BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4379.p (594 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 1213 0.0 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 435 e-123 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 419 e-118 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 192 9e-50 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 139 8e-34 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 1213 bits (3138), Expect = 0.0, Method: Compositional matrix adjust. Identities = 586/594 (98%), Positives = 590/594 (99%) Query: 1 MNKPNVTPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDIS 60 MNKPN TPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDIS Sbjct: 1 MNKPNATPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDIS 60 Query: 61 PCNRIHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYP 120 PCNRIHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYP Sbjct: 61 PCNRIHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYP 120 Query: 121 IDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLS 180 IDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLS Sbjct: 121 IDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLS 180 Query: 181 AGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMA 240 AGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMA Sbjct: 181 AGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMA 240 Query: 241 YETGRRIVGMAYEDLRPSAILTREAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELHSE 300 YETGRRIVGMAYEDLRPSAILTR+AFLDAIVVNAAIGGSTNAQPHIMAMARHAGVEL SE Sbjct: 241 YETGRRIVGMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSE 300 Query: 301 DWMKYGYDVPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITASGKTMAEN 360 DWMKYGYDVPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITA+GKTMAEN Sbjct: 301 DWMKYGYDVPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAEN 360 Query: 361 LQGRQSNDREMIYPFAAPLRERAGFLVLKGNLFDFAIMKTSVISEAFRERYLSTPGQENI 420 LQGR SNDREMIYPFAAPLRERAGFLVLKGNLFDFAIMKTSVISE FRERYLSTPGQENI Sbjct: 361 LQGRASNDREMIYPFAAPLRERAGFLVLKGNLFDFAIMKTSVISETFRERYLSTPGQENI 420 Query: 421 FECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRG 480 FECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRG Sbjct: 421 FECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRG 480 Query: 481 VSTLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLISEEE 540 VSTLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNML+SEEE Sbjct: 481 VSTLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEE 540 Query: 541 LARRKSEGIPPVPPSQTPWQEIYRSTVGQLETGACMELALKYQGVAQTMPRHNH 594 LARRKSEGIPPVPPSQTPWQEIYRSTVGQLETGACMELALKYQGVAQT+PRHNH Sbjct: 541 LARRKSEGIPPVPPSQTPWQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 594 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 435 bits (1119), Expect = e-123, Method: Compositional matrix adjust. Identities = 245/586 (41%), Positives = 356/586 (60%), Gaps = 21/586 (3%) Query: 12 RSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLELAK 71 RSQ WF D +Y N GI ++ GRP+IGI + S+++PCN LA+ Sbjct: 11 RSQAWFGRQDRDGF--IYRSWVKNRGIPHDQF-DGRPVIGICNTYSELTPCNSHFRALAE 67 Query: 72 RVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTTGCD 131 +V+ GI +AGG P+EFP+ + E RPTA + RNLA + + E + P+D VVL GCD Sbjct: 68 QVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVVLLMGCD 127 Query: 132 KTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEKFLE 191 KTTP+ +M AA+VD+P I +SGGPML G G +GSG+ +W+ + + AG + E+F E Sbjct: 128 KTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFE 187 Query: 192 IAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA 251 + S GHC TMGTASTM +M EALG+ L +AIPA R +A GRRIV M Sbjct: 188 AESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMV 247 Query: 252 YEDLRPSAILTREAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELHSEDWMKYGYDVPL 311 EDL S ILTR+AF +AI VNAAIGGSTNA H++A+A G++L +DW G +P Sbjct: 248 EEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQQLPC 307 Query: 312 LLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITASGKTMAENLQGRQSNDREM 371 LL++QP+GK+L E F+ AGG+PA++ +L+ + +TA+GKT+ EN Q + + E+ Sbjct: 308 LLDLQPSGKFLMEDFYYAGGLPAVIRQLESV--IDKTALTANGKTLWENCQDAPNWNEEV 365 Query: 372 IYPFAAPLRERAGFLVLKGNLF-DFAIMKTSVISEAFRERYLSTPGQENIFECRAVVFDG 430 I F P +E AG +LKGNL D A++K S + A + RAVVF+ Sbjct: 366 IRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKH-----------RGRAVVFEN 414 Query: 431 SDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLPTLGDG 490 SDD H RI+D L +DE +L ++ GP G+PG AE NM P ++++G++ + + D Sbjct: 415 SDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDA 474 Query: 491 RQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLISEEELARRKSE-GI 549 R SGT+ +L+ SPE+A GG LA +++GD + +D+ + ++ +S+EELARR+++ Sbjct: 475 RMSGTAYGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRRAQWQK 534 Query: 550 PPVPPS-QTPWQEIYRSTVGQLETGACMELALKYQGVAQTMPRHNH 594 P +PP Q W ++Y V Q GA ++ + G +P+ NH Sbjct: 535 PELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSG--PYVPKDNH 578 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 419 bits (1078), Expect = e-118, Method: Compositional matrix adjust. Identities = 245/594 (41%), Positives = 353/594 (59%), Gaps = 26/594 (4%) Query: 9 RRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLE 68 R RS WF D +Y N GI E + G+P+IGI + S+++PCN + Sbjct: 10 RTLRSAGWFGTADKNGF--MYRSWMKNQGIPDHEFQ-GKPVIGICNTWSELTPCNAHFRK 66 Query: 69 LAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTT 128 +A+ VR GI +AGG P+EFP+ E+ RPTA + RNLA + + E + G PIDAVVL T Sbjct: 67 IAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPIDAVVLLT 126 Query: 129 GCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEK 188 GCDKTTP+ +M AA+ D+PAIV++GGPML+G G +GSG+ +W+ + + AG I Sbjct: 127 GCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAGEITIHD 186 Query: 189 FLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIV 248 F+ A + S+G CNTMGTASTM MAE+LG+SL +AIPA R +A+ +G RIV Sbjct: 187 FMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIV 246 Query: 249 GMAYEDLRPSAILTREAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELHSEDWMKYGYD 308 M +E L S ILTR+AF +AI NAAIGGSTNA H+ A+A GV+L EDW + G Sbjct: 247 DMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRG 306 Query: 309 VPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKL-RAERITASGKTMAENLQGRQSN 367 P ++++QP+G+YL E F+ AGG+PA++ L +A L + +T +G+TM +N++ Sbjct: 307 TPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNVKDAPIY 366 Query: 368 DREMIYPFAAPLRERAGFLVLKGNLF-DFAIMKTSVIS-EAFRERYLSTPGQENIFECRA 425 + E++ P A PL E G +L+GNL A++K S + E + R RA Sbjct: 367 NDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHR------------GRA 414 Query: 426 VVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLP 485 VVF+ + Y RINDP L +D + +L ++ GP G+PG AEV NM P ++ GV + Sbjct: 415 VVFEDFNHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMV 474 Query: 486 TLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLISEEELARRK 545 + D R SGT+ IL+ +PE+A GG L ++DGD + +D G+ + ISEEE+ RR Sbjct: 475 RISDARMSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRL 534 Query: 546 SEGIPPVPPSQTP-----WQEIYRSTVGQLETGACMELALKYQGVAQTMPRHNH 594 E V + P +Q +Y V Q + G + + +G A +P+H+H Sbjct: 535 -EARAKVLAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAA--VPKHSH 585 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 192 bits (487), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 166/558 (29%), Positives = 268/558 (48%), Gaps = 75/558 (13%) Query: 47 RPIIGIAQSGSDISPCNRIHL-ELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAI-- 103 +PII + S + P +HL +L + V I AGG+ EF + ++ A + Sbjct: 34 KPIIAVVNSFTQFVP-GHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDSGTHGDARMLY 92 Query: 104 ---DRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGW 160 R+L + +++ + DA+V + CDK TP +MAA ++IP + +SGGPM G Sbjct: 93 SLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGK 152 Query: 161 MDGELVGSGSAIWK---GRKLLSAG--SIDNEKFLEIAAASAPSSGHCNTMGTASTMNAM 215 + + V + + K ++ AG +I + E+ ++ P+ G C+ M TA++MN + Sbjct: 153 VI-KTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCSGMFTANSMNCL 211 Query: 216 AEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA---YED----LRPSAILTREAFLD 268 E LG+SL G I A + +R ++ GR IV +A YE + P I T+E + + Sbjct: 212 TEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILPRNICTKETWEN 271 Query: 269 AIVVNAAIGGSTNAQPHIMAMARHAGVELHSEDWMKYGYDVPLLLNMQP-AGKYLGERFH 327 A+ ++ ++GGSTN H++A A A VE D + VP L + P KY E H Sbjct: 272 AMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRRVPCLCKVAPMTNKYHIEDVH 331 Query: 328 RAGGVPAIMWELQQAGKLRAERITASGKTMAENLQG---RQSNDREMIYPF-AAP----- 378 RAGG+ +I+ EL +AG L R T KT+ E ++ R S+D + F AAP Sbjct: 332 RAGGIISILGELARAGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHKLFRAAPGGVPT 391 Query: 379 ---------------------LRER-------AGFLVLKGNLFDF-AIMKTSVISEAFRE 409 +R+R G VL GN+ + I+KT+ + E+ Sbjct: 392 QVAFSQEERFEANDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESI-- 449 Query: 410 RYLSTPGQENIFECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVN 469 L G +FE + +G + D K+ ++ +R GP G PG E+ Sbjct: 450 --LKFSGTARVFESQDAAVEGI------LGD---KVKAGDVVIVRYEGPKGGPGMQEM-- 496 Query: 470 MQPPDALIKRGVSTLPTL-GDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLN 528 + P + +G+ L DGR SG S I +ASPE+A GG + + +GD + ID+ Sbjct: 497 LYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIP 556 Query: 529 TGECNMLISEEELARRKS 546 N+ IS ++LA+R++ Sbjct: 557 NRTINLRISSDDLAKRRT 574 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 139 bits (349), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 129/482 (26%), Positives = 207/482 (42%), Gaps = 51/482 (10%) Query: 121 IDAVVLTTGCDKTTPSQIMAAATV-DIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLL 179 DA + CDK P ++ A +PA+ + GPM G + + V K R+L Sbjct: 147 FDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSGMSNSDKV-------KIRQLY 199 Query: 180 SAGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIP--APYRERG 237 +AG I + LE + S +G C GTA++ + EA+G+ L G + I P R+ Sbjct: 200 TAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAMGLHLPGAAFITPNTPLRDAL 259 Query: 238 QMAYETGR-RIVGMAYEDLRPSAILTREAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVE 296 A +I + + ++ ++ ++AIV A GGSTN H++A+A+ AG+ Sbjct: 260 TAAAAKQAAKITDLGTQYTPVGRMIDEKSIVNAIVALHATGGSTNHTLHLVAIAKAAGIV 319 Query: 297 LHSEDWMKYGYDVPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITASGKT 356 + +D+ K VPL+ + P G FH AGG ++ EL AG + + T G+ Sbjct: 320 IDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFVLRELIDAGLMHDDVTTILGQG 379 Query: 357 M----------AENLQGR-----------QSNDREMIYPFAAPLRERAGFLVLKGNLFDF 395 + AE GR QS D ++ A P G +L+GNL Sbjct: 380 LRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEGVLGTVAKPFAADGGLKLLQGNL-GR 438 Query: 396 AIMKTSVISEAFRERYLSTPGQENIFECRAVVFDGSDDYHARINDPALKIDENTLLAIRG 455 A++K S + R + E AVVF + + A L D ++ +G Sbjct: 439 AVIKISAVKPEHR-----------VVEAPAVVFHSQEAFMAAFKAGQLDRDFVAVITFQG 487 Query: 456 AGPVGWPGSAEVVNMQPPDALIKRGVSTLPTLGDGRQSGTSDS-PSILNASPESAVGGGL 514 G P E+ + P +++ + + DGR SG S P+ ++ +PE GG L Sbjct: 488 PRANGMP---ELHALTPALGILQDKGRHVALVTDGRMSGASGKVPAAIHVTPEVLGGGPL 544 Query: 515 AYLRDGDRVRIDLNTGECNMLISEEELARRKSEGIPPVPPSQTPWQE---IYRSTVGQLE 571 +RDGD +R+D G + E A R + +E ++R+ VG E Sbjct: 545 GRVRDGDIIRLDAEAGTLEAKVDATEWAARSQDSSDLSGNQHGMGRELFSLFRNAVGTAE 604 Query: 572 TG 573 G Sbjct: 605 EG 606 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,991,378 Number of extensions: 90650 Number of successful extensions: 272 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 260 Number of HSP's successfully gapped: 6 Length of query: 594 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 503 Effective length of database: 1,188,985 Effective search space: 598059455 Effective search space used: 598059455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)