BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4264.p (451 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 791 0.0 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 190 2e-49 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 173 2e-44 gi|300311751|ref|YP_003775843.1| signal transduction histidine k... 166 5e-42 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 158 1e-39 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 791 bits (2042), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/441 (94%), Positives = 423/441 (95%) Query: 1 MKSIRNKLLLWLGLGLFSIIVLAGVALYFQARGEANQIFDYQMRQIAASLPRQAFSPISP 60 M S+RNKLLLWLGLGLFSIIVLAGVALYFQARGEANQIFDYQMRQIAASLPRQAFSPISP Sbjct: 1 MNSVRNKLLLWLGLGLFSIIVLAGVALYFQARGEANQIFDYQMRQIAASLPRQAFSPISP 60 Query: 61 GQQFPELEDEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGMWRVYSAQIGST 120 GQQFPELEDEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGMWRVYSAQIGST Sbjct: 61 GQQFPELEDEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGMWRVYSAQIGST 120 Query: 121 VVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAEVAARDM 180 VVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAEVAARDM Sbjct: 121 VVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAEVAARDM 180 Query: 181 NSLQPLSDVGMPQEIQPLTHALNDLLARLKHSTETQRAFVADAAHELRTPLTALRLQAQL 240 NSLQPL+DVGMPQEIQPLTHALNDLLARLKH+TETQRAFVADAAHELRTPLTALRLQ QL Sbjct: 181 NSLQPLADVGMPQEIQPLTHALNDLLARLKHATETQRAFVADAAHELRTPLTALRLQTQL 240 Query: 241 AERATDEVERRAAFADLKSGLERATHLVHQLLTLARQEPDAMNFAEVDLRALLGSVVGDL 300 AERA DE ERRAAFADLK+GLER THLVHQLLTLARQEPDAM+F +VDLRALLGSVVGDL Sbjct: 241 AERAVDEEERRAAFADLKNGLERTTHLVHQLLTLARQEPDAMSFTQVDLRALLGSVVGDL 300 Query: 301 SSVAEHGSIDLGLSDQGDDGRPLRVQGNADALRILFNNLVDNALRYTPAGGVVDVGFDLS 360 SSVAEHG IDLGLSDQGDDGRPLRV GNADALRILFNNLVDNALRYTPAGGVVDVG DLS Sbjct: 301 SSVAEHGGIDLGLSDQGDDGRPLRVTGNADALRILFNNLVDNALRYTPAGGVVDVGVDLS 360 Query: 361 TPGACTVVIQDSGPGIAESELPRVFDRFYRAQRGHDQGGSRAAFGSGLGLAIVRQIADLH 420 T G CTVV+QDSGPGIAESELPRVFDRFYRAQ + GSRAAFGSGLGLAIVRQIADLH Sbjct: 361 TAGYCTVVVQDSGPGIAESELPRVFDRFYRAQHSQEGSGSRAAFGSGLGLAIVRQIADLH 420 Query: 421 RVPVSLRNTGHGLQARVEFPL 441 RVPV LRNTGHGLQARV FPL Sbjct: 421 RVPVELRNTGHGLQARVVFPL 441 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 190 bits (482), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 119/306 (38%), Positives = 172/306 (56%), Gaps = 14/306 (4%) Query: 121 VVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAEVAARDM 180 VQV + R ++A ++ ++P ++ P+ L W A+SRGL+P+ + + AR Sbjct: 54 TVQVGETLEKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQRIRARRP 113 Query: 181 NSLQPLSDVGMPQEIQPLTHALNDLLARLKHSTETQRAFVADAAHELRTPLTALRLQAQL 240 + L P+ +P+EI PL +LND+LARL + Q+ F+ADAAH+++TPL +R+Q++L Sbjct: 114 DDLSPIDSGQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAGMRMQSEL 173 Query: 241 AERATDEVERRAAFADLKSGLERATHLVHQLLTLAR---QEPDAMNFAEVDLRALLGSVV 297 A R D+V+ R + L E AT LV+QLL+LAR Q+ + V+L L SVV Sbjct: 174 ALRQDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELARSVV 233 Query: 298 GDLSSVAEHGSIDLGLSDQGDDGRPLRVQGNADALRILFNNLVDNALRYTPAGGVVDVGF 357 D ++ IDLG +Q D P+ + GN LR L +NL+DNAL YTP G V Sbjct: 234 QDWVPMSFTQRIDLGF-EQPD--HPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTV 290 Query: 358 DLSTP---GACTVVIQDSGPGIAESELPRVFDRFYRAQRGHDQGGSRAAFGSGLGLAIVR 414 T G + ++D+GPGI +E VF+RFYR G G GLGLAIVR Sbjct: 291 RARTDEGGGLAILEVEDNGPGIPVAERANVFERFYRI-----LGTPSTGSGLGLGLAIVR 345 Query: 415 QIADLH 420 +IA H Sbjct: 346 EIAQQH 351 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 173 bits (439), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 148/469 (31%), Positives = 214/469 (45%), Gaps = 51/469 (10%) Query: 3 SIRNKLLLWLGLGLFSIIVLAGVALYFQARGEANQIFDYQMRQIAASL-----PRQAFSP 57 SIR LL WL L + ++ Y+ A A FD + L RQ + Sbjct: 7 SIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRHRQERTT 66 Query: 58 ISPGQQFPEL-----EDEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGMWR- 111 QQ ++ D + ++ G ++ +P A F +G R Sbjct: 67 AELTQQAEQILRSNHSDTTFFAVRNTEGQIVVGDKGFPQLP--ASDVFDRPINYDGEMRG 124 Query: 112 ------VYSAQIGSTVVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSW------- 158 ++ + V+ VA ++ R A + LL L G L++ Sbjct: 125 EPVRIAAMQVRVKNGVIWVAVAETIRKRDRAHYTI-----LLSFLVLDGALTFASIFVVL 179 Query: 159 VAVSRGLAPVRKAAAEVAARDMNSLQPLSDVGMPQEIQPLTHALNDLLARLKHSTETQRA 218 VAV RGL P++ + RD L + G P E+QPL A+N+L+AR+ + Q+ Sbjct: 180 VAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQQN 239 Query: 219 FVADAAHELRTPLTALRLQAQ-LAERATDEVERRAAFADLKSGLERATHLVHQLLTLARQ 277 F+AD AH+LRTPLT L+LQ + L ++ D+ + + A + S +ER QLL LAR Sbjct: 240 FMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALARS 299 Query: 278 EP---DAMNFAEVDLRALLGSVVGDLSSVAEHGSIDLGLSDQGDDGRPLRVQGNADALRI 334 EP ++ E+ L L+ V A+ IDLG D RP R++G+ L Sbjct: 300 EPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGF-----DLRPARLRGDRFLLSD 354 Query: 335 LFNNLVDNALRYTPAGGVVDVGFDLSTPGACTVVIQDSGPGIAESELPRVFDRFYRAQRG 394 L +NL+DNA+RY+P G V V L A + ++DSGPGIA +FDRFYRA Sbjct: 355 LIDNLIDNAVRYSPPHGTVTVRC-LEEQDATVLSVEDSGPGIAPEHRELIFDRFYRA--- 410 Query: 395 HDQGGSRAAFGSGLGLAIVRQIADLH--RVPVSLRNTGHGLQARVEFPL 441 + GSGLGLAIVR+IA H + V + G G V FPL Sbjct: 411 -----NDKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPL 454 >gi|300311751|ref|YP_003775843.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 166 bits (419), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 134/340 (39%), Positives = 181/340 (53%), Gaps = 19/340 (5%) Query: 92 MPRQAEL--GFANVSGPNGMWRVY-SAQIGSTVVQVAQPQSARDEIAAQMAVKTVVPLLL 148 +P A+L GF + + +RVY A + +AQ R IA + A + + P ++ Sbjct: 101 LPLPADLAEGFHTLLLHHEYFRVYVQALPNGKRLALAQETDLRQHIAQRSAWRALWPFVV 160 Query: 149 LFPLIGVLSWVAVSRGLAPVRKAAAEVAARDMNSLQPLSDVGMPQEIQPLTHALNDLLAR 208 L PL+ + + R AP++ E+ +R + LQP+++ +P+E+Q A+N LLAR Sbjct: 161 LAPLLLLAVQRIIKRLFAPLQALRQEIDSRGEDDLQPIAERLLPREVQAFVAAINGLLAR 220 Query: 209 LKHSTETQRAFVADAAHELRTPLTALRLQAQLAERATDEVERRAAFADLKSGLERATHLV 268 ++ QR FVADAAHELRTPLTAL LQA+ RA R L++GL+RA LV Sbjct: 221 VEAGMAAQRRFVADAAHELRTPLTALSLQAERLGRAPMSETARERLTRLETGLQRARQLV 280 Query: 269 HQLLTLAR-QEPDAMNFAEVDLRALLGSVVGDLSSVAEHGSIDLGLSDQGDDGRPLRVQG 327 QLL LAR Q P L +L V+ +L +A+ IDLGL D R V Sbjct: 281 TQLLALARAQTPAGAALQPCSLHQVLREVIEELLPLAQCKQIDLGL----DGERDACVLT 336 Query: 328 NADALRILFNNLVDNALRYTPAGGVVDVGFDLSTPGACTVVIQDSGPGIAESELPRVFDR 387 L L NL+DNA+ YTPAGG V + + + I D+GPGIA SE RVF Sbjct: 337 QPQELAQLLRNLIDNAIAYTPAGGAVSLRMERHAQW-VALEICDTGPGIAASERERVFQP 395 Query: 388 FYRAQRGHDQGGSRAAFGSGLGLAIVRQIADLHRVPVSLR 427 FYR D G GSGLGLAIV+ IA R+ V+LR Sbjct: 396 FYRI----DPSGQ----GSGLGLAIVQSIA--RRLGVTLR 425 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 158 bits (399), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 125/343 (36%), Positives = 177/343 (51%), Gaps = 24/343 (6%) Query: 112 VYSAQI-GSTVVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRK 170 VY I G ++QV + R+ + ++ T+ L + +L W AV L P+ + Sbjct: 144 VYDDSIRGIALIQVGETMEGRNGMTRKILFDTLWRQGTLVTVAALLVWFAVRFVLRPLMQ 203 Query: 171 AAAEVAARDMNSLQPLSDVGMPQEIQPLTHALNDLLARLKHSTETQRAFVADAAHELRTP 230 EV +R L + +E++PL A+N + RL+ QR F+ADA+H+LRTP Sbjct: 204 LKGEVESRAPTDLSGFDTALVHKEMRPLVQAMNGYMGRLQALISAQRRFIADASHQLRTP 263 Query: 231 LTALRLQAQLAERATDEVERRAA-FADLKSGLERAT----HLVHQLLTLARQEPDAMN-- 283 LT L+ Q++L R + AA DL G+ R T HL ++LLTLAR E A Sbjct: 264 LTVLKTQSELTLRELRREQVDAAELRDLVEGMARTTDAAVHLANRLLTLARAEHGAAEGG 323 Query: 284 FAEVDLRALLGSVVGDLSSVAEHGSIDLGLSDQGDDGRPLRVQGNADALRILFNNLVDNA 343 + L V +LS A I L ++GD + ++ NA L L NL+DNA Sbjct: 324 TMPLSLSDAARQVALELSQQAVSREIGLAFEEEGD----VSIEANALLLHELMVNLLDNA 379 Query: 344 LRYTPAGGVVDVGFDL-STPGACTVV---IQDSGPGIAESELPRVFDRFYRA-QRGHDQG 398 +RYTPAGG V + S+PG TV ++DSGPGI +E +VF+ FYRA H Sbjct: 380 IRYTPAGGHVTLRVRAQSSPGQPTVALLEVEDSGPGIPAAEREKVFEPFYRAGATQHVNP 439 Query: 399 GSRAAFGSGLGLAIVRQIADLHRVPVSLR--NTGHGLQARVEF 439 G G+GLGL+IVR+IA +HR V+L G GL R+ F Sbjct: 440 G-----GTGLGLSIVREIAAMHRASVTLDAPAQGSGLVVRIVF 477 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,305,811 Number of extensions: 54643 Number of successful extensions: 339 Number of sequences better than 1.0e-08: 35 Number of HSP's gapped: 256 Number of HSP's successfully gapped: 35 Length of query: 451 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 363 Effective length of database: 1,203,190 Effective search space: 436757970 Effective search space used: 436757970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)