BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4317.p (459 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 743 0.0 gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 183 3e-47 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 166 3e-42 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 120 2e-28 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 100 3e-22 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 743 bits (1917), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/458 (81%), Positives = 405/458 (88%) Query: 1 MSTYGIDQAKVVDIGRVLDEGTFTGFQKMVVILAALSIVMDGFDGQLIGFAIPSLIKEWG 60 MS G + VDIGR+LD+G F+GFQK+VV LAALSIVMDGFDGQLIGFAIPSLIKEWG Sbjct: 1 MSKLGAHGNQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWG 60 Query: 61 VTRNAFAPAVAAGLVGMGLGSAFAGLFADRFGRRLAIIGSVFVFGIATSLISLSPDITTI 120 +TRNAFAPAVAAGLVGMG+GSAFAGLFADRFGRR+AIIGSVFVFG+ATSLI+ SPD+TTI Sbjct: 61 ITRNAFAPAVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTI 120 Query: 121 AILRFFAGLGIGGALPVSTTITAEFSPARIRTFAVTATIVCVPLGGMLAGLFANLVLPHY 180 AILRF AGLGIGGALP STT+TAEFSPAR RT AVTATIVCVPLGGMLAGLFAN VLPHY Sbjct: 121 AILRFCAGLGIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHY 180 Query: 181 GWRGLFLLGGMLPVAFSLLLWFCIPESPRYLARQPDRWDELRSLLTRMSRTISAGMRFAD 240 GWRGLFL+GG+LPVAFS+LLWF IPESPRYLAR P RWDELR LL RMSR +SA FAD Sbjct: 181 GWRGLFLIGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFAD 240 Query: 241 AREQNSQPKAGFGALFAEGRARDTIAIWLAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAA 300 AREQ + +AGFGALFA G+ARDT+A+W+AFFMNLLAVYSAFSWLPTMLAGEGLSPSVA Sbjct: 241 AREQAIETRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAG 300 Query: 301 NGLTAYNIGGVFGALLCAVVIGRFGSRVPMLLCCLAAAGTAFALRSLDVSSQTTLLVAGI 360 GLTAYN GGVFGALLCA+ I RFGSR+PMLLCCLAA +A LRSLDV+SQTTLLV GI Sbjct: 301 AGLTAYNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGI 360 Query: 361 GLHGLFVNAVQCTMYALCAYIYPTQVRATGTAGALAFGRLGAILSAFAGAIVITQGGASG 420 GLHGLFVNAVQ TMYALCAYIYPTQVRATGTA ALAFGRLGAILSAFAGA+VITQGGA+G Sbjct: 361 GLHGLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVITQGGAAG 420 Query: 421 YLAMLGGAMLFVFVTLLMVSKHIPRAAATVKHQEKEQQ 458 YLAML GAML VFV LL+V KHIPR A K++ Sbjct: 421 YLAMLAGAMLMVFVALLVVRKHIPRVNAGAAETLKKES 458 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 183 bits (464), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 131/423 (30%), Positives = 209/423 (49%), Gaps = 22/423 (5%) Query: 1 MSTYGIDQAKVVDIGRVLDEGTFTGFQKMVVILAALSIVMDGFDGQLIGFAIPSLIKEWG 60 MS + V + +V+++G F FQ +++L L +++DGFD Q +G+ P++I +WG Sbjct: 1 MSIHTSGALPTVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWG 60 Query: 61 VTRNAFAPAVAAGLVGMGLGSAFAGLFADRFGRRLAIIGSVFVFGIATSLISLSPDITTI 120 V++ + P AGL GM LGS DR+GRR +I S F + + + + Sbjct: 61 VSKASLGPVFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQL 120 Query: 121 AILRFFAGLGIGGALPVSTTITAEFSPARIRTFAVTATIVCVPLGGMLAGLFANLVLPHY 180 +LRF G G+G +P + + EFSP+ R + +G G + ++P Y Sbjct: 121 LVLRFITGFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAY 180 Query: 181 GWRGLFLLGGMLPVAFSLLLWFCIPESPRYLA--RQPDRWDELRSLLTRMSRTISAGMRF 238 GW +F +GG +P+ L + +PES ++L R+P R+ + R R + + Sbjct: 181 GWHAVFWVGGAVPLVLGLAMLVWLPESIQFLVLHRRP------RTQVARWLRKLDPNIVI 234 Query: 239 ADAREQNSQPKAGFG----ALFAEGRARDTIAIWLAFFMNLLAVYSAFSWLPTMLAGEGL 294 D E + G ALF +GRA T+ +WL FMNL+ +Y +WLPT++ G Sbjct: 235 DDKTEVVVKETKAEGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGY 294 Query: 295 SPSVAANGLTAYNIGGVFGALLCAVVIGRFG-SRV---PMLLCCLAAAGTAFALRSLDVS 350 S S+A T+ +GGV G L I RFG +RV LL CL+ A ++ Sbjct: 295 STSMAVLIGTSLQVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIG------KLA 348 Query: 351 SQTTLLVAGIGLHGLFVNAVQCTMYALCAYIYPTQVRATGTAGALAFGRLGAILSAFAGA 410 + LL + + G + Q + AL YPT +R+TG AL GR+G+++ G Sbjct: 349 TVPALLFVAVIVAGFCIVGGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGG 408 Query: 411 IVI 413 +I Sbjct: 409 QLI 411 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 166 bits (421), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 133/404 (32%), Positives = 202/404 (50%), Gaps = 13/404 (3%) Query: 10 KVVDIGRVLDEGTFTGFQKMVVILAALSIVMDGFDGQLIGFAIPSLIKEWGVTRNAFAPA 69 K +D+ +++D FT F MV+ L AL +V DG+D + G +P L+KEW ++ Sbjct: 2 KTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGAL 61 Query: 70 VAAGLVGMGLGSAFAGLFADRFGRRLAIIGSVFVFGIATSLISLSPDITTIAILRFFAGL 129 + L GM G+ G ADR GR+ + S +F ++T L + T + RF AGL Sbjct: 62 GSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGL 121 Query: 130 GIGGALPVSTTITAEFSPARIRTFAVTATIVCVPLGGMLAGLFANLVLPHYGWRGLFLLG 189 G GG +P + + E++P R+R+ V LGG+L +LP++GW+ +F Sbjct: 122 GCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAA 181 Query: 190 GMLPVAFSLLLWFCIPESPRYLARQPDRWDELRSLLTRMSRTISAGMRFADAREQNSQPK 249 + V ++LW +PES +L RQ R ++ R++LT+ + R A + Sbjct: 182 AVPLVLLPVILW-KLPESVGFLLRQ-GRLEQARAMLTQ----VDPQARIATDTQLVQADV 235 Query: 250 AGFGA----LFAEGRARDTIAIWLAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAANGLTA 305 G GA LF + RA T+ IW++FF LL VY+ SWLP ++A G S + + L A Sbjct: 236 KGAGAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLA 295 Query: 306 YNIGGVFGALLCAVVIGRFGSRVPMLLCCLAAAGTAFALRSLDVSSQTTLLVAGIGLHGL 365 N GGV GA+ + RF +P ++ GT A+ L +S T +L I + G Sbjct: 296 LNFGGVVGAIGGGWLGDRF--TLPKVVVSFFTVGTV-AIALLGFNSPTPVLYLLIIVAGA 352 Query: 366 FVNAVQCTMYALCAYIYPTQVRATGTAGALAFGRLGAILSAFAG 409 Q +YA A YP +R+TG A GR GAI+ G Sbjct: 353 TTIGTQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLG 396 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 120 bits (301), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 119/398 (29%), Positives = 185/398 (46%), Gaps = 29/398 (7%) Query: 26 FQKMVVILAALSIVMDGFDGQLIGFAIPSLIKEWGVTRNAFAPAVAAGLVGMGLGSAFAG 85 FQ + ++ L + D IGF+ P++ K +G+T VGM LG+ G Sbjct: 20 FQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFG 79 Query: 86 LFADRFGRRLAIIGSVFV---FGIATSLISLSPDITTIAILRFFAGLGIGGALPVSTTIT 142 ADR GRR ++G+V + FG+A++ +PD T + LR G+G+GG LPV T+ Sbjct: 80 RLADRIGRRPVLMGAVVIDACFGVASAF---APDFTWLLALRLLTGIGVGGTLPVDYTMM 136 Query: 143 AEFSPARIR--------TFAVTATIVCVPLGGMLAGLFANLVLPHYGWRGLFLLGGMLPV 194 AEF P+ R +F TI C+ + ++A + N WR +F + G LP Sbjct: 137 AEFLPSARRGRWLVMLESFWAIGTI-CLAVLALVAVSWGN-----DAWRVIFFVTG-LPA 189 Query: 195 AFSLLLWFCIPESPRYLARQPDRWDELRSLLTRMSRTISAGMRFADAREQNSQPKAGFGA 254 ++L F +PESP YL R + D R +L R+++ + + + + K A Sbjct: 190 LVGVVLRFYVPESPMYLNRS-GKSDAARQVLERVAKVNGSSTPIPPLQPEVVERKP-MSA 247 Query: 255 LFAEGRARDTIAIWLAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAANGLTAYNIGGVFGA 314 LF+ R +++++LA+ + +A Y F +LP L EG L + + G Sbjct: 248 LFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLALVQLPGY 307 Query: 315 LLCAVVIGRFGSRVPMLLCCLAAAGTAFALRSLDVSSQTTLLVAGIGLHGLFVNAVQCTM 374 L A + R+G R P L+ L + L SL L+V L + + Sbjct: 308 ALSAYGVERWG-RKPTLVGFLVLSAVGCMLYSL--GKDPMLVVGSTLLLSFSLLGTWAAL 364 Query: 375 YALCAYIYPTQVRATGTAGALAFGRLGAILSAFAGAIV 412 YA +YPT +RA+G A A R G + FA AIV Sbjct: 365 YAFTPEVYPTDLRASGMGTAGAVARFGGL---FAPAIV 399 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 98/406 (24%), Positives = 179/406 (44%), Gaps = 37/406 (9%) Query: 18 LDEGTFTGFQKMVVILAALSIVMDGFDGQLIGFAIPSLIKEWGVTRNAFAPAVAAGLVGM 77 ++ F+ + I+ + + D F + F +P L+ W ++ +A+G +G Sbjct: 33 IENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQ 92 Query: 78 GLGSAFAGLFADRFGRRLAIIGSVFVFGIATSLISLSPDITTIAILRFFAGLGIGGALPV 137 +G+ F G A+R+GR + +V + + + + +L+ + + RF G+G+GG +PV Sbjct: 93 VIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPV 152 Query: 138 STTITAEFSPARIRTFAVTATIVCVPLGGMLAGLFANLVLPHYGWRGLFLLGGMLPVAFS 197 + T E S A R + PLG + A ++P +GW +FL+GG+ + + Sbjct: 153 AATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLVGGIPGILVA 212 Query: 198 LLLWFCIPESPRYLARQPDRWDELRSLLTRMSRTISAG--MRFADAR------EQN---- 245 LL+ +PESPR+L + R D+ L+ RT+ A R DA+ EQ Sbjct: 213 LLI-TRLPESPRWLIAR-GRLDDAEKLI----RTLEASTPKRNLDAQTDKQAIEQRINAL 266 Query: 246 -----SQPKAGFGALFAEGRARDTIAIWLAFFMNLLAVYSAFSWLPTMLA-------GEG 293 + + + LF+ TI +WL + + +WLP++ E Sbjct: 267 AAGAGKRQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSLYKTVYHLPLQES 326 Query: 294 LSPSVAANGLTAYNIGGVFGALLCAVVIGRFGSRVPMLLCCLAAAGTAFALRSLDVSSQT 353 L + +N L+A + G CA++I R G R + + + L L S Sbjct: 327 LRMASVSNVLSACAVLG------CALLIDRVGRRRWAIASFILSGALLALLGVLKAESAW 380 Query: 354 TLLVAGIGLHGLFVNAVQCTMYALCAYIYPTQVRATGTAGALAFGR 399 +++ + + + +Y IYPT++RA GT A ++ R Sbjct: 381 SVMFLASSAYAV-MGTTTVLLYLYTPEIYPTRMRAIGTGLATSWLR 425 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,437,478 Number of extensions: 63336 Number of successful extensions: 512 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 479 Number of HSP's successfully gapped: 30 Length of query: 459 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 371 Effective length of database: 1,203,190 Effective search space: 446383490 Effective search space used: 446383490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)