFLLPDRPAKANWLNPAEKQWLENELSTEQNVLAQRHGNKLKDAFTNWRVFA 260 ++L G L LP+RPA A WL+ AEK + L+ E + + KDA TN+RV+ Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHF--SFKDACTNYRVWI 259 Query: 261 LAGANFCGIIGSLSIGLWLPQIIKEFGLPAH-QVGLVAALPYVVGAIAMTLWARLANRSQ 319 A FC + G+ +I W P IIKE G+ + Q+GL++A+P++ G +AM ++++ Sbjct: 260 CAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTG 319 Query: 320 RRLLFVAGAIVLAAVSLAVSAF-LHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRA 378 R L A A ++A + L + L L + ++T+A IL+ FW+IP FL G A Sbjct: 320 ERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTA 379 Query: 379 AAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLLFVA 419 AAGG+ALI SIGNL GFV P +IG ++ +T + L FVA Sbjct: 380 AAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,223,003 Number of extensions: 48261 Number of successful extensions: 331 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 288 Number of HSP's successfully gapped: 21 Length of query: 446 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 358 Effective length of database: 1,203,190 Effective search space: 430742020 Effective search space used: 430742020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)