BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4319.p (532 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 847 0.0 gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 114 2e-26 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 72 2e-13 gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspi... 66 6e-12 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 847 bits (2189), Expect = 0.0, Method: Compositional matrix adjust. Identities = 430/524 (82%), Positives = 460/524 (87%) Query: 9 ITVRDAVLDLMRRLDMTSVFANPGSTELPMFRDFPADFRYVLGLQEAVVVGMADGYAQAS 68 ITVR AVLDLMRRL MTSVFANPGSTELPMFRDFP DFRYVLGLQEAVVVGMADG+AQAS Sbjct: 9 ITVRHAVLDLMRRLGMTSVFANPGSTELPMFRDFPEDFRYVLGLQEAVVVGMADGFAQAS 68 Query: 69 GKAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLAASHATELPKP 128 G AALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFL A+ ATEL KP Sbjct: 69 GNAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKP 128 Query: 129 YVKWSIEPARAADVPRALARAYHIAMQEPRGPVFVSIPVDDWDQPGELLEATEVTTRSRP 188 Y+KWSIEPARAADVP ALARAYH+AMQEPRGPVFVSIPVDDWDQP EL+ T+VT+R RP Sbjct: 129 YLKWSIEPARAADVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPTQVTSRCRP 188 Query: 189 EPDAIAALATALHRSERPAIVVGSELDRAGAWDEVLRLAEVSNARVFVAPMSSRCSFPED 248 EP AIA LA AL +S+RPAIVVG+ELDRAGAWD + LAE ARVFVAPMSSRCSFPED Sbjct: 189 EPAAIARLAAALEQSQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMSSRCSFPED 248 Query: 249 HHLFAGFLPAMREKIVQLLNGHDLIFVAGAPAFTYHVEGSGPHLPSGAQLYQLIDDPVTA 308 H LFAGFLPAMREKIVQ L GHDL+F GAPAFTYHVEGSGPH+P GA L+QLIDDPVTA Sbjct: 249 HPLFAGFLPAMREKIVQALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQLIDDPVTA 308 Query: 309 SWTPSGTAIVGNIQLGLQDLLAQLLKHPPAARPLPPARAARPRAEPETPMSAAFAMQTLA 368 SWTP+G AIVGNIQLGL DLLAQ+ + PP R PPAR AR R E P+S A+A+QTLA Sbjct: 309 SWTPTGQAIVGNIQLGLDDLLAQIRRQPPTQRAQPPARPARARVEASAPLSMAYALQTLA 368 Query: 369 EVRAAEDIVVEEAPGSRPTMQAYLPFTRPGTFYTMASGGLGYGMPAAVGVALARPQQRVI 428 +VR A DIVVEEAPGSRP MQA+LPFTRPG+FYTMASGGLGYGMPAAVGVALARP RVI Sbjct: 369 DVREAGDIVVEEAPGSRPVMQAHLPFTRPGSFYTMASGGLGYGMPAAVGVALARPHSRVI 428 Query: 429 GLIGDGSAMYSPQAIYSAVQLRLPLTFVIINNRRYAALQDFAPVFGFGAGDVVQGTDLPG 488 GLIGDGSAMYSPQAIYSAVQL LP+TFVI+NN RYAALQDFAPVFGFG D V GT+LPG Sbjct: 429 GLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPVFGFGPEDPVHGTELPG 488 Query: 489 LDFVALAQSMGCQGVRVSEPGELAAALQTAISSNEPRLVEVVVR 532 LDFVALA SMGC GVRVS+ LA AL+ A+ SNE RLVE+VVR Sbjct: 489 LDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLVEIVVR 532 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 114 bits (285), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 145/563 (25%), Positives = 235/563 (41%), Gaps = 59/563 (10%) Query: 1 MQAPQLPGITVRDAVLDLMRRLDMTSVFANPGSTELPMFRD-FPAD-FRYVLGLQEAVVV 58 M A QL G D ++ + + VF PG L ++ F D F+++L E V Sbjct: 1 MSAEQLTGA---DILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAV 57 Query: 59 GMADGYAQASGKAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLA 118 AD Y+++S K + + S GV NA+ + TA+ + P+++ +GQ + + D F Sbjct: 58 HAADAYSRSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQE 117 Query: 119 ASHATELPKPYVKWSIEPARAADVPRALARAYHIAMQEPRGPVFVSIPVDDWDQPGELLE 178 + +P VK + D+ + +A++IA GPV V IP D Sbjct: 118 CD-TVGITRPCVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAY 176 Query: 179 ATEVTTRSRPEPDA-----IAALATALHRSERPAIVVGSELDRAGAWDEVLRLAEVSNAR 233 V RS D I L +ERP I G + A A E+ +L + Sbjct: 177 PKAVEMRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVD----- 231 Query: 234 VFVAPMSSRCSFPEDHHLFA-GFLPAMREKIVQL--LNG---------HDLIFVAGAPAF 281 R +P + L G + +K V + ++G H + +A F Sbjct: 232 --------RLGYPCTNTLMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARF 283 Query: 282 TYHVEGSGPHLPSGAQLYQLID-DPVTASW-TPSGTAIVGNIQLGLQDLLAQL----LKH 335 V G+ H S A+ ID DP + S IVGN++ LQ+LL+QL + Sbjct: 284 DDRVIGNPKHFASHARKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQ 343 Query: 336 PPAARP--LPPARAARPRAEPETPMSAAF-----AMQTLAEVRAAEDIVVEEAPGSRPTM 388 AA R R + S F +Q + EV + + + + Sbjct: 344 NGAALDAWWKQINEWRKRDCLKFATSEEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWA 403 Query: 389 QAYLPFTRPGTFYTMASGGLG---YGMPAAVGVALARPQQRVIGLIGDGSAMYSPQAIYS 445 Y F +P + + SGGLG G+P A+GV +A P V + G+ S Q + + Sbjct: 404 AQYYGFDKPRRW--INSGGLGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELAT 461 Query: 446 AVQLRLPLTFVIINNRRYAALQDFAPVFGFGAGDVVQGTD-LPGLDFVALAQSMGCQGVR 504 Q L +++NNR ++ + + +G+ D LP DF LA+S G G++ Sbjct: 462 CKQYHLTPKIILLNNRFLGMVRQWQQI-DYGSRYSESYMDSLP--DFNKLAESYGHVGMK 518 Query: 505 VSEPGELAAALQTAISSNEPRLV 527 + +PG++ AL+ A + + RLV Sbjct: 519 IEKPGDVDGALREAFAMKD-RLV 540 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 71.6 bits (174), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 51/164 (31%), Positives = 79/164 (48%), Gaps = 7/164 (4%) Query: 373 AEDIVVEEAPGSRPTMQAYLPFTRPGTFYT-MASGGLGYGMPAAVGVALARPQQRVIGLI 431 ++DIVV A + RPG ++ +GY + +GV LA+PQ+ VI ++ Sbjct: 418 SQDIVVCAAGTLPAELHKLWRAGRPGAYHVEYGYSCMGYEVAGGLGVKLAQPQREVIVIV 477 Query: 432 GDGSAMYSPQAIYSAVQLRLPLTFVIINNRRYAALQDF------APVFGFGAGDVVQGTD 485 GDGS + + ++V L L V+++NR Y + AP A + G Sbjct: 478 GDGSYLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAGPG 537 Query: 486 LPGLDFVALAQSMGCQGVRVSEPGELAAALQTAISSNEPRLVEV 529 P +DF A A+S+G G V EL AALQ A +++ LV + Sbjct: 538 APAIDFAAHARSLGALGENVKTITELEAALQRARAADRSYLVCI 581 >gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 66.2 bits (160), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 48/141 (34%), Positives = 71/141 (50%), Gaps = 7/141 (4%) Query: 396 RPGTFYTMASGGLGYGMPAAVGVALARPQQRVIGLIGDGSAMYSPQAIYSAVQLRLPLTF 455 RP T T G +G G+P A+G A+A P + V+GL+ DGS Y+ Q ++S + Sbjct: 412 RPHTVLTNTGGAIGQGLPVALGAAVACPDRTVVGLLSDGSTQYTIQTLWSLAHENARVVV 471 Query: 456 VIINNRRYAAL-----QDFAPVFGFGAGDVVQGTDLPGLDFVALAQSMGCQGVRVSEPGE 510 +I N +YA L +D AP+ A + D P +D+V LAQS G R + Sbjct: 472 LIAANHQYAILRNELRRDGAPLGERAA--QMTALDHPRIDWVGLAQSYGVPASRAETAQQ 529 Query: 511 LAAALQTAISSNEPRLVEVVV 531 L LQ A + P L+E+ + Sbjct: 530 LQQQLQAAFAGTGPALIEMAL 550 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,745,536 Number of extensions: 79638 Number of successful extensions: 270 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 266 Number of HSP's successfully gapped: 5 Length of query: 532 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 442 Effective length of database: 1,193,720 Effective search space: 527624240 Effective search space used: 527624240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)