BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4388.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 795 0.0 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 343 2e-95 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 311 9e-86 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 296 2e-81 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 291 5e-80 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 795 bits (2052), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/428 (91%), Positives = 412/428 (96%) Query: 1 METIQDEKRLERETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGF 60 METIQ+EKRLE+ETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGF Sbjct: 1 METIQNEKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGF 60 Query: 61 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIMITWGLISASTALVQGPHSLYVARFILG 120 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIMITWGLISA TALVQGP+SLY RFILG Sbjct: 61 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILG 120 Query: 121 AAEAGFFPGVVLYLTYWVPSAYRARMVALFMVAIPAASFVASPVSALLLQMDGIGGLRGW 180 AAEAGFFPGVVLYLTYWVPSAYRAR+VALFMVAIPAASFVASPVSALLLQMDGIGGLRGW Sbjct: 121 AAEAGFFPGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGW 180 Query: 181 HWLFVLEGLPTVLLGVVCLMFLTDRPEQARWLDEHQRNWLVATMNREAASRAAKQHIPLW 240 HWLF+LEGLPTV+LG+VCL FLTDRP+QARWL + QR WL TMN+EAASRAAKQHIPLW Sbjct: 181 HWLFMLEGLPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLW 240 Query: 241 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVW 300 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVW Sbjct: 241 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVW 300 Query: 301 WGRHSDRSGERRWHTAIPLMLIGGGLACATLIHSLIPVVLMLTLVLIGAYCFKGPFWAMA 360 WGRHSDR+GERRWHTAIPLMLIGGGLA A+L +SL PVVL+LT+VLIGAY FKGPFWAMA Sbjct: 301 WGRHSDRTGERRWHTAIPLMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFKGPFWAMA 360 Query: 361 TNWLATGSVAAGLAGINAVSNLIGGGLMVNVYGWVKGATGSFALALLPLVGLSVLSVAIL 420 ++WLATGS AAGLAGINAVSNLIGGGLMVNVYGWVK ATGS+A+ALLP+VGLSV+SV IL Sbjct: 361 SSWLATGSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGIL 420 Query: 421 LFLSRKSR 428 LFLSRKSR Sbjct: 421 LFLSRKSR 428 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 343 bits (880), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 177/423 (41%), Positives = 259/423 (61%), Gaps = 1/423 (0%) Query: 11 ERETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYF 70 E T++KV RL+PF++LCY+ +++DR N+G AAL MN DLGL+P FG + LFFI YF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 71 IFEVPSNLALQKFGARKWLARIMITWGLISASTALVQGPHSLYVARFILGAAEAGFFPGV 130 FE+PSNLAL +FGAR W++RIMI+WGLIS +TA V GP S +ARF+LG AEAGF PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 131 VLYLTYWVPSAYRARMVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFVLEGLP 190 LY T W P A+R + A F++ IP A+ + SP+S L+++ G+ G +GW L +LE LP Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 199 Query: 191 TVLLGVVCLMFLTDRPEQARWLDEHQRNWLVATMNREAASRAAKQHIPLWKLFSQPHVWG 250 VLLGV+CL L DRP +A+WL ++ WL ++ E AA+ L F+ V+ Sbjct: 200 AVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA 259 Query: 251 MALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVWWGRHSDRSGE 310 +A C + +W PQ+++ FGL Q GLV +IPYL+ +V M W R ++RS Sbjct: 260 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRSER 319 Query: 311 RRWHTAIPLMLIGGGLACATLIHSLIPVVLMLTLVLIGAYCFKGPFWAMATNWLATGSVA 370 R + A ++L L + +H+ + +L +T+ + F+ FWA+ + +L + A Sbjct: 320 RLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRAAA 379 Query: 371 AGLAGINAVSNLIGGGLMVNVYGWVKGATGSFALALLPLVGLSVLSVAILLFLSRKSRTP 430 GLA I ++ NL GG + +V G+++ AT F L+ + G +L I L L SR P Sbjct: 380 GGLALIVSIGNL-GGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLALGDPSRVP 438 Query: 431 AAT 433 + Sbjct: 439 PTS 441 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 311 bits (796), Expect = 9e-86, Method: Compositional matrix adjust. Identities = 167/395 (42%), Positives = 242/395 (61%), Gaps = 7/395 (1%) Query: 10 LERETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAY 69 E T KVTWRLLP L LCY+ +++DR N+G A LQM DL S ++G + +FF+ Y Sbjct: 16 FEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGY 75 Query: 70 FIFEVPSNLALQKFGARKWLARIMITWGLISASTALVQGPHSLYVARFILGAAEAGFFPG 129 F+FEVPSN+ L K GAR W+ARIMITWG+IS + A V P Y+ RF+LG AEAGFFPG Sbjct: 76 FVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPG 135 Query: 130 VVLYLTYWVPSAYRARMVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFVLEG 188 V+LYLTYW P+A R ++ ALFM I + + P+S ++ M G+ G GW W+F+LE Sbjct: 136 VILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEA 195 Query: 189 LPTVLLGVVCLMFLTDRPEQARWLDEHQRNWLVATMNREAASRAAKQHIPLWKLFSQPHV 248 +P+++LGV+ ++++ DR A WL E ++ L A ++ E + K+H+ L +F+ V Sbjct: 196 IPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHE---ESQKEHLSLGAMFANGRV 252 Query: 249 WGMALVCACASAAGSVLNVWQPQLLKSFGLSV-MQTGLVNSIPYLIASVLMVWWGRHSDR 307 W AL+ C ++ W P ++K+ G+S + GL+ +IPY A+ MV GR +D Sbjct: 253 WLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADA 312 Query: 308 SGERRWHTAIPLMLIGGGLACATLI-HSLIPVVLMLTLVLIGAYCFKGPFWAMATNWLAT 366 ERRWH AIP + GL +T H+ + LTL +G FW++ T +L+ Sbjct: 313 RRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSG 372 Query: 367 GSVAAGLAGINAVSNLIGGGLMVNVYGWVKGATGS 401 + AAG+A IN++ NL G + + GW+K AT S Sbjct: 373 TAAAAGIALINSLGNL-AGFVSPFLVGWLKDATQS 406 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 296 bits (758), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 165/406 (40%), Positives = 248/406 (61%), Gaps = 15/406 (3%) Query: 16 RKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYFIFEVP 75 +KV WR++PFLMLCY+ A++DR N+G A LQM+ DLG S +FG + LFFI YF+FEVP Sbjct: 23 KKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVP 82 Query: 76 SNLALQKFGARKWLARIMITWGLISASTALVQGPHSLYVARFILGAAEAGFFPGVVLYLT 135 SN+ + K GAR W+ARIMITWG++SA+ VQ Y+ RF+LG AEAGF+PG++LYLT Sbjct: 83 SNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLT 142 Query: 136 YWVPSAYRARMVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFVLEGLPTVLL 194 YW PS RA+++A+FM IP A + +P+S ++ GGL GW W+F++E +P VL+ Sbjct: 143 YWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLI 202 Query: 195 GVVCLMFLTDRPEQARWLDEHQRNWLVATMNREAASRAAKQHIPLWKLFSQPHVWGMALV 254 GV +++L + + A+WL++ ++ L A ++ +A + +K + VW M L+ Sbjct: 203 GVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFG--AIVKDARVWLMCLI 260 Query: 255 CACASAAGSVLNVWQPQLLKSFGLS-VMQTGLVNSIPYLIASVLMVWWGRHSDRSGERRW 313 L +W P L+K+ G+ ++ GL+++IP+ A + M GR +DR ERRW Sbjct: 261 YFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERRW 320 Query: 314 HTAIPLMLIGGGLACATLIHSLIPV-VLMLTLVLIGAYCFKGPFWAMATNWLATGSVAAG 372 H IP ++ G G A L V + L+L G FW++ T +L+ + A G Sbjct: 321 HLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVG 380 Query: 373 LAGINAVSNLIGGGLMVNVY--GWVK-----GATGSFALALLPLVG 411 +A IN+V NL G V+ Y G++K ATG + LA++ +VG Sbjct: 381 IAAINSVGNLAG---FVSPYLIGYLKDLTHNNATGMYMLAVMLVVG 423 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 291 bits (746), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 168/416 (40%), Positives = 248/416 (59%), Gaps = 6/416 (1%) Query: 14 TVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYFIFE 73 T RK+ WRL+PFL+ ++ A+IDR N+G A LQM DL S ++G + +FFI YFIFE Sbjct: 24 TYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFE 83 Query: 74 VPSNLALQKFGARKWLARIMITWGLISASTALVQGPHSLYVARFILGAAEAGFFPGVVLY 133 VPSNL L+K GARK LARI I WGL S + A V P S Y+ RF+LG EAGFFPGVVLY Sbjct: 84 VPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLY 143 Query: 134 LTYWVPSAYRARMVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFVLEGLPTV 192 LTYW P+ RAR+ LFM + A V P++ L++ M+G+G L W WLF+LEG+P++ Sbjct: 144 LTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSI 203 Query: 193 LLGVVCLMFLTDRPEQARWLDEHQRNWLVATMNREAASRAAKQHIPLWKLFSQPHVWGMA 252 L G L +L +RP A+WL E ++N + + E + A +H + VW A Sbjct: 204 LAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAE--NSAPGKHFSFKDACTNYRVWICA 261 Query: 253 LVCACASAAGSVLNVWQPQLLKSFGLS-VMQTGLVNSIPYLIASVLMVWWGRHSDRSGER 311 V C + + + W P ++K G+ +Q GL+++IP++ ++ M+W G HSD++GER Sbjct: 262 AVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGER 321 Query: 312 RWHTAIPLMLIGGGL-ACATLIHSLIPVVLMLTLVLIGAYCFKGPFWAMATNWLATGSVA 370 R H+AI ++ GL A + + + LTL IG FW++ +LA + A Sbjct: 322 RLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAA 381 Query: 371 AGLAGINAVSNLIGGGLMVNVYGWVKGATGSFALALLPLVGLSVLSVAILLFLSRK 426 G+A IN++ NL G + + G +K +TGS + L + L +++ +++ RK Sbjct: 382 GGIALINSIGNL-AGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFIRK 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.138 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,286,431 Number of extensions: 53373 Number of successful extensions: 351 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 317 Number of HSP's successfully gapped: 20 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)