BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4332.p (371 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 98 1e-21 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 92 8e-20 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 88 1e-18 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 79 6e-16 gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbas... 78 9e-16 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 104/350 (29%), Positives = 164/350 (46%), Gaps = 44/350 (12%) Query: 42 MPPLPVSTIRAEARAVPENLQVVASLQAVREVQLAPDTSGRVTAIHFESGQTVKQGGLLV 101 MPP VS + + V ++ ++A R Q+ +G V F+ G VK+G +L Sbjct: 34 MPPSEVSVVTIAPQRVAITNELPGRVEAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLF 93 Query: 102 EL----YDAP-EQADRAAAVAKADFAQLQL--RRSRELSPAGAESRELLE-----QRQAD 149 ++ Y A E A A A+A+ Q L +R + L A S++ + Q+QA+ Sbjct: 94 KIDPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAE 153 Query: 150 ADQAV--AAVRQLDARIEQKNIRAPFSGQIGIRHVNPGQYLNAGDA--IATLTQADPLYV 205 AD A AAV+ + + +P SG+IG V G + GDA +A + Q DP+YV Sbjct: 154 ADVAAGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDPVYV 213 Query: 206 NFQLPQQDLSRL----------TVG---ASVQVSVDA-----VPGRVFTGKISSIEPRID 247 N DL +L +VG ASV + ++ +PG++ IS +D Sbjct: 214 NVTQSSADLLKLQQAMAAGQLKSVGDNKASVTLELENGQAYPLPGKLLFSDIS-----VD 268 Query: 248 GETRNVAVQAILPNADRLLKSGMYATARLDLPAVEDGIVLPLTAIQTSASGDSVVLVRGA 307 T +V+++A PN RLL G Y A+++ E +++P A+ A G +V++V Sbjct: 269 ENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRDAGGSTVMVVNAE 328 Query: 308 DAQGIGKAVAVPVVTGQRLNEEVLVTQGVKAGDIVVMAGQNRLPPGAVVK 357 + K PV TG V G+K GD V++ G ++ PGA VK Sbjct: 329 N-----KVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATVK 373 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 91.7 bits (226), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 92/312 (29%), Positives = 149/312 (47%), Gaps = 36/312 (11%) Query: 77 PDTSGRVTAIHFESGQTVKQGGLLVELYDAPEQA---DRAAAVAKAD----FAQLQLRRS 129 P +G + A F G VK G +L ++ A QA AA+AKA+ A+L+ R Sbjct: 71 PQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRY 130 Query: 130 RELSPAGAESRE-------LLEQRQADADQAVAAVRQLDARIEQKNIRAPFSGQIGIRHV 182 +EL A S++ L + +AD A A V+ ++ + AP SG+IG V Sbjct: 131 KELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSV 190 Query: 183 NPGQYLNAGD--AIATLTQADPLYVNFQ------------LPQQDLSRLTVGASVQVSVD 228 PG + A A+ T+ Q DP+YV+ L L + GA V++ ++ Sbjct: 191 TPGALVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSGNGAKVRLVLE 250 Query: 229 AVPGRVFT--GKISSIEPRIDGETRNVAVQAILPNADRLLKSGMYATARLDLPAVEDGIV 286 GR + G + + ++ +T + ++AI PN L GMY A L + G++ Sbjct: 251 N--GRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLL 308 Query: 287 LPLTAIQTSASGDSVVLVRGADAQGIGKAVAVPVVTGQRLNEEVLVTQGVKAGDIVVMAG 346 +P A+ +G V V +AQG+ + A +VT + + ++ LV+ G+K GD +V+ G Sbjct: 309 VPQQAVSRDGAGKPVAFV--VNAQGVVEQRA--LVTDRAIGDQWLVSSGLKEGDQLVVDG 364 Query: 347 QNRLPPGAVVKI 358 Q R GA VK+ Sbjct: 365 QQRASAGAKVKV 376 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 88.2 bits (217), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 96/347 (27%), Positives = 166/347 (47%), Gaps = 32/347 (9%) Query: 41 AMPPLPVSTIRAEARAVPENLQVVASLQAVREVQLAPDTSGRVTAIHFESGQTVKQGGLL 100 A PP+ V+ + R +P+ L V ++ A V + G++ + F+ G+ VK+G LL Sbjct: 45 APPPVSVTLASVQERDLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLL 104 Query: 101 VELYDAPEQADRAAAV-------AKADFAQLQLRRSRELSPAGAESRELLEQRQADADQA 153 ++ QA A A A A+L L+R L A +++ L+ ++A Q Sbjct: 105 AQIDARALQAQLAQAEAQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQL 164 Query: 154 VAAVRQLDARI-------EQKNIRAPFSGQIGIRHVNPGQYLNAGDA--IATLTQADPLY 204 AAV+ +A++ I AP SG++G R V+PG ++A DA + + Q DP+ Sbjct: 165 EAAVKTDEAQVAYAKVQLSYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPIT 224 Query: 205 VNFQLPQQDLSRLTVGAS-----VQVSVDAVPGR--VFTGKISSIEPRIDGETRNVAVQA 257 + F LP++ ++ + +QVS A + + TGK+ ++ +I+ V ++A Sbjct: 225 LVFTLPEEAVAGINRAQRAHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKA 284 Query: 258 ILPNADRLLKSGMYATARLDLPAVEDGIVLPLTAIQTSASGDSVVLVRGADAQGIGKAVA 317 N D L G Y ARL + + + + +P AIQ + G V + D +A Sbjct: 285 RFDNPDHKLWPGQYVNARLQMRSHGNALTVPAAAIQRNQQGTYVYAIDQED-----QARM 339 Query: 318 VPVVTGQRLNEEVLVT---QGVKAGDIVVMAGQNRLPPGAVVKINAP 361 PV +R+ + + V V+AG VV+ GQ +L PG+ + + P Sbjct: 340 QPVKV-ERIQDGIAVLAAGNAVQAGQRVVIDGQYKLKPGSRIVESKP 385 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 79.0 bits (193), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 85/307 (27%), Positives = 140/307 (45%), Gaps = 41/307 (13%) Query: 80 SGRVTAIHFESGQTVKQGGLLVE-----LYDAPEQADRAAAVAKADFA--QLQLRRSREL 132 G++ +H++ GQ VK G LL E L A QA+ A +A A +L L+R + L Sbjct: 67 DGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTL 126 Query: 133 SPAGAESRELLEQRQADADQAVAAVRQLD------------ARIEQKNIR--APFSGQIG 178 +++ + +Q D VA V+Q + AR++ R AP SG++G Sbjct: 127 L-----AQDSIASQQVDTQ--VALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLG 179 Query: 179 IRHVNPGQYLNAGDA--IATLTQADPLYVNFQLPQQD----LSRLTVGASV--QVSVDAV 230 +R + G + D + +TQ P+ F +P+ + L +L G + Q Sbjct: 180 LRQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPAQAWDREQ 239 Query: 231 PGRVFTGKISSIEPRIDGETRNVAVQAILPNADRLLKSGMYATARLDLPAVEDGIVLPLT 290 ++ G + +I+ +D T V ++A PN D L + RL L + V+P Sbjct: 240 KNKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTA 299 Query: 291 AIQTSASGDSVVLVRGADAQGIGKAVAVPVVTGQRLNEEVLVTQGVKAGDIVVMAGQNRL 350 AIQ + G V +V+ + PV TG ++ ++T GV AG+ VV+ G +RL Sbjct: 300 AIQRGSKGLFVYVVKDDSS-----VTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRL 354 Query: 351 PPGAVVK 357 GA V+ Sbjct: 355 REGAKVE 361 >gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 78.2 bits (191), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 104/386 (26%), Positives = 159/386 (41%), Gaps = 48/386 (12%) Query: 15 SVVGLVVVFGALYAVRSLRSGGAEHYAMPPLPVSTIRAEARAVPENLQVVASLQAVREVQ 74 S V LV+V G Y + S + P+ + T+ E + + + ++ A V Sbjct: 26 SSVALVIVLGGGYWLYSRQQPPPPAPKPIPVKLETVTVERADLEQVVNATGNVVAQDYVD 85 Query: 75 LAPDTSGRVTAIHFESGQTVKQGGLLVELYDAPE--------------QADRAAAVAKAD 120 + +G+++ + G+TVK G LL + A + +A+ A A+ D Sbjct: 86 VGSKVAGQISDVDVVVGETVKAGKLLATVAPAVQSSRIENNRATLARLKAELAGQNAQLD 145 Query: 121 FAQLQLRRSRELSPAGAESRELLEQRQ--------------ADADQAVAAVRQLDARIEQ 166 FAQLQ +R +L A E E + A Q AA+R+ +A +Q Sbjct: 146 FAQLQFQRQTQLKAENATREESYESSRMNMYAAAARVDATNAQIQQTEAAIREDEAVQKQ 205 Query: 167 KNIRAPFSGQIGIRHVNPGQYLNAGDAIATLTQADPLYVNFQLP--QQDLSRLTVGASVQ 224 I AP SG I + PGQ + A + A + AD + Q+P ++D++RL G + Sbjct: 206 TRIEAPVSGTIVTLNARPGQMV-AANQEALMRIADLSRMTVQVPVAEEDVTRLQKGMTAY 264 Query: 225 VSVDAVPGRVFTGKISSIE--PRID----GETRNVAVQAILPNADRLLKSGMYATARLDL 278 + PG+ ++GK+ I P D G+ V + N R L SGM A L Sbjct: 265 FTTPGYPGKRWSGKLRQIMLLPTDDSGRQGKKAFYTVFFDVANPSRELMSGMSADVYFVL 324 Query: 279 PAVEDGIVLPLTAI-QTSASGDSVVLVRGADAQGIGKAVAVPVVTGQRLNEEVLVTQGVK 337 E +P T + + G V V AD GK + G R NE V G+K Sbjct: 325 ARAEHVPTIPRTLVTKPGMDGTQTVKVVLAD----GKLETRKIKIGIRDNERAQVLSGLK 380 Query: 338 AGDIVVMAGQNRLPPGAVVKINAPAA 363 G+ V+ LP APAA Sbjct: 381 EGEQVL------LPANPAASTVAPAA 400 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,021,294 Number of extensions: 40059 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 221 Number of HSP's successfully gapped: 12 Length of query: 371 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 284 Effective length of database: 1,207,925 Effective search space: 343050700 Effective search space used: 343050700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)