BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4316.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [... 423 e-120 gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herba... 90 3e-19 gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [... 88 8e-19 >gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 423 bits (1088), Expect = e-120, Method: Compositional matrix adjust. Identities = 204/301 (67%), Positives = 248/301 (82%) Query: 1 MNIAIVGAGAMGGLFGARLALNGQKVSFIEVAPATIAAISDNGLAFSDDSGTHTVRVPVG 60 M IAIVGAGAMGGLF ARLAL GQ VS IEVAPA+I AIS +GL+ +D+ G HTVRVPVG Sbjct: 1 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG 60 Query: 61 RADSFAEPFDLIVFFTKSLHTQSAVRSISHLVGQDTWALSVQNGLGNAEIISEVIPSERI 120 RADSF + FDLI+ FTK HTQ+A+ S++HL G TWALSVQNGLGNA++I+EVIP ERI Sbjct: 61 RADSFQDAFDLIILFTKGFHTQAAIASVAHLAGPHTWALSVQNGLGNADLIAEVIPRERI 120 Query: 121 IVGMTNLPADLKSAGVVHSHGEGYVRIWTMSGKERPEVSRVAEIFSAAGLNCSADPQVRA 180 IVGMTNLPADL++ GVVHSHGEG VRIWT+SG+ ++++VA F+AAGL CSADP V+ Sbjct: 121 IVGMTNLPADLRAPGVVHSHGEGQVRIWTLSGQPSAQLTQVAADFNAAGLPCSADPLVQQ 180 Query: 181 AIWEKVAFNAALNSVCAVIGCTVGTAGSTEQGRALAMRIAAETVSVAQALQIPARIAKVE 240 AIWEK+AFNAALN++CAV G TVG AG++ GR LA+RIAAETV+VAQA I AR+ +VE Sbjct: 181 AIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIAAETVAVAQAAGIDARLVQVE 240 Query: 241 EALLDAFRMHVNHKPSMLQDIEAGRQTEIDFINGAVAEQARGCGVSVPTVTALYELVKMK 300 +A+ AFR H++HKPSMLQDIEAGR+TEIDFINGAV +A G+ VPT L++LVK+ Sbjct: 241 DAIEHAFRTHLHHKPSMLQDIEAGRRTEIDFINGAVVREAERLGLPVPTTQTLHQLVKLM 300 Query: 301 Q 301 + Sbjct: 301 E 301 >gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 89.7 bits (221), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 88/333 (26%), Positives = 145/333 (43%), Gaps = 34/333 (10%) Query: 1 MNIAIVGAGAMGGLFGARLALNGQKVSFIEVAPATIAAISDNGLAFSDDSGTHTVRVPVG 60 + I I GAGA+GG RLA G +VS + T+ AI ++GL D GT R Sbjct: 15 LRICIAGAGAIGGTLAVRLAAAGHQVSVLARG-QTLQAIREHGLTLHDLHGTAHARPAAD 73 Query: 61 RADSFAEPFDLIVFFTKSLHTQSAVRSISHLVGQDT-----------WALSVQNGLGNAE 109 SF D+I KS + ++ +VG+ T W + G + + Sbjct: 74 HRPSFGVQ-DVIFLCAKSQDMAGLLPQVAPMVGETTVVIPTNNGVPWWYFHREGGRFDGQ 132 Query: 110 IISEVIPS---------ERIIVGMTNLPADLKSAGVVHSHGEGYVRIWTMSGKERPEVSR 160 + V P +II + + A+++SAGV+ S + + SG+ + R Sbjct: 133 TVKAVDPEGALAAALPLAQIIGSVLFITAEVESAGVIRSVNPHLMVLGEPSGEMSERLLR 192 Query: 161 VAEIFSAAGLNCSADPQVRAAIWEKVAFNAALNSVCAVIGCTVGTAGSTEQGRALAMRIA 220 V +AG+ A ++R +W K+ N + N + + T+ + R + +I Sbjct: 193 VRAAVESAGIEARATDRIRDKLWTKIIANISTNPLSVITQGTLQQLYGLPELREVVSQIM 252 Query: 221 AETVSVAQA----LQI-PARIAKVEEALLDAFRMHVNHKPSMLQDIEAGRQTEIDFINGA 275 ET+ VA + + I P ++ EA + AFR SMLQD+E GR E+ I A Sbjct: 253 RETLLVASSHGARVDIDPLTFLQLGEA-MGAFRT------SMLQDLERGRPLELAAIGDA 305 Query: 276 VAEQARGCGVSVPTVTALYELVKMKQDAVLSRK 308 V E A + +P A+ L + + +A ++ Sbjct: 306 VLEMAERYAIPMPITRAIVSLARFRGEAAQQQR 338 >gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 88.2 bits (217), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 84/314 (26%), Positives = 145/314 (46%), Gaps = 33/314 (10%) Query: 1 MNIAIVGAGAMGGLFGARLALNGQKVSFIEVAPATIAAISDNGLAFSDDSGTHTVRVPVG 60 M +A++GAGA+G F LA G +V+ + P + A + GL + T+ V Sbjct: 1 MKVAVMGAGAVGCYFAGMLARVGHQVALVG-RPQHVEAFNTRGLRLETKAFDETIAVKAS 59 Query: 61 RADSFAEPFDLIVFFTKSLHTQSAVRSISHLVGQDTWALSVQNGLGNAEIISEVIPSERI 120 + A +L++F KS T++A ++ + DT LS+QNG NAE + + ++ + Sbjct: 60 TDAAIAADAELVLFCVKSPDTETAGAQLAPHLAPDTLVLSLQNGTDNAERL-RTVMAQPV 118 Query: 121 IVGMTNLPADLKSAGVVHSHGEGYVRIWTMSGKERPEVSRVAEIFSAAGLNCSADPQVRA 180 + + ++ G V HG G + I +G A+ AAG+ VR Sbjct: 119 AAAVVYVATEMAGPGHVKHHGRGELVIEPAAGSR-----DAADALVAAGVPTEISDNVRG 173 Query: 181 AIWEKVAFNAALNSVCAVIGCTVGT-----------AGSTEQGRALAMRIAAETVSVAQA 229 +W K+ N A N++ A+ G A + +A+A AAE V++A Sbjct: 174 ELWLKLILNCAYNALSAIAQLPYGKLVEGPGIPEVIAQLVTECKAVA---AAEGVTLAD- 229 Query: 230 LQIPARIAKVEEALLDAFRMHVNHKPSMLQDIEAGRQTEIDFINGAVAEQARGCGVSVP- 288 + + + K+ + + F S QD+ G+ +EID +NG + +A+ G+S P Sbjct: 230 -DVDSAVRKIVQTMPGQFS-------STAQDLARGKPSEIDHLNGFIVARAQAHGLSAPA 281 Query: 289 --TVTALYELVKMK 300 TV AL +L++ K Sbjct: 282 NLTVHALVKLIESK 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 932,883 Number of extensions: 38341 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 3 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)