BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4280.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313368|ref|YP_003777460.1| hypothetical protein Hsero_4082... 128 3e-31 >gi|300313368|ref|YP_003777460.1| hypothetical protein Hsero_4082 [Herbaspirillum seropedicae SmR1] Length = 179 Score = 128 bits (321), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 69/171 (40%), Positives = 102/171 (59%), Gaps = 1/171 (0%) Query: 1 MPTKLHEALAVIFQPMAGRARKIIAVPRGVAEELPFVNSVAIISITARDKSLATLAPFPH 60 M ++ + A I +P ++++A+ +G AE +P + VA+ISI D + A L P+ Sbjct: 1 MSQRIKDIFAAIHRP-PETGKRVLALSKGDAERIPLLPGVAMISIVDADSAQAVLPPYEF 59 Query: 61 ILRLSFADIDHLNPSASKRSLAKAADAFTPAQASDVLDFVEGLPDSINSVVVHCEGGYSR 120 +LRLSF+D+D L + S R+ K +A T AQ +V FV LP+SI++V+VHC G SR Sbjct: 60 LLRLSFSDVDFLAHNLSDRAQKKLGEAMTKAQGLEVNRFVRALPESIHTVIVHCGAGVSR 119 Query: 121 SCAIAKGLNQIYGFEVREGDLSEANPSVVEVMKRAAASRQRGKRANRRRPK 171 SC +A LN IYGFEV E L AN SV +++ A ++ R K R + K Sbjct: 120 SCGVASALNAIYGFEVEEQRLKGANQSVKKLLIEVAKNQSRSKDNRRAKSK 170 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 522,519 Number of extensions: 19790 Number of successful extensions: 58 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 58 Number of HSP's successfully gapped: 1 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)