BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4378.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 516 e-148 gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [H... 213 1e-56 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 110 1e-25 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 110 1e-25 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 110 2e-25 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 516 bits (1329), Expect = e-148, Method: Compositional matrix adjust. Identities = 249/263 (94%), Positives = 253/263 (96%) Query: 1 MTAATTPVFEHALYRSLAGKRVVITGGGSGIGAALVEAFVAQGAQVYFLDIATAPSEALV 60 MTAATTP FEHALYRSLAGKRVVITGGGSGIGAALVEAFV QGAQV FLDIA PS+ALV Sbjct: 1 MTAATTPAFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALV 60 Query: 61 EALKAAPVAPRFFPCNLMNLEALRATFTEIETIAGGVDILINNAANDDRHKTADVTPAYW 120 +LK AP+APRFFPCNLMNLEALRATFTEIET+ GGVDILINNAANDDRHKTADVTPAYW Sbjct: 61 ASLKDAPIAPRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYW 120 Query: 121 DERLAVNLRHQFFCAQAVLPGMRERKSGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH 180 DERLAVNLRHQFFCAQAVLPGMRERK GVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH Sbjct: 121 DERLAVNLRHQFFCAQAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH 180 Query: 181 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPVRVDPHDVAALALF 240 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLP RVDPHDVAALALF Sbjct: 181 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALF 240 Query: 241 LSSDSGAKCTGREYYVDAGWLGA 263 LSSDSGAKCTGREYYVDAGWLGA Sbjct: 241 LSSDSGAKCTGREYYVDAGWLGA 263 >gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 213 bits (542), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 122/251 (48%), Positives = 159/251 (63%), Gaps = 1/251 (0%) Query: 12 ALYRSLAGKRVVITGGGSGIGAALVEAFVAQGAQVYFLDIATAPSEALVEALKAA-PVAP 70 A + SL GKRV ITGGG+GIGAA+VEAF QGA V F+DIAT SEAL + AA P Sbjct: 11 ASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCNEVAAAGHPKP 70 Query: 71 RFFPCNLMNLEALRATFTEIETIAGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRH 130 F C+L ++ A +AT E++ G D+L+NNAAND RHK +VT YW++R+A+N R Sbjct: 71 LFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYWNDRIAINQRP 130 Query: 131 QFFCAQAVLPGMRERKSGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDG 190 FF Q+V+ GM+ R G I+NF SISWH +Y TAKA G+T G+ARD G Sbjct: 131 SFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKASTLGLTRGLARDLGPHK 190 Query: 191 VRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPVRVDPHDVAALALFLSSDSGAKCT 250 +RVN + PG + T RQ LW E + I QCL + P +A + LFL++D A CT Sbjct: 191 IRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLFLAADDSAMCT 250 Query: 251 GREYYVDAGWL 261 +E+ VDAGW+ Sbjct: 251 AQEFIVDAGWV 261 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 110 bits (275), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 87/254 (34%), Positives = 128/254 (50%), Gaps = 18/254 (7%) Query: 17 LAGKRVVITGGG--SGIGAALVEAFVAQGAQVYFLDIATAPSEALVEALKAAPVAPRFFP 74 L K +ITGG +G+G + AQGA++ LD+ A L A + Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLG---LV 59 Query: 75 CNLMNLEALRATFTEIETIAGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRHQFFC 134 ++ + A A + G +DIL+NNA K ++T +D L V+LR + Sbjct: 60 ADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYM 119 Query: 135 AQAVLPGMRERKSGVILNFGSISWH-----LGLPDLTLYMTAKAGIEGMTHGMARDFGRD 189 +QAVLP M+ ++SG I+ S+S LG P Y AKAG+ G+ MAR+FG Sbjct: 120 SQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPH---YSAAKAGVLGLARAMAREFGPQ 176 Query: 190 GVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPV-RV-DPHDVAALALFLSSDSGA 247 G+RVN I PG I T + + E++A+I AQ +P+ R+ P DV + +FL+SD Sbjct: 177 GIRVNCITPGLISTDINKDIL-SAEKKAEI--AQTIPLGRLGGPDDVGGVCVFLASDLSQ 233 Query: 248 KCTGREYYVDAGWL 261 CTG V+ G L Sbjct: 234 YCTGVTLDVNGGML 247 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 110 bits (274), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 78/259 (30%), Positives = 117/259 (45%) Query: 1 MTAATTPVFEHALYRSLAGKRVVITGGGSGIGAALVEAFVAQGAQVYFLDIATAPSEALV 60 MT TTP+ SL GK +ITG GIG AL GA + A+ Sbjct: 1 MTTKTTPLPAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVA 60 Query: 61 EALKAAPVAPRFFPCNLMNLEALRATFTEIETIAGGVDILINNAANDDRHKTADVTPAYW 120 L+A + + E ++A +IE+ G + IL+NNA R+ + + W Sbjct: 61 AQLRAEGHTIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDW 120 Query: 121 DERLAVNLRHQFFCAQAVLPGMRERKSGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH 180 + ++ NL FF AQAV M R +G I+N S+ LG P + Y K ++ T Sbjct: 121 HDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK 180 Query: 181 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPVRVDPHDVAALALF 240 GMA D+G+ G++VN + PG +T L + + ++ D D+ A+F Sbjct: 181 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVF 240 Query: 241 LSSDSGAKCTGREYYVDAG 259 L+SD+ G YVD G Sbjct: 241 LASDASRFVNGHILYVDGG 259 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 88/256 (34%), Positives = 126/256 (49%), Gaps = 22/256 (8%) Query: 17 LAGKRVVITGGG--SGIGAALVEAFVAQGAQVYFLDIATAPSEALVEALKAAPVAPRFFP 74 L K VITGG +G+G A QGA+V LD+A A A + + + Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLG---LV 59 Query: 75 CNLMNLEALRATFTEIETIAGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRHQFFC 134 ++ + + I G +DIL+NNA KT ++T A +D L V+LR + Sbjct: 60 ADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYM 119 Query: 135 AQAVLPGMRERKSGVILNFGSISWH-----LGLPDLTLYMTAKAGIEGMTHGMARDFGRD 189 +QAVLP MR +++G I+ S+S LG P Y AKAG+ G+ MAR+FG + Sbjct: 120 SQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPH---YSAAKAGVLGLARAMAREFGPE 176 Query: 190 GVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPV----RVDPHDVAALALFLSSDS 245 G+RVN+I PG I T ++ EE K A+ +P+ R D D+ +FL SD Sbjct: 177 GIRVNSITPGLIGTD---IIKGKLTEEKKAEIAETIPLARLGRAD--DIGGACVFLGSDL 231 Query: 246 GAKCTGREYYVDAGWL 261 CTG V+ G L Sbjct: 232 SQYCTGITLDVNGGML 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 886,322 Number of extensions: 38158 Number of successful extensions: 296 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 46 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)