BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.4355.p
         (311 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2...   149   2e-37
gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578...    89   5e-19

>gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 281

 Score =  149 bits (377), Expect = 2e-37,   Method: Compositional matrix adjust.
 Identities = 77/186 (41%), Positives = 116/186 (62%), Gaps = 9/186 (4%)

Query: 94  IGLNYVEHVEESSRTLDTSKDLPKQPVIFSKPPTTVIGQGDAIEHNQAITRQMDWEVELA 153
           IGLNY +H  ES+  +      P +PV+F+K  + V+G  D ++  +  +++ DWEVEL 
Sbjct: 76  IGLNYADHAAESNLPI------PAEPVVFNKWTSAVVGPNDNVKIPRG-SKKTDWEVELG 128

Query: 154 VIIGKQAKGVSQASALDYVFGYSVMNDISARDNR--RAGQWIYSKGQDTYAPFGPVIVTA 211
           VIIGK    + +  A+ +V GY V+ND+S R+ +  R G W   KG DT+ P GP +VT 
Sbjct: 129 VIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGPIGPWLVTR 188

Query: 212 DEIPDPHNLDLSLTVNGVTKQSSNTRHMLFKVDTLIADISAGITLEPGDIIATGTPAGVG 271
           DE+ DP  L + L V+G   Q+ NT  M+F V  +++ +S  ++L+PGD+I+TGTP GVG
Sbjct: 189 DEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVISTGTPPGVG 248

Query: 272 AGRSPQ 277
            G  P+
Sbjct: 249 MGVKPE 254


>gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578
           [Herbaspirillum seropedicae SmR1]
          Length = 227

 Score = 89.0 bits (219), Expect = 5e-19,   Method: Compositional matrix adjust.
 Identities = 61/191 (31%), Positives = 93/191 (48%), Gaps = 12/191 (6%)

Query: 87  PRKNIFGIGLNYVEHVEESSRTLDTSKDLPKQPVIFSKPPTTVIGQGDAIEHNQAITRQM 146
           P + I+ +G NY  H  E     D      + P  F KP   V+     + +  A T   
Sbjct: 22  PVRRIYCVGRNYAAHAREMGHDPDR-----EPPFFFMKPADAVVPTDSVVPYPVA-TSDY 75

Query: 147 DWEVELAVIIGKQAKGVSQASALDYVFGYSVMNDISARD----NRRAGQ-WIYSKGQDTY 201
             E+EL V + K  + +    AL+ VFGY+V  D++ RD     ++ G+ W  +KG D  
Sbjct: 76  HHEIELVVALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAKKLGRPWDMAKGFDQS 135

Query: 202 APFGPVIVTADEIPDPHNLDLSLTVNGVTKQSSNTRHMLFKVDTLIADISAGITLEPGDI 261
           AP  P++  A +   P    + L VNG  +Q  +   +++ V   I+ +S  + L PGD+
Sbjct: 136 APCAPIVPVA-QAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETISYLSGLVELFPGDL 194

Query: 262 IATGTPAGVGA 272
           I TGTP GVGA
Sbjct: 195 IFTGTPEGVGA 205


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,054,180
Number of extensions: 46726
Number of successful extensions: 135
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 130
Number of HSP's successfully gapped: 2
Length of query: 311
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 226
Effective length of database: 1,217,395
Effective search space: 275131270
Effective search space used: 275131270
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 130 (54.7 bits)