BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4386.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herb... 546 e-157 gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillu... 248 4e-67 gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane h... 121 6e-29 >gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 546 bits (1408), Expect = e-157, Method: Compositional matrix adjust. Identities = 262/308 (85%), Positives = 287/308 (93%) Query: 1 MLSRRHFLAGSLLACSGLRAALAQSVPNSAGQQPPRLTAPALSCDSHHHIYDARFPVSPH 60 ML RR F+AG++LAC+G+R+A AQSVPNSAG QPPRL AP L+CDSHHHIYDARFPVSPH Sbjct: 1 MLDRRSFIAGAMLACAGVRSATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPH 60 Query: 61 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALRQFGTRARGIVVIED 120 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDAL QFG +ARGIVVI+D Sbjct: 61 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 120 Query: 121 EVTMVQLQEMHQLGVRGVRVNFLSPQTWGITTVERLRRTAEKIAPLGWHVQVLMSGAQIA 180 +V + QL+EM +LGVRGVRVNFLS Q+WG+TTVERLRRTAE+IAPLGWHVQVLMSGAQIA Sbjct: 121 DVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIA 180 Query: 181 EHEAVLAGLPTPVVIDHLGRIPQPAGLDHPGAQAILRLLRQGHTWIKLSEAYADTLQGPP 240 +HEAVLA LPT VVIDHLGRIPQP GLDHPGAQA+LRLL +G+TWIKLSE YADTLQGPP Sbjct: 181 QHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPP 240 Query: 241 DYPDTSLLARAYVQAAPQRAIWGSDWPHPTEKIKPDDAVLFDLLSAWAPDAATREQILVH 300 DYPDTSLLA+AY+QAAPQR +WGSDWPHPTEKIKPDDAVLFDLL+AWAPD A R+QILV Sbjct: 241 DYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVS 300 Query: 301 NPAALFGF 308 NPAALFGF Sbjct: 301 NPAALFGF 308 >gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillum seropedicae SmR1] Length = 315 Score = 248 bits (634), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 145/314 (46%), Positives = 183/314 (58%), Gaps = 12/314 (3%) Query: 3 SRRHFL------AGSLLACSGLRAALAQS--VPNSAGQQPPRLTAPALSCDSHHHIYDAR 54 SRR FL A SL A L ALAQ+ P+S+G Q P +CD H HIYD R Sbjct: 6 SRRDFLRRSGQLALSLGAAMHLPHALAQADAYPHSSGSQRAASRLPDDACDCHMHIYDPR 65 Query: 55 FPVSPHWRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALRQFGTRARG 114 F +P + ATVA YR L +LG R+VVV PS YG DN C LDAL Q G ARG Sbjct: 66 FAWAPGAKLVHAP-ATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGRSARG 124 Query: 115 IVVIEDEVTMVQLQEMHQLGVRGVRVNFLSPQTWGITTVERLRRTAEKIAPLGWHVQVLM 174 + V+ D+V+ +LQ +HQ GVRG+R N +TTVE + A ++APLGWH+Q M Sbjct: 125 VAVVNDQVSDAELQRLHQAGVRGLRFNL---AVGSVTTVEMMEPLARRMAPLGWHLQANM 181 Query: 175 SGAQIAEHEAVLAGLPTPVVIDHLGRIPQPAGLDHPGAQAILRLLRQGHTWIKLSEAYAD 234 + H A+LA LP P+V DH RIP A HP + L+R+ +KLS AY Sbjct: 182 PNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRERRASVKLSGAYLY 241 Query: 235 TLQGPPDYPDTSLLARAYVQAAPQRAIWGSDWPHPTEKIKPDDAVLFDLLSAWAPDAATR 294 + +G PDY D + LARA VQ AP + +WGSDWPHPTE+ KPDDA L D++ +W Sbjct: 242 SKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARLLDVMISWLGSEEMV 301 Query: 295 EQILVHNPAALFGF 308 +LV NPA L+ F Sbjct: 302 RTVLVANPARLYQF 315 >gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 121 bits (304), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 89/283 (31%), Positives = 127/283 (44%), Gaps = 13/283 (4%) Query: 33 QPPRLTAPALSCDSHHHIYDARFPVSPHWRGGRPDGATVADYRLLMAKLGIARHVVVQPS 92 QP A L D+H H++ P++ R TV Y + G++ V+VQPS Sbjct: 3 QPCATPATVLRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQPS 62 Query: 93 TYGSDNRCLLDALRQFGTRARGIVVIEDEVTMVQLQEMHQLGVRGVRVNFLS----PQTW 148 GSDNR LL AL ++ R RGI +++ +V+ QL + GV G+R N + P Sbjct: 63 FLGSDNRYLLAALARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFT 122 Query: 149 GITTVERLRRTAEKIAPLGWHVQVLMSGAQIAEHEAVLAGLPTPVVIDHLGRIPQPAGLD 208 G+ R T +IA LGW V++ + L + VV+DH GR+ G+D Sbjct: 123 GV-----WRSTLARIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVD 177 Query: 209 HPGAQAILRLLRQGHTWIKLSEAYAD--TLQGPPDYPDTSLLARAYVQAAPQRAIWGSDW 266 PG + +L G W+KLS +Y + QG L R + A QR +WGSDW Sbjct: 178 DPGFRYLLSSAAGGQVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGA--QRLLWGSDW 235 Query: 267 PHPTEKIKPDDAVLFDLLSAWAPDAATREQILVHNPAALFGFT 309 PH + D + + D R I A LFG Sbjct: 236 PHTQHESLTDYGHSWQQFTQLVSDEEQRRIITGQTAAQLFGIN 278 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,132,712 Number of extensions: 52524 Number of successful extensions: 159 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 3 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)