BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4268.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogena... 487 e-139 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 92 5e-20 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 74 1e-14 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 72 4e-14 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 69 5e-13 >gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 487 bits (1253), Expect = e-139, Method: Compositional matrix adjust. Identities = 238/255 (93%), Positives = 248/255 (97%) Query: 6 MTSPSSAGVALVTGAARRIGRHIALALAQAGWDVAVHYNRSRQEAEELVREIEDLGRRAL 65 MTSPSSAG+ALVTGAARRIGRHIALALAQ+GWDVAVHYNRSR+EA+ LVREIE LGRRAL Sbjct: 1 MTSPSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIETLGRRAL 60 Query: 66 AVQGELGDQAQVRALLPQAAPLGRISCVVNNASLFDYDDAASFGNDRLLRHMSANVGAPV 125 AVQ ELGD+ QVR+LLPQAAPLGRISCVVNNASLFDYDDAASFGN+RLLRHMSANVGAPV Sbjct: 61 AVQAELGDETQVRSLLPQAAPLGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPV 120 Query: 126 LLAQALFEITPAGEQAVVVNLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV 185 LLAQAL+E TP G+QAVV+NLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV Sbjct: 121 LLAQALYEATPQGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV 180 Query: 186 VGVAPGLTMVSGDQTEEQFGKAHTVTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG 245 VGVAPGLTMVSGDQTEEQFGKAH VTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG Sbjct: 181 VGVAPGLTMVSGDQTEEQFGKAHQVTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG 240 Query: 246 QHLLPLSRDVMFVAK 260 QHLLPLSRDVMFVAK Sbjct: 241 QHLLPLSRDVMFVAK 255 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 91.7 bits (226), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 79/246 (32%), Positives = 129/246 (52%), Gaps = 14/246 (5%) Query: 14 VALVTGAARRIGRHIALALAQAGWDVAVHYNRSRQEAEELVREIEDLGRRALAVQGELGD 73 V LVTG +R IG AL Q G+ VAV+Y +R A+ +V +I+ G RA+AV G++ Sbjct: 7 VILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGGDVAR 66 Query: 74 QAQVRALLPQA-APLGRISCVVNNASLFDYDDA-ASFGNDRLLRHMSANVGAPVLLA-QA 130 + V L LGR++ +VNNA + D+ +R+ R + ANV +L A +A Sbjct: 67 EEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLCAREA 126 Query: 131 LFEITP--AGEQAVVVNL--LDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK-VRV 185 + ++ G+ +VNL + KL P +++ Y SK A+ + T LA+ +A + +RV Sbjct: 127 VKRMSTRHGGQGGAIVNLSSMAAKLGGPG-EYVDYAASKGAIDAFTIGLAKEVAAEGIRV 185 Query: 186 VGVAPGLTMVSGDQTEEQFGKAHTV---TPLGRGSTPEDIAAAVCYVASARA--VTGTTL 240 V PGL + + G+ + P+ RG + ++A A+ ++ S A TGT + Sbjct: 186 NAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATGTFI 245 Query: 241 LVDGGQ 246 V GG+ Sbjct: 246 DVSGGR 251 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 73.9 bits (180), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 74/244 (30%), Positives = 112/244 (45%), Gaps = 6/244 (2%) Query: 7 TSPSSAGVALVTGAARRIGRHIALALAQAGWDVAVHYNRSRQEAEELVREIEDLGRRALA 66 TS + VA V G +R IG I LA G VA Y S +A+ LV ++ G RALA Sbjct: 12 TSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALA 71 Query: 67 VQGELGDQAQVRALLPQAAP-LGRISCVVNNASLFDYDDAASFGNDRLLRHMSANVGAPV 125 +Q + D A ++ + AA GR+ +VNNA + F + L R ++ NV + Sbjct: 72 IQADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVF 131 Query: 126 LLAQALFEITPAGEQAVVVNLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK-VR 184 + Q G + + + + P Y +SK+AL T LA+ L P+ + Sbjct: 132 VATQEAARYMGEGGRVISIGSTNADRI-PFAGGAVYAMSKSALVGLTKGLARDLGPRGIT 190 Query: 185 VVGVAPGLTMVSGDQTEEQFGKAHT-VTPLGRGSTPEDIAAAVCYVASARA--VTGTTLL 241 V V PG + F + T + L R E+IA+ V Y+A + +TG L+ Sbjct: 191 VNNVQPGPVDTDMNPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITGANLM 250 Query: 242 VDGG 245 +DGG Sbjct: 251 IDGG 254 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 72.4 bits (176), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 71/245 (28%), Positives = 118/245 (48%), Gaps = 10/245 (4%) Query: 14 VALVTGAARRIGRHIALALAQAGWDVAVHYNRSRQEAEELVREIEDLGRRALAVQGELGD 73 V ++GA+ IG A A A G + VHYN S +EAE + E+ G +++ + ++ D Sbjct: 8 VVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITIGADVRD 67 Query: 74 QAQVRALLPQA-APLGRISCVVNNASLFDYDDAASFGNDRLLRH-MSANVGAPVLLAQAL 131 + + A + +A A +GRI ++NNA D L ++ N + V +A+ Sbjct: 68 TSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAFTRAV 127 Query: 132 FEITPAGEQAVVVNLLD-QKLYNPNPDFLSYTLSKAALHSATTLLAQA-LAPKVRVVGVA 189 + A ++N+ + P L Y SK + + T +A+ LA K+RV VA Sbjct: 128 IPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRVNAVA 187 Query: 190 PGLTMVSGDQ---TEEQFGKAHTVTPLGRGSTPEDIAAAVCYVASARA---VTGTTLLVD 243 PG+ M + T EQ P+GR P++ + A Y+AS + VTG + V+ Sbjct: 188 PGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQIIDVN 247 Query: 244 GGQHL 248 GGQ++ Sbjct: 248 GGQYM 252 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 68.6 bits (166), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 74/246 (30%), Positives = 116/246 (47%), Gaps = 28/246 (11%) Query: 16 LVTGAARRIGRHIALALAQAGWDVAVHYNRSRQEAEELVREIEDLGRRALAVQGELGDQA 75 VTG++R IGR IAL LA+ G+D+ +H + R A+++ EI LGR+ +Q ++ ++ Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 Query: 76 Q-VRALLPQAAPLGRISCVVNNASLF-----------DYDDAASFGNDRLLRHMSANVGA 123 Q + LL G VV NA + D+D D NV Sbjct: 70 QAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFY-----NVLN 124 Query: 124 PVLLAQALFEITPAGEQAVVVNLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK- 182 P+++ + AG +V L N ++Y+ +KA + AT LA LA + Sbjct: 125 PLVM--PMVRRRAAGR---IVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRD 179 Query: 183 VRVVGVAPGLTMVSGDQTEEQ-FGKAHTVTPLGRGSTPEDIAAAVCYVASARA--VTGTT 239 + V VAPGL + D TE+ + + P R PE++AAAV ++ S A +T Sbjct: 180 ITVNCVAPGL--IDTDMTEDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQV 237 Query: 240 LLVDGG 245 + V+GG Sbjct: 238 ISVNGG 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 806,207 Number of extensions: 33401 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 16 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)