BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4326.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 459 e-130 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 411 e-116 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 355 3e-99 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 343 1e-95 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 323 1e-89 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 459 bits (1180), Expect = e-130, Method: Compositional matrix adjust. Identities = 231/468 (49%), Positives = 318/468 (67%), Gaps = 3/468 (0%) Query: 14 YIGGQWREASDGARFDVLDPATEELLTEVASGSIADALDAVGAAYQAGPAWAATSPRQRS 73 ++ GQW +A G R V +PA ELL V A+ A+ AA A PAW + ++RS Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 74 DILRKAFDLLIERKEAFARLIVLESGKALAEARGEVAYAAEFLRWYSEEAVRIQGEVSVA 133 ILR+ ++L++ + A ++ E GK LAEARGE+ YAA F+ W++EE R G+ + Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 134 PGGANRILVVRQPIGVAVFVTPWNFPAAMATRKIGPALAAGCTVVLKPASETPLTALAMA 193 P +NRI+V+++ IGV +TPWNFPAAM TRK GPALAAGC +VLKPA TP +ALA+A Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALA 194 Query: 194 EVFEEAGVPAGVINVVPSRKSGEIVKAMLEDPRVRKLSFTGSTEIGRVLLGQAAQNVVKC 253 + E AG+PAGV +VV G I M +P VRK+SFTGST +G++L+ Q+A ++ K Sbjct: 195 VLAERAGIPAGVFSVVTGTPKG-IGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKL 253 Query: 254 SMELGGNAPFIVLADADLEVAVASAMVAKLRNGGESCTAANRFYVERPVAEEFSRRFAQA 313 S+ELGGNAPFIV DADL+ AV A+ +K RN G++C ANR YV+ V + F+ + +A Sbjct: 254 SLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEA 313 Query: 314 MAAVKIGSGLEEGIQLGPLVNSSTLQKVHQLVTEAVEQGAKVLTGGKIREGKGY-FYEPT 372 + K+G G EEG+ GPL+N +QKV Q V +AV +GA+VL GGK R G+ F+EPT Sbjct: 314 VKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK-RHALGHSFFEPT 372 Query: 373 LLTDVRADATILHREIFGPVAPICIFDSIDEVIRLANDTEFGLVSFVHSKDLGKALQVAE 432 +L DV + E FGP+AP+ F + +EV+ LANDTEFGL S+ +S+D+G+ +VAE Sbjct: 373 VLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAE 432 Query: 433 RIEAGMVGINRGVVSDPAAPFGGWKQSGVGREGGHDGLLEYLESKYIC 480 +E+GMVGIN G++S+ APFGG KQSG+GREG H G+ +YL KY+C Sbjct: 433 GLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 411 bits (1057), Expect = e-116, Method: Compositional matrix adjust. Identities = 225/472 (47%), Positives = 312/472 (66%), Gaps = 6/472 (1%) Query: 15 IGGQWREASDGARFDVLDPATEELLTEVASGSIADALDAVGAAYQAGPAWAATSPRQRSD 74 IG WR A+DG + DV DPAT ++ V G ADA AV AA A AW AT +QR+ Sbjct: 18 IGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRATPAKQRAG 77 Query: 75 ILRKAFDLLIERKEAFARLIVLESGKALAEARGEVAYAAEFLRWYSEEAVRIQGEVSVAP 134 I+++ DLL+ ++ RLI E GK LAEA+GEVAYAA ++ W+ EEA R G++ AP Sbjct: 78 IIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIPAP 137 Query: 135 GGANRILVVRQPIGVAVFVTPWNFPAAMATRKIGPALAAGCTVVLKPASETPLTALAMAE 194 R++ +++P+GV +TPWNFPAAM RKI PALAAGCTVV KPA +TPLT+LA+ Sbjct: 138 VTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLALVR 197 Query: 195 VFEEAGVPAGVINVVPS--RKSGEIVKAMLEDPRVRKLSFTGSTEIGRVLLGQAAQNVVK 252 + +EAGVP GVIN+V + ++ E+V L D RVRK+SFTGST +G+ L +A + K Sbjct: 198 LAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTLKK 257 Query: 253 CSMELGGNAPFIVLADADLEVAVASAMVAKLRNGGESCTAANRFYVERPVAEEFSRRFAQ 312 S+ELGGNAPFIV DAD++ A+ M AK RNGG++C + NR YV+ V + F + Sbjct: 258 LSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVDKLGA 317 Query: 313 AMAAVKIGSGLEEGIQLGPLVNSSTLQKVHQLVTEAVEQGAKVLTGGKIREGKGY----F 368 +AA+K+G + Q+GP++N+ + K+ Q V +A+ +GA+V+TGGK +G G+ + Sbjct: 318 RVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFGSDNY 377 Query: 369 YEPTLLTDVRADATILHREIFGPVAPICIFDSIDEVIRLANDTEFGLVSFVHSKDLGKAL 428 Y PT+L DV E FGPVAPI F + DEVI AN T FGL ++ +S D+ + Sbjct: 378 YAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTDVRRIH 437 Query: 429 QVAERIEAGMVGINRGVVSDPAAPFGGWKQSGVGREGGHDGLLEYLESKYIC 480 +V + +E+G+VG+N G ++ AAPFGG K+SG GREG GL +YL +KY+C Sbjct: 438 RVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYVC 489 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 355 bits (912), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 189/470 (40%), Positives = 286/470 (60%), Gaps = 5/470 (1%) Query: 13 LYIGGQWREASDGARFDVLDPATEELLTEVASGSIADALDAVGAAYQAGPAWAATSPRQR 72 L+I G+W ++ G DV++PATEE++ ++A AD A+ AA + W S +R Sbjct: 7 LFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFER 66 Query: 73 SDILRKAFDLLIERKEAFARLIVLESGKALAEARGEVAYAAEFLRWYSEEAVRIQGEVSV 132 S I+R+A DLL ER E ARL+ +E GK LAEA+ E AA+ + W++EEA R G + Sbjct: 67 SKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVP 126 Query: 133 A--PGGANRILVVRQPIGVAVFVTPWNFPAAMATRKIGPALAAGCTVVLKPASETPLTAL 190 A PG +V+++P+G TPWNFP RK+ ALAAGC++++K ETP + Sbjct: 127 ARVPGAYQ--MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPA 184 Query: 191 AMAEVFEEAGVPAGVINVVPSRKSGEIVKAMLEDPRVRKLSFTGSTEIGRVLLGQAAQNV 250 + + +AGVPAGVIN+V + EI + ++ P ++K+SFTGST +G+ L A Q++ Sbjct: 185 ELIRAYADAGVPAGVINLVYGVPA-EISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHM 243 Query: 251 VKCSMELGGNAPFIVLADADLEVAVASAMVAKLRNGGESCTAANRFYVERPVAEEFSRRF 310 + +MELGG+AP +V DAD++VA AK RN G+ C + RF V++ V F +F Sbjct: 244 KRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 Query: 311 AQAMAAVKIGSGLEEGIQLGPLVNSSTLQKVHQLVTEAVEQGAKVLTGGKIREGKGYFYE 370 +K+G+GLE GI +GP+ N + + L+ +AV QGA++ TGGK KGYF++ Sbjct: 304 VTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQ 363 Query: 371 PTLLTDVRADATILHREIFGPVAPICIFDSIDEVIRLANDTEFGLVSFVHSKDLGKALQV 430 PT+LT++ A ++ E FGP+A I F+++DEVI AN +GL ++ +K + Sbjct: 364 PTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHAL 423 Query: 431 AERIEAGMVGINRGVVSDPAAPFGGWKQSGVGREGGHDGLLEYLESKYIC 480 A R+E+GM+ IN ++ P PFGG K SG G EGG + + Y+ +K + Sbjct: 424 AARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 343 bits (881), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 200/475 (42%), Positives = 277/475 (58%), Gaps = 11/475 (2%) Query: 13 LYIGGQWREASDGARFDVLDPATEELLTEVASGSIADALDAVGAAYQAGPAWAATSPRQR 72 YI G+W+ R V+ PATEE + E+A G+ AD AV AA +A PAWAATSP +R Sbjct: 18 FYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLER 77 Query: 73 SDILRKAFDLLIERKEAFARLIVLESGKALAEAR-GEVAYAAEFLRWYSEEAVRIQGEVS 131 + +L + LL+ER E FA+ + LE G ++ AR V AAE LR + + Sbjct: 78 AALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVARDN---LADYPF 134 Query: 132 VAPGGANRILVVRQPIGVAVFVTPWNFPAAMATRKIGPALAAGCTVVLKPASETPLTALA 191 + P G I VR+PIGV +TPWN+P T K+GPALAAGCTVVLKP+ +PL+AL Sbjct: 135 IRPRGTTAI--VREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALL 192 Query: 192 MAEVFEEAGVPAGVINVVPSRKSGEIVKAMLEDPRVRKLSFTGSTEIGRVLLGQAAQNVV 251 AE+ EAGVPAGV N+V + E+ A+ P+V +S TGST G ++ AA V Sbjct: 193 FAEIVHEAGVPAGVFNLV-NGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVK 251 Query: 252 KCSMELGGNAPFIVLADADLEVAVASAMVAKLRNGGESCTAANRFYVERPVAEEFSRRFA 311 + + ELGG +P +VL DADLE A+ + A LRN G+SC+A R V R Sbjct: 252 RVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELAL 311 Query: 312 QAMAAVKIGSGLEEGIQLGPLVNSSTLQKVHQLVTEAVEQGAKVLTGGKIRE---GKGYF 368 +A +K+G + GPL N + +V Q++ ++ GA++L GG R +GY+ Sbjct: 312 ATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYY 371 Query: 369 YEPTLLTDVRADATILHREIFGPVAPICIFDSIDEVIRLANDTEFGLVSFVHSKDLGKAL 428 PT+ ++V D I EIFGPV I +D+++E I +ANDT +GL + V +D + Sbjct: 372 ARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVR 431 Query: 429 QVAERIEAGMVGINRGVVSDPAAPFGGWKQSGVGREGGHDGLLEYLESKYICASW 483 VA RI++G V +N DP APFGG+KQSG GRE G +G+ EY+E K I + Sbjct: 432 AVAARIQSGQVHLNYP-AWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAILGYY 485 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 323 bits (829), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 199/469 (42%), Positives = 291/469 (62%), Gaps = 3/469 (0%) Query: 13 LYIGGQWREASDGARF-DVLDPATEELLTEVASGSIADALDAVGAAYQAGPAWAATSPRQ 71 LYI G++ +G R+ +V DPAT ++L + AD +A+ AA++A W SP + Sbjct: 7 LYIDGRF-HGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 Query: 72 RSDILRKAFDLLIERKEAFARLIVLESGKALAEARGEVAYAAEFLRWYSEEAVRIQGEVS 131 RSDILR+A L ER +A A I +++GK LA+A E+ AAE L W++EE R+ G V Sbjct: 66 RSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVV 125 Query: 132 VAPGGANRILVVRQPIGVAVFVTPWNFPAAMATRKIGPALAAGCTVVLKPASETPLTALA 191 A R LVVR+P+GV ++PWNFP A +K+ A+AAGCTVVLK SE+P + Sbjct: 126 GARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVL 185 Query: 192 MAEVFEEAGVPAGVINVVPSRKSGEIVKAMLEDPRVRKLSFTGSTEIGRVLLGQAAQNVV 251 MA++F +AGVPAGV N+V S S EI + ++E P VR +SFTGS +G L QAA ++ Sbjct: 186 MAQLFHDAGVPAGVFNIV-SGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMK 244 Query: 252 KCSMELGGNAPFIVLADADLEVAVASAMVAKLRNGGESCTAANRFYVERPVAEEFSRRFA 311 + SMELGG+AP +V DA++E A K RN G+ C + +RFYV++ + F FA Sbjct: 245 RMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFA 304 Query: 312 QAMAAVKIGSGLEEGIQLGPLVNSSTLQKVHQLVTEAVEQGAKVLTGGKIREGKGYFYEP 371 +A+ +K+G G E G+Q+GPL++ + V L+ +A+ QG++++TGG +G+FY P Sbjct: 305 EALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGHFYAP 364 Query: 372 TLLTDVRADATILHREIFGPVAPICIFDSIDEVIRLANDTEFGLVSFVHSKDLGKALQVA 431 TL+T V A ++ E FGP+A + F +E I +AN GL S+V ++ L A +V+ Sbjct: 365 TLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTAHRVS 424 Query: 432 ERIEAGMVGINRGVVSDPAAPFGGWKQSGVGREGGHDGLLEYLESKYIC 480 ++AGMV IN ++ P PFGG +SG G EGG +G +L +K I Sbjct: 425 RDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMIS 473 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,461,516 Number of extensions: 61140 Number of successful extensions: 274 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 21 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)