BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4318.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 749 0.0 gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 274 9e-75 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 271 1e-73 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 270 2e-73 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 235 8e-63 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 749 bits (1933), Expect = 0.0, Method: Compositional matrix adjust. Identities = 410/483 (84%), Positives = 447/483 (92%) Query: 1 MKEVDMLINGQRRSATGGACFERRNPLDESVASRAPAATVADAQSAVRAAAEAFPAWAAM 60 M ++DMLING+RR ATG A FERRNPLD SVA+RAPAATVADA++AV AAA AFPAWAA+ Sbjct: 1 MNDIDMLINGERRGATGAAYFERRNPLDHSVAARAPAATVADARAAVAAAAAAFPAWAAI 60 Query: 61 GPGERRSLLTRAADALQARADAFAEAMASETGASHHWAAFNVHLAADGLREAAAMTTQIA 120 GPG+RR+LLT AADALQARAD FA+AMA+ETGASHHWA FNV LAADGLREAAAMTTQIA Sbjct: 61 GPGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIA 120 Query: 121 GQLIPSDVPGNLAMGVRQAAGVVLGIAPWNAPVILGVRAVALPLACGNTVIFKGSEICPA 180 G++IPSDVPGNLAMGVRQ AGVVLGIAPWNAPVILGVRA+ALPLACGNTV+FKGSEICPA Sbjct: 121 GEVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPA 180 Query: 181 THGLIIDAFQDAGLPPGVVNFITNAPEDAGKIVEAIIADPAVRRVNFTGSTRVGRIIAGQ 240 THGLII A Q+AGLPPGVVNF+TNAPEDA IVEAIIA+PAVRRVNFTGSTRVGRIIA Q Sbjct: 181 THGLIIAALQEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQ 240 Query: 241 CAKYLKPSVLELGGKAPFIVLHDADIEAAVQAAAFGAFANSGQICMSTERIIVDERVADD 300 CA++LKP+VLELGGKAPFIVLHDAD++AAV+AAAFGAFANSGQICMSTERIIVD+ VAD+ Sbjct: 241 CARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADE 300 Query: 301 FVARLVAKARSLPLGDPRQGPVVLGSVVDLATVQRCNALIDDALAKGARLVCGGKAESTL 360 FV RL AKA SLPLGDPRQGPVVLGSVVD+ATV+RCNALIDDALAKGA LVCGG+A TL Sbjct: 301 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTL 360 Query: 361 MPATLLDHVTAQMDIYHEESFGPVKPIVRVKDDDAAIACANDNEYGLSAALFSRDIARAL 420 MPATLLDHV+A M IY EESFGPVKPIVRV D+AAIACANDNEYGLS+ALFSRD+ARAL Sbjct: 361 MPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARAL 420 Query: 421 NLVRRIESGICHINGATVHDEAHMPFGGVKSSGFGHFGGTAGVAEFTDLRWITVQTSQRQ 480 L RRIESGICHING TVHDEA MPFGGVKSSGFGHFGG AG+AEFT+LRW+TVQT+ R Sbjct: 421 ALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFGHFGGKAGIAEFTELRWVTVQTTPRH 480 Query: 481 YPF 483 YPF Sbjct: 481 YPF 483 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 274 bits (701), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 164/426 (38%), Positives = 232/426 (54%), Gaps = 7/426 (1%) Query: 60 MGPGERRSLLTRAADALQARADAFAEAMASETGASHHWAAFNVHLAADGLREAAAMTTQI 119 M P ER + RAA D A +A ETG V A AA M Q Sbjct: 67 MPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQA 126 Query: 120 AGQLIPSDVPGNLAMGVRQAAGVVLGIAPWNAPVILGVRAVALPLACGNTVIFKGSEICP 179 GQL+PS PG L++ R GV+ I+P+N P+IL +R VA LA GN V+ K P Sbjct: 127 QGQLLPS-TPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTP 185 Query: 180 ATHGLII-DAFQDAGLPPGVVNFITNAPEDAGKIVEAIIADPAVRRVNFTGSTRVGRIIA 238 + G ++ F+ AGLP G+++ + E EA++ P V + FTGS VGR I Sbjct: 186 VSGGFMLARIFEQAGLPAGLLHVLPGGAETG----EALVTAPGVPMIAFTGSPAVGRRIG 241 Query: 239 GQCAKYLKPSVLELGGKAPFIVLHDADIEAAVQAAAFGAFANSGQICMSTERIIVDERVA 298 ++LK LELGG ++L DAD++ A AA+GA+ + GQICM+ RI+V + Sbjct: 242 ELAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLE 301 Query: 299 DDFVARLVAKARSLPLGDPRQGPVVLGSVVDLATVQRCNALIDDALAKGARLVCGGKAES 358 + + RL KAR LP+GD G V LG ++D +QR +AL+ +++A GA+LV GG+ E Sbjct: 302 EGLLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQ 361 Query: 359 TLMPATLLDHVTAQMDIYHEESFGPVKPIVRVKDDDAAIACANDNEYGLSAALFSRDIAR 418 T+L V M ++ EE FGPV ++R +D+ AIA AN + GL+A + S + R Sbjct: 362 LFYQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGR 421 Query: 419 ALNLVRRIESGICHINGATVHDEAHMPFGGVKSSGFG-HFGGTAGVAEFTDLRWITVQTS 477 A+ + R+ G+ H+N TV+DE PFGG +G G GG A E+T RW T++ Sbjct: 422 AMAVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQQ 481 Query: 478 QRQYPF 483 YPF Sbjct: 482 AMPYPF 487 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 271 bits (692), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 160/471 (33%), Positives = 245/471 (52%), Gaps = 7/471 (1%) Query: 7 LINGQRRSATGGACFERRNPLDESVASRAPAATVADAQSAVRAAAEAFPAWAAMGPGERR 66 +NGQ A G NP + + P A+ + A+ AA A+PAW ER Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 67 SLLTRAADALQARADAFAEAMASETGASHHWAAFNVHLAADGLREAAAMTTQIAGQLIPS 126 ++L R + + A D A M +E G A + AA + A + G IPS Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 127 DVPGNLAMGVRQAAGVVLGIAPWNAPVILGVRAVALPLACGNTVIFKGSEICPATHGLII 186 P N + +++A GV I PWN P + R LA G ++ K +E P + + Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALA 194 Query: 187 DAFQDAGLPPGVVNFITNAPEDAGKIVEAIIADPAVRRVNFTGSTRVGRIIAGQCAKYLK 246 + AG+P GV + +T P+ G + ++P VR+++FTGST VG+++ Q A +K Sbjct: 195 VLAERAGIPAGVFSVVTGTPKGIGG---EMTSNPIVRKISFTGSTGVGKLLMQQSASSIK 251 Query: 247 PSVLELGGKAPFIVLHDADIEAAVQAAAFGAFANSGQICMSTERIIVDERVADDFVARLV 306 LELGG APFIV DAD++AAV+ A + N+GQ C+ RI V + V D F A+LV Sbjct: 252 KLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLV 311 Query: 307 AKARSLPLGDPRQGPVVLGSVVDLATVQRCNALIDDALAKGARLVCGGKAES---TLMPA 363 + +G + V G +++ VQ+ + DA+AKGAR++ GGK + + Sbjct: 312 EAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEP 371 Query: 364 TLLDHVTAQMDIYHEESFGPVKPIVRVKDDDAAIACANDNEYGLSAALFSRDIARALNLV 423 T+L VT M + EE+FGP+ P+ R K D+ +A AND E+GL++ +SRDI R + Sbjct: 372 TVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVA 431 Query: 424 RRIESGICHINGATVHDEAHMPFGGVKSSGFGHFGGTAGVAEFTDLRWITV 474 +ESG+ IN + +E PFGGVK SG G G G+ ++ ++++ + Sbjct: 432 EGLESGMVGINTGLISNEV-APFGGVKQSGLGREGSHYGIDDYLVVKYLCM 481 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 270 bits (690), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 162/477 (33%), Positives = 253/477 (53%), Gaps = 11/477 (2%) Query: 2 KEVDMLINGQRRSATGGACFERRNPLDESVASRAPAATVADAQSAVRAAAEAFPAWAAMG 61 K+V + ING+ ++ G + NP E V + A AD A+ AA++ F W M Sbjct: 3 KDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMS 62 Query: 62 PGERRSLLTRAADALQARADAFAEAMASETGASHHWAAFNVHLAADGLREAAAMTTQIAG 121 ER ++ RAAD L+ RA+ A M E G A AAD + A + G Sbjct: 63 AFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYG 122 Query: 122 QLIPSDVPGNLAMGVRQAAGVVLGIAPWNAPVILGVRAVALPLACGNTVIFKGSEICPAT 181 +L+P+ VPG M +++ G V PWN P+ VR ++ LA G ++I K E PA+ Sbjct: 123 RLVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPAS 182 Query: 182 HGLIIDAFQDAGLPPGVVNFITNAPEDAGKIVEAIIADPAVRRVNFTGSTRVGRIIAGQC 241 +I A+ DAG+P GV+N + P +I E +I P +++++FTGST VG+ +A Sbjct: 183 PAELIRAYADAGVPAGVINLVYGVP---AEISEYLIPHPVIKKISFTGSTPVGKQLAALA 239 Query: 242 AKYLKPSVLELGGKAPFIVLHDADIEAAVQAAAFGAFANSGQICMSTERIIVDERVADDF 301 +++K + +ELGG AP +V DAD++ A + A F N+GQ+C+S R +V + V + F Sbjct: 240 GQHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAF 299 Query: 302 VARLVAKARSLPLGDPRQGPVVLGSVVDLATVQRCNALIDDALAKGARLVCGGK---AES 358 V + V A L +G+ + + +G V + + ALIDDA+++GA+L GGK + Sbjct: 300 VDKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKG 359 Query: 359 TLMPATLLDHVTAQMDIYHEESFGPVKPIVRVKDDDAAIACANDNEYGLSAALFSRDIAR 418 T+L ++ Q +EE FGP+ I + D I AN YGL+A F++ Sbjct: 360 YFFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHT 419 Query: 419 ALNLVRRIESGICHIN--GATVHDEAHMPFGGVKSSGFGHFGGTAGVAEFTDLRWIT 473 L R+ESG+ IN G + + +PFGG+K SG+G GGT + + + + +T Sbjct: 420 KHALAARVESGMLTINHLGLALPE---LPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 235 bits (599), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 150/417 (35%), Positives = 223/417 (53%), Gaps = 10/417 (2%) Query: 64 ERRSLLTRAADALQARADAFAEAMASETGASHHWAAFNVHLAADGLREAAAMTTQIAGQL 123 +R ++ R D L A D ++ E G A V AA + T+ G + Sbjct: 74 QRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDI 133 Query: 124 IPSDVPGNLAMGVRQAAGVVLGIAPWNAPVILGVRAVALPLACGNTVIFKGSEICPATHG 183 IP+ V G M +++ GVV I PWN P + R +A LA G TV+ K +E P T Sbjct: 134 IPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSL 193 Query: 184 LIIDAFQDAGLPPGVVNFITNAPEDAGKIVEAIIADPAVRRVNFTGSTRVGRIIAGQCAK 243 ++ Q+AG+P GV+N +T + E ++V+ +AD VR+++FTGST VG+ +A A Sbjct: 194 ALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSAD 253 Query: 244 YLKPSVLELGGKAPFIVLHDADIEAAVQAAAFGAFANSGQICMSTERIIVDERVADDFVA 303 LK LELGG APFIV DAD++AA+ F N GQ C+S RI V E+V D FV Sbjct: 254 TLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVD 313 Query: 304 RLVAKARSLPLGDPRQGPVVLGSVVDLATVQRCNALIDDALAKGARLVCGGK-------- 355 +L A+ +L +G +G +++ + + + + DA+A+GAR++ GGK Sbjct: 314 KLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFG 373 Query: 356 AESTLMPATLLDHVTAQMDIYHEESFGPVKPIVRVKDDDAAIACANDNEYGLSAALFSRD 415 +++ P L D VT M EE+FGPV PI R +D IA AN +GL+A +S D Sbjct: 374 SDNYYAPTVLAD-VTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTD 432 Query: 416 IARALNLVRRIESGICHINGATVHDEAHMPFGGVKSSGFGHFGGTAGVAEFTDLRWI 472 + R + +ESGI +N + EA PFGGVK SG+G G G+ ++ +++ Sbjct: 433 VRRIHRVTDALESGIVGVNEGALAAEA-APFGGVKESGYGREGSVHGLDDYLHTKYV 488 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,378,922 Number of extensions: 56375 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 20 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)