BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4370.p (349 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogen... 676 0.0 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 172 4e-44 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 75 9e-15 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 66 4e-12 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 55 8e-09 >gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 676 bits (1744), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/349 (97%), Positives = 347/349 (99%) Query: 1 MQVHAYGAHAADKPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQVRSEWAGTQYPCVP 60 MQVHAYGAHAADKPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQ+RSEWAGTQYPCVP Sbjct: 1 MQVHAYGAHAADKPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQIRSEWAGTQYPCVP 60 Query: 61 GHEIVGRVAAVGAHVSKFKVGDLVGVGCIVDSCQSCPDCEEGLENYCDNMVGTYNGKTDD 120 GHEIVGRVAAVGAHVSKFKVGD+VGVGCIVDSCQSCPDC+EGLENYCDNMVGTYNGKTDD Sbjct: 61 GHEIVGRVAAVGAHVSKFKVGDVVGVGCIVDSCQSCPDCDEGLENYCDNMVGTYNGKTDD 120 Query: 121 APGWTMGGYSQQIVVHERYVLTIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGV 180 APGWTMGGYSQQIVVHERYVL IRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGV Sbjct: 121 APGWTMGGYSQQIVVHERYVLNIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGV 180 Query: 181 VGIGGLGHMGIKIAHAMGAHVVAFTTSDSKRAAAKALGADEVVVSRNAEEMAAHAKSFDF 240 VGIGGLGHMGIKIAHAMGAHVVAFTTSDSKRA AKALGADEVVVSRNAEEMAAHAKSFDF Sbjct: 181 VGIGGLGHMGIKIAHAMGAHVVAFTTSDSKRADAKALGADEVVVSRNAEEMAAHAKSFDF 240 Query: 241 ILNTVAAPHDLDAFLSLLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGGIPET 300 ILNTVAAPHDLDAFL+LLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGGIPET Sbjct: 241 ILNTVAAPHDLDAFLALLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGGIPET 300 Query: 301 QEMLDFCAQHGIVADIELIRAEEINSAYERMLKGDIKYRFVIDNASLAA 349 QEMLDFCA+HGIVADIELIRA+EIN+AYERMLKGD+KYRFVIDNASLAA Sbjct: 301 QEMLDFCAEHGIVADIELIRADEINTAYERMLKGDVKYRFVIDNASLAA 349 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 172 bits (435), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 115/336 (34%), Positives = 163/336 (48%), Gaps = 16/336 (4%) Query: 13 KPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQVRSEW-AGTQYPCVPGHEIVGRVAAV 71 KPL + + + G+CH+DLH +W P +PGHE G VAAV Sbjct: 15 KPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFIPGHEGTGYVAAV 74 Query: 72 GAHVSKFKVGDLVGVGCIVDSCQSCPDCEEGLENYCDNMVGTYNGKTDDAPGWTMGG-YS 130 GA V K GD VGV + +C C C G E C T G+++ G ++ Sbjct: 75 GAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNT---------GYSVNGSFA 125 Query: 131 QQIVVHERYVLTIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGVVGIGGLGHMG 190 + + ++V + D AP+LCAG+T Y L+ PG+ V + GIGGLGHM Sbjct: 126 EYGLADPKFVGHLPD-NLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGLGHMA 184 Query: 191 IKIAHAMGAHVVAFTTSDSKRAAAKALGADEVVVSRNAEEMAAHAKSFD---FILNTVAA 247 ++ A AMG HVVA + K A AK LGAD V RN +A + L T + Sbjct: 185 VQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGGAHGALVTAVS 244 Query: 248 PHDLDAFLSLLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGGIPETQEMLDFC 307 P ++ L+ GTM LVG P P VFN +LKR ++ GS++G + +E L F Sbjct: 245 PKAMEQAFGFLRARGTMALVGLPPGDISLP-VFNTVLKRITVRGSIVGTRQDLEESLVFA 303 Query: 308 AQHGIVADIELIRAEEINSAYERMLKGDIKYRFVID 343 A+ + A + + IN+ + RM +G I R V+D Sbjct: 304 AEGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLD 339 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 75.1 bits (183), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 87/308 (28%), Positives = 131/308 (42%), Gaps = 27/308 (8%) Query: 10 AADKPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQVRSEWAGTQYPCVPGHEIVGRVA 69 A +PL+ + G +V + + G+CHSDL V + P GHE V Sbjct: 18 AVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDL-SVITGVRPRPVPMALGHEASAEVV 76 Query: 70 AVGAHVSKFKVGDLVGVGCIVDSCQSCPDCEEGLENYCD-----NMVGTYNGKTDDAPGW 124 VG+ V+ + GDLV V V SC C C EG C+ N VG Sbjct: 77 EVGSGVNDLRAGDLV-VLVFVPSCGHCLPCMEGRPALCEPGAATNTVGELLSGQRRLRDK 135 Query: 125 T--------MGGYSQQIVVHERYVLTIRHPEADLAAVAPLL--CAGITTY-SPLRHWGAG 173 T + ++ VV R + I EAD+ V L CA +T + + Sbjct: 136 TQPVHHHLGVSAFADHAVVSRRSCVKI---EADIDPVQAALFGCAVLTGVGAAVNTAEVK 192 Query: 174 PGKKVGVVGIGGLGHMGIKIAHAMGAH-VVAFTTSDSKRAAAKALGADEVVVSRN---AE 229 G V+G+GG+G + A A GA V+A DSK A A++LGA V +R+ E Sbjct: 193 AGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVE 252 Query: 230 EMAAHAK-SFDFILNTVAAPHDLDAFLSLLKREGTMTLVGAPAT-PHPSPNVFNLILKRR 287 ++ A + D+ + ++A + +R G G PA H + +L+ + R Sbjct: 253 KVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEER 312 Query: 288 SLAGSLIG 295 ++ GS IG Sbjct: 313 TVKGSYIG 320 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 66.2 bits (160), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 70/264 (26%), Positives = 113/264 (42%), Gaps = 21/264 (7%) Query: 18 LHITRRATGA---HDVQIDIAYCGICHSDLHQVRSEWAGT---QYPCVPGHEIVGRVAAV 71 L I TGA +++ + GIC SDLH G + P V GHE+ G++A V Sbjct: 13 LRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 Query: 72 GAHVSKFKVGDLVGVGCIVDSCQSCPDCEEGLENYCDNMVGTYNGKTDDAPGWTMGGYSQ 131 G V+ G + + C +C C+EG +N+C +M + G P G + Q Sbjct: 73 GTAVTDIAPGTRIAISP-SRPCGACKYCQEGKQNHCLDM--RFYGSAMRTP-HVQGAFRQ 128 Query: 132 QIVVH--ERYVLTIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGVVGIGGLGHM 189 +I++ + +V+ + + A PL A +R G GK+V V G G +G + Sbjct: 129 EIIIERSQAHVVADHVSDGEAAMAEPLSVA----LHAVRRAGPLVGKRVLVTGCGPIGAL 184 Query: 190 GIKIAHAMGA-HVVAFTTSDSKRAAAKALGADEVV-VSRNAEEMAAHAK---SFDFILNT 244 + A GA VV + AA +GAD + ++ + A+A +FD + Sbjct: 185 VVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLFEA 244 Query: 245 VAAPHDLDAFLSLLKREGTMTLVG 268 L L L+ + + VG Sbjct: 245 SGNERALVGALEALRPQAIIVQVG 268 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 55.1 bits (131), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 54/192 (28%), Positives = 81/192 (42%), Gaps = 18/192 (9%) Query: 27 AHDVQIDIAYCGICHSDLHQVRSEWAGTQYPCVPGHEIVGRVAAVGAHVSKFKVGDLVGV 86 A DV + + IC SDLH R + T+ + GHE +G V G + + K GD V V Sbjct: 25 ADDVILKVTATAICGSDLHLYRGKIPATKAGDILGHEFMGVVVEAGPAIKRLKKGDRVVV 84 Query: 87 GCIVDSCQSCPDCEEGLENYCD----------NMVGTYNGKT----DDAPGWTMGGYSQQ 132 ++ +C C C++ L C+ N G +G G GG ++ Sbjct: 85 PFVI-ACGQCFFCDQHLYAACETTNPDRGALMNAKGIRSGAAMFGYSHLYGGMPGGQAEY 143 Query: 133 IVVHERYVLTIRHPEADLAAVAPLLCAGI--TTYSPLRHWGAGPGKKVGVVGIGGLGHMG 190 + V + V IR A LA L + I T Y +++ G G V + G G +G M Sbjct: 144 VRVPKADVGPIRIDSA-LADEQVLFLSDILPTGYQAVKNAGVREGSSVAIYGAGPVGQMA 202 Query: 191 IKIAHAMGAHVV 202 A +GA + Sbjct: 203 AASARMLGAERI 214 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,076,057 Number of extensions: 43809 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 6 Length of query: 349 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 263 Effective length of database: 1,212,660 Effective search space: 318929580 Effective search space used: 318929580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)