BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4262.p (616 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 1073 0.0 gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 234 1e-62 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 185 8e-48 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 107 3e-24 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 1073 bits (2775), Expect = 0.0, Method: Compositional matrix adjust. Identities = 574/606 (94%), Positives = 591/606 (97%) Query: 1 MDEQLLIALVYLLAAVAAVPLAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF 60 MDEQLLIALVYLLAAVAAVP+AKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF Sbjct: 1 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF 60 Query: 61 GVVLLLFLIGLELDPKRLWALRRSIFGWGSVQVGAVSLALFAAALAAGVPWRIGLIAALG 120 GVVLLLFLIGLELDPKRLWALRRSIFGWGS+QV AVSLAL AAALAA VPWRIGLI ALG Sbjct: 61 GVVLLLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAALAASVPWRIGLIGALG 120 Query: 121 MSLSSTAIALATLGERKLTATPAGATGFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGW 180 MSLSSTAIALATLGERKLTATPAG+ FSILLFQDIAAIPMMAAVPLLGVAASQGSGQGW Sbjct: 121 MSLSSTAIALATLGERKLTATPAGSAAFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGW 180 Query: 181 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVISTCLLMQSVGMSMA 240 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVI+TCLLMQSVGMSMA Sbjct: 181 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMA 240 Query: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRIIGLVIG 300 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVF AQPWRI+G+V+G Sbjct: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLG 300 Query: 301 FLAIKTAVMWFLGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSLLVV 360 FLAIKT VMW LGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPE SSLLVV Sbjct: 301 FLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVV 360 Query: 361 VVALSMLVTPLLLLVHDRLLVRWLEGGKRRPEDRIGPQDNAVIIAGFGRFGQIIGRLLAA 420 VVALSMLVTPLLLLVHDRLLVRW+EGGKRRPED+I PQD+AVIIAGFGRFGQIIGRLLAA Sbjct: 361 VVALSMLVTPLLLLVHDRLLVRWMEGGKRRPEDKIDPQDHAVIIAGFGRFGQIIGRLLAA 420 Query: 421 NDISATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEM 480 N I ATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEM Sbjct: 421 NGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEM 480 Query: 481 VDKVREEFPHLQIAARARNVTHYYDLMDRGVTILERETFESALHMGRQVLQQLDFGAYRA 540 VDKVREEFPHLQIAARARNVTHYYDLMDRGVT+LERETFESALHMGRQVLQQLDFGAYRA Sbjct: 481 VDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETFESALHMGRQVLQQLDFGAYRA 540 Query: 541 REAAMKFRAHNLKTLAALYPHYKDQAQMVSMAVQAREELQAMFAGDREAMQRDHAHDQWR 600 REAAMKFRAHNLKTL ALYPHYK+QAQMVSMAVQAREEL+AMFAGDR+AM+R+H+HDQWR Sbjct: 541 REAAMKFRAHNLKTLEALYPHYKNQAQMVSMAVQAREELEAMFAGDRQAMEREHSHDQWR 600 Query: 601 GEPPPS 606 GEPPP Sbjct: 601 GEPPPQ 606 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 234 bits (597), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 197/642 (30%), Positives = 309/642 (48%), Gaps = 49/642 (7%) Query: 5 LLIALVYLLAAVAAVPLAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVL 64 L + L L AAV V + + L +LGYL+ GI+IGP GLG + + EFGVV Sbjct: 5 LELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLGFAEDNETTHALGEFGVVF 64 Query: 65 LLFLIGLELDPKRLWALRRSIFGWGSVQVGAVSLALFAAALAA--------GVPWRIGLI 116 L+F IGLE +L A+R +FG G QV A A + W+ Sbjct: 65 LMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQAAFA 124 Query: 117 AALGMSLSSTAIALATLGERKLTATPAGATGFSILLFQDIAAIPMMAAVPLLGVAASQGS 176 +++SSTAI L ER T G +LLFQD+A +P++ VP A + S Sbjct: 125 LGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVP----ALANES 180 Query: 177 GQ-----GWLHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVISTCLL 231 G GW AG+ +V+ALL+F G LMR RI+ K +E+F LL+ + + Sbjct: 181 GNIMVTLGW-AAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWI 239 Query: 232 MQSVGMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQP 291 + G+S+ALG F+AG+L++++E++H +E D++ F+ +LLGLFFI +GM ++ + Sbjct: 240 TEKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHW 299 Query: 292 WRIIGLVIGFLAIKTAVMWFLGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLT 351 + ++ L+ G + +K ++ L R FG G L L+Q GEF F++ A K++ Sbjct: 300 FLVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVD 359 Query: 352 PEISSLLVVVVALSMLVTPLLLLVHDRLLVR-----WLEGG---KRRPEDRIGPQDNAVI 403 P + +++ + LSML TP +L D ++++ W+ + +G Q + VI Sbjct: 360 PMLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKH-VI 418 Query: 404 IAGFGRFGQIIGRLLAANDISATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADK 463 IAGFGR GQ + RLL I+ L+ DP+ + R G V YGDA R + L AG + Sbjct: 419 IAGFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHR 478 Query: 464 ARVLVIAIDDAQAALEMVDKVREEFPHLQIAARARNVTHYYDLMDRGVTILERETFESAL 523 A LV+ +AL+++ E P L + R+ + L+ G T + E E +L Sbjct: 479 AAALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSL 538 Query: 524 HMGRQVLQQLDFGAYRAREAAMKFRAHNLKTLAALYPHYKD-QAQMVSMAVQ-------- 574 + L L R R +L + D +A V M V+ Sbjct: 539 MLASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSDGEADGVGMNVRLQAVTLIG 598 Query: 575 ---------AREELQAMFAGDREAMQRDHAHDQWRGEPPPSS 607 A +L+A + +++R ++ R EPP + Sbjct: 599 GASALGHTLAEIDLRAHCGAEVASLRR----NKQRIEPPEQT 636 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 185 bits (470), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 162/520 (31%), Positives = 259/520 (49%), Gaps = 17/520 (3%) Query: 21 LAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVLLLFLIGLELDPKRLWA 80 LA RL + ++GYL+AGI IGP+ G + + E +E GV+LL+F +GL K L + Sbjct: 24 LAHRLKMPPLVGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLS 83 Query: 81 LRRSIFGWGSVQVGAVSLALFAAALAAGVPWRIGLIAALGMSLSSTAIALATLGERKLTA 140 ++ Q+ +L A G P G + L +S++ST + L L ER+L Sbjct: 84 VKNIAIPGAVAQITIATLLGMALGWLLGWPVGEGFLFGLALSVASTVVLLKALQERRLVE 143 Query: 141 TPAGATGFSILLFQDIAAIPMMAAVPLL-GVAASQG---SGQGWLHA-----GQVALVIA 191 T G L+ +DIA + + +P L G+ +G SG L G+V A Sbjct: 144 TQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGNAVLLTLGITLGKVVAFAA 203 Query: 192 LLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVISTCLLMQSV-GMSMALGTFLAGVLL 250 +++ GR ++ IL IA T RE+F L + + L V G+S ALG F AG++L Sbjct: 204 VMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSFALGAFFAGMIL 263 Query: 251 ADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRIIGLVIGFLAIKTAVMW 310 ++SE H + P + LFF++VGM D + +P ++ V+ + K+ Sbjct: 264 SESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVAREPLLVLATVLIIVVGKSLGAL 323 Query: 311 FLGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSLLVVVVALSMLVTP 370 + R FG P G L + L+Q GEF+FI+ ++ +L+P L++ LS+L+ P Sbjct: 324 LIVRAFGYPNGTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILGGAILSILLNP 383 Query: 371 LLLLVHDRLLVRWLEG--GKRRPEDRIGPQDNAVIIAGFGRFGQIIGRLLAANDISATVL 428 LL + DR R + G P+ ++ QD+ V++ GFGR G+ IG L A V+ Sbjct: 384 LLFSLLDRYEARQPKAPQGGDEPQ-KVELQDH-VVLVGFGRVGRGIGEQLRAQGKPFVVI 441 Query: 429 DHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEMVDKVREEF 488 + + ++ LR G YG+A + +LL+ A D+AR L++AI + A ++++ E Sbjct: 442 EAQLENLEALRAAGVPALYGNAAQSELLQAAAIDRARWLLVAIPEVFEAGQVIENALELN 501 Query: 489 PHLQIAARARNVTHYYDLMDRG---VTILERETFESALHM 525 P LQ+ ARA + L G V + ERE L + Sbjct: 502 PGLQVVARAHSDAEIEHLEKHGAQRVIMGEREIARGMLEL 541 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 107 bits (267), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 106/378 (28%), Positives = 188/378 (49%), Gaps = 9/378 (2%) Query: 7 IALVYLLAAVAAVPLAKRLGLGAVLGYLLAGIVIGPWG--LGLITEVKHILEFSEFGVVL 64 +A++ L+A + V K L VLGY++AG++IGP LI + K + SE GV+ Sbjct: 11 MAIIMLIAGIVTVVFNK-LKQPVVLGYIVAGVIIGPHTPPYDLIQDEKTVHILSELGVIF 69 Query: 65 LLFLIGLELDPKRLWALRRSIFGWGSVQVGAVSLALFAAALAAGVPWRIGLIAALG--MS 122 LLF +GLE K+L + + F + ++ + + L G W+ LG ++ Sbjct: 70 LLFSLGLEFSLKKLAKVGATAFIGAAAEILLMIWIGYEIGLYFG--WKRMDAIFLGAMLA 127 Query: 123 LSSTAIALATLGERKLTATPAGATGFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGWLH 182 +SST I + L E + F IL+ +DI AI M+A + + + S S + + Sbjct: 128 VSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVATSGSVDSTEVFTT 187 Query: 183 AGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVISTCLLMQSVGMSMALG 242 G++ L + + + G + + +L I++ E+ L ++ CLL+ + S+ALG Sbjct: 188 VGKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLVMKLQYSVALG 247 Query: 243 TFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRIIGLVIGFL 302 FL G ++A+S + H +E +EP + + +FF+A+G+ D V L + W I ++ + Sbjct: 248 AFLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNV-LVKYWMPITVITLAV 306 Query: 303 AIKTAVMWFLGRCFGIPRGQQ-LFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSLLVVV 361 + LG G+ + L+Q GEF+FI+ + KV + + ++V V Sbjct: 307 VFGKLISCGLGTFIAGQGGRTPMRVGMGLAQIGEFSFIIAALGQSLKVTSDFLYPIVVAV 366 Query: 362 VALSMLVTPLLLLVHDRL 379 A++ L+TP L+ D L Sbjct: 367 SAVTALLTPYLIKAADPL 384 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.142 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,581,956 Number of extensions: 64408 Number of successful extensions: 359 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 350 Number of HSP's successfully gapped: 4 Length of query: 616 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 525 Effective length of database: 1,188,985 Effective search space: 624217125 Effective search space used: 624217125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)